전통주 주박의 항혈전 활성 평가

김미선(Mi-Sun Kim),이예슬(Ye-Seul Lee),김종식(Jong Sik Kim),신우창(Woo-Chang Shin),손호용(Ho-Yong Sohn) 한국생명과학회 2014 생명과학회지 Vol.24 No.8

전통주 주박을 이용한 고부가가치 식품소재 개발을 위해, 상업적 시설에서 생산된 3종 약주(J-B, J-S, J-Y) 및 2종 탁주(J-H, J-W) 주박의 ethanol 추출물 및 열수 추출물을 조제하고 이들의 혈액응고 저해활성, 혈소판 응집저해 활성 및 인간 적혈구 용혈활성을 평가하였다. 5종 주박의 pH는 3.90~4.29로 유사하였으나, brix는 5.0~27.0으로 다양하게 나타났으며, 수분 및 알코올 함량에서도 시료에 따라 1.8배의 차이를 나타내었다. 주박의 색차와 성분은 첨가된 부재료 및 사용누룩에 좌우되었으며, J-W 주박의 경우 수분함량이 80.3%, brix 13, 알코올 함량 1.8%를 함유하여 다른 주박에 비해 다양한 식품제조에 용이하게 이용 가능하리라 판단되었다. Ethanol 추출효율은 J-H, J-W, J-B, J-S, J-Y의 순, 열수 추출효율은 J-S, J-B, J-W, J-H, J-Y의 순으로 높았으며, 총폴리페놀 및 총플라보노이드함량은 ethanol 추출물 중에서는 J-H, 열수 추출물 중에서는 J-Y 주박에서 가장 높았다. 5종 주박의 10종 추출물은 모두 5 mg/ml 농도까지 인간 적혈구에 대한 용혈활성이 나타나지 않았으며, J-B, J-S, J-Y의 약주 주박의 ethanol 추출물에서 유의적인 혈액응고저해 활성이 나타났으며, J-W 탁주 주박의 열수 추출물에서 thrombin 저해 활성과 J-B, J-S 및 J-H 주박 열수 추출물에서 혈액 응고인자 저해활성을 확인하였다. 혈소판 응집저해 활성평가의 경우 J-W 탁주 주박의 ethanol 및 열수 추출물에서만 아스피린에 필적하는 우수한 활성이 확인되었다. 본 연구결과는 다양한 약주 및 탁주 주박이 항혈전 활성을 가지고 있으며, 주박으로부터 항혈전제 개발이 가능함을 제시하고 있다. In this study, ethanol and hot water extracts of lees from Korean traditional wine (J-B, J-S, J-Y, J-H, and J-W) were prepared, and their effects on blood coagulation, platelet aggregation, and hemolysis of human red blood cells (hRBCs) were investigated to develop functional food ingredients from lees. The pH and brix of the lees ranged from 3.90 to 4.29 and 5.0 to 27.0o, respectively, and there was a huge difference in the water and ethanol content among the lees. The nuruk and additives used affected the color and physicochemical properties of lees. The J-W takju made from only rice and traditional nuruk, which has 13o brix and 1.8% of alcohol, has potential as functional food ingredient. With regard to the extraction yields of lees, higher yields were obtained from J-H, which contains different medicinal plants, in ethanol, followed by J-W, J-B, J-S, and J-Y. Higher extraction yields of lees were obtained from J-S in hot water, followed by J-B, J-W, J-H, and J-Y, respectively. The ethanol extract of J-H and the hot water extract of J-Y had the highest contents of total polyphenol and total flavonoids among the lees extracts. The 10 lees extracts did not show hemolysis activity against hRBCs up to 5 mg/ml. In an anticoagulation activity assay, the ethanol extracts of three yakju lees (J-B, J-S, and J-Y) and the hot water extract of J-W inhibited thrombin activity, whereas the hot water extract of J-B, J-S, and J-H inhibited blood coagulation factors. In an antiplatelet aggregation activity assay, only the J-W takju lees showed significant inhibition activity. Our results suggest that lees from traditional wine had high potential as a novel antithrombosis agent.

Effect of Optimum Nutrient Management on Productivity and Nitrogen Balance in Rice Cultivation: A Review

Seul-Bi Lee,Yang-Min Kim,Ye-Jin Lee,Yo-Sung Song,Cheol-Hyun Ryu,Deog-Bae Lee,Chan-Wook Lee,Chang-Hoon Lee,Jwakyung Sung 한국토양비료학회 2019 한국토양비료학회지 Vol.52 No.4

Given continuous needs for a public duty of agriculture and rural area, an effort to improve nutrient management in agricultural sector is strongly required. As a measure to solve these problems, Korean government (MAFRA) introduced the promotion policy called as “Agro-Environment Conservation Program (AECP)” in 2018 and has expanded 5 projet sites in 2019 from 3 in 2018. The program has a variety of agricultural activity to conserve soil, water, air, ecology and landscape. Of those, the nutrient input activity which is one of sub-activities is closely involved in not only crop productivity but also water quality and air pollution. Therefore, in order to lead a soft landing of AECP, it is essential to evaluate the conservation effects in a scientific point of view. In this respect, we focused on evaluating the effect of recommended nutrient input activities, standard fertilization and soil test fertilization, on rice productivity and nitrogen balance. The effect of nutrient management which is explained by rice yield and nitrogen balance was estimated with the combination of soil characteristics and rice varieties. Rice yield by recommended fertilization was 654 kg per 10a, 6% lower than control, but there was no statistical significance. By contrast, nitrogen balance was 4.7 kg lower, which means great improvement, in the recommended than the control. Conclusively, the recommended fertilization like soil test fertilization is expected to be useful as a measure to reduce nutrient balance, to ensure crop productivity, and finally to perform a public role of agriculture.

