중증 재생불량성빈혈에서 타인 혹은 HLA 불일치 혈연간 골수이식을 위한 cyclophosphamide와 antithymocyte globulin전처치 요법

이정희,이제환,김신,설미이,이정신,김우건,김상희,지현숙,박찬정,이규형 대한조혈모세포이식학회 2001 대한조혈모세포이식학회지 Vol.6 No.1

배경: Cyclophosphamide와 ATG의 전처치 요법은 HLA가 일치하는 형제간 골수이식에서는 매우 효과적인 것으로 알려져 있으나, 타인 혹은 HLA 불일치 혈연간 골수이식에서는 전처치 요법으로서 불충분한 것으로 보고되었는데, 주요 문제는 착상 부전이었다. 우리나라를 포함하는 극동아시아 지역의 국가들에서 발표되는 보고에 의하면 타인이식을 포함하여 골수이식 후 착상 부전과 이식편대숙주질환의 발생이 서구 국가들로부터의 보고에서보다 적은 것으로 알려져 있다. 저자 등은 6예의 중증 재생불량성빈혈 환자들에서 질병 초기에 Cyclophosphamide와 ATG만을 이용하여 전처치를 한 후에 타인 혹은 HLA 불일치 혈연간 골수이식을 시행하였다. 방법: 1999년 5월부터 2000년 4월까지 서울중앙병원에서 타인 혹은 HLA 불일치 혈연간 골수이식을 시행 받은 성인 중증 재생불량성빈혈 환자들을 대상으로 하였다. 골수이식을 위한 전처치 요법으로 cyclophosphamide (50 mg/kg/d x 4)와 ATG (30 mg/kg/d x 3)를 투여하였는데, 골수이식 전에 ATG에 대하여 과민 반응을 보였던 한 예 (UPN 120)에서는 ATG 대신에 fludarabine (30 mg/m2/d x 3)을 투여하였다. 이식편대숙주질환의 예방을 위하여 cyclosporine과 methotrexate를 투여하였다. 결과: 연구 기간 중에 모두 6예가 타인 혹은 HLA 불일치 혈연간 골수이식을 시행 받았는데, 5예는 타인으로부터 골수를 공여 받았으며, 1예는 HLA의 표현형이 하나의 유전자좌에서 일치하지 않는 형제로부터 골수를 공여 받았다. 타인 골수이식을 받은 5예 중에서 3예의 공여자는 HLA의 표현형이 일치하였으며, 1예는 하나의 유전자좌가 major mismatch였고, 1예는 하나의 유전자좌가 minor mismatch였다. 추적기간의 중앙값은 406일 (범위, 328-643일)인데, 6예 모두에서 착상된 상태를 유지하면서 생존해있다. 2예에서 3도의 급성 이식편대숙주질환과 전신성 만성 이식편대숙주질환이 발생하였다. 결론: Cyclophosphamide와 ATG의 전처치 요법은 재생불량성빈혈의 초기에 타인 혹은 HLA 불일치 혈연간 골수이식을 시행 받은 한국인 환자들에서 충분히 착상을 가져올 수 있는 요법으로 생각된다. 비록 본 연구의 대상 환자 수가 적지만 결과는 매우 고무적이다. HLA가 일치하는 형제가 없는 중증 재생불량성빈혈 환자의 경우에 타인 골수 공여자에 대한 검색을 조기에 시행해야 하며, 타인 혹은 HLA 불일치 혈연간 골수이식을 질병 초기에 적극적으로 고려해야 할 것으로 생각된다. Background: Cyclophosphamide (CY) and ATG regimen was reported to be insufficient for alternative donor BMT in SAA patients due to high incidence of graft rejection. The rates of graft rejection and GVHD after allogeneic BMT including unrelated donor transplants seemed to be lower in reports from Asian countries of Far East. We applied CY/ATG regimen to six patients with early stage of SAA transplanted with marrow from alternative donors other than HLA-identical siblings. Methods: Three patients were transplanted from HLA-phenotypically identical unrelated donors, two from one-locus mismatched unrelated donors, and one from an one-locus mismatched sibling donor. Four patients received no previous therapy for SAA, and two received one course of immunosuppressive therapy with ATG. Preparative regimen was CY (200 mg/kg) plus ATG (90 mg/kg) in all patients except one who had had an anaphylactic reaction to ATG prior to BMT and received fludarabine (90 mg/m2) in place of ATG. Cyclosporine plus methotrexate were given for GVHD prophylaxis. Results: All six patients engrafted and all are alive with durable engraftment at a median follow-up of 406 days (range, 328 to 643). Two patients developed grade III acute GVHD and extensive chronic GVHD. Conclusions: CY/ATG regimen may be sufficient in Korean patients with early stage of SAA transplanted with marrow from alternative donors. Although the number of patients in present study is small, results are encouraging. Stem cell transplantation using alternative donors may be considered at early stage of aplastic anemia.

