Scutellaria Extract Decreases the Proportion of Side Population Cells in a Myeloma Cell Line by Down-regulating the Expression of ABCG2 Protein

Lin, Mei-Gui,Liu, Li-Ping,Li, Chen-Yin,Zhang, Meng,Chen, Yuling,Qin, Jian,Gu, Yue-Yu,Li, Zhi,Wu, Xin-Lin,Mo, Sui-Lin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Background and Aims: Scutellaria is one of the most popular traditional Chinese herbal remedies against various human diseases, including cancer. In this study, we examined the active effects of Scutellaria extract and its main flavonoid constituents on the proportion of side population cells within human multiple myeloma cell line RPMI8226 in vitro and explored the potential molecular mechanisms involved. Materials and Methods: The contents of flavonoids in ethanolic extract of Scutellaria baicalensis Georgi were determined using high performance liquid chromatography. The antiproliferative effect of the ethanolic extract on RPMI-8226 was determined by CCK assay. Apoptosis was measured by annexin combining with propidium iodide in a flow cytometer. Cell cycle analysis was performed by propidium iodide staining in combination with flow cytometry analysis. Hoechst 33342 exclusion assay was used for the identification of side population within RPMI8226 cells. The expression of ABCG2 protein was assessed by Western blotting assay. Results: The content of major flavonoids constitutents of Scutellaria extract was baicalin (10.2%), wogonoside (2.50%), baicalein (2.29%), and wogonin (0.99%), respectively. The crude Scutellaria extract did not show significant anti-proliferative effect, apoptosis induction and cell cycle arrest in RPMI-8226 within the concentrations of $1-75{\mu}g/mL$. However, the ethanolic extract, baicalein, wogonin and baicalin reduced the side population cells in RPMI-8226, and data showed that baicalein and wogonin had stronger inhibitory effects. Correspondingly, they also exhibited significant effects on decreasing the expression level of ABCG2 protein in RPMI-8226 in vitro. Conclusions: Our results for the first time demonstrated a novel mechanism of action for Scutellaria extract and its main active flavonoids, namely targeting SP cells by modulating the expression of ABCG2 protein. This study provides an insight for new therapeutic strategies targeting cancer stem cells of multiple myeloma.

Angelica sinensis Supercritical Fluid CO2 Extract Attenuates D-Galactose-Induced Liver and Kidney Impairment in Mice by Suppressing Oxidative Stress and Inflammation

Zhi-Zhun Mo,Zhi-Xiu Lin,ZiRen Su,Lin Zheng,Hui-Lin Li,JianHui Xie,Yan-Fang Xian,Tie-Gang Yi,Shui-Qing Huang,Jian-Ping Chen 한국식품영양과학회 2018 Journal of medicinal food Vol.21 No.9

Angelica sinensis (AS, Danggui in Chinese) is an important herbal component of various traditional formulae for the management of asthenia and its tonic effects. Although AS has been shown to ameliorate cognitive damage and nerve toxicity in D-galactose (D-gal)-elicited senescent mice brain, its effects on liver and kidney injury have not yet been explored. In this work, mice were subjected to hypodermic injection with D-gal (200 mg/kg) and orally gavaged with AS (20, 40, or 80 mg/kg) once a day for 8 successive weeks. Results revealed that AS significantly improved liver and kidney function as assessed by organ index and functional parameters. In addition, AS pretreatment effectively ameliorated the histological deterioration. AS attenuated the MDA level and markedly enhanced the activities and gene expressions of antioxidative enzymes, namely Cu, Zn-SOD, CAT, and GPx. Furthermore, AS markedly inhibited the D-gal-mediated increment of expressions of inflammatory cytokines iNOS, COX-2, IκBα, p-IκBα, and p65 and promoted the IκBα expression level in both hepatic and renal tissues. In sum, AS pretreatment could effectively guard the liver and kidney of mice from D-gal-induced injury, and the underlying mechanism was deemed to be intimately related to attenuating oxidative response and inflammatory stress.