Identification of determinants of the utilisation of acupuncture treatment using Andersen's behavioural model

Lee, Ye-Seul,Lee, In-Seon,Kim, Song-Yi,Lee, Hyangsook,Park, Hi-Joon,Lee, Hyejung,Kim, Sung-Hoon,Chae, Younbyoung THE BRITISH MEDICAL ACUPUNCTURE SOCIETY 2015 ACUPUNCTURE IN MEDICINE Vol.33 No.2

<P><B>Objectives</B></P><P>The utilisation of healthcare services is the result of a complex decision-making process with different determinants. This study aims to identify the determinants of the utilisation of acupuncture treatment using Andersen's behavioural model and quality of life.</P><P><B>Methods</B></P><P>In this study we reviewed Korean Health Panel data, a nationally representative set of data, and analysed the utilisation of outpatient acupuncture services during 2011. A two-part model, which included predisposing, enabling and need factors of Andersen's behavioural model in model 1 and the additional factor of health-related quality of life (HRQOL, EQ-5D Korean index) in model 2, was analysed by logistic analysis to identify determinants of acupuncture service usage among subjects who visited traditional Korean medical clinics.</P><P><B>Results</B></P><P>The higher frequency of acupuncture service usage among patients who visited traditional Korean medical clinics was associated with gender, age, number of chronic diseases, physical disabilities and HRQOL of the subjects.</P><P><B>Conclusions</B></P><P>This study shows that the important determinants of the frequency of acupuncture treatment utilisation are gender, age, number of chronic diseases and the presence of physical disabilities. Our findings contribute to understanding the characteristics of patients who use acupuncture treatment and may be used as a basic resource for related policy making by government officials and medical professionals.</P>

고구마 소주 주박의 항균 및 항혈전 활성

김미선 ( Mi Sun Kim ),이예슬 ( Ye Seul Lee ),김종식 ( Jong Sik Kim ),신우창 ( Woo Chang Shin ),손호용 ( Ho Yong Sohn ) 한국미생물생명공학회(구 한국산업미생물학회) 2014 한국미생물·생명공학회지 Vol.42 No.3

Sweet potato soju (SPS), a form of traditional distilled alcoholic liquor in Korea, is manufactured by the distillation of fermented broth under normal pressure, thus providing it for a uniquely smooth taste infused with the flavor of sweet potato. After distillation, the lees of SPS is produced as by-product and discarded. In this study, the ethanol and hot water extracts of lees of SPS, and their subsequent organic solvent fractions using hexane, ethylacetate (EA), butanol, and water residue were prepared in an effort at the efficient re-use of the lees of SPS. The ethanol extraction yield was 1.36-fold higher than that of the hot water extraction, and the EA fraction revealed the highest total polyphenol content among the solvent fractions. The various extracts and solvent fractions did not demonstrate hemolytic activity at up to 0.5 mg/ml concentrations against human red blood cells. In the bio-activity assay, only the EA fraction displayed a broad spectrum of anti-microbial activity against different pathogenic and food spoilage bacteria, and demonstrated significant anti-coagulation activity by inhibitions of thrombin, prothrombin and blood coagulation factors. Furthermore, only the EA fraction from the hot water extract of the lees of SPS showed anti-platelet aggregation activity, which is comparable to aspirin (a commercially available drug). Our results suggest that the EA fraction of the hot water extract prepared from the lees of SPS has a high potential as a novel resource for anti-microbial and anti-thrombosis agents.

Nanoencapsulation of Combined Antimicrobial Compounds for Improving Oral Health

Ye-Seul Choi,Eun Sung Cho,Eun Suh Kim,Ji-Soo Lee,Hyeon Gyu Lee 한국식생활문화학회 2017 한국식생활문화학회학술대회논문집 Vol.2017 No.11

Comparison of Titratable Total Acidity of Alcoholic Beverages by Four Different Methods

Ye-Seul Kim,Bo-Ram Na,A-Young Lee,Jeung Hee Lee 한국식품영양과학회 2015 한국식품영양과학회 학술대회발표집 Vol.2015 No.8

Growth Stage-Based Fertigation Guideline for Greenhouse Spring Chinese Cabbage

Seul-Bi Lee,Yang-Min Kim,Ye-Jin Lee,Yo-Sung Song,Deog-Bae Lee,Jwakyung Sung 한국토양비료학회 2019 한국토양비료학회지 Vol.52 No.4