고품질 복숭아 만생종 ‘진백’ 육성

이기우(Gi-Woo Lee1),박원흠(Won-Heum Park),권오흔(Oh-Heun Kwon),정경미(Kyung-Mi Jung),이형일(Hyung-Il Lee) 한국원예학회 2018 한국원예학회 학술발표요지 Vol.2018 No.10

림프종 환자에서의 갈륨, TI-201 그리고 Tc-99m MIBI 섭취의 비교

천경아,조인호,원규장,이경희,이형우,현명수,이재태,이규보 영남대학교 의과대학 2002 Yeungnam University Journal of Medicine Vol.19 No.2

Purpose; Ga-67 scintigraphy has been used for the evaluation of tumors, especially lymphoma. Recently, Tl-201 and Tc-99m MIBI were also used to tumor imaging. Tl-201 and Tc-99m MIBI had better physiologic characteristics than Ga-67, so we studied 32 biopsy proven lymphoma patients (male 24, female 8, mean age 46 years) with Ga-67, Tl-201 or Tc-99m MIBI and compared the scan findings. Materials and Methods: Twenty-three of 32 patients were injected 74-111 MBq (2-3 mCi) of Tl-201, before chemotherapy and imaged with dual-headed SPECT (Prism 2000, Picker, USA) at 30 minutes after injection. Delayed images were obtained after 3 hr in 8 patients. Twenty seven of 32 patients were injected 740 MBq (20 mCi) of Tc-99m MIBI and imaged at 30 minutes after injection. 111-185 MBq (3-5 mCi) of Ga-67 was injected in 12 patients and imaged at 48 and 72 hours after injection. Twenty eight patients were diagnosed as non-Hodgkin's lymphoma and others were Hodgkin's lymphoma. Results: Twenty patients were positive on Tl-201 scan and 3 patients showed negative findings. One of these 3 patients, Tc-99m MIBI and Ga-67 scan were positive. Twenty two patients were positive on Tc-99m MIBI scan and 5 patients showed negative findings. One of these 5 patients, Tl-201 was positive and 2 were positive on Ga-67 scan. Ten of 12 patients showed positive findings on Ga-67 scan. The sensitivity of these agents were 83.3%, 87.0% and 81.5% for Ga-67, Tl-201 and Tc-99m MIBI, respectively. The sensitivity was highest in Tl-201 scan, but there were no significant differences among three tests. In this study, there was no significant difference of uptake ratios between early and delayed images of Tl-201. Conclusion: Scintigraphy with Tl-201 and Tc-99m MIBI in lymphoma patients have similar sensitivity with Ga-67.

관상동맥 질환에서 SDF-1α의 농도

김보영,박용규,박형서,노상필,정승현,이유선,이정우,이재환,최시완,정진옥,성인환 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

