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      • KCI등재

        알러지 유발 단백질이 제거된 수용성 로열젤리의 피부미백 효과

        한상미,김정민,김세건,장혜리,여주홍,홍인표,우순옥 한국생약학회 2014 생약학회지 Vol.45 No.3

        Royal jelly composes of many components, especially protein. Protein is a major factor which cause allergy. We focused on water soluble royal jelly (WSRJ) that was removed allergy - inducing protein. 10-hyroxy-2-decenoic acid content of WSRJ is 2.42 g/100 g, which is double compared to that of lypophilized RJ. To further access WSRJ as a cosmetic ingredient and potential external treatment for topical use, we investigated its ability to inhibit tyrosinase activity and melanin biosynthesis on melanogenesis in B16F1 melanoma cells. We found that WSRJ increased the cell viability in B16F1 melanoma cell and WSRJ (1~10 mg/ml) inhibited melanin synthesis in with 10 nM a-melanocyte-stimulating hormone (a-MSH) for 48 h. WSRJ inhibited direct tyrosinase activity, which decreased melanin synthesis in a-MSH stimulated B16F1 melanoma cells. Thease findings suggest that WSRJ induces the down regulation of melanogenesis by inhibiting tyrosinase activation

      • KCI등재

        국산 프로폴리스의 Helicobacter pyrori 억제효과

        한상미,홍인표,우순옥,김세건,장혜리,장재선,Han, Sang Mi,Hong, In Phyo,Woo, Soon Ok,Kim, Se Gun,Jang, Hye Ri,Jang, Jae Seon 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.1

        Propolis possesses antimicrobial, anti-inflammatory, anaesthetic and immunostimulating activities. The aim of this study was to evaluated chemical composition and in vitro anti-Helicobacter pylori activity of eight propolis ethanolic extracts form different region in Korea. The chemical composition of eight Korean propolis was investicated by absorption spectrophotomety and UHPLC. Total penolic and total flavonoids content ranged between $21{\pm}1.5$ and $31{\pm}1.8$ gallic acid g/100 g and $7.2{\pm}0.9$ and $24.4{\pm}1.9$ quercetin g/100 g, respectively. The p-coumaric acid as standard components content ranged between $0.29{\pm}0.00$ and $2.34{\pm}0.02mg/g$ using UHPLC. Cinnamic acid content between $0.43{\pm}0.00$ and $1.33{\pm}0.01mg/g$. 80% ethanolic extract of propolis against H. pylori was evaluated by using the disc diffusion method. Ethanol was used as a control. Mean diameters of H. pylori growth inhibition using $10{\mu}g/mL$, $50{\mu}g/mL$ or $100{\mu}g/mL$ ethanol propolis per disc were 0, ranging from 0.9 to 2.1 mm and from 1.9 to 3.5 mm, respectively. In conclusion, variation in the chemical composition of Korean propolis were mainly due to the difference in the preferred regional plants visited by honeybees. Also Korean propolis possesses considerable antibacterial activity against H. pylori. There was not confirmed a correlation between the chemical composion contens and anti-H. pylori effect. The potential of propolis in the prevention or treatment of H. pylori infection is worth further extensive evaluation.

      • KCI등재

        정제봉독의 아나필락시스 쇼크 반응 연구

        한상미,홍인표,우순옥,김세건,장혜리,박관규,장영채 한국생약학회 2015 생약학회지 Vol.46 No.3

        This study was performed to examine the antigenic potential of purified bee venom (Apis mellifera L., PBV) collected using bee venom collector. Antigenic potential of PBV was examined by active systemic anaphylaxis (ASA) in guinea pigs. PBV was subcutaneously administered at 0.025 and 0.05 mg/kg and also as a suspension with adjuvant (Freund's complete adjuvant, FCA). Ovalbumin (OVA) as a suspension with adjuvant was used to introduce positive control response. In the weight measurement and clinical observation, experimental groups didn't show any significant changes compared with control group. In the autopsy of body, the abnormalities of lung were detected only in the positive control. In the ASA test, experimental groups didn't show any symptoms of anaphylaxis like piloerection, hyperpnea and staggering gait. These results suggested that PBV didn't have antigenic potential in guinea pig.

