Effect of biostimulation and bioaugmentation on hydrocarbon degradation and detoxification of diesel-contaminated soil: a microcosm study

Patricia Giovanella,Lídia de Azevedo Duarte,Daniela Mayumi Kita,Valéria Maia de Oliveira,Lara Durães Sette 한국미생물학회 2021 The journal of microbiology Vol.59 No.7

Soil contamination with diesel oil is quite common duringprocesses of transport and storage. Bioremediation is considereda safe, economical, and environmentally friendly approachfor contaminated soil treatment. In this context, studiesusing hydrocarbon bioremediation have focused on totalpetroleum hydrocarbon (TPH) analysis to assess process effectiveness,while ecotoxicity has been neglected. Thus, thisstudy aimed to select a microbial consortium capable of detoxifyingdiesel oil and apply this consortium to the bioremediationof soil contaminated with this environmental pollutantthrough different bioremediation approaches. Gas chromatography(GC-FID) was used to analyze diesel oil degradation,while ecotoxicological bioassays with the bioindicatorsArtemia sp., Aliivibrio fischeri (Microtox), and Cucumissativus were used to assess detoxification. After 90 days ofbioremediation, we found that the biostimulation and biostimulation/bioaugmentation approaches showed higher ratesof diesel oil degradation in relation to natural attenuation(41.9 and 26.7%, respectively). Phytotoxicity increased in thebiostimulation and biostimulation/bioaugmentation treatmentsduring the degradation process, whereas in the Microtoxtest, the toxicity was the same in these treatments as thatin the natural attenuation treatment. In both the phytotoxicityand Microtox tests, bioaugmentation treatment showed lowertoxicity. However, compared with natural attenuation, thisapproach did not show satisfactory hydrocarbon degradation. Based on the microcosm experiments results, we concludethat a broader analysis of the success of bioremediation requiresthe performance of toxicity bioassays.

Core-shell structured quorum quenching beads for more sustainable anti-biofouling in membrane bioreactors

Yu, Huarong,Lee, Kibaek,Zhang, Xiaolei,Choo, Kwang-Ho Elsevier 2019 Water research Vol.150 No.-

<P><B>Abstract</B></P> <P>Efficient media designs for microbial quorum quenching (QQ) are essential to enable maximal biofouling control in membrane bioreactors (MBRs). Here we introduce a novel, double-layered, biocarrier design, which has QQ bacteria in the shell layer with biostimulating agents in the core, for effective membrane biofouling control. Confining the biostimulant within dense polymer materials permits its controlled release over an extended period. The provision of the biostimulant from the core to the outer shell, where the QQ bacteria are encapsulated, facilitates their prolonged survival and active life. The core-shell structured QQ bead with the stimulant inside, which inhibits biofilm formation, shows the best fouling mitigation in laboratory testing of MBRs, while enhancing signal molecule degradation and lowering exopolymer secretion. This new, layered QQ bead, which has dual functions of bioaugmentation and biostimulation, supports a highly efficient and sustainable anti-biofouling strategy.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A new, core-shell structured quorum quenching (QQ) bacteria carrier is investigated. </LI> <LI> An outer layer encapsulates QQ bacteria, while a dense core contains a biostimulant. </LI> <LI> Controlled release of the biostimulant prolongs the activity and survival of the QQ bacteria. </LI> <LI> This layered structure enables highly efficient and sustainable biofouling control. </LI> <LI> The QQ medium demonstrates the combined effects of bioaugmention and biostimulation. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Heavy metal and sulfate removal from sulfate-rich synthetic mine drainages using sulfate reducing bacteria

Hwang, Sun Kyung,Jho, Eun Hea Elsevier 2018 Science of the Total Environment Vol.635 No.-

