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      • 질산칼슘 처리농도에 따른 수축견사의 이화학적 특성

        이광길,이용우,박영환,남중희 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.1

        This study was carried out in order to find out the relationship between physical and chemical properties of silk fiber treated by concentrated calcium nitrate solution. The tensile, thermal and dynamic mechanical properties are also examined on Ca(NO3) 2, treated silk fibers. The tensile properties of silk fibers treated by calcium nitrate changed with a concentration. The thermal behavior were also affected by the concentration of calcium nitrate. The degradation temperature(endotherms) and glass transition temperature shifted to lower temperature as the treated concentration increased. It is thought that the physical properties are strongly related to the structure and morphology of Ca(NO3)2, treated silk fibers. As a result, these give property changes with a concentration dependence.

      • KCI등재

        Seasonal festival 이 가지는 集團治療的 意義

        李光吉 大韓神經精神醫學會 1974 신경정신의학 Vol.13 No.4

        I analyzed the observations, recordings and psychological data derived from the participation to the seasonal festival in national mental hospital as a therapist, with the result as follows. 1. Enjoyment of play pleasurably. 2. Experience of pain-pleasure principle especially in preparing festival, that is rehearsal. 3. Elevation of sense of reality and objectivity in mutual activity and cooperation. 4. Self-realization through contest and cooperation. 5. Fastening of solidarity linking common identity with aim for freeing from psychic disturbance. 6. Perceiving sense of thankfulness and humour sense.

      • 곤충유래 키틴/키토산의 구조특성

        이광길,이용우,권해용,여주홍,박인규,남진,설광열 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.2

        The chitin was isolated from various kinds of insects such as exuvia of Psacothea hilaris Pascoe, silkworm pupa, Agrius convolvuli or from cuticle of cockroach by treatment with dilute HCl and NaOH. The chemical and crystalline structure was characterized by FT-lR and X-ray diffractometer. All of the chitins extracted from insects showed characteristic α-chitin peaks at the Brags angle 2θ=9.3°, 19.4°, and 23.5° by X-ray diffraction analysis. The transition from chitin to chitosan was confirmed by IR spectra and the degree of deacetylation of the crab shell, silkworm pupa, cockroach, and Psacothea hilaris Pascoe was 70.9, 76.4, 75.5, and 74.1% , respectively. The double diffraction peaks of insect chitosan were observed at 2θ =10° and 20, indicating the characteristic of hydrated crystalline structure of chitosan.

      • 질산칼슘 처리 농도에 따른 수출견사의 구조특성

        이광길,이용우,박영환,남종희 한국잠사학회 1997 한국잠사곤충학회지 Vol.39 No.2

        The IR crystallinity index of Calcium nitrate treated silk fiber decreased proportionally to the concentration of calcium nitrate. A partial change of conformation was observed in the concentration of over 46.4~47.6% changing from ß-sheet or to random coil in the crystalline region. This is in coincidence with the result of crystallinity index, which was started to be reduced in the concentration range of46.4~47.6%. A same trend was observed for the X-ray order factor, birefringence, degree of orientation and surface structure. These structural parameters were remarkably changed on the treatment of silk fibers with concentration of 46.4%~47.6% calcium nitrate. Therefore, it seems that there exists a critical concentration of calcium nitrate in affecting the structure and morphology of silk fibers. Acrording to the examination of surface morphology, the fine stripe was observed in the direction of fiber axis at 46.4% concentration. However, the treated concentration was exceeded by 47.6%, the cracks were appeared severely on the fiber surface in the transverse direction as well as fiber axis direction. This result might be related to the tensile properties, specially a tenacity of silk fibers. As a result of quantitative analysis analysis of a dilute acid hydrolysis, three diffirent regions, which are known as a amorphous, semi-crystalline and crystalline region, could be obsteained. The hydrolysis rate curves were different with various concentrations of treatment and the relative contents of each region could be calculated.

