용매에 따른 팽이버섯(Flammulina velutipes) 추출물의 생리활성 효과

이우행(Woo-Haeng Lee),한소라(So-Ra Han),유상철(Sang-Cheol Yu),오태진(Tae-Jin Oh) 한국식품영양과학회 2018 한국식품영양과학회지 Vol.47 No.1

본 연구에서는 식용버섯으로 사용되는 팽이버섯을 아세톤, 에틸아세테이트 및 에탄올로 추출하여 팽이버섯 각각의 용매 추출물에 따른 항산화 및 항균 활성을 확인하였다. 항산화 활성은 총폴리페놀 함량, 총플라보노이드 함량, DPPH 라디칼 소거능 및 ABTS 라디칼 소거능 등을 이용하여 조사하였으며, 또한 한천배지확산법을 이용하여 6종 균주에 대한 항균 활성을 탐색하였다. 팽이버섯의 총폴리페놀 함량을 측정한 결과에서는 에틸아세테이트 추출물이 다른 용매 추출물보다 유의적으로 높게 조사되었으며, 에틸아세테이트 추출물> 에탄올 추출물> 아세톤 추출물 순으로 나타났다. 총플라보노이드 함량에서도 에틸아세테이트 추출물이 다른 용매 추출물보다 유의적으로 높은 결과를 보였다. DPPH와 ABTS 라디칼 소거능은 에틸아세테이트, 아세톤 및 에탄올 추출물 모두 항산화제로 잘 알려진 ascorbic acid보다 높은 소거능을 보였다. DPPH 라디칼 소거능을 측정한 결과에서는 에탄올 추출물이 다른 용매들보다 높은 소거능을 보였으며, ABTS 라디칼 소거능의 경우에는 아세톤과 에탄올 추출물이 유의적으로 높은 항산화 활성을 나타내었다. 팽이버섯의 다제내성 균주에 대한 항균 활성은 에탄올 추출물의 Ent. cloacae에 대한 활성을 제외하고, 3종의 용매 추출물 모두에서 B. subtilis, S. aureus, M. luteus, E. coli, P. aeruginosa 및 Ent. cloacae 등 6종의 모든 균주에 대하여 항균활성을 나타냈다. 특히 에틸아세테이트 추출물의 경우에는 E. coli와 M. luteus 등에서 가장 높은 항균 활성을 보였으며, B. subtilis와 S. aureus에 대해서는 아세톤 추출물에서 가장 강한 생육저해환을 확인할 수 있었다. 항산화 성분인 총폴리페놀 함량 및 총플라보노이드 함량과 항산화 활성인 DPPH 및 ABTS 라디칼 소거능 사이에는 음의 상관관계를 보였으며, 폴리페놀 및 플라보노이드 함량이 팽이버섯의 항산화 활성에 주된 기여를 하지 않는 것으로 나타났다. 또한, 다양한 균주에 대한 팽이버섯의 항균 활성에 플라보노이드 함량이 강한 양의 상관관계를 보여 플라보노이드 화합물이 팽이버섯의 항균 활성에 기여하는 화합물인 것으로 생각한다. 지금까지 본 연구에서 확인한 결과처럼 팽이버섯 추출물의 항산화 및 다양한 균주에 대한 항균성 등 관련 기능성을 확인함으로써 결과적으로 팽이버섯은 천연 기능성 소재로써 활용 가능성이 매우 높다고 할 수 있다. The aim of this study was to investigate the in vitro antioxidant and antimicrobial activities of acetone, ethanol, and ethyl acetate extracts from Flammulina velutipes. Antioxidant activity was evaluated based on total contents of polyphenols and flavonoids, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity. Total contents of polyphenols and flavonoids were the highest in ethyl acetate extracts. The DPPH radical scavenging activities of these extracts were in the order of ethanol (82.3%)> ethyl acetate (79.6%)> acetone (78.4%)> ascorbic acid (76.8%). Ethanol extract showed significantly different (P<0.05) DPPH radical scavenging activities compared with other extracts. ABTS radical scavenging effects were 74.7% and 73.0% for acetone and ethanol extracts, respectively. Antimicrobial activities of F. velutipes extracts were evaluated by the paper disk diffusion method against six strains of microbes, including Bacillus subtilis, Staphylococcus aureus, Micrococcus luteus, Escherichia coli, Pseudomonas aeruginosa, and Enterobacter cloacae. Acetone and ethyl acetate extracts showed antimicrobial effects against all tested strains, and especially ethyl acetate extract had strong antimicrobial activities against M. luteus and E. coli. Antioxidant activities were negatively correlated with contents of total polyphenols (−0.192 with DPPH and −0.953 with ABTS) and total flavonoids (−0.717 with DPPH and −0.704 with ABTS). A positive relationship was established among total flavonoid contents and DPPH and ABTS radical scavenging activities. This study confirms positive antioxidant and antimicrobial activities for F. velutipes.