      • KCI등재

        채취 시기 및 지역에 따른 봉독의 성분 분석

        한상미,윤형주,백하주,Han, Sang-Mi,Yoon, Hyung-Joo,Baek, Ha-Ju 한국응용곤충학회 2012 한국응용곤충학회지 Vol.51 No.3

        서양종 꿀벌 독의 채집시기와 지역에 따른 봉독의 성분 변화 및 약리효과에 미치는 영향을 검토하였다. 채집시기는 5월부터 9월까지, 채집지역은 전국 35개 지역으로부터 채취한 봉독을 대상으로 2010년과 2011년 2년에 걸쳐 동일 지역에서 동일한 방법으로 봉독을 채취하였다. 채취한 봉독은 액체크로마토그래피를 통해 멜리틴과 아파민 그리고 포스포리파아제 A2의 성분 함량을 분석하였다. 그 결과 채집시기와 지역에 따른 성분에 유의한 차이는 확인되지 않았다(One way-ANOVA, Duncan's test (${\alpha}$=0.05)). 봉독의 성분은 채집시기와 지역에 관계없이 멜리틴 $55.2{\pm}2.07%$, 아파민 $2.57{\pm}0.103%$ 그리고 포스포리파아제 A2는 $12.51{\pm}0.37%$을 차지하였다. 이상의 결과로부터 봉독은 채취시기에 따른 주요 성분은 차이를 갖고 있지 않았으며, 이는 꿀벌의 먹이, 사육온도 등 외부 환경이 봉독 분비에 영향을 주지 않는 것으로 사료되었다. This study aims to investigate whether geographical variation affects the antibacterial component properties of honeybee (Apis mellifera L.) venom in Korea. Honeybee venom samples were collected from May to September, during 2010 and 2011, from 35 different sites, and were analyzed for major components, including melittin, apamin and phospholipase A2 were determined by a liquid chromatography using ammonium formate, acetonitrile, trifluoracetic acid. On average, melittin, apamin and phospholipase A2 were determined $55.2{\pm}2.07%$, $22.57{\pm}0.103%$, and $12.51{\pm}0.37%$, respectively. The ratio of the major components, including melittin, apamin and phospholipase A2 did not differ significantly according to flower or temperature during collections (One way-ANOVA, Duncan's test (${\alpha}$=0.05)).

      • KCI등재

        봉독의 피부자극시험 및 안점막자극시험

        한상미,이광길,여주홍 한국생약학회 2011 생약학회지 Vol.42 No.1

        The study of irritation and toxicity of honeybee(Apis mellifera. L) venom collected by a bee venom collector applied topically to the skin and mucous membrane were carried out to prove the safety of honeybee venom in clinical use. Animal for the research was the rabbit and the solution for the test was made from honeybee venom. Six animals were used fot the skin test and nine animals were used for the eye mucous membrane test. In results, both tests proved that honeybee venom makes no irritable reaction on skin and eye mucous membrane of rabbit. We consider that this result is helpful for saying about the safety of honeybee venom in clinical use.

      • KCI등재

        국내산 봉독의 여드름 유발균 및 피부 상재균 증식 억제 효과

        한상미,이광길,박관규,여주홍,김원태 한국생약학회 2009 생약학회지 Vol.40 No.3

        The in vitro antibacterial activities of honeybee(Apis mellifera. L) venom collected by a bee venom collector were investigated against several bacteria including antibiotic-susceptible and resistant Propionibacterium acnes. Honeybee venom was prepared with different concentrations and they showed strong antibacterial activites. Honeybee venom inhibited the growth of the tested antibiotic-resistant P. acnes at the concentration of 1 mg/ml. The inhibitory activities of the honeybee venom showed time-dependent manner. Honeybee venom did not influence the viability of human dermal fibroblast at the high concentration of less than 10 mg/ml. From these results, we expect that honeybee venom has strong antibacterial activities and has advantage for treating cure.