Synthesis and Evaluation O-Benzyl Oxime-ether Derivatives Containing β-Methoxyacrylate Moiety for Insecticidal and Fungicidal Activities

Zhi-Bin Hu,He-An Luo,Xiao-Guang Wang,Ming-Zhi Huang,Lu Huang,Huai-Lin Pang,Chun-Hui Mao,Hui Pei,Chao-Qun Huang,Jiong Sun,Ping-Le Liu,Ai-Ping Liu 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.4

In attempt to lead compounds exhibiting both insecticidal and fungicidal activities, a series of O-benzyl oximeether derivatives were designed and synthesized by introducing β-methoxyacrylate pharmacophore into a scaffold. The insecticidal activity against Aphis fabae and the fungicidal activity against Erysiphe graminis were screened. The title compounds exhibited remarkable insecticidal and fungicidal activities. The most potent compound 6d was identified. Its insecticidal LC50 against A. fabae is 6.4 mg/L, which is lower than that of chlorfenapyr (19.4 mg/L) and even close to the level of imidacloprid (4.8 mg/L). Its fungicidal EC90 in preventive and curative treatment against E. graminis are 2.2 and 4.8 mg/L, respectively, which are lower than azoxystrobin (7.0 and 5.9 mg/L). These results indicate that compound 6d can be considered as a lead for further developing new O-benzyl oxime-ether typed candidates with both fungicidal and insecticidal activities.

Efficient hole injection in blue organic light-emitting devices by using a double hole injection layer to improve chromaticity and electrical characteristics

Hua-Ping Lin,Fan Zhou,Xiao-Wen Zhang,Dong-Bin Yu,Jun Li,Liang Zhang,Xue-Yin Jiang,Zhi-Lin Zhang 한국물리학회 2011 Current Applied Physics Vol.11 No.3

A double hole injection layer consisting of Ag_2O//MoO_x was applied to a 4,40-bis (2,2-diphenylvinyl)-1,10-biphenyl-based blue organic light-emitting device (OLED). The hole current injection of the devicewas improved and the performance of the blue OLED was enhanced. We have observed that the insertion of such double layer leads to a striking improvement in chromaticity and electrical characteristics. This device showed much better chromaticity and less current-induced color shift as compared to the corresponding single hole injection layer counterparts. Using this strategy, a striking improvement in the electrical properties with lower driving voltage, higher power efficiency and a weak current-induced fluorescence quenching was achieved. This could be explained by the fact that an interlayer of Ag_2O//MoO_x provided a stepped energy level which greatly facilitated hole injection and hence enhanced injection current. The result is verified by using the J―V curves of ‘only’ devices and further explained with the help of Fowler-Nordheim (F-N) tunneling theory.

Synthesis and Evaluation O-Benzyl Oxime-ether Derivatives Containing β-Methoxyacrylate Moiety for Insecticidal and Fungicidal Activities

Hu, Zhi-Bin,Luo, He-An,Wang, Xiao-Guang,Huang, Ming-Zhi,Huang, Lu,Pang, Huai-Lin,Mao, Chun-Hui,Pei, Hui,Huang, Chao-Qun,Sun, Jiong,Liu, Ping-Le,Liu, Ai-Ping Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.4

In attempt to lead compounds exhibiting both insecticidal and fungicidal activities, a series of O-benzyl oximeether derivatives were designed and synthesized by introducing ${\beta}$-methoxyacrylate pharmacophore into a scaffold. The insecticidal activity against Aphis fabae and the fungicidal activity against Erysiphe graminis were screened. The title compounds exhibited remarkable insecticidal and fungicidal activities. The most potent compound 6d was identified. Its insecticidal $LC_{50}$ against A. fabae is 6.4 mg/L, which is lower than that of chlorfenapyr (19.4 mg/L) and even close to the level of imidacloprid (4.8 mg/L). Its fungicidal $EC_{90}$ in preventive and curative treatment against E. graminis are 2.2 and 4.8 mg/L, respectively, which are lower than azoxystrobin (7.0 and 5.9 mg/L). These results indicate that compound 6d can be considered as a lead for further developing new O-benzyl oxime-ether typed candidates with both fungicidal and insecticidal activities.