미성숙 목화다래 메탄올 추출물의 항산화 효능 평가

박희정,이기영 한국자원식물학회 2013 한국자원식물학회지 Vol.26 No.4

본 연구에서는 미성숙 목화다래 추출물의 항산화능을 알아보기 위해 다래를 크기별로 구분하여 총 폴리페놀 및 플라보노이드 함량과 DPPH radical의 소거능, 아질산염 소거능, ABTS radical 소거능 및 SOD 유사활성을 측정하였다. 추출물의 페놀성 화합물과 플라보노이드 함량은 목화다래의 크기가 작을수록 높게 나타났으며, DPPH radical 소거능 및 ABTS radical의 소거능 또한 작은 크기에서 상대적으로 높은 활성을 보였다. 이는 다른 선행연구들에서와 같이 페놀함량이 높은 실험군에서 DPPH radical 소거능과ABTS radical 소거능이 높게 나타나 유의성을 확인하였다. 아질산염 소거능은 인체 위 내부환경과 같은 pH 1.2에서는높은 활성을 보이다가 pH 4.2에서는 점차로 활성이 떨어지는 결과를 보였으며, 중성에 가까운 pH 6.0에서는 아질산염소거에 영향을 미치지 못하는 것으로 확인되었다. 또한 SOD유사활성은 대조군인 ascorbic acid에 비하면 낮은 활성이었지만, 기 보고된 다른 천연물들의 효소활성과 비교하였을 때 더 높거나 비슷한 결과를 나타냈다. 이와 같이 본 실험을 통해 미성숙 목화다래의 뛰어난 항산화 활성을 확인할수 있었으며, 이는 향후 화장품이나 기능성 식품 등에 있어서 새로운 소재개발의 가능성을 시사해주는 것으로서, 그동안 주로 천연 솜의 생산을 위해 재배되어왔던 목화의 제한적 활용성을 더욱 다양하게 해줄 것으로 기대된다. The results of the content of total polyphenol and flavonoid, DPPH (1-1-diphenyl-2-picryl-hydrazyl) radicalscavenging activity, ABTS [2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)] radical scavenging activity, nitritescavenging activity and SOD-like activity of methanol extracts from immature cotton boll are follows. The contents of totalpolyphenol and flavonoid compound were higher in small size cotton boll, and DPPH and ABTS radical scavenging activityalso showed a relatively high activity in the small size. These results indicate that there is a correlation between phenolcontent and DPPH radical scavenging, ABTS radical scavenging. The test concentrations of immature cotton boll extract formeasuring DPPH and ABTS radical scavenging activities were set as 1.25, 2.5, 5, 10 and 20 mg/ml. Immature cotton bollhas high radical scavenging activity at the concentration of 1.25~20 mg/ml and the result showed a tendency to increase ina concentration-dependent. The nitrite scavenging activity showed high activity in the pH 1.2, and the result in the pH 4.2showed progressively less active, and in the pH 6.0 near neutral was confirmed that does not affect the nitrite scavenging. Inaddition, SOD-like activity showed somewhat lower activity compared with ascorbic acid, but tended to be higher whencompared with the results of the other natural substances. Through this experiment, we confirmed that immature cotton bollwas excellent antioxidant activity. Therefore, it is demonstrated that the cotton suggest the possibility of development ofnew material for cosmetic product or functional food in the future, and is expected to make a greater usability.