<P><B>Abstract</B></P> <P>The removals of heavy metals and sulfate in the synthetic acid mine drainages (AMDs) by <I>Desulfovibrio desulfuricans</I>, sulfate-reducing bacteria (SRB), and the indigenous bacteria isolated from the mine area soil sample were studied to compare the AMD treatment efficiencies. The AMD treatment by the <I>D. desulfuricans</I> grown in the <I>Desulfovibrio</I> medium was used to represent bioaugmentation, while the AMD treatment by the indigenous bacteria grown in the <I>Desulfovibrio</I> medium was used to represent biostimulation. The consumption of lactate and sulfate suggested that the zinc (Zn) removal in the Zn-spiked <I>Desulfovibrio</I> medium by <I>D. desulfuricans</I> involved chemical precipitation and biosorption. The complete Zn removal by <I>D. desulfuricans</I> took 24 h, while the indigenous bacteria took 360 h. The significantly lower rate can probably be attributed to the composition of the culture. The removal of Zn in the sulfate-rich synthetic AMD-containing <I>Desulfovibrio</I> medium (i.e., AMD) was adversely affected by the presence of other heavy metals. Also, the sulfate reduction by <I>D. desulfuricans</I> and the indigenous bacteria was reduced from 47% to 20% and from 36% to 6%, respectively. The inhibitive effects on the removal of heavy metals and sulfate were greater with the Zn/Cu-spiked AMD than the Zn-spiked AMD. Overall, the indigenous bacteria showed potential for removing heavy metals and sulfate in AMDs, while the removal efficiency was lower than <I>D. desulfuricans</I>. The continuous supply of carbon sources with an adaptation period may be required to enhance the AMD treatment efficiency by the indigenous bacteria.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>D. desulfuricans</I> removed heavy metals and sulfate in synthetic AMDs. </LI> <LI> Sulfate reducing activity of <I>D. desulfuricans</I> was promoted by heavy metals. </LI> <LI> Indigenous bacteria isolated from a mine area exhibited sulfate reducing capacity. </LI> <LI> Heavy metal and sulfate in the synthetic AMD could be removed by the indigenous culture. </LI> <LI> Biostimulation of indigenous bacteria may be potentially used to treat AMD in situ. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Natural attenuation, biostimulation 및 Rhodococcus sp. EH831을 이용한 bioaugmentation에 의한 디젤 오염 토양의 정화

이은희 ( Eun Hee Lee ),강연실 ( Yeon Sil Kang ),조경숙 ( Kyung Suk Cho ) 한국미생물생명공학회(구 한국산업미생물학회) 2011 한국미생물·생명공학회지 Vol.39 No.1

3가지 종류의 생물정화법인 natural attenuation (NA), biostimulation (BS) 및 bioaugmentation (BA) 방법을 디젤로 오염된 토양을 정화하기 위해 적용하여, 각 방법에 의한 정화효율과 미생물 활성을 계면활성제 첨가 유무(Tween80)에 따라 비교하였다. 토양 정화 초기 단계에서는 Rhodococcus sp. EH831을 접종원으로 이용하는 BA 방법에 의한 토양 정화효율이 가장 좋았다. 3가지 생물정화방법 모두에서 계면활성제 첨가는 토양 정화효율에 영향을 미치지 않았다. 토양의 탈수소활성(DHA)과 잔류 총석유계탄화수소(TPHs) 농도는 음의 상관관계를 보였다: DHA (μg-TPF·g-dry soil-1 d-1) = -0.02 × TPHs concentration (mg-TPHs·kg-dry soil-1) + 425.76 (2500≤ TPHs concentration≤20000, p < 0.01). Three bioremediation methods, natural attenuation (NA), biostimulation (BS) and bioaugmentation (BA) were applied to remediate diesel-contaminated soil, with their remediation efficiencies and soil microbial activities compared both with and without surfactant (Tween 80). BA treatment employing Rhodococcus sp. EH831 was the most effective for the remediation of diesel-contaminated soil at initial remediation stage. On the addition of surfactant, no significant effect on the remediation performance was observed. A negative correlation was found between the dehydrogenase activity (DHA) and residual concentration of total petroleum hydrocarbons (TPHs) at below 20,000 mg-TPHs ·kg-dry soil-1, as follows: DHA (μg-TPF(Triphenylformazan) kg-dry soil-1 d-1) = -0.02 × TPHs concentration (mg-TPHs·kg-dry soil-1) + 425.76 (2500 ≤ TPHs concentration ≤ 20000, p < 0.01).

저출력 레이저의 생자극효과를 이용한 조직재생에 관한 최신연구동향

강경리 ( Kyung Lhi Kang ),박준봉 ( Joon Bong Park ),지유진 ( Yu Jin Jee ),류동목 ( Donk Mok Ryu ),이덕원 ( Deok Won Lee ) 한국조직공학과 재생의학회 2009 조직공학과 재생의학 Vol.6 No.4

Nowadays laser gets attention again as a novel method in various treatment fields, such as oral surgery, endodontic and periodontal treatment, etc. Also biostimulation is an special area that we can`t pass over. Biostimulation means that low-level laser irradiation promotes the regeneration process in bone, soft tissue, and peri-implant tissue. Although the mechanism has not been elucidated, many reports have supported the biostimulation effect and it is generally accepted. In this feature article, researches up to now were reviewed to know the trends and future research focus.

