SOL-GEL법을 이용한 Ta₂O_(5) 감지 게이트 pH-ISFET의 제조와 그 동작특성

장기석,이정철,조병욱,권대혁,김창수,손병기 경북대학교 센서기술연구소 1995 센서技術學術大會論文集 Vol.6 No.1

Low drift pH-ISFET is presented in this paper. Ta_(2)O_(5), known as a good pH sensing membrane, was formed by sol-gel method on Si_(3)N_(4)/SiO_(2) gate of pH-ISFET. It has been reported that the drift of the pH-ISFET is caused by the surface deterioration or hydration of sensing membrane. Hydration is resulted from diffusion of ions into the sensing membrane. It is reported that Ta205 film does not show surface deterioration but hydration while Si_(3)N_(4) film is not hydrated but its surface is deteriorated. Therefore, ultra thin(about 100Å) Ta_(2)O_(5) sensing membrane was formed by sol-gel method on the Si3N4to shorten the hydration time. The fabricated Ta205 pH-ISFET showed good sensitivity(58~59 mV/pH) in a wide pH range(pH3~11) as well as very low drift (0.06 pH/day)

MANNOPROTEIN 과 GLUCAN 의 첨가가 쥐의 성장과 NATURAL KILLER CELL ACTIVITY 에 미치는 영향

손광수,조광근,최윤재,윤연화,김재영,한영근,문태현,김성찬 한국영양사료학회 1999 韓國營養飼料學會誌 Vol.23 No.6

본 연구의 목적은 효모 세포벽, 또는 효모의 세포벽으로부터 본 연구에서 확립한 방법으로 분리한 mannoprotein(MP)과 glucan(Glu)을 동물에 급여하므로써 동물의 일당중체량, 일당사료섭취량과 사료:중체량의 비율, 그리고 동물이 가지고 있는 면역기능의 활성화정도를 조사하는데 있다. 산업적 측면에서 대량 추출하기 위하여 기존에 있는 방법을 개선하여 변형된 방법으로 효모세포벽제제를 분리하였다. 본 연구에서 분리된 효모세포벽제제(cell wall, mannoprotein glucan)와 시판중인 효모세포벽제제(Bio-Mos^(TM))를 0.1%로 4주령 횐쥐에 4주간 급여하였다. 일당중체량은 개선된 방법으로 분리한 glucan을 급여한 처리구에서 가장 높은 중체량을 나타냈으며, 다음으로 mannoprotein 급여구가 높았으며 cell wall과 시제품(Bio-Mos^(TM)) 급여구에서 낮은 중체량을 보였다. 일당사료섭취량에 있어서 glucan 급여구가 cell wall과 시제품 급여구들과 비교할 때 높았으며 사료:중체량 비율 또한 glucan 급여구에서 가장 좋은 경향을 보였다. 사양 4주깨 횐쥐로부터 spleen을 채취하여 YACl 세포에 대한 natural killer cell의 cytotoxicity를 분석한 결과 본 실험실에서 분리한 mannoprotein과 glucan이 면역 증진효과가 큰것으로 보였다. Mannoprotein 급여구와 glucan 급여구에서 대조구와 cell wall 급여구보다 면역 효과가 뚜렷하게 나타났으며(MP, P<0.01; Glu, P<0.05), mannoprotein 급여구가 glucan 급여구보다 natural killer cell의 활성도가 높았다(P<0.01). 결론적으로 효모세포벽, mannoprotein과 glucan을 횐쥐에 급여했을 때 성장능력에 있어서 유의성 있는 효과가 나타나지 않았지만 세포배양수준에서 spleen natural killer cell의 activity를 증가시키는 효과가 있다. The purpose of this study was to investigate the average daily gam, average daily feed intake, feed gain ratio and immune system activation of rats when feeding yeast cell wall, mannoprotein (MP) and glucan (Glu) which were isolated from yeast cell wall. In order to isolate the yeast cell wall products in large scale, we used the improved isolation method basing on the typical one. In this study, yeast cell wall products (cell wall, mannoprotein, glucan) and commercial product (Bio-Mos^(TM)) were fed to 4-week old rats by 0.l% during 4 weeks experimental period. Glucan(extracted from improved method) provided the highest average daily gain. Mannoprotein treated group showed the second highest average daily gain. Cell wall and Bia-Mos^(TM) fed groups showed lower average daily gain. Treatment fed glucan also showed higher average daily feed intake than that of cell wall and commercial Bio-Mos^(TM) groups. And in feed: gain ratio, glucan fed rats showed the respectively good results. At the 4th week of feeding trial, rats' spleens were obtained from all the treatments to evaluate the cytotoxicity of natural killer cell against YACl tumor cell. The results indicated that mannoprotein and glucan had the significant effects on activating immune system. Rats of mannoprotein and glucan treated groups showed the higher immune activity than that of cell wall and control groups (MP, P<0.01; Glu, P<0.05), and natural killer cell activity of mannoprotein fed rats was higher than that of glucan fed rats (P<0.01). In conclusion, although the addition of yeast cell wall, mannoprotein and glucan to the diet of rats did not provide the significant effects on growth performance, it was clear that the mannoprotein and glucan isolated in this study could stimulate the activity of spleen natural killer cell in the cell culture.

