A Wireless Intraocular Pressure Sensor with Variable Inductance Using a Ferrite Material

Kang, Byungjoo,Hwang, Hoyong,Lee, Soo Hyun,Kang