관상동맥협착 정도 예측을 위한 경동맥 초음파 검사의 유용성

고광일,박병현,오석규,김남호,조정구,정진원 대한노인병학회 2001 대한노인병학회지 Vol.5 No.4

ANATOMICAL EXPANDER와 IMPLANT를 이용한 유방 재건의 경험

고법민,박원진,김재중,하범준,이재승,신명수 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

The use of a tissue expander and implant is the simplest option for breast reconstruction after mastectomy. Use of a round dome-shaped prosthesis and the commonly used one-stage technique with a Becker type prosthesis, however, often produces an undesirable upper pole fullness. To overcome this and to achieve improved aesthetic results, we started using an anatomically-shaped expander and implant, as described by Maxwell, as a two-stage breast reconstruction. We reviewed the results of our 21 reconstructed breasts in 22 patients who were deemed suitable for reconstruction using this technique since January 1995. The most commonly used expander was 350cc (range 350-550cc) and an average of 4.2 inflations were required before replacing the expander with a permanent implant. All the expanders were placed in submuscular pockets and implant volume. The longest follow-up was 36 months. Few complications developed and most patients were satisfied with the results. We found that the anatomically-shaped expander and implant produced better aesthetic results compared to a done-shaped prosthesis.

배양된 간세포 담세관 및 세포질소기관의 미세구조 연구 : Phalloidin과 Cytochalasin D의 영향 Effects of Phalloidin and Cytochalasin D

김진국,양남길,안의태,고정식,박경호,김주원 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.2

간세포 담세관의 주위세포질에는 미세사가 풍부하게 분포하는데, 이 실험에서는 배양 간세포의 미세사형성을 촉진 또는 억제시킨 후에 담세관 및 세포질소기관의 미세구조를 관찰하여 미세사의 형태학적 및 기능적 의의를 연구하였다. Sprague Dawley계 숫흰쥐의 간조직에 IV형 collagenaseㄹ를 처리하여 간세포를 분리한 후 배양하며 phalloidin(미세사 중합 촉진제)이나 cytochalasin D(미세사 중합 억제제)를 배지에 투여하고 전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 1. 분리된 간세포 세포질에는 골지복합체와 소포 및 용해소체들이 세포의 일부분에 분포되어 생체의 간세포와 같이 세포질소기관 분포의 극성을 나타냈다. 2. Phalloidin와 cytochalasin D 투여군이 담세관은 확장되고 미세융모의 소실이 뚜렷하였다. Phalloidin투여군에서는 미세사가 증식되고 외형질층이 두터워져 담세관은 경직된 모습이었고, cytochalasin D투여군에서는 미세사가 감소되고 담세관의 확장은 더욱 심했다. 3. Phalloidin이나 cytochalasin D 투여군 모두에서 간세포 표면에는 세포질이 돌출되었다. Phalloidin투여군에서는 돌출부의 연결부분이 잘록하였고 외형질에 미세사가 증식되어 돌출부와 세포질을 분리시키고 있었다. 반면에 cytochalasin D투여군에서는 외형질층에서 미세사가 뚜렷한 감소되었고 각종 세포질소기관들이 포함된 채로 세포질 일부를 밀고나와 반구형의 돌출부를 이루었고 연결부는 넓었다. 4. 세포질에는 많은 소포와 공포들이 융합되어 세포의 주변부분이나 담세관주위에 세포내공간이 형성되었는데, 이 공간은 세포바깥이나 담세관과 교통되었다. 이상의 결과를 보면, 미세사가 증식 혹은 감소되면 배양간세포의 모습이 변화되며, 담즙의 이동에도 장애가 생겨 담즙정체를 일으킨다. 따라서 미세사는 간세포의 형태유지 및 담즙분비에 중요하게 관여하는 것으로 생각된다. The cytoplasmic microfilaments of hepatocytes are abundant beneath the plasma membrane, especially in the pericanalicular ectoplasm. In this study, the ultrastructural changes of bile canaliculus and cytoplasmic organelles induced by alteration of the microfilaments on the cultured rat hepatocytes were examined. Sprague-Dawley rats(male, about 200gm) were used. The isolated hepatocytes obtained by perfusion of 0.05% collagenase type IV through the portal vein, were cultred in the L-15 medium containing phalloidin(stabilizer of microfilaments) or cytochalasin D(destabilizer of microfilaments) for 10 min, 30 min, 1 hour, 2 hours, 4 hours, 10 hours and 20 hours, respecitvely. The hepatocytes on the cultured dish were fixed in 2.5% glutaradehyde -1.5% paraformaldehyde and 1% osmium tetroside. After alcohol dehydration, the cells were embedded in the araldite mixture. Ultrathin sections were contrasted with uranyl acetate and lead citrate, and observed with JEM100 CX-II electron microscope. The results were as follow: 1. Isolated hepatocytes maintained the typical architectionic relationships of secretory organelles, i.e., Golgi apparatus, vesicles, lysosomes, etc., in the vicinity of bile canalicular region. 2. In the phalloidin or cytochalasin D treated groups, bile canaliculi were dilated and devoid of microvilli. In phalloidin treated group, the pericanalicular ectoplasm containing the microfilaments microfilaments was thicker than that of cytochalasin D treated group. Whereas, the dilation of bile canaliculus was more marked in cytochalasin D group. 3. Both drugs, phalloidin or cytochalasin D, produced the alteration of cell shaper to form cytoplasmic protrusions at the cell surface. In the phalloidin treated group, protusions were pedunculated, and the microfilaments were accumulatd at the narrow neck region. In cytochalasin D treated group, in contrast, no microfilament barrier was seen at the broad base of protrusion which exhibit direct continuity with the internal cytoplasm. 4. Numerous vesicles and vacuoles were formed near the cell surface and perianalicular cytoplasm in the treated groups, and later in culture they fused each other to form large intracellular space. Eventurally. this space was connected to the extracellular space or bile canaliculus. This experiment demonstrated that excessive accumulation or depletion of microfilaments induced by phallyoidein or cytochalasin D altered the cell shape and disturbed the vesicular transport of bile components into bile canaliculi. The results suggest that dysfunction of microfilaments may play an imprtant role in the impairement of canalicular contraction and the integrity of microfilaments is necessary for the billiary secretin as well as for the maintainance of the cell shape of hepatocytes.

