中風(腦卒中)의 臨床的 硏究

李仁仙,張仁圭 慶熙大學校韓醫科大學韓醫學硏究所 1985 慶熙韓醫大論文集 Vol.8 No.-

오매(烏梅)의 다성분동시분석 및 마우스를 이용한 경구 단회투여 급성독성시험

이인선,한창현,이철,황방연,정상혁,이영준,전원경,Lee, In-Sun,Han, Chang-Hyun,Lee, Chul,Hwang, Bang-Yeon,Jung, Sang-Hyeok,Lee, Young-Joon,Jeon, Won-Kyung 대한예방한의학회 2011 대한예방한의학회지 Vol.15 No.1

Objectives : Previous studies have shown that Fructus mume (FM) has anti-platelet effects. The present study was performed to determine the acute oral toxicity and quality control of a crude extract of FM in ICR mice. Methods : We investigated the in vivo single dose acute toxicity of FM 95% ethanol extract. This test was orally administered once by gavage to 20 male and 20 female mice at dose levels of 0 (control group), 1250, 2500 and 5000mg/kg body weight, respectively. Mortalities, clinical findings, autopsy findings and body weight changes were monitored daily for the 14 days following the administration. HPLC analysis was performed for the simultaneous determination of ursolic acid and p-hydroxycinnamic acid in FM. Reverse-phase chromatography using a C18 column and photodiode array detection at 211 nm was used for quantification of the two maker components. The mobile phase for gradient elution consists of water and acetonitrile. Results : We observed survival rates, general toxicity, change of body weight, and autopsy. The mice did not die after single oral administration of maximum dose of FM. Autopsy of animal revealed no abnormal gross finding. Therefore, $LD_{50}$ value of FM for ICR mice was more than 5000mg/kg on oral route. The HPLC analysis showed that ursolic acid and p-hydroxycinnamic acid amounts to 9.75- and 0.12% in the extract with the retention times of 47.99- and 15.38 minutes, respectively. Conclusions : These results suggest that no toxic dose level of FM in mice is considered to be more than 5000mg/kg. Consequently, it was concluded that FM have no effect on acute toxicity and side effect in ICR mice. For the quality control of FM extract, simultaneous determination of ursolic acid and p-hydroxycinnamic acid was established.

Gelatinases of Extracellular Matrix of Human Oocyte-Cumulus Complex

이인선,나경아,김해권 한국발생생물학회 2001 발생과 생식 Vol.5 No.2

포유동물의 난포내 난자의 성숙 시에는 난자를 둘러싸고 있는 난구세포의 확장 현상이 일어나는데 이 현상에는 hyaluronic acid 뿐만 아니라 다른 성분도 관여하는 것으로 알려져 있다. 본 연구는 조직 재구성 과정에서 중요한 역할을 하는 matrix metalloproteinase(MMP)가 사람의 성숙한 난자-난구 복합체의 extracellular matrix(ECM)에 존재하는지의 여부를 알아보고자 하였다. 체외수정 시술 시에 얻어지는 사람의 난 When mammalian oocytes undergo maturation, cumulus cells surrounding the oocyte exhibit remodeling of their structure known as cumulus expansion. Many molecules including hyaluronic acid participate in this remodeling. The present study aimed to investigate a possible existence of matrix metalloproteinases(MMPs) in the extracellular matrix(ECM) of human oocyte-cumulus complex. ECM was extracted from the human oocyte-cumulus complex. Gelatin gel zymogram of ECM exhibited 7 gelatinases having molecular weight of 300kDa, 240kDa, 200kDa, 180kDa, 116kDa, 97kDa, and 84kDa. This gelatinase profile was very different from that of ovarian mural granulosa cell extract or white blood cell extract, indicating that the oocyte-cumulus complex donating ECM was free from other than cumulus cells. When ethylenediaminetetraacetic acid or 1', 10'-phenanthroline was added to the reaction buffer during zymographic development, almost gelatinase activities were abolished, suggesting that they were MMPs. Following incubation of ECM in the presence of aminophenylmercuric acetate, an activator of proMMPs, 4 gelatinases of 240kDa, 180kDa, 97kDa, and 84kDa disappeared with the concomitant appearance of 80kDa, 65kDa, and 60kDa gelatinases. Based upon these observation, it is suggested that ECM of the human oocyte-cumulus complex consists of gelatinases, presumed to be MMP-2 and MMP-9 isoforms.