A high performance of BPhen-based white organic light-emitting devices with a dual-emitting layer and its electroluminescent spectral property

Hua-Ping Lin,Fan Zhou,Jun Li,Xiao-Wen Zhang,Dong-Bin Yu,Liang Zhang,Xue-Yin Jiang,Zhi-Lin Zhang 한국공업화학회 2011 Journal of Industrial and Engineering Chemistry Vol.17 No.4

This work demonstrated the fabrication of white organic light-emitting devices (WOLEDs) using a dual emitting layer (d-EML) consisting of blue and ‘white’ emitters. In this d-EML system, the blue emitter not only emits but also assists the incomplete energy transfer. More importantly, it behaves as an effective trapping site for holes, which contributes to the efficient recombination of electron–hole pairs. The d-EML was constructed between the hole-transmitting layer (HTL) and the electron-transmitting layer (ETL) of Alq3 and BPhen. The thickness of the blue emitter used in the d-EML devices has an important effect on chromaticity and efficiency. Through the optimization of device structure, the reasonable white emission with Commission Internationale de L’Eclairage (CIE) color coordinates of (0.33, 0.33) and little color shift was obtained. The device showed an applicable luminance with its maximum luminance of 22,874 cd/m2 at a driving voltage of 16 V. The maximum luminance efficiency was achieved 8.10 cd/A,and the maximum power efficiency was reached 5.07 Lm/W. The result is explained with the help of the excitons generation and diffusion theory. According to the theory of excitons generation and diffusion,an equation has been set up which concerns electroluminescent spectra to the thickness of the two emitters and to the exciton diffusion length.

Alternaria yunnanensis sp. nov., a New Alternaria Species Causing Foliage Spot of Rubber Tree in China

( Zhi-ying Cai ),( Yi-xian Liu ),( Yu-ping Shi ),( Li-ming Dai ),( Lan-lan Li ),( Hong-jun Mu ),( Mei-lin Lv ),( Xiao-yong Liu ) 한국균학회 2019 Mycobiology Vol.47 No.1

A new species of Alternaria causing leaf spots on the rubber tree (Hevea brasiliensis) in Yunnan, China, was isolated, examined, and illustrated. Morphologically, it belongs to the section Porri of Alternaria, which produces relatively large conidia and a simple or branched, filamentous long beak. It is, however, characterized by conidiophores gradually enlarging near the apex into a clavate conidiogenous cell and long ellipsoid to obclavate, smoothwalled conidia with a long filamentous beak. Molecular phylogenetic analyses based on ITS rDNA, GAPDH, and TEF1-alpha sequences demonstrate that the phytopathogen falls in the clade of the section Porri, being most closely related to A. sidae, A. sennae, A. deseriticola, A. cyamopsidis, A. rostellata, A. nitrimali, A. crassa, and A. thunbergiae.

Molecular cloning of two novel stearoyl-acyl desaturase genes from winterness wheat

Zhi-Fu Guo,Feng-Zhen Li,Xiao-Gang Ma,Feng Lin,Hui Ma,Li-Jing Chen,Ming Zhong,Li-Ping Bai,Ying Yi 한국유전학회 2011 Genes & Genomics Vol.33 No.5

Using RT-PCR and rapid amplification of cDNA ends, two new full-length cDNAs of SAD (TaSAD1 and TaSAD2) were obtained from a hardiest winter wheat cultivar (Mironovskaya808). Sequence comparison analysis showed that the deduced amino acid sequences of TaSAD1 and TaSAD2 had high similarity to those of other reported SAD proteins. They were also different each other by some substitutions, insertions and/or deletions involving single amino acid residues or motifs. Based on evolution analysis, it was clear that all SAD genes from Poaceae were closer than those from other genus such as Arabidopsis,Glycine, Triadica, Brassica, Sesamum and Bassia. All SAD genes clustered into two major groups in Poaceae. Meanwhile,TaSAD1 and TaSAD2 were clustered into different groups. The tertiary structure prediction indicated that both TaSAD1 and TaSAD2 proteins were a compact globular protein and their model structures almost were the same.

Attenuation of Experimental Autoimmune Hepatitis in Mice with Bone Mesenchymal Stem Cell-Derived Exosomes Carrying MicroRNA-223-3p

Yong-Ping Chen,Feng-Bin Lu,Da-Zhi Chen,Lu Chen,En-De Hu,Jin-Lu Wu,Hui Li,Yue-Wen Gong,Zhuo Lin,Xiao-Dong Wang,Ji Li,Xiao-Ya Jin,Lan-Man Xu 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.12

MicroRNA-223-3p (miR-223-3p) is one of the potential microRNAs that have been shown to alleviate inflammatory responses in pre-clinical investigations and is highly encased in exosomes derived from bone mesenchymal stem cells (MSC-exosomes). MSC-exosomes are able to function as carriers to deliver microRNAs into cells. Autoimmune hepatitis is one of the challenging liver diseases with no effective treatment other than steroid hormones. Here, we examined whether MSC-exosomes can transfer miR-223-3p to treat autoimmune hepatitis in an experimental model. We found that MSC-exosomes were successfully incorporated with miR-223-3p and delivered miR-223-3p into macrophages. Moreover, there was no toxic effect of exosomes on the macrophages. Furthermore, treatments of either exosomes or exosomes with miR-223-3p successfully attenuated inflammatory responses in the liver of autoimmune hepatitis and inflammatory cytokine release in both the liver and macrophages. The mechanism may be related to the regulation of miR-223-3p level and STAT3 expression in the liver and macrophages. These results suggest that MSC-exosomes can be used to deliver miR-223-3p for the treatment of autoimmune hepatitis.

Controlled Synthesis of Branched Polystyrene via RAFT Technique in the Presence of Chain Transfer Monomer p-Vinyl Benzene Sulfonyl Chloride

Cui-Ping Li,Jia-Qiang Wang,Yan Shi,Zan Liu,Jun Lin,Zhi-Feng Fu 한국고분자학회 2012 Macromolecular Research Vol.20 No.8

Branched polystyrene was first obtained via a reversible addition-fragmentation chain transfer (RAFT)process in the presence of chain transfer monomer p-vinyl benzene sulfonyl chloride (VBSC) in benzene at 60 oC with 2-(ethoxycarbonyl)prop-2-yl dithiobenzoate as the RAFT agent and 2,2-azobisisobutyronitrile as an initiator. During the RAFT polymerization, VBSC played the role of branching agent. It could not only copolymerized but also acted as a chain transfer agent due to the polymerizable vinyl group and sulfonyl chloride chain transfer group in the VBSC. Gel permeation chromatography (GPC) traces demonstrated that the number-average molecular weights and molecular weight distributions increased along with monomer conversion. Compared with the RAFT process without VBSC, the resulting polymers had broad molecular weight distributions and the sulfonyl functionality of the resultant polymer at the branching point, indicating the formation of branched polystyrene. The structure of the obtained polystyrene was further analyzed by proton nuclear magnetic resonance (1H NMR) spectroscopy. The findings indicated that the branched polystyrene was mainly formed via the RAFT copolymerization of VBSC and styrene firstly to form polystyrene bearing pendant sulfonyl chloride group, and then the pendant sulfonyl chloride group acted as the chain transfer agent to generate the branched structure. In addition, the degree of branching and VBSC unit in copolymer increased along with the VBSC in the feed, implying that composition and structure of the branched polystyrenes could be tuned by the amount of VBSC in the feed.