Dock Wall에서 Pre-splitting 발파의 적용성에 관한 연구

강추원,고진석,강석곤 조선대학교 동력자원연구소 1997 動力資源硏究所誌 Vol.19 No.1

The contour blasting minimize the strain and the rocks are destroyed to the theoretical projected line by adding or substracting the explosive compound amounts. On pre-splitting blasting, the most important factors are the base rock conditions, accuracy of drilling, kind of explosive compound used, and charge. The first test blast is classified in eight groups, which is done to find more accurate blasting conditions after a variety of blasting methods are used. Through the conditions of charge of the first test blast, after the best blast method is selected the second test blast is performed. The number of holes and hole spacing are corrected on the second test blast, which takes change to reduce construction days and working conditions. In the second test blast charge conditions. Pattern Y is the method for see a cutting plane of 90˚ for the reducing of dock-wall parts.

Li-CICs의 Deintercalation에 따른 구조변이와 열적, 전기적 안정성과의 관계(Ⅱ) : The relation of structural transition, thermal and electrical stability deinter calation of Li-CICs(Ⅱ):For Li-EaGDICs and Li-EGDICs

오원춘,박정오,백대진,고영신 한국분석과학회 1996 분석과학 Vol.9 No.1

가온 가압법에 의하여 합성된 Li-EaGICs, Li-EGlCs를 자발적으로 분해시켜서 이들의 deintercalation 과정에 대하여 X-선 회절분석, 열분해분석 몇 전기비저항값을 측정하여 이들 결과에 대하여 토론하였다. Intercalation에 대한 X-선 회절분석 결과에 의하면, Li-EaGlCs와 Li-EGlCs는 1 stage가 완전하게 형성되지 않았고, 주로 저차 stage가 형성되었다. 또한 deintercalation 결과에 의하면, 4주 이후에는 deintercalation이 멈추었으며 Li-EGDICs는 Li-EaGDlCs보다 층간 잔유 금속을 많이 가지고 있음을 알 수 있다. 열분해분석 결과에 의하면 두 가지 화합물 모두 강한 발열반응을 수반하였으며, 또한 400℃ 이 상에서는 반응이 수반되지 않는 것으로 보아 intercalants들이 완전히 deintercalation되었음을 알 수 있다. 전기 비저항 측정 결과에 의하면 Li-EGDICs는 상대적으로 낮은 비저항값을 가지고 있었으며, Li-EaGDICs는 이상적인 비저항 곡선을 나타내었다. 이들 결과로부터 Li-EaGlCs가 Li-EGICs보다 2차 전지의 양극재료로서 더 좋은 성질을 가지고 있음을 알 수 있다.