육성돈에서 Phytase 첨가가 Extrusion 한 쌀겨의 P 이용성에 미치는 영향

김영길,김인호,손중천,김은주,권기범,문태현 한국영양사료학회 1999 韓國營養飼料學會誌 Vol.23 No.3

본 시험은 육성돈에 있어 extrusion된 쌀겨에 대한 P의 이용성과 extrusion된 쌀겨에 phytase를 첨가하므로서 P의 이용성에 미치는 영향을 구명하고자 영양소 소화 시험을 실시하였다. Extrusion된 쌀겨와 extrusion된 쌀겨에 phytase을 첨가한 구간의 비교는 phytase의 첨가에 의해 건물 소화율이 약간 증가하는 경향을 보였다(P<0.04). N 소화율은 쌀겨구 보다는 extrusion된 쌀겨구에서 약간의 증가를 보였다(P<0.07). Ca(P<0.06)과 P(P<0.01)의 소화율은 모두 extrusion된 쌀겨에 phytase의 첨가수준이 다를 경우 500 unit보다는 1,000 mini에서 더 좋은 효과를 보였다. 분 중 P 배설량(2.72g/d vs 3.89g/d)은 extrusion된 쌀겨보다는 extrusion된 쌀겨에 phytase을 첨가하므로서 줄어들었다(P<0.08). 또한 phytase의 첨가수준이 증가할수록 P의 축적량이 증가함을 알 수 있었다(P<0.04). 한편 Ca 섭취량, 배설량 및 축적량은 모든 처리구 간에 통계적인 유의성을 찾아볼 수 없었다. 그러나 최종 Ca 축적량은 쌀겨에 비해 extrusion된 쌀겨의 경우 15%가 향상되었다(6.64g/d vs 5.77g/d). An experiment was conducted to determine the effects of microbial phytase and feed processing on production and phytate P utilization of pigs. Four crossbred barrows with average initial body weight of 30㎏ were used in 24-d metabolism experiment(4 x 4 latin square) to determine the effect of extruded rice bran and phytase supplementation on nutrient utilization. Digestibility of DM in pigs fed extruded rice bran with phytase improved compared to that in pigs fed extruded rice bran(P<0.04). N digestibility was greater for extruded rice bran than rice bran(P<0.07). However, there was no effect of phytase supplementation to extruded rice bran. Digeshbilities of Ca(P<0.06) and P(P<0.01) were greatest for extruded rice bran with phytase 1,000 unit. Fecal excretion of P(2.72g/d vs 3.89g/d) was less for extruded rice bran with phytase than without phytase(P<0.08). Also, when phytase supplementation was increased in diet, there was increased P retention(P<0.04). There were no treatment effects intake, excretion and retention of Ca. However, Ca retention improved by 15% in extruded rice bran compared to rice bran(6.44g/d vs 5.77g/d).