DPPH 방법을 통한 토사자, 보골지(補骨脂), 사상자(蛇床子), 음양곽(淫羊藿)의 항산화 활성에 대한 연구

오명숙,김도림,강지웅,김산웅,유태원,박정열,김동민,박완수,장문석,박수연,박성규,Oh, Myung-Sook,Kim, Do-Rim,Kang, Ji-Ung,Kim, San-Woong,Yu, Tae-Weon,Park, Jung-Yeul,Kim, Dong-Min,Park, Wan-Su,Chang, Mun-Seog,Park, Soo-Yeon,Park, Seong-Ky 대한한의학방제학회 2005 大韓韓醫學方劑學會誌 Vol.13 No.2

The present study was conducted to compare antioxidant activity of Cuscutae Semen, Psoraleae Fructus, Cnidii Fructus and Epimedii Herba by DPPH radical scavenging activity. The extract was studied using diphenyl-picryl-hydrazyl (DPPH) for DPPH method. DPPH radical scavenging activity was measured after. .10, 20 and 30 minutes. The extract was tested by 1, 5, 10, 50, 100, 500 and $1000{\mu}g/ml$ concentrations. The results showed that the extract scavenged DPPH radical with time-dependent manner. Also, the extract showed dose-dependent DPPH radical scavenging activity. The extract of Cuscutae Semen, Psoraleae Fructus, Cnidii Fructus and Epimedii Herba scavenged DPPH radical with the IC50 being 2.7, 3.2, 2.9 and 1.1 mg/ml, respectively. In conclusion, the extract of Epimedii Herba, Cuscutae Semen, Cnidii Fructus and Psoraleae Fructus have antioxidant activity for the treatment of male sterility.

DPPH 방법을 통한 녹차의 항산화 활성에 대한 연구

강정숙 제주대학교 방사능이용연구소 2003 연구보고 Vol.17 No.-

The present study was conducted to compare antioxidant activity of green tea and related other common teas according to growing, processing and brewing by examining radical scavenging activity using DPPH (2,2 Diphenyl 1-picryl hydrazyl). SC_(50) of epigallocatechin gallate (EGCG) for 0.1mM DPPH radical were 5.5μM or 4.2㎎/ℓ by weight, then catechin, 14μM or 2.5㎎/ℓand vit C, 22μM or 3.9㎎/ℓ respectively. Okro tea powder of 24.2㎎/ℓ or green tea powder of 25.2㎎/ℓ was used to reach SC_(50) for 0.1mM DPPH. One serving of 2g green tea provides antioxidant activity equivalent to 109∼147㎎ EGCG, 130∼182㎎ catechin or 142∼168㎎ vit C. Teas from the first spring flush had the highest radical scavenging activity when compared with later harvest green tea grown in the same region, but there virtually is no difference by the harvest time. Chinese green tea, Younjung had the highest antioxidant activity among other green teas tested providing antioxidant capacity equivalent to 168㎎ EGCG or 188㎎ vit C per 2g serving, but partially fermented Chinese teas had much lower antioxidant activity than any green tea tested. Black tea which is fully fermented showed as strong antioxidant activity as green teas when compared in teabag. One teabag of green teas from market provided antixidant effect equivalent to 50∼85㎎ EGCG, 70∼105㎎ catechin or 60∼95㎎ vit C. Teas made of persimon, pine needle, mulberry had comparatively low antioxidant effect of 2.5∼3.5㎎ EGCG or 15∼20㎎ vit C per teabag. The third brewed green gea still had enough antioxidant activity, while tea from teabag brewed for 3min or 5min did not have any difference in their antioxidant activity. In conclusion, reen tea from the first harvest had more antioxidant activity and okro which is grown under shade had more or at least as much antioxidant activity as the green tea grown in the same region. Partially fermented Chinese teas were low in antioxidant effect while fully fermented black tea had strong antioxidant ctivity. More systemic studies are needed to clarify the changes in tea catechins in relation with fermantation process.

Assessment of Antioxidative Capacity in Relation to Seed Traits of Rice Varieties

Hong-Keun Song,Joung-kuk Ahn,Kwang-Ho Kim,Sun-Joo Lee,Jin-Yeong Baek,Ill-Min Chung 韓國作物學會 2006 Korean journal of crop science Vol.51 No.6

In order to assess antioxidant capacity in relation to seed traits of rice (Oryza sativa L.), ninety-six varieties were examined for antioxidative activity of brown rice grain using superoxide dismutase (SOD), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and thiobarbituric acid (TBA) assays. Overall, average total activities measured by the three methods were of very wide range between 64% and 13%. Significant differences were noted depending on the variety and evaluation method. Rice varieties with foreign origin, middle maturity, colored hulls, and colorless awn exhibited statistically significant higher total activity. As for the measurements, total activity was significantly correlated with SOD (r=0.29***), DPPH (r=0.80***) and TBA (r=0.76***). Between the three activities, SOD was not positively correlated with DPPH (r=0.15*), while TBA was significantly correlated with DPPH value (r=0.51***). DPPH (55.20%) and TBA (50.36%) were significantly higher in foreign rice, while SOD activity (44.29%) was significantly higher in domestic rice. However, an average total activity was significantly higher in foreign rice (47.31%) than in domestic rice (35.92%). SOD, DPPH and TBA activities of middle maturity in maturity time were the highest total activity (44.96%) and significantly differed from the other two groups. Total activity was significantly higher in rice with a colorless awn (42.18%) than with a colored awn (35.87%).