Atherosclerosis is now viewed as an inflammatory disease of the vascular system. Expression of several chemokines, including monocyte chemoattractant protein(MCP)-1, MCP-4, RANTES(regulated on activation normal T-cell expressed and secreted), and interleukin-8(IL-8) are increased in human atherosclerotic plaques compared with normal vessels. They are involved in the pathogenesis of atherosclerosis and plaque rupture by activating and directing leukocytes into the atherosclerotic lesions. However, some are involved in homeostatic functions such as normal leukocyte traffic and growth regulation. SDF-la is a multi-functional cytokine that is involved in myelogenesis, hematopoiesis, angiogenesis and injured gastric mucosal regeneration in the gastric ulcer patient. SDF-la is recently shown to be highly expressed in atherosclerotic plaques and a potent platelet agonist. At least in high concentrations, SDF-la may mediate antiinflammatory and matrix stabilizing effects in unstable angina. Many studies are going on to know the function of SDF-la in coronary artery diseases. I investigated the difference of the plasma level of SDF-la between control group and coronary artery disease group. Total 75 subjects were enrolled. The diagnosis of coronary artery disease was confirmed in all patients by coronary angiography. Control subjects in this study were confirmed normal by coronary angiography. Clinical profile and risk factors were also reviewed. Control subjects in this study were 27 (M=10, F=17). Plasma for the study was collected before the angiography and centrifuged. SDF-la analysis was performed by ELISA. Plasma level of SDF-la is significantly increased in patients with stable angina(n=20) and unstable angina group(n=28) compared with healthy control group(n=27). the risk factors do not influence the plasma level of SDF-la in coronary artery diseases. In this study, plasma level of SDF-la is increased in patients with stable angina and unstable angina groups compared with healthy control group(P<0.05). the risk factors do not influence the plasma level of SDF-la in coronary artery diseases.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

여성 요도증후군에서 Terazocin의 효과에 대한 연구

우승효,신관희,한광희,정원일,이형래,이상철,김용태,김원재 충북대학교 의학연구소 2000 忠北醫大學術誌 Vol.10 No.2

연구목적: 여성 요도증후군을 보이는 환자에서 교감신경차단제인 terazocin을 복용한 후 치료 효과에 대한 각 증상의 변화와 부작용을 알아보고자 하였다. 대상 및 방법: 1997년부터 1999년까지 충북대학교병원 비뇨기과에 내원한 35명의 여성 중 요검사 및 요배양 검사, 그리고 다른 이학적 검사에서 이상이 없는 요도증후군 환자를 대상으로 간질성방광염 증상점수(ICSS)를 terazocin (3mg/day for 3 months) 투여 전후에 조사하였고, 치료 후 3개월과 1년에 Likert scale 점수를 조사하였다. 결과: ICSS는 비치료군은 18.3±5.9점, 치료군은 16.5±6.8점으로 두군간의 차이는 없는 것으로 나타났다(p=0.52). 치료군은 치료 후 3개월에 시행한 ICSS가 9.9±7.8점으로 치료 전에 비해 현격히 증상이 호전되는 것으로 나타났다(p=0.0001). 특히 3개월에 시행한 Likert scale에서는 주간 및 야간빈뇨, 절박뇨, 배뇨통의 횟수 및 강도, 그리고 전체적인 증상정도에서 40-70% 가량 호전된 것으로 밝혀졌으며 이는 1년 후에도 증상의 호전이 지속되는 것으로 나타났다. 비치료군에서의 1년 후 Likert scale은 6명 중 2명이 전체적인 증상의 호전이 있는 것으로 나타났다. 결론: Terazocin은 환자의 약 70%가량에서 증상의 호전을 가져왔고, 비치료군에서도 환자의 33%가 저절로 증상이 좋아진 것으로 보아 Terazocin은 여성 요도증후군에서 분명히 효과가 있으나 증상이 심할 경우 보조적인 치료로 이용되는 것이 바람직할 것으로 사료된다. Purpose: We performed to assess the effects and complications of terazocin in women with chronic female urethral syndrome. Materials and Methods : Thirty-five women with chronic female urethral syndrome were studied from March 1997 to August 1999. The subjects exhibited normal findings without the pyuria and bacteriuria in urinalysis and urine culture and were invested the interstitial symptom score(ICSS). 29 subjects of them took a terazocin 1-4 mg/day for 3 months. They were reevaluated for the ICSS and Likert scale after 3 months and for Likert scale after 1 year. Results: The ICSS before treatment were showed 18.3±5.9 in the untreated patient group and 16.5±6.8 in the treated patient group, and no statistically difference between both patient groups(p=0.52). In the treated patient group, the ICSS after treatment were showed 9.9±7.8, significant difference between before and after treatment(p=0.0001). The Likert scale was showed at 3 months after treatment that frequency, nocturia, urgency, pain frequency, pain intensity, daily activities, and overall symptoms were improved in 65.5%, 55.2%, 24.1%, 41.4%, 41.4%, 70%, 70%, and 70% of 29 subjects, each other. Overall symptom score assessed at 1 year were showed that 3 subjects was aggravated. In untreated patient group, 2 of 6 was improved in overall symptom score. Conclusion: Terazocin was effective above 70% in the treated group, and symptoms were improved spontaneously in a third of the untreated group. So, we thought that terazocin would be useful as supportive treatment in period of severe symptoms.