삼백초(Saururus Chinensis (Lour.) Bail) 열추출물의 항암 및 세포독성 저해 효과

이인선 한국식품저장유통학회 2001 Food Science and Preservation Vol.8 No.2

Chemoprevetive effect of Saururus Chinensis (Lour.) Bail water extract on several tumor cells and Chinese hamster V79 cells were investigated. The water extracts of Saururus Chinensis (Lour.) Bail showed a higher cytotoxicity effect on the human histiocytic leukemia cells(U937) and protective effects against the cytotoxicity of H$_2$O$_2$. These results suggest that Saururus Chinensis (Lour.) Bail may useful as potential soures of chemopreventive and antioxidative agents.

어드벤처 게임의 퍼즐 시스템이 플레이어의 게임 몰입과 참여도에 미치는 영향

이인선 한국게임학회 2008 한국게임학회 논문지 Vol.8 No.4

본 연구는 비디오 게임 구조와 플레이어 의식의 순환적 시스템에 대한 밀도 있는 관찰과 분석을 위해 '어드벤처 게임(Adventure Game)'장르로 분석대상을 제한하였다. 어드벤처 게임 고유의 특징적 단위구조인 '퍼즐 시스템'을 분석하였고, 그 경험이 플레이어의 의식에 '완결 욕구'를 발생시켜 몰입과 참여도에 작용하는 메커니즘 연구에 중점을 두었다. 연구과정은 문헌연구와 직접 플레이 등을 통해 어드벤처게임과 그 구성 요소들의 본질적 특성 및 비디오 게임 플레이의 몰입 특성을 살펴보았으며, 어드벤처게임에서의 퍼즐 시스템을 정의하고, 이 시스템 내에서 퍼즐요소와 내러티브 요소가 유기적으로 작용하는 구조 및 플레이 과정을 분석하여 '퍼즐 시스템'과 '완결 욕구'에 대한 가설들을 수립하였다. 가설을 확인하기 위하여 극적인 컷 씬이 포함된 게임과 컷 씬이 제외된 게임단위를 제작하고 실험을 실시하여 차이를 조사하였으며, 게임 난이도의 수준차이와 실패지점의 위치 변화를 유도하여 게임의 해결여부, 실패지점에 따른 플레이어의 완결 욕구와 참여도 변화를 파악하였다. This study has made an in-depth analysis of the 'Puzzle System' which is a characteristic structural unit of the Adventure Game, and concentrates on the mechanism where the experience arouses in the player a 'desire (quasi-need) to finish' that leads to one's immersion and engagement in the game. In the course of conducting this study, documentary records were consulted and also this writer personally played video games to learn the component elements and fundamental characteristics of the Adventure Games and to experience how the player was immersed in the game. Thus, the Puzzle System in the Adventure Game was defined and an analysis was made of the structure and course of play where the puzzle part and the narrative part within the system organically interacted between them in the system. In order to confirm the formation and effects of 'desire to finish' shown in the hypothetic assumptions, two experiment-purpose game units were produced, one with dramatic cut scenes and the other without them, so that differences between them could be found. I also conducted an experiment to learn the generation and effect of the 'desire to finish' of the Puzzle elements by using different games with a varying degree of difficulty.