加水分解 牛皮粉의 飼料的 價値에 관한 硏究

金大鎭,孟元在,高永杜 東亞大學校 大學院 1984 大學院論文集 Vol.9 No.-

Protein quality of hydrolyzed cattle skin meal was estimated by the pepsin-HCL digestion with various concentration and incubation time, and by chemical score to determine the limiting amino acids. Protein efficiency ratio(PER), net protein ratio(NPR) and net protein utilization(NPU) were also studied with chicks fed semipurified diet with and without supplementation of the limiting amino acids. Broiler chick growth trials were also conducted to determine the supplementation level of hydrolyzed cattle skin meal by replacing with soybean meal and feather meal. The results are summarized as follows: 1. Protein digestibility of hydrolyzed cattle skin meal was 66.31%, 80.69%, 83.72%, 84.65%, and 87.45%, respectively for 4, 8, 12, 16 and 20 hours incubation of 0.2% pepsin-HCl. Protein digestibility maintained above 80% after 8hours incubation and increased by increasing incubation time. 2. Protein digestibilities of hydrolyzed cattle skin meal incubated for 16 hours at 0.2%, 0.1%, 0.05%, 0.025%, and 0.0125% pepsin-HCl solution were 85.10%, 82.08%, 76.18%, 74.67% and 64.82%, respectively. 3. Chemical scores of methionine, isoleucine, threonine, leucine and histidine estimated based on NRC(1977) broiler(03 weeks) feeding standard were 27%, 38%, 44%, 48% and 51%, respectively 4. Growth rate, feed intake and feed conversion were improved by the addition of the limiting amino acids in the hydrolyzed cattle skin meal as compared with hydrolyzed cattle skin meal alone. 5. PER, NPR and NPU were also improved by the addition of the limiting amino acids. 6. Feed intake was not statistically significant upto 5% hydrolyzed cattle skin meal in the diet with was 100% replacement of hydrolyzed cattle skin meal with soybean meal and feather meal. Growth rate and feed conversion were not statistically significant at 3% but were significant 5% hydrolyzed cattle skin meal(p<0.05). These experiments were clearly indicated that hydrolyzed cattle skin meal could supplement in the diet upto 3% when mixed with 2 or 3 other protein sources.

외상성 골낭에 관한 연구

박원길,고재경,김영진,오경란 大韓口腔顎顔面放射線學會 1997 Imaging Science in Dentistry Vol.27 No.2

Traumatic bone cyst is a pathologic cavity that is not lined with epithelium. It is, therefore, not a true cyst. It may be a normal variant rather than a disease process. The etiology of the condition is unknown. This condition is occurred widely ranging ages(2 to 75years), however, most are found during the second decade of life. Radiographically, this condition is radiolucent lesion with well-defined outline, scalloping of superior margins. Cyst enucleation and curettage is the treatment of choice. The authors compared and analyzed the clinicoradiologic features of the five cases of traumatic bone cyst, diagnosed at the Dental college hospital in Chosun University, Kwangju, Korea., The five cases wear shown the followed results; 1. 3cases occurred in second decade of life & no significant sex differences (M:F, 2:3) All cases occurred in mandible. 2. Two patients complained symptoms, but three cases had no symptom with encountering during routine examination. 3. In 3 of 5cases, teeth vitality existed except one tooth and no checking of teeth vitality in two cases. 4. All cases didn't have any accurate trauma history, but one case was in orthodontic treatment, another case was postextraction site area. 5. Radiologically, "scalloping appearance" were evident in all cases; in 3 cases, multilocular tendency & only one case seen intact mandibular canal image. 6. Histologically, all section showed bone trabeculae with blastic activity, 2 cases showed no epithelial lining, and other 2 cases were seen innflammatory cell infiltration in edematous tissue. 7. Surgical intervention (curettage) was that treatment of choi