염소화 에텐 오염 지하수의 생물활성 복원이 생태계 교란에 미치는 영향

홍진경 ( Jin-kyung Hong ),김수빈 ( Soo Bin Kim ),이태권 ( Tae Kwon Lee ) 한국물환경학회 2024 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2024 No.-

Non-contact Tactile Sensation by Pulsed Laser Stimulation

( Ji Sun Kim ),( Han Byeol Oh ),( A Hee Kim ),( Bong Jun Goh ),( Jun Sik Kim ),( Eun Suk Lee ),( Soon Cheol Chung ),( Jong Rak Park ),( Jae Hoon Jun ) 한국감성과학회 2015 추계학술대회 Vol.2015 No.-

With the development of optical techniques, there is a growing interest in laser applications in biological science and medicine. Laser has the merit that it can be quantitatively delivered to the small area with the desired energy, frequency and pulse width. This merit was utilized to non-contact tactile sensation using laser-induced mechanical effects. Various laser parameters were applied to investigate the effect of these parameters on laser stimulation for tactile sensation. This study showed the possibility of non-contact tactile sensation using laser. The results can be used in laser-induced biostimulation and tactile sensation without tissue damage.

Biodegradation of Crude Oil-contaminated Soil using Canned-food-industry Wastewater Sludge for Soil Application

Efsun Dindar,F. Olcay Topaç Şağban,Hüseyin S. Başkaya 대한토목학회 2017 KSCE JOURNAL OF CIVIL ENGINEERING Vol.21 No.5

The objective of this study was to evaluate the effects of crude oil (application doses of 0.5% and 5%) from hydrocarbon contamination on the removal of the Total Petroleum Hydrocarbons (TPH) from soil and to determine the removal of TPH at different temperatures (18°C and 28°C) during an incubation period of 240 days. The possible use of wastewater sludge as a biostimulating agent in crude oil-contaminated soils was also evaluated. The results of the 240 days of incubation indicated that the TPH removal percentages in crude oil-contaminated and sludge-treated soils at 18°C were 89% and 79%, for doses of 0.5 and 5%, respectively. Incubation at 28°C resulted in higher TPH removal with removal percentages of 83% (dose of 0.5%) and 91% (dose of 5%). The degradation of crude oil in contaminated soil treated with a 5% dose was significantly enhanced by the addition of wastewater sludge, whereas no apparent biostimulating effect on TPH removal was observed in the case of low-dose (0.5%) crude oil contamination.

Transcriptomic Insights into Abies koreana Drought Tolerance Conferred by Aureobasidium pullulans AK10

Park Jungwook,Mohamed Mannaa,Gil Han,Jung Hyejung,Jeon Hyo Seong,김진철,Park Ae Ran,서영수 한국식물병리학회 2024 Plant Pathology Journal Vol.40 No.1

The conservation of the endangered Korean fir, Abies koreana, is of critical ecological importance. In our previous study, a yeast-like fungus identified as Aureobasidium pullulans AK10, was isolated and shown to enhance drought tolerance in A. koreana seedlings. In this study, the effectiveness of Au. pullulans AK10 treatment in enhancing drought tolerance in A. koreana was confirmed. Furthermore, using transcriptome analysis, we compared A. koreana seedlings treated with Au. pullulans AK10 to untreated controls under drought conditions to elucidate the molecular responses involved in increased drought tolerance. Our findings revealed a predominance of downregulated genes in the treated seedlings, suggesting a strategic reallocation of resources to enhance stress defense. Further exploration of enriched Kyoto Encyclopedia of Genes and Genomes pathways and protein-protein interaction networks revealed significant alterations in functional systems known to fortify drought tolerance, including the terpenoid backbone biosynthesis, calcium signaling pathway, pyruvate metabolism, brassinosteroid biosynthesis, and, crucially, flavonoid biosynthesis, renowned for enhancing plant drought resistance. These findings deepen our comprehension of how AK10 biostimulation enhances the resilience of A. koreana to drought stress, marking a substantial advancement in the effort to conserve this endangered tree species through environmentally sustainable treatment.

Current concepts of Laser dentistry

은희종(Hee-jong Eun) 대한치과의사협회 2011 대한치과의사협회지 Vol.49 No.11

Various sorts of dental devices for the dental treatment have been introduced and adopted during past several years. Dental Laser, among all devices, seems to be possible for applying to over the whole treatments and widely employed. Furthermore, this article is to introduce that Dental Laser is intended to care patients secured with the effectual treatment against Conventional procedures based upon its studies. The advantages of Dental Laser treatment-along with LLLT (Low Level Laser Therapy) effect, biostimulation effect, and minimal invasive technique - make the patients reduce their fear of the operation, lighten the inconvenience of post operation, and shorten the treatment period. In particular, it is worth considering that the use of laserassisted therapies is associated with a marked reduction in the use of analgesics and anti-inflammatory medications compared with conventional procedures. This article is to state advantages and differences of Dental Laser treatment compared with Conventional procedures , and to emphasize to become well-acquainted with the precautions for safety and effective Dental Laser treatment. In case of operating Dental Laser with lack of the instructions, it will cause the unpredicted fatal results; therefore this treatment requires special care in its operation. Henceforward, it is anticipated that infinite treatment protocols will be introduced by applying Dental Laser, and this is to address the utilization of Dental Laser.