수질평가 지수산정에 관한 연구

이인선,이길철,신상철,정동일,최훈근 한국물환경학회 1988 한국물환경학회지 Vol.4 No.1

This study was carried out to grope for Water Quality Index (WQI) which is able to use as a practical method evaluating water quality concerned with degree of pollution. 8 variables for determining the pollution were selected such as pH, DOs, Water temperature (WT), BOD, SS, TC, NH₃-N and NO₃-N and the results are summarized as follow. 1. Each WQI value was calculated by exponential equations (graph) described below. At the range. of pH 3.0 to 6.8, Y=10^((0.08394 x pH + 0.74921)) and pH 8.5 to 11.7, Y=10^((-0.21843 x pH + 3.85664)) At the range of DOs 30 to 100%, Y=10^((0.00999 x DOs + 1.00147 )) and DOs 100 to 130%, Y= 10^((-0.02330 x DOs +4.32990)) At the range of WT 28 to 34℃, Y=10^((-0.11650 x WT + 5.26186)) At the range of BOD 0.8 to 10.0 ㎎/ℓ, Y=10^((-0.075975 x BOD + 2.06078)) At the range of SS 2.6 to 37 ㎎/ℓ, Y=l0^((-0.018891 x SS + 2.056673)) At the range of TC 50 to 200,000 MPN/100㎖, Y=10^((-0.19416 x log TC + 2.33007)) At the range of NH₃-N+NO₃-N 0.4 to 3.4㎎/ℓ, Y=10^((-0.23299 x (NH₃-N + NO₃-N) + 2.09320)), Where Y is index value of each water quality. 2. The weights of each variable were 0.04 for pH and WT, 0.11 for DOs, 0.18 for TC, 0.21 for BOD, SS and NH₃-N+NO₃-N, respectively.

한방진단시스템 DSOM의 진단결과에 따른 월경통 환자의 허실(虛實) 진단에 대한 연구

이인선,조혜숙,윤현민,정경근,김규곤,박지은,최선미,Lee, In-Sun,Cho, Hye-Sook,Youn, hyoun-Min,Jung, Kyoung-Keun,Kim, Kyu-Kon,Park, Ji-Eun,Choi, Sum-Mi 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.1

Purpose : This study was undertaken to make a diagnosis weakness and firmness (虛實) of Dysmenorrhea patients by diagnosis questionnaires system(Diagnosis System of Oriental Medicine-DSOM) Methods : The subjects were 58 volunteers who was suffering for dysmenorrhea, employed using Measure of Menstrual Pain (MMP) questionnaire. The had agreed to take part in this experiment, with didn't take any anodyne drugs. The MMP score by using 7 questions and the Menstrual Symptom Severity List(MSSL-D) was measured before and after menstruation cycle. Results and Conclusions : The findings of this study were as follows; 1. We examined Pathogenic Factor's frequency of DSOM, Coldness(寒) was 45 persons 80.36%, Damp(濕) was 40 persons 71.43%, Heart(心) was 37 persons 66.07%, Heat syndrom(熱) was 9 persons 16.07%, insufficiency of Yang(陽虛) was 6 persons 10.71%. 2. We divided Dysmenorrhea patients into two groups(weakness and firmness) by Results of DSOM, Firmness was 25 Persons 43.1%, Weakness was 23 persons 39.7%, Unknown was 10 persons 17.2%. 3. In estimation based on Measure of Menstrual Pain (MMP) questionnaire Severe menstrual pain is weakness, Mild menstrual pain is Firmness. 4. In estimation of coldness and heat syndrom, Coldness was 40 persons 69.0%, Heat syndrom, was 2 persons 3.5%, Possess both coldness and heat syndrom was 9 persons 15.5%.

사회적 기술 부족과 충동성으로 인해 또래갈등이 심한 분교아동의 상담사례

이인선 한국초등상담교육학회 2006 초등상담연구 Vol.5 No.1

여고생의 월경이상에 대한 조사연구

이인선,전란희 대한한방부인과학회 2003 大韓韓方婦人科學會誌 Vol.16 No.4

‘미각교육 및 미식 체험 프로그램 사례’에 대한 토론

이인선 한국식생활문화학회 2019 한국식생활문화학회학술대회논문집 Vol.2019 No.11