약용 식물 발효액(들빛)의 항산화, Angiotensin Converting Enzyme 저해 및 Nitric Oxide 생성 조절 효과

조은경,최영주,갈상완 한국응용생명화학회 2010 Journal of Applied Biological Chemistry (J. Appl. Vol.53 No.2

This study was aimed to investigate the contents of flavonoids and the biological activity of fermented beverage of medical plants, DeulBit (DB). 50 g of Cassia semen (Cassia tora L.), 50 g of Omija (Schisandra chinensis Baillon.), 50 g of Gugija (Lycium chinense Mill), 50g of Menthae herba, 75 g of Chrysanthemum indicum Linne, 25 g of Dioscorea batatas, 5 g of Lindera obtusiloba Blume, 150 g of Polygonatum odoratum, 25 g of Glycyrrhiza uralensis, 25 g of Acanthopanacis cortex, 100 g of green tea (Camellia sinensis), and 100 g of Laminaria japonica was fermented with sucrose (50.0~60.0˚Brix.) and 0.5% of deep sea water in 10 L of distilled water for six months at room temperature. Total flavonoids contents of DB was calculated to 3.4±0.5 µg/g and antioxidative activity of DB was measured by using DPPH radical scavenging and SOD-like activity. DPPH radical scavenging and SOD-like activity of DB was 96% and 29% at 100% of DB, respectively. In addition, DB indicated about 88% and 66% of the xanthine oxidase and angiotensin converting enzyme inhibitory activities at 1% and 10% of DB, respectively and showed fibrinolytic activity. Nitric oxide (NO) synthesis was increased to 15 times by addition of DB. In addition, NO productions of the macrophages RAW264.7 cells stimulated with lipopolysaccharide (LPS) were reduced to 40.4% by addition of DB. These results suggested that DB is significant role for antioxidative and fibrinolytic activity, and have the strong xanthine oxidase and angiotensin converting enzyme inhibitory activities. 본 연구에서는 결명자, 오미자, 구기자, 박하, 국화, 산마, 생강, 둥글레, 감초, 오가피, 녹차, 다시마를 발효시킨 들빛 발효액으로 여러 가지 생리활성에 대하여 분석하였다. 우선, 들빛 발효액의 플라보노이드 함량과 DPPH 활성을 측정하였다. 그결과 들빛 발효액의 총 플라보이드 함량은 3.4±0.5 mg/g으로, DPPH법을 통해 측정한 들빛 발효액의 radical 소거능은 96%로 나타났다. 들빛 발효액의 SOD 유사활성은 29%로 나타내었고, XOase에 의해 생성된 superoxide radical 소거능은 88%로 높게 나타났다. 들빛 발효액의 항고혈압 측정실험에서는 66%의 저해률을 나타내어 ACE 저해 활성이 뛰어난 것으로 나타났다. 혈전분해능에 대한 들빛 발효액의 분석 결과는 혈전용해제로 알려져 있는 plasmin과 유사한 활성을 보이고 있다. 들빛 발효액과 면역활성과의 연관성은 NO 생성 증가율과 LPS에 의해 유도되는 NO 생성 억제율 분석으로 조사되었다. 그 결과, 들빛 발효액은 무려 15배의 높은 NO 생성 증가율을 보였다. 한 LPS에 의해 유도된 NO 생성 억제율은 들빛 발효액 100%에서 42%로 나타나 높은 면역조절능을 나타내었다. 이상의 결과는 들빛 발효액의 우수한 생리활성을 증명하고 있고, 항산화력, 항고혈압 효과, 혈전분해능 및 면역 조절활성이 높은 것으로 나타나 기능성 음료의 소재로서 그 활용도가 높을 것으로판단된다.