      • 한국인 악성임파종의 조직학적 및 면역조직학적 연구

        이광길,양우익,이유복 연세대학교 대학원 1985 延世論叢 Vol.21 No.5

        Abstract Various designations uses in malignant lymphomas lacked precise definition and many investigators had uses different terms for the came tumor. As a result of this imprecise terminology, no meaningful clinicopathologic correlations could be eatablished. Rappaport classification was based purely on the cellular morphology and growth pattern of the lymphomas. It was widely accepted, not only because it was relatively easy to apply, but also because it was clinically useful in the design of treatment protocols and in predicting response to therapy and survival of patients. However, based on the new knowledge regarding the nature and origin of the lymphoid cells in malignant lymptomas, many investigators attempted to correct the scientific inaccuracies of the Rappaport classification by proposing new classifications. Lukes and Collins, as well as Lennert et al., placed great emphasis on the fact that the non-Hodgkin's lymphomas were tutors of B- or T-lymphocytes, and they asserted that immunologic identification of lymphoid ceils is an integral part of the diagnosis and classification of the lymphomas. The existence of many classifications not only has resulted in confusion and controversy, but also has made it impossible to compare effectively the results of clinical studios utilizing different systems. To resolve this, the National Cancer Institute of U.S.A. has suggested a new Working Formulation(WF) for Clinical Usage. Relatively recently immunologic methods suck as immunofluorescent and immunoperoxidase staining were popularly used in the diagnosis and stuffy of lymptoproliferative diseases. We performed this stuffy to reclassify the malignant lymphomas according to WF baaed on the histologic features and immunologic phenotypes and to compare the usability and advantages of various immunohistologic staining methods. The material consisted of 53 crises of malignant lymphomas examined at the Department of Pathology, Yonsei University College of Medicine from May, 1983 through December, 1984. All crises were subjected to histopathological analysis, review of clinical records and immunologic studies. The paraffin blocks were sectioned at 5 micron thickness and sections were stained with hematoxylin-eosin and if needed be, methyl-green pyronin. Immunologic studies were also performed on the sections of paraffin-embedded tissue and on 9 cases of frozen sections; immunoperoxidase staining for demonstration of cytoplasmic immunoglobulins and lysozyme and immunofluorescent staining with fluorescein-conjugated monoclonal antibody for demonstration of HLA-DR and Leu-1 antigens in the tumor cells. Prepared elides were examined by light microscope or immunofluorescent miscroscope as occasion demands. The results were as follows: 1) Of the 53 crises of malignant lymphomas, 28 cases(52.9%) and 15 cases(28.2%) were belong to intermediate and high grades respectively. Most of these were "diffuse, small cleaved", "diffuse, mixed", "diffuse, large cell", and "large cell, immunoblastic" types, which ranged from 15.1% to 18.9% respectively. True histiocytic lymphoma was absent. 2) According to Rappaport classification, histiocytic lymphomaa were the most frequent type (30.2%) and the next, lymphocytic, poorly differentiated type (18.9%). 3) Among the 44 crises of morphologically putative B-cell lymphomas, immunoper-oxidase staining for immunoglobulin was positive in 68.2% of crises and immunofluorescent staining for anti-HLA-DR, positive in 65.9%. 4) Monoclonality of light chain was observes in 66.7% of the cases that stained positive for immunoglobulin by immunoperoxidase method. 5) Histiocytes were present and mixed with tumor cells ranging from mild to severe degree in 41.5% of the 44 crises of morphologically putative B-cell lymphomas. In mycosis fungoides histiocytes were mixed with tumor cello in 80%, but in lymphoblastic lymphomas, all cases lacked histiocytes.

      • 중성염에 의한 견사의 수축작용

        이광길,이용우,님중희 한국잠사학회 1997 한국잠사곤충학회지 Vol.39 No.1

        This study was carried out to find out the relationship between qualities and contraction phenomenon of silk fibers by treatment of concentrated neutral salts. The contraction effects of silk fibers showed the critical point on the treatment conditions of concentration, temperature and time, among three kinds of neutral salts such as calcium nitrate, calcium chloride and lithium bromide. But, The silk fibers, pretreated with bromide and/or formaldehyde, did not show the contraction upon treating with calcium nitrate. This indicates that tyrosine and serine can be correlated with the contraction reaction because of coupling these amino acids with bromide and formaldehyde. In conclusion, a mechanism for the contraction of silk fiber with highly concentrated calcium nitrate solution is supposed as follows. At the initial stage of reaction, the water was penetrated into the amorphous regions and fibers swollen, therefore, the contraction took place mainly in amorphous regions, which have plenty of functional groups with hydroxyl residues. Then, as the calcium nitrate is penetrated into the microfibril, the hydrogen bonds of tyrosine and serine residues are broken and crystalline regions are more and more influenced by increasing concentration of calcium nitrate solution. Microfibrils of crystalline regions become entangled, contracted to linear direction and rearranged to form new stable hydrogen bonds.