사료내 어분의 채종박에 의한 부분적 대체가 잉어의 성장 및 오염 부하량에 미치는 영향

손광수,김명곤,김정대 한국영양사료학회 1999 韓國營養飼料學會誌 Vol.23 No.6

채종박으로 잉어사료내 어분 대체시, 잉어의 성장과 질소 및 인의 배설량에 미치는 영향을 조사하기 위하여 개시체중 273g인 이스라엘 잉어(Israeli strain of common carp, Cyprinus carpio)을 사용하여 4주간 사양시험을 실시하였다. 실형사료는 갈색어분 25%인 채종박 무첨가구 (RSM 0%), 채종박 5% 첨가구(RSM 5%), 채종박 10% 첨가구(RSM 10%)를 익스투르젼 가공을 시킨 후 3처리 3반복으로 (25마리/반복) 완전임의 배치하여 실시한 실험결과는 다음과 같다. 1. 채종박 5% 사용구는 채종박 무첨가구에 비교시, 중체량, 사료효율, 일일 성장율, 일일 섭취율 및 단백질 이용효율에 있어서는 유의적인 차이가 나타나지 않았으나(P>0.05) 채종박 10% 사용구는 채종박 무첨가구나 채종박 5% 사용구에 비해 성장과 사료이용 효율이 낮았다(P<0.05). 2. 어체내 수분, 단백질, 지방 함량 간에는 채종박 사용 수준에 관계없이 차이가 나타나지 않았으나 (P>0.05%), 조회분과 인의 함량은 채종박 10%사용구가 타처리구에 비해 낮았다 (P<0.05). 3. 질소 섭취량은 처리구간 차이가 없으나 채종박 10%구가 타처리구에 비해 질소 증가량은 낮았고 질소 축적 효율이 낮아 질소 유출량이 많았으나(P<0.05), 채종박 0%와 채종박 5% 사용구간에는 있어서는 질소 축적효율이나 질소 유출량에 차이가 없었다(P>0.05). 4. 인 섭취량은 처리구간 차이가 없으나 채종박 0%구가 타처리구에 비해 인 증가량이 높았고 인 축적효율이 가장 높아 인 유출량이 낮았으나(P<0.05), 채종박 5%와 채종박 10% 사용구간에 있어서는 인 축적효율에 차이가 없었으나(P>0.05) 인 유출량은 채종박 10%구에서 가장 많았다(P<0.05). Dietary fish meal was partially replaced with rapeseed meal in order to study growth, feed utilization, and excretion of nitrogen (N) and phosphorus (P) of Israeli strain of common carp (Cyprinus carpio) weighing 273 g. Growth trial was conducted for 4 weeks using 3 experimental diets, RSM 0%(basal diet) containing 25% brown fish meal, RSM 5% (20% fish meal + 5% rapeseed meal), and RSM 10% (15% fish meal + 10% rapeseed meal). A total of 225 fish were randomly allocated to 3 treatments with 3 replicates (25 fish/rep). Fish were fed 3 times a day to apparent satiety by hand at water temperature of 24℃ to 28℃. RSM 10% treatment showed less weight gain, feed conversion, daily growth rate, daily feed consumption, and protein efficiency ratio than RSM 0% or RSM 5% (P<0.05), but there were no significant differences in those parameters between RSM 0% and RSM 5%. There were no differences in moisture, protein, and fat content of whole body composition among the treatments, but ash and phosphorus contents in the whole body fed RSM 10% were lower than other treatments (P<0.05). N excretion per ㎏ gain of fish fed the diet RSM 10% was significantly greater than other treatments (P<0.05). P retention of fish fed RSM 0% was higher than other treatments fed rapeseed meals and P excretion per kg gain of fish fed RSM 10% was greater than other treatments (P<0.05).

다공질 박막을 이용한 FET 형 용존 산소 센서의 유속 의존성 개선

김영진,손병기,이영철,정훈 경북대학교 전자기술연구소 2001 電子技術硏究誌 Vol.22 No.2

FET type dissolved oxygen sensor was proposed to overcome the disadvantages of the amperometric Clark sensor. The inherent problem of the proposed sensor, however, is the flow dependence of the sensor performances since the proposed sensor detects the pH change in close proximity to the working electrode. In this study, a hydrodynamic porous thin film which can reduce the flow dependence were proposed the suggested porous thin film were formed onto sensing area and working electrode with mixed polymer matrix of TEOS and DEDMS.

흉막염환자에 있어서 흉막액 Adenosine Deaminase 활성도의 임상적 의의

이장훈,김성규,안철민,장상호,손희영,김기호 대한내과학회 1986 대한내과학회지 Vol.31 No.6

A clinical observasion was done on 145 cases of the various pleural effusions admitted to Yonsei University Hospital from Mar. 1984 to Mar. 1986, The pleural effusions were due to tuberculosis(76 cases), suspected tuberculosis(18 cases), malignancy(16 cases), suspected malignancy(22 cases), empyema or pneumonia(10 cases), miscellaneous (3 cases). We have measured the ADA activity of pleural fluids in each cases and the results were as follows. 1) In the tuberculous pleural effusion, the ADA activity of pleural fluid was significantly higher than that in the malignant pleural effusion. 2) There was no difference between the activities of pleural fluid ADA in the tuberculous pleural effusion and that in the suspected tuberculous pleural effusion. 3) If the cut-off value of pleural fluid ADA activity is 40U/L, the sensitivity was 90.4% and the specificity was 86.3% in the dignosis the tuberculous pleural effusion. It is suggested that the measurement of ADA activity in pleural effusion is of value in the differential diagnosis of patients with tuberculous pleurisy from those with malignant pleurisy and others, particularly when the results of other laboratory and clinical tests are negative, if the value is over 40 U/L.

MEMS 및 센서 시스템 ; FET 형 이온센서용 무선원격측정시스템

김영진,손병기,이영철,정훈 한국센서학회 2001 센서학회지 Vol.10 No.3

A wireless telemetry measurement system, using ISFETs, was developed for environmental monitoring applications, industrial processes, medical treatments and so on. This system is composed of the measurement part located at remote site and a personal computer(PC) which control whole instrument process. The measurement part transmits measured data to the PC by RF transceiver, and transmitted data are analyzed and handled in the PC. Proposed system utilizing time division multiplexing for the transmission of 3channel ISFET signal. The measurement part has an identification number(ID) so that the PC controls many measurement parts separately. Experiments were performed using pH-ISFETs, and the implemented system operates well within designed specification and accuracy of 0.1 pH.