천연화장품의 재료로서 복숭아 잎 추출물의 기능성 평가

장혜륜,권혜진,박두현 한국피부과학연구원 2014 대한피부미용학회지 Vol.12 No.2

Goal of this study is to evaluate the suitability of peach leaf as a raw material for natural cosmetics basedon specific functions that are antioxidative activity, antibacterial activity, and tyrosinase inhibition. Theantioxidative activity was determined based on DPPH-scavenging effect and the antibacterial activity wasmeasured based on inhibition effect of bacterial community isolated from face skin of 4 women. The peachleaf was grinded using ball meal for improving extraction efficiency. Triply distilled (highly pure) water or99.9% ethanol was used as a solvent for extraction of functional compounds from ground peach leaf. Dryweight of the extract was not quantitatively determined but functional efficiency of the extract was evaluatedbased on ratio of the peach leaf weight to the solvent volume. The ratio of peach leaf weight to solventvolume was adjusted to 20 g to 1,000 mL, which was employed as a criterion to determine functional activityof the extract. Ten times-diluted peach leaf extract was employed to determine the DPPH-scavengingactivity and the tyrosinase inhibition activity; however, the peach leaf extract was not diluted to determineantibacterial activity. Functional activity was the mean value of results obtained from triply repeated tests. The DPPH-scavenging activity of the water-extracted and the ethanol-extracted peach leaf was approximate57.5% and 68.0%, respectively; meanwhile, the tyrosinase inhibition activity of the water-extracted and theethanol-extracted peach leaf was approximate 52.5% and 41.9%, respectively. Differences between thewater-extracted and the ethanol-extracted peach leaf for antioxidative, tyrosinase inhibition, and antibacterialactivity was approximate 15%, 20%, and 10%, respectively. Conclusively, the functional activity contained inthe peach leaf is considered to be suitable as a raw material for the natural cosmetics.

식물유래 천연색소의 항산화 활성

부희옥,황성진,배춘식,박수현,송원섭 한국자원식물학회 2011 한국자원식물학회지 Vol.24 No.1

The objective of this study was to determine the effect of antioxidant enzyme activity and radical scavenging activities of thirteen kinds of natural plant pigments. The analytic method of antioxidant activities were measured by estimating DPPH free radical scavenging and nitrite scavenging ability activity. The free radical scavenging activity by method using stable free radical DPPH was the highest in the red cabbaged pigment. Addition of ethanol extract 1㎎/㎖ from onion peel pigment displayed remarkable effect on nitrite scavenging ability about 91.9%. Antioxidative enzyme activity was evaluated in terms of superoxide dismutase(SOD), catalase(CAT), and ascorbate peroxidase(APX) activity. The bitter melon pigment had the highest SOD activity of 87.3%. The activities of CAT and APX were higher in the mulberry leave pigment compared with other natural plant pigments. In contrast, CAT activity of plant pigment samples were unaffected. These results suggest that natural plant pigment had the potent biological activities such as antioxidant enzyme activities, and that their activities exhibited differently depending on each kind of pigments. 선발된 13종의 천연색소 추출물에 대하여 free radical 소거활성 및 아질산염 소거활성, 그리고 항산화효소 활성에 대하여 조사하였다. 천연색소 종류별 free radical 소거활성을 보면 적양배추(red cabbage), 양파껍질(onion peel), 레드비트(redbeet), 흑미(black rice), 오디(mulberry) 색소 등에서 상대적으로 높게 나타났고, 청치자(blue gardenia) 및 황치자(yellow gardenia), 완숙여주(mature bitter melon) 색소 등에서는 비교적 낮은 활성을 보였다 또한 아질산염에 대한 소거능을 조사한 결과, 반응용액 pH 1.2의 경우 양파껍질, 레드비트, 치자황, 지황(chinese foxglove), 치자청 색소에서 높은 소거 활성을 나타냈으며, 적양배추와 오디 색소에서는 상대적으로 낮은 소거활성을 보였다. 그리고 반응용액 pH 6.0에서는 대부분의 색소 추출물에서 아질산염 소거활성을 나타내지 않았다. 그리고 항산화효소 활성 결과를 보면, SOD 활성의 경우 여주, 흑미, 포도과피 등에서 높은 활성을 나타냈고, 자색고구마, 지황에서도 비교적 높은 활성을 보였다. 또한 APX 활성은 뽕잎, 오디, 지황의 순으로 높은 활성을 보였으며, CAT 활성도 APX 결과와 비슷한 양상으로 나타났다. 이는 천연색소에 있어 항산화 효소의 종류에 따라 그 활성에 차이가 있음을 알 수 있다. 본 연구결과를 통해 다양한 생리활성을 지니고 있는 천연식물색소에 대해 높은 항산화 기능성을 확인함으로서 향후 고부가가치의 향장소재 및 식품소재로서의 개발가능성이 매우 높음을 시사해 주고 있다.