      • KCI등재후보

        원통형 탈황설비 정비용 비계 연구개발

        이광길,김우곤,엄석호,강철민,강정기,오철석,서주식,김경재,박종인,장몽룡,김무성,김대년,정수호 대한안전경영과학회 2020 대한안전경영과학회지 Vol.22 No.3

        The desulfurizers facility is cylindrical shape. To operate properly it needs nozzles cleaned, get rid of lime adhesion and sludge, repair the wear and corrosion of facility regularly. For this purpose, workers shall access the ceiling or vertical wall at high place. Ordinary scaffoldings such as steel pipe scaffolding or system scaffolding have been using so that workers can access them. With these ordinary scaffoldings, openings around cylindrical wall are inevitable which make workers can expose always to the risk of falling. The purpose of this study is to develop customized scaffolding to minimize the openings to prevent workers form falling during maintenance it. It consists of a hexagonal central tower and six trapezoidal outer towers. And the bracing among the towers have connected each other for self-standing and for maintaining the structure of towers. Span decks, the circumference footstools, steps, etc. are laid on each floor. The safety is reviewed by structural analysis and performance test. With this study, openings each floor of this scaffold are removed. The gap between the cylindrical wall and the edge of the work stage is approximately 100 mm. Therefore, we expect that workers can work safely and efficiently.

      • 간세포암종의 세침흡인 세포학적 소견 - 247예에 대한 연구 -

        이광길,이종태,최수임,박찬일,Lee, Kwang-Gil,Lee, Jong-Tae,Choi, Soo-Im,Park, Chan-Il 대한세포병리학회 1990 대한세포병리학회지 Vol.1 No.1

        Hepatocellular carcinoma (HCC) is malignant tumor frequently occurring in Koreans. There have been few reports regarding the cytologic findings of fine needle aspiration (FNA) of HCC. Most have suggested a diagnostic problem in the cytology distinguishing HCC from some benign hepatic lesions-for example, a regeneration nodule in cirrhosis and liver cell adenoma. In spite of its high frequency in Korea, no cytologic study has been reported, concerning the FNA of HCC. In an attempt to achieve cytologic criteria for the diagnosis of HCC, the authors studied retrospectively cytopathologic findings of 247 cases of HCC. These cases were confirmed either by histoiogic examination including lobectomy, biopsy, or ceil block material, or, when tissue diagnosis was unavailable, by a high serum alpha-fetoprotein level (over 400 I. U.). All aspiration smears were stained by the Papanicolaou method. In each case, the smears were analyzed for cell patterns and various cytomorphology of the tumor cells. The smear background was assessed for the presence of tumor cell necrosis and inflammatory components and compared to that of metastatic carcinomas. The cell patterns were classified as trabecular, acinar, dispersed, and irregular. The cytologic parameters analyzed included the degree of nuclear atypia and the presence of mitoses, intranuclear cytoplasmic inclusions, nucleolar prominency, endothelial lining, multinucleated giant cells, eosinophiic globules, bile, and Mallory body. Most of the FNA of HCC showed markedly cellular smears. The tumor cells were most frequently arranged in a trabecular pattern (80.3%). The irregular (12.6%), the acinar (5.5%), and the dispersed patterns (1.7%) followed in decreasing frequency. Individual hepatoma cells were larger than normal liver cells. However, they had morphologic features characteristic of the hepatic cells the cells were round or polygonal, their cytoplasm was abundant and granular with eosinophilic or amphophilic stainability, and their nuclei were round to oval, located centrally, and tended to have prominent nucleoli. Anaplasia and pleomorphism of tumor cells were generally mild to moderate. These findings existed even in very well differentiated cases. Mitotic figures were present in about 85% of the cases. Prominent nucleoli were observed only in about half the cases. The frequency of other cytologic features was as follows intranuclear cytoplasmic inclusion in 86.8% : endothelial lining in 56.1% : bile in 19.8% : and giant cells in 60.1%. Clear cells were often present in 11.7%, Most aspiration smears of HCC displayed clean background without necrosis or inflammatory material in contrast to the dirty, necrotic background of metastatic cancers and cholangiocarcinomas. Based on the above mentioned features, it is suqqested that the cytologic critieria most important for the diagnosis of HCC include a markedly cellular smear, trabecular pattern, hepatocytoid appearance of tumor cells, endothelial lining, the presence of bile, giant cells, intranuclear cytoplasmic inclusions, and prominent nucleoli, Among these, trabecular pattern, endothelial lining, giant cells and clean smear background are points to be considered in differentiating HCC from metastatic and cholangiocellular carcinoma.

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