FET 형 용존 산소 센서의 유속에 의한 영향 조사와 감쇄 기법

김영진,손병기,이영철,정훈 한국센서학회 2001 센서학회지 Vol.10 No.3

Recently, FET type dissolved oxygen sensor was proposed to overcome the disadvantages of the amperometric Clark-type sensor. The inherent problem of the proposed sensor, however, is the flow dependence of the sensor perfomances since the proposed sensor detects the pH change in clue proximity to the working electrode. In this study, we decided the direction which minimize the flow effect in FIA(flow injection analysis) system. And a hydrodynamic buffer layer which can reduce the flow dependence were proposed. The suggested buffer-layers were formed onto sensing area and working electrode with mixed polymer matrix of TEOS(tetraethylorthosilicate) and DEDMS (diethoxydimethylsilane).

크롬 피콜린산 또는 카르니틴이 첨가된 사료급여가 비육돈의 성장 및 도체특성에 미치는 영향

김지훈,한인규,김대성,배극환,양종석,손현수,현영 한국영양사료학회 1999 韓國營養飼料學會誌 Vol.23 No.5

본 시험은 여러 가지 광물질과 혼합된 크롬 피콜린산이나 카르니틴을 첨가한 사료의 급여가 비육돈의 성장 및 도체특성에 미치는 영향을 조사하기 위하여 실시되었다. 평균체중 60.69±1.11 kg인 삼원교잡종 (Yorkshire×Landrace×Duroc) 비육돈 암수 180마리를 대조구, CFC{CrP + Fe₂O₃ + CaCO₃}, CFZC{CrP + Fe₂O₃ + ZnO + CaCO₃}, CZC-1 {CrP (1/300) + ZnO(9/300) + CaCO₃(290/300)}, CZC-2(CrP(1/300) + ZnO(14/300) + CaCO₃ (285/300)} 그리고 카르니틴구 등 6처리구에 성별로 배치하였다. 크롬 피콜린산이나 카르니틴에 의한 성장개선은 발견되지 않았다. 수퇘지에 있어서는 CFZC 처리구가 CZC-2 처리구보다 유의적으로 사료효율이 좋았다 (p<0.05). 도체특성에 있어서도 전체적으로 처리구간의 유의적인 차이는 발견되지 않았으나, 수퇘지에서는 CFZC 처리구가 대조구에 비해 등지방두께가 않았고 (p<0.05), 가장 좋은 도체등급은 CZC-2 처리구에서 나타났다. A study was conducted to investigate the effect of feeding diets containing chromium picolinate (CrP) or carnitine on growth and carcass characteristics in finishing pigs. A total of 180 cross bred pigs (Yorkshire x Landrace x Duroc; initial body weight of 60.7±1.1 kg) were assigned to 6 treatments : Control, CFC{CrP + Fe₂O₃ + CaCO₃}, CFZC{CrP + Fe₂O₃ + ZnO + CaCO₃}, CZC-1 {CrP (1/300) + ZnO(9/300) + CaCO₃(290/300)}, CZC-2(CrP(1/300) + ZnO(14/300) + CaCO₃ (285/300)} and camitine. No significant improvement was found in average daily gain (ADG), average daily feed intake (ADFI), and feed/gain ratio (F/G) by feeding diets containing chromium picolinate. The feed efficiency of male pigs fed with CFZC was better than pigs fed CZC-2 diet during the entire period (p<0.05). No differences were found in carcass characteristics between treatments expect that backfat thickness was reduced in male pigs fed the CFZC diet compared to the control (p<0.05). The best carcass grade was found in pigs fed CZC-2 the diet.

단조용 니켈기 초내열합금의 상태도 계산 및 검증

나영상,김인수,손성만,박노광 대한금속재료학회(대한금속학회) 1999 대한금속·재료학회지 Vol.37 No.9

Thermodynamic phase calculation and its experimental confirmation were made on a series of wrought Ni base superalloys. The Gibbs free energy of the constituent phases such as γ, γ', σ, etc can be expressed by applying the Sublattice model. Minimum Gibbs free energy condition was selected as the criteria for determining the equilibrium state. The calculated phase fraction and the transition temperature were experimentally validated by comparing the calculated value with the measured one. Isothermal aging treatment followed by electrochemical extraction and quantitative X-ray diffraction was employed for experimental validation of the calculated phase fraction, and the differential thermal analysis for the phase transition temperature. The calculated values for both phase fraction and transition temperature are well matched with the measured values. The calculated phase diagrams offer lots of useful information such as solution treatment temperature, TCP(Topologically Close Packed) phase formation, γ' phase fraction and driving force for precipitation, etc.