Timing and Evolution of the Most Recent Common Ancestor of the Korean Clade HIV Subtype B Based on Nef and Vif Sequences

Mi-Suk Kim,So-Young Jang,Chan-Seung Park,이건명,이동훈,이찬희 한국미생물학회 2009 The journal of microbiology Vol.47 No.1

Molecular phylogenetic studies of the HIV-1 isolated from Koreans have suggested the presence of the so- called “Korean clade”, which can be defined as a cluster free of foreign isolates. The Korean clade accounts for more than 60% of Korean isolates and exerts characteristic amino acid sequences. Thus, it is merited to estimate when this Korean clade first emerged in order to understand the evolutionary pattern of the Korean clade. We analyzed and reconstructed the most recent common ancestor (MRCA) sequences from nef (n=229) and vif (n=179) Korean clade sequences. Linear regression analyses of sequence divergence estimates were plotted against sampling years to infer the year in which there was zero divergence from the MRCA sequences. MRCA sequences suggested the Korean clade was first emerged around 1984, before the first detection of HIV-1 in Korea in 1985. Further studies on synonymous and nonsynonymous substitution rates suggested positive selection event for the Korean clade, while other subtype B had undergone negative to neutral evolution.

Complete genome sequence of Sphingobium sp. strain PAMC 28499 reveals a potential for degrading pectin with comparative genomics approach

So‑Ra Han,Sung‑Min Jang,Young Min Chi,Byeollee Kim,정상희,Yung Mi Lee,Jun Uetake,Jun Hyuck Lee,Hyun Park,오태진 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background Spingobium sp. PAMC 28499 is isolated from the glaciers of Uganda. Uganda is a unique region where hot areas and glaciers coexist, with a variety of living creatures surviving, but the survey on them is very poor. The genetic character and complete genome information of Sphingobium strains help with environmental studies and the development of better to enzyme industry. Objective In this study, complete genome sequence of Spingobium sp. PAMC 28499 and comparative analysis of Spingobium species strains isolated from variety of the region. Methods Genome sequencingwas performed using PacBio sequel single-molecule real-time (SMRT) sequencing technology. The predicted gene sequences were functionally annotated and gene prediction was carried out using the program NCBI nonredundant database. And using dbCAN2 and KEGG data base were degradation pathway predicted and protein prediction about carbohydrate active enzymes (CAZymes). Results The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons. Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases. CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae. These results confrmed that this Sphingobium sp. strain PAMC 28499 have similar patterns to RG I pectin-degrading pathway. Conclusion In this study, isolated and sequenced the complete genome of Spingobium sp. PAMC 28499. Also, this strain has comparative genome analysis. Through the complete genome we can predict how this strain can store and produce energy in extreme environment. It can also provide bioengineered data by fnding new genes that degradation the pectin

오감(五感)적 요소를 접목한 브랜드 스페이스 연출 계획 -마임 화장품 프레쉬가든을 중심으로-

김소현,임하영,장효실,최혜원,이진민,장미정,이성애 숙명여자대학교 산업디자인연구소 2017 숙명디자인학 연구 Vol.24 No.-

인간이 갖고 있는 감각 기관은 시각, 청각, 후각, 촉각, 미각의 총 다섯 가지로, 이를 모두 활용 할 때 이상적 공간 상호작용이 가능하다. 본 연구는 화장품의 공간 연출 계획 시 오감적 요소를 접목하여 브랜드 이미지를 구축하는 일련의 프로세스를 밝히는데 목적이 있다. 이에 본 연구는 2017년 3월~6월 15주간 숙명여대 환경디자인학과 <융합연출디자인> 수업에서 진행된 수업의 결과물로서 작업물을 정리하였다. 그 결과 본 연구는 제품이나 서비스를 직접 경험할 수 있도록 다양한 엔터테인먼트적 요소를 결합하여 기능적 만족감과 더불어 체험적 만족감 제공하는 오감적 디스플레이를 제안하였다. 본 연구는 오감을 활 용한 뷰티 쇼룸으로 감각을 공간에 어떠한 방식으로 적용할 것인지에 대한 정립을 했다는 데에 의의가 있다. 아울러 다양한 매체와 공간의 개념이 일반화 되어 가는 오늘날 기업 환경 속에서 오감적 체험을 기반으로 한 공간연출은 가상의 공간속에서도 유연하게 적용 가능한 디스플레이 요소로 확인되었다. 또한 본 연구는 향후 이와 관련된 쇼룸 공간 개발에 있어서 기초 자료로 활용될 것으로 기대된다.