법랑아세포종에서의 p53 및 MIB-1 발현 : 임상 및 병리학적 요인들간의 연관성 Correlation Between Clincopathologic Parameters

이수운,한지용,권창석,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Thirty five patinets' paraffin embedded biops block , which was previously diagnosed ameloblastoma and obtained from oral and maxillofacial surgery dept. of pusan paik hospital and OMS dept of collage dentistry, pusan national university hospital, from January 1994 to march 1999, were performed immunohistochemistry with p-53 and MIB-1. On investigating the expression, correlation between clinical and histologic factor and correlation between prognosis and moleculopathologic property were examined. And we obtained the following results; 1. Age over forty, recurrence rate was 58.5%. 91.4% of the tumor was developed on mandibule, recurrence rate of maxilla area tumor was 66.7% On radiologic and histologic feature, multilocular and follicular type has high recurrence rate but no significance statistical difference was found 2. Expression of p-53 was 25.7%, When underage of forty. location on mandibule, multilocular and plexiform type, the expression of p-53 was somewhat higher and recurred cases of tumor, expression rate was 12.5%. 3. MIB-1 expression rate was 77.1%. When location on mandibule, unilocular, plexiform type tumor and previously recurred case, MIB-1 expression was 87.5% which was somewhat higher than no recurrence case of 74.1%. 4. 33.3.% of positive on MIB-1 have expression of p-53 and all p-53 positive case express MIB-1 positive. All p-53 positive case have high cell proliferation rate. From this result, age and location have closely related with the prognosis of tumor, histologic feature and MIB-1 expression has related with the prognosis of tumor. But p-53 has highly expressed on cell proliferation area, not closely related with the prognosis of tumor.

네트워크 분산반사 서비스 거부 공격(DRDoS)에 대한 역추적 시뮬레이터 설계 및 구현

이형우 한국컴퓨터교육학회 2005 컴퓨터교육학회 논문지 Vol.8 No.1

흰쥐 내측 전정신경핵 뉴론의 자발적 활동성의 발생에 관여하는 이온전류에 관한 연구

이형재,최정희,천상우 원광대학교 치의학연구소 2003 圓光齒醫學 Vol.12 No.2

Medial vestibular nucleus (MVN) neurons are found to have spontaneous electrical activity in the absence of any detectable synaptic input. To investigate the contributions of intrinsic mechanisms to the spontaneous activity of MVN neurons, we examined the effects of various channel blockers on spontaneous firing by means of patch clamp recordings. Coronal slice (400 ㎛) of the vestibular nucleus region was sequentially treated with pronase 0.2 ㎎/㎖ and thermolysin 0.2 ㎎/㎖, then single neurons were mechanically dissociated. Almost all the MVN neurons recorded in neonatal rat were shown to have spontaneous resting activity. In 300 nM TTX, spontaneous firing was blocked in all cells tested. In 8 of 11 cells, underlying fluctuation or oscillations in membrane potential was not remained, and hyperpolarization did not produce rebound low-threshold calcium spikes. Although MVN neurons possessed hyperpolarization activated cation current (Ih), cesium had no effect on firing rates. The spike afterhyperpolarization (AHP) is calcium dependent. When Ca^2+ influx was blocked in external Ca^2+ free solution, repetitive firing was abolished and the cell rested at depolarized membrane potentials. Application of apamin (300 nM) caused a profound reduction in the amplitude of the AHP and produced rhythmic burst firing. These findings suggest that the spontaneous activities of MVN neurons is regulated by interactions between the membrane depolarization mainly due to a persistent sodium conductances and hyperpolarization due to the calcium-activated potassium conductances.