      • KCI등재

        베트남 학습자 대상 비즈니스 한국어 교육과정 개발을 위한 요구분석

        한상미,강승혜 한국언어문화교육학회 2020 언어와 문화 Vol.16 No.4

        This study aims to provide necessary data for developing a business Korean language curriculum through needs analysis for Korean language educators and Vietnamese Korean language learners. Business Korean is a crucial area of curriculum development for Vietnamese Korean learners, whose numbers have increased greatly since 2016, as a Korean for language occupational purposes in the area of KSP. A Google survey has been conducted on 83 Korean language educators(or teachers) in Korea and Vietnam and 193 Vietnamese Korean language learners studying Korean at institutions in Korea and Vietnam. Through the analysis, the following results were found.: First, Vietnamese Korean learners were hoping to get a job at Korean companies based in Korea and Vietnam. Their preferred jobs were in the order of translation, service, finance, and manufacturing industries. In particular, Korean learners in Vietnam responded that their favorite jobs were in the construction industry. Among the demands related to the curriculum framework, the preference for 12 weeks of study, three times a week, and 120 minutes per session were outstanding. Second, more than half of the Korean language educators said it is appropriate to start the Korean business curriculum at the intermediate level. There were high demands for 12 weeks of study, three times a week, and 90 minutes per session among them. Also, the analysis of responses to open questions showed that the Korean business curriculum should develop business vocabularies and expressions related to authenticity and practicality. .

      • KCI등재

        어린이집 교사의 교권인식 및 교권보장과 교권침해에 대한 실태 및 요구 조사

        한상미,김소향 미래유아교육학회 2020 미래유아교육학회지 Vol.27 No.4

        The purpose of this study was to provide basic data to improve teachers’ awareness of teachers’ rights at child care centers and to prepare measures to enhance teachers’ rights at child care centers by examining the awareness of teachers’ rights and the actual conditions of teachers’ rights and the need to establish teachers’ rights at child care centers. A total of 318 teachers from 47 child care centers in Busan were surveyed. The results were as follows: First, the meaning of teachers’ rights and the right to be guaranteed were recognized more than normal, but the level of awareness of laws and regulations related to teachers’ rights at child care centers and methods and organizations to protect their rights was low. Second, while the rights to education are kept above average, the actual conditions of guaranteeing rights to labor and rest, guaranteeing status and privacy, and guaranteeing rights to property were lower than normal. Third, regarding the current status of teachers’ infringement of teachers’ rights at child care centers, the violation of the right to educate, social and ethical authority, and professional authority was the highest, and the degree of violation of teachers’ rights was recognized as having been violated more than normal. Finally, efforts to improve professionalism were the highest among teachers’ efforts needed for the protection of teachers’ rights, while the most preceding task for the protection of teachers’ rights was the social atmosphere that respected teachers. In addition, preparing the necessary measures for the establishment of teaching authority was the highest. 본 연구는 어린이집 교사의 교권인식 및 교권보장과 교권침해에 대한 인식과 실태 및 요구를 살펴봄으로써, 어린이집 교사의 교권에 대한 올바른 인식 개선을 도모하며 교권 신장방안을 마련하는데 기초자료를 제공하는 것이 목적이다. 연구대상은 부산지역 47개소 어린이집 교사 총 318명이다. 연구 결과는 다음과 같다. 첫째, 교권의 의미와 교권을 보장 받아야 할 권리에 대해 보통 이상으로 인식하고 있었으나, 어린이집 교사의 권리와 관련된 법규에 대한 인식과 권리를 지키기 위한 방법과 단체에 대한 인식 수준은 낮은 것으로 나타났다. 둘째, 교육에 대한 권리는 보통이상으로 지켜지고 있는 반면, 노동 및 휴식에 대한 권리보장, 신분⋅사생활 보장, 정당한 보상 받을 권리보장 실태는 보통이하로 낮게 나타났다. 셋째, 어린이집 교사의 교권침해 실태에 대해서는 교육할 권리와 사회⋅윤리적 권위 및 전문적 권위의 무시 및 간섭에 대한 침해가 가장 높게 나타났으며, 교권침해에 대하여 보통이상으로 침해 받고 있다고 인식하였다. 마지막으로, 교권보호를 위해 필요한 교사의 노력으로는 전문성 향상을 위한 노력이 가장 높게 나타났고, 교권보호를 위해 가장 선행되어야 할 과제는 교사를 존경하는 사회분위기가 가장 높게 나타났다. 또한 교권 확립을 위해 필요한 대책은 교권 확립을 위한 제도 마련이 가장 높게 나타났다.