MTA와 4-META/MMA & TBB레진 혼합 재료의 치근단 미세누출에 관한 연구

김진철,김미리,고현정,양원경 大韓齒科保存學會 2009 Restorative Dentistry & Endodontics Vol.34 No.4

이 연구에서는 Mineral Trioxide Aggregate (MTA)와 4-methacryloxyethyl trimellitate anhydride (4-META)/methyl methacrylate (MMA) & tri-n-butylborane (TBB) 레진을 혼합하여 치근단 역충전 재료로 사용하였을 때의 미세누출을 MTA, 포틀랜드 시멘트와 비교하였다. 발치된 사람 치아의 근관형성 및 충전 후 치근단 절제와 역충전 와동을 형성하였다. MTA, Portland cement, MTA와 4-META/MMA & TBB레진을 혼합한 각각의 재료로 치근단을 역충전한 후 methylene blue dye에 72시간 동안 침적하여 염료가 침투한 길이를 10배 현미경 하에서 측정하였다. MTA와 4-META/MMA & TBB 레진 혼합 충전 군에서는 포틀랜드 시멘트나 MTA 충전군보다 통계적으로 유의하게 적은 미세 누출을 보였으며 , 포틀랜드 시멘트와 MTA 충전군의 미세 누출 정도는 통계적으로 차이가 없었다. 이 실험의 결과로 보아 MTA와 4-META/MMA & TBB레진 혼합 재료는 치근단 역충전시 포틀랜드 시멘트나 MTA에 비해 낮은 초기 미세누출을 보여, 치근단 봉쇄 효과가 우수한 것으로 사료된다. We evaluated in vitro microleakage of Mineral Trioxide Aggregate (MTA) powder with 4-methacry-loxyethyl trimellitate anhydride (4-META) / methyl methacrylate (MMA) & tri-n-butylborane (TBB) resin as a retrograde filling material by using methylene blue dye method. Fifty-two single rooted, extracted teeth were instrumented and obturated with gutta percha and AH plus sealer. The apical 3mm of each root was resected and 3mm deep ultrasonic root end preparation was done. External surface of roots was coated with nail varnish. Prepared teeth were randomly divided into five groups: Negative control: completely covered with nail varnish; Positive control: coated with nail varnish except for apical foramen; Group 1 (retrofilled with Portland cement); Group 2 (retrofilled with MTA) ; Group 3 (retrofilled with MTA powder mixed with 4-META/MMA & TBB resin). Immediately after completion of root-end filling, all specimens were submerged in methylene blue dye for 72 hours in 37℃ incubator. The roots were longitudinally sectioned and measured for extent of dye penetration by three different examiners under microscope (×10). The results were statistically analyzed using one way ANOVA and Turkey's HSD test. No leakage was evident in negative control and complete leakage in positive control group. Group 3 showed significantly less leakage than group 1 and 2 (p < 0.01). There was no significant difference between group 1 and 2 (p > 0.01). It was concluded that MTA powder with 4-META/MMA & TBB resin was excellent in reducing initial apical microleakage. 〔J Kor Acad Cons Dent 34(4):371-376, 2009〕

한국 약용식물부터 폴리폐놀류군의 분리 및 기능성화장품소제로서의 응용

배두경,김성기,고대원,이창언,이진태,안봉전 경산대학교 생명자원개발연구소 2001 생명자원과 산업 Vol.5 No.-

For develope the functional cosmetic material. we extracted the Korean persimmon leaves in 80% ethanol solution and isolated fraction I, II, III, to use Sephadex LH-20 column. Their polyphenol quantity was 20%, 70%, 82.8% individually and fraction II, III had polyphenol which included a many flavan-3-ol skeleton structure. We investigated various biological activity such a tyrosinase, xanthin oxidase inhibition, SOD-like acivity and DPPH. Inhibitory effect of tyrosinase was inhibited 51.7%, 74.7% at 50 ppm, 500 ppm of the fraction III and SOD-like activity increased quickly at 500ppm of the fraction II, III. DPPH effect had 80% electron donate ability at 50 ppm all of the fraction and xanthin oxidase was more inhibited 80% at 50 ppm of fraction II, III. These results showed possibility that polyphenols of the Korean persimmon leaves become the functional cosmetic ingredient

KAL기 추락에 의한 대량재해의 개인식별

강신몽,이원태,고영창,최상규,김윤희,이홍석,서재관,윤중진,이혜경,최득린,김종열,윤창육,변명식,이장홍 大韓法醫學會 1991 대한법의학회지 Vol.15 No.2

Individual identification is an important part in medicolegal field especially in mass disaster. At July, 27, 1989, KAL KE-803 was crashed on landing at Tripoli International Airport, Liba. The plane was caught in fire and sixty eight Koreans were sacrified. The majority of victims were severely charred and injured. The authors examed all dead bodies and successfully identified all the cases through visual, anthropological, odontological, radiological and pathological methods including fingerprint and blood typing.