한방천연물 및 약용버섯을 첨가한 조미간장소스의 항산화 및 항균활성

정엽,송효남,박소영,박시현,염은선,정우용,김수민 한국식품영양학회 2023 韓國食品營養學會誌 Vol.36 No.1

To increase antioxidant and antibacterial activities of seasoned soy sauce, five kinds of oriental medicinal plant(Scutellaria baicalensis (P1), Coptis japonica makino (P2), Citriunshius pericarpium (P3), Zizyphi spinosi semen (P4), Crataegus pinnatifida (P5)) and four kinds of medicinal mushrooms(Inonotus obliquus (M1), Hericium erinaceus (M2), Phellinus linteus (M3), Lentinula edodes (M4)) were added to seasoned soy sauce. Soluble solid content, pH, salinity, total polyphenol & flavonoid contents were determined. DPPH & ABTS radical scavenging activities, SOD-like activity, and antibacterial activity were analyzed. Experimental sauces showed decreased pH but significant increases of soluble solid content and salinity. Total polyphenol content was 12.76 μg GAE/g in the control. However, M1 and P1 sauce had significantly higher polyphenol contents at 352.14 and 528.25 μg GAE/g, respectively. Total flavonoids content also showed the same pattern. DPPH free radical scavenging activity was the lowest in the control at 15.75%. It was the highest at 81.80% in M1 and 68.88% in P1. ABTS free radical scavenging activity and SOD-like activity showed the same tendencies. They were higher in the experimental groups than in the control. As for the antibacterial activity analyzed by the paper-disc method, the activity increased the most in P1 and P2. In particular, P2 had the strongest antibacterial activity. Its activity against different microorganisms was in the order of Staphylococcus aureus > Bacillus cereus > Escherichia coli > Salmonella typhimurium. In conclusion, these new sauces show increased antioxidative and antioxidant activities. Therefore, they are expected to be used in various ways as a functional soy sauce.

창이자의 항산화 활성 및 항균효과

신선우,이정호,방극수 한국자원식물학회 2012 한국자원식물학회지 Vol.25 No.4

Xanthium sibiricum using extracts antioxidant activity and antimicrobial activity were analyzed and the following results were obtained. Antioxidant activity using DPPH as a VLC column fractionation experiments XS-5 (water fraction of Xanthium sibiricum) concentration in the 500 ㎍/ml to 87.53 percent in the highest antioxidant activity was found, VLC fractionation in XS-3-2 (eluted with chloroform/methanol 7~10%) concentration of 500 ㎍/ml, showed the antioxidant activity of 78.72%. The antioxidant activity measured by PCL XS-1 (n-hexane fraction of Xanthium sibiricum) XS-5 (water fraction of Xanthium sibiricum) and 13.32 nmol open column XS-3-2 (eluted with chloroform/methanol 7~10%) showed antioxidant activity with 14.34 nmol. The antimicrobial activity against Candida albicans XS-2 (methlene chloride fraction fo Xanthium sibiricum) is the concentration of 500 ㎍/disc 1 mm of the clean zone was formed. XS-3 (ethanol fraction fo Xanthium sibiricum) in the antioxidant activity using DPPH and PCL had higher antibacterial activity was low. 창이자 추출물을 이용하여 항산화활성 및 항균활성을 측정한 결과 다음과 같은 결론을 얻었다. DPPH를 이용한 항산화 활성 실험결과 VLC column로 분획한 XS-5에서 처리농도 500 ㎍/ml에서의 87.53%로 가장 우수한 항산화 활성이 나타났으며, VLC로 분획한 XS-3-2의 처리농도 500 ㎍/ml에서 78.72%의 항산화 활성이 나타났다. PCL법으로 측정한 항산화 활성은 XS-1에서 13.32 nmol과 open column XS-3-2에서 14.34 nmol으로 항산화력을 나타났다. Candidaalbicans에 대한 항균활성은 XS-2의 처리농도가 500 ㎍/disc에서는 1 mm의 clean zone이 형성되었다. XS-3에서DPPH와 PCL를 이용한 항산화 활성은 높았지만 항균활성은낮게 나타났다. 창이자 추출물은 항산화 활성과 항균 활성은상호 상관관계가 없는 것으로 판단된다.