서울 시내 4년제 남자 대학생의 우울과 흡연량, 흡연기간, 니코틴 의존도와의 관계

박지영,박소영,이미숙,백수진,신의경,예혜련,오승진,장윤정,조혜진,김수지 이화여자대학교 간호과학대학 2003 이화간호학회지 Vol.- No.37

Background: With reports on the relation between smoking and depression appearing since 1980, it has become clear that depression is one of the psychodynamics of smoking, though there has been little or no progress made in the study of whether or not there is indeed a relation between depression and nicotine dependence. Purpose: The purpose of the study was to investigate the effects of depression on the amount and period of smoking and nicotine dependence among university man student smokers. Method: In periodical examination between 14 and 21, May, 2003, using 170 university male. The CES-D(Center for Epidemiologic Studies Depression) Scale and the FTQ(Fagerstrom Tolerance Qustionnaire) were utilized to measure the level of depression and nicotine dependency. The amount of smoking was calculated based on the number of cigarette used. Result: Results indicated that depression had something to do with amount of smoking. Nicotine dependence also was related to depression but total value was relatively low. finally the period of smoking had nothing to do with depression. Conclusion: The results of this study revealed that the amount of smoking of university man student smokers was very high. Both the amount of smoking and nicotine dependence were affected by levels of depression. Accordingly psychiatric nursing access such as emotional support is needed to manage depression and decrease smoking.

일본의 노인종합복지관 성공사례의 환경지원성 분석 연구

이연숙,이소영,여욱현,장미선 한국의료복지시설학회 2007 의료·복지 건축 Vol.13 No.1

According to the 2005 Korean census, the 65 and over population now exceeds 9.5% of the total population and is growing rapidly. Meeting elderly environment and care needs of this rapidly growing segment of the population becomes a major challenge for public policies and planners. Since great deal amount of elderly will reside in their houses, aging in place concept becomes important. For the success of aging in place, the quality of individual house unit, community support systems, and/or quality of senior center of the community are crucial. Since elderl environments and facilities serve not only medical and/or care programs but also social activity program in aging society, senior centers need to promote social activities and other care programs. The purpose of this study is to analyze characteristics of environmental affordance of a well received senior center in Tokyo, Japan. In order to analyze the characteristics, Murtha & Lee user benefit criteria and Lawton's environmental affordance approaches were used. As results, design characteristics and interior elements which provide environmental affordance were enumerated by type of space. Based on needs and user benefit criteria, those features were analyzed. This study shows design characteristics, elements, and attributes which are well received and utilized by elderl users.

Characterization of N-linked Glycan Structures Using Normal-phase Capillary LC-MALDI Tandem Mass Spectrometry

Jang Mi Jin,Jisun Yoo,So Young Jang,조건,Young Hwan Kim 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.3

Isolation and Identification of Anti-inflammatory Compounds from Radish Sprouts (Raphanus sativus L.)

Mi Jang,Gi Chang Kim,Jeong Yong Cho,In Guk Hwang,Byung Soon Hwang,Chan Mi Park,So Eun Park,Hab Hwa Beak 한국식품영양과학회 2019 한국식품영양과학회 학술대회발표집 Vol.2019 No.10

Solubilization of Chemically Reduced Graphene Oxide Using Coffee Catechol

Lee, Jang Mi,Jeong, Geumbi,Lee, Mi Yeon,Kim, So Yeon,Park, Young Ho,Park, Sung Young,Kim, Sang Chun,Min, Byung-Gak,In, Insik The Chemical Society of Japan 2013 Chemistry letters Vol.42 No.2

원재료로부터 분리된 α-, β-, γ-키틴의 물리화학적 특성과 약물 방출

장미경 ( Mi Kyeong Jang ),최창용 ( Chang Yong Choi ),최혜영 ( Hey Young Choi ),김태형 ( Tai Hyoung Kim ),손소희 ( So Hee Son ),장지태 ( Ji Tae Jang ),양현필 ( Hyun Pil Yang ),정특래 ( Teok Rae Jung ),강성구 ( Seong Koo Kang ),나 한국키틴키토산학회 2003 한국키틴키토산학회지 Vol.8 No.1