      • KCI등재

        창업교육 기반 카페 동아리 활동이 지적장애 고등학생의 진로결정 효능감 및 직업준비도와 비장애학생의 장애학생에 대한 사회적 거리감에 미친 영향

        한상미,김유리 이화여자대학교 특수교육연구소 2019 특수교육 Vol.18 No.4

        Purpose: The purpose of this study was to investigate the impact of the entrepreneurship education-based cafe club on career decision self-efficacy and vocational readiness of students with intellectual disabilities, and on social distance of peers to students with intellectual disabilities. Method: Twenty high school students with intellectual disabilities(10 students of an experimental group and 10 students of a control group) and 14 peers without intellectual disabilities participated in this study. Ten students with intellectual disabilities and 14 peers participated in nine classroom sessions(100 minutes per session), in which they were instructed on new information and skills related to the cafe management. Also, the students were provided four sessions(60 minutes per session) to practice the skills in a cafe environment. Results: Students with intellectual disabilities in the experimental group had significantly greater increase in career decision efficacy and vocational readiness compared to the control group. Statistically significant differences were found in pretest-posttest changes of social distance of peers without intellectual disabilities to students with intellectual disabilities. Conclusion: The entrepreneurship education can be one of the approaches regarding career education programs for students with intellectual disabilities in school settings. Additionally, this study had practical implications in that it provided a guideline for developing and applying the systematic club in which high school students with and without intellectual disabilities could participate. 연구목적: 본 연구는 창업교육 기반 카페 동아리 활동을 통해 지적장애 고등학생의 진로결정 효능감 및 직업준비도, 비장애학생의 장애학생에 대한 사회적 거리감에 미치는 영향을 알아보고자 하였다. 연구방법: 지적장애 고등학생 20명(실험집단 10명, 통제집단 10명)과 비장애학생 14명(실험집단)이 참여하였다. 창업교육 기반 카페 동아리 활동은 교실 수업 9회기(1회당 100분)와 학교카페 현장실습 4회기(1회기당 60분)에 걸쳐 실시되었다. 본 연구는 지적장애 학생의 진로결정 효능감 및 직업준비도 변화 정도를 알아보기 위해 사전-사후 통제집단 설계를 하였으며 비장애학생의 지적장애 학생에 대한 사회적 거리감은 사전-사후 단일집단 설계를 사용하였다. 연구결과: 실험집단 지적장애 학생의 진로결정 효능감과 직업준비도는 통제집단 학생과 비교하여 통계적으로 유의미하게 향상되었다. 또한 비장애학생의 장애학생에 대한 사회적 거리감도 카페 동아리 활동 참여 후 통계적으로 유의한 차이를 나타내어 비장애 학생의 사회적 거리감을 감소시키는데 긍정적 영향을 미쳤음을 알 수 있었다. 결론: 본 연구는 창업교육이 지적장애 학생을 위한 직업교육의 하나의 대안이 될 수 있음을 보여주었다. 특히 장애학생과 비장애 학생이 함께 참여하는 동아리 활동 개발 및 적용에 대한 기초 자료를 제공하였다는 점에서 의의를 지닌다.

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