With increasing fertigation, the precise fertigation schedule by crop growth stage has been consistently required as a guideline for stable crop production. The aim of this study is to develop the fertigation guideline for spring cabbage. To achieve this goal, we firstly investigated the nutrient uptake pattern of Chinese cabbage, which grown in pots filled with sandy loam soil in a greenhouse from April to June in 2018, and determined optimal nutrient (N, P and K) supply by growth stages. Based on soil NO₃-N based recommendation, nitrogen (N) supply was divided into 4 levels (0.5N, 0.75N, 1.0N and 1.5N). Soil NO₃-N concentrations somewhat differed from growth stages, and 1.5N represented the highest level. Chinese cabbage showed linear growth patterns and there was no clearly significant difference in growth and nutrient uptake by N supply levels except for 0.5N treatment. The relative growth (expressed as a dry weight) of Chinese cabbage occupied 60% and 40% of total accumulated growth (average of 0.75 and 1.0N) at 40 and 60 days, respectively. Mineral uptake (N, P, K) of Chinese cabbage by growth stages showed a linear pattern in general. N was taken up 54% and 46% of total uptake, P uptake 56% and 44%, and K uptake 59% and 41% at 40 and 60 days, respectively. Conclusively, we carefully suggest that the fertigation guideline for Chinese cabbage could save fertilizer supply through daily nutrient demand and target yield, and thus alleviate the problems in relation to nutrient accumulation in greenhouse soils.

Prediction model for utilization of complementary and alternative medicine for sports injuries among Korean elite collegiate athletes

Ye-Seul Lee,박득수,Jae Keun Oh,Song-Yi Kim 한국한의학연구원 2020 Integrative Medicine Research Vol.9 No.2

Background: The purpose of this study was to describe the use of complementary and alternative medicine (CAM) services by Korean elite collegiate athletes and to examine the factors influencing the utilization of CAM, or Korean Medicine (KM). Methods: Students in Korea National Sport University (KNSU) enrolled and in training during August to December 2017 completed the survey. Data from the survey was analyzed through path analysis. Results: Students who were training in the Department of Physical Education, KNSU (n = 624, 70% males, mean age 21.3 ± 1.2) participated in this study. 15% of the respondents chose KM clinics for sports injuries, a higher rate than that of the general population, and 44% of KM interventions involved acupuncture. In the path analysis, sports injuries were positive predictors for utilization of medical services and the perception of KM, and KM utilization and the perception of KM were positive predictors of the willingness to receive KM treatments. Conclusions: The use of KM such as acupuncture was relatively high in elite collegiate athletes. The perception of KM and willingness to receive KM treatments were mediated by previous KM experiences. A higher accessibility towards KM treatments are necessary.

How do readers choose to undergo treatments based on medical articles?

Lee, Ye-Seul,Kim, Jeongjoo,Joo, Seongsu,Go, Byeongho,Lee, Hyangsook,Chae, Younbyoung Williams & Wilkins Co 2016 Medicine Vol.95 No.49

<P><B>Abstract</B></P><P>The purpose of this study is to study the reader's cognitive process in reading medical articles and its influence on the decision-making process. Twenty-four participants completed a survey pertaining to 36 medical articles on new treatments with similar text structures and similar titles. Participants rated each article on its level of interest, informativeness, and reliability, and were asked whether they would choose the treatments in the news article. A cognitive decision-making model can be applied to health contexts, in which the reader's subjective ratings on interest, informativeness, and reliability were positively associated with choosing new treatments. The decision-making process path from the perception of informativeness was mediated by the reliability of the news article. Interest was positively linked to informativeness, although it was not directly associated with reliability. This study shows that readers’ subjective ratings on health information can indicate their decision-making. Artifacts in the information that might incite emotions or interest, such as framing, can affect the reader's decision-making process.</P>

Construction of Science Museum Platform for Sharing and Exchange of Insects Exhibit Contents

Ye-Seul Kim,Da-Som Kim,Ju-Hee Lee,Se Ryeong Lee,Bong-Kyu Byun 한국응용곤충학회 2019 한국응용곤충학회 학술대회논문집 Vol.2019 No.04

국내 과학관 수는 지난 10여년 동안 양적으로는 팽창하고 있으나, 대부분이 중소규모의 과학관으로 인력부족 및 과학전시 컨텐츠 부족에 따라 운영에 어려움을 겪고 있다. 이에 따라 과학관이 보유하고 있는 전시 컨텐츠의 DB구축 및 정보 공유를 통해 과학관간 상호 교환 및 교류를 촉진할 수 있는 과학관 전시 플랫폼 구축이 요구되고 있는 실정이다. 국외 선진 과학관의 경우 자연사 컨텐츠의 전산화 및 DB 구축을 통해 대국민 검색서비스를 제공하여 전시품 등을 다양하게 활용하고 있는 추세이다. 따라서 본 연구에서는 과학관에서 활용가능한 자연사 컨텐츠 중, 곤충을 중심으로 이들의 전시 컨텐츠 발굴 및 DB확보 현황과 향후 플랫폼 구축 계획에 대해 소개하고자 한다.