α-, β-, γ-키틴이 천연 재료로부터 분리되었고 이의 특성이 FT-IR spectrophotometer, solid state CP/MAS ^(13)C NMR spectrophotometer, TGA, XRD에 의해 확인되었다. 또한, 본 연구에서 분리한 키틴을 이용하여 서방성 제제로서의 가능성을 알아보기 위하여 약물전달체를 제조하였다. α-, β-, and γ-chitin의 점도평균분자량(Mvis)이 점도계를 이용하여 측정하였으며, 결과로써 각각의 분자량이 701, 612, and 524 kDa임을 확인하였다. FT-IR스펙트럼에서 아마이드 Ⅰ에서의 흡수밴드가 α-키틴에 있어서는 이중선으로, β-키틴에서는 단일선으로 나타났으며 γ-키틴에서는 α-, β-키틴의 중간형태로 나타났음을 확인하였다. Solid state CP/MAS ^(13) NMR 스펙트럼결과에서 α-키틴의 경우 C3과 C5의 피크가 각각 73과 75ppm에서 나타났으며 β-키틴은 74 ppm에서 단일선으로 나타났고, γ-키틴의 경우 C3과 C5의 흡수 피크가 α-키틴과 유사한 형태의 피크를 나타냈었다. X-ray회절 분석에서는 α-키틴의 경우 9.6, 19.6, 21.1, 23.7에서 4개의 결정면을 확인할 수 있었고, β-키틴의 경우 9.1, 20.3에서 2개의 결정면을 나타내었다. 또한 역평행의 구조와 평행의 구조가 혼재되어 있는 γ-키틴의 경우 β-키틴 보다 오히려 α-키틴에 가까운 결정성을 나타내었다. 그러나 400℃까지 가열하였을 경우 5°~35°에서 나타나던 of α-, β- and γ-chitin의 결정면이 사라졌음을 알 수 있었다. DSC 측정결과로써, α-, β-, γ-chitin의 구조적 특성으로 인해 각각 다른 발열 피크를 나타내었으며, 결과로써 α-chitin의 경우 330, β-chitin은 220 그리고 γ-chitin은 300℃임을 확인하였다. 또한, 본 연구에서 제조한 키틴을 사용하여, 약물전달체를 제조하고, 약물의 방출 거동을 살펴 본 결과, β<γ<α순으로 β-키틴이 서방성의 방출 거동을 나타내었다. 이는 분자간 수소결합이 존재하지 않은 β-키틴 분자 내로 약물이 도입되고 또한 이들간의 상호 인력에 의해 약물 방출이 지연되는 것으로 사료되며, 따라서 본 연구에서 제조된 α-β-γ-chitin의 서방성 약물 전달체로써의 가능성을 확인하였다. α-, β-, and y-chitin were isolated from natural resources by chemical method to investigate the crystalline structure of chitin. Their characterization was identified by FT-IR spectrophotometer, solid state CPMAS ^(13)C NMR spectrophotometer, DSC and XRD. A molecular weight (M_(vis)) of α- , β-, and y-chitin were determined by viscometer resulting in 701, 612, and 524 kDa, respectively. And we have prepared the drug carrier according to the α- , β-, and y-chitin and release profile was investigated. At FT-IR spectra, α-, β-chitin showed doublet and singlet at amide I band, respectively, and y-chitin showed intermediate form between α- and β-chitin. From solid state CP/MAS ^(13)C NMR spectra, two signals appeared at around 73 and 75 ppm assigned to C3 and C5 carbon atoms in a-chitin are sharply separated, the signals of C3 and C5 in β-chitin shows singlet at around 74 ppm. In case of y-chitin, two signals show at around 73 and 75 ppm assigned to C3 and C5 carbon atoms. From the X-ray diffraction results, a-chitin was observed four crystalline reflections, shown at 9.6, 19.6, 21.1, and 23.7 by crystalline structure. Also, β-chitin showed two crystalline reflections as indicated at 9.1˚, and 20.3˚ in the crystalline structure spectroscopy. y-chitin with structure of both antiparallel and parallel was close to X-ray diffraction patterns of a-chitin. As the result of DSC, due to difference in structural characteristics, remarkable differences in the exothermic transition for α-, β-, and y-chitin were observed. However, four crystalline reflections observed in the 28 range of 5 - 35˚ were disappeared in case of α-, β- and y-chitin after heating up to 400℃. The exothermic peak in α-, β-, and y-chitin were shows at 330, 220, and 300℃, respectively. The drug carrier were prepared by using α-, β-, and y-chitin, and the behavior of drug release were investigated by PBS 7.4 at 37±0.5℃. As the result, the drug release behavior was order of y-chitin > a-chitin > β-chitin.

국제결혼 이주여성을 위한 효과적인 한국어 지원 정책 방안 연구 : 전북지역 기관별 여성결혼 이민자 지원 프로그램 분석을 중심으로

장미영(Jang Mi-young),소강춘(So Kang-chun),고은미(Ko Eun-mi),이수라(Lee Su-ra) 국어문학회 2008 국어문학 Vol.44 No.-

This study has been advanced with the aims of (1) understanding the situations of Korean education for the international marriage women immigrants in Jeonbuk province. (2) construction the basic data of the efficient support policies on Korean education with a view of the government. (3) devising these efficiently. However, this study was restricted to Jeonbuk province. Because this study was affected with the budget and the number of researchers, so we chose the region which could be taken possibly. Therefore, the targets of this study were institutions and parties which were taking actions, related to the international marriage women immigrants. The ways to research those institutions and parties were the examinations of homepages, telephones, questionnaires and visits. The results arisen from this study show that, first, we could understand the situations of Korean education which have been doing by the institutions and the parties in Jeonbuk province. Second, we could present the efficient methods of Korean education practice. Third, we could propose the alternatives that the government should take actions planning policies for the international marriage women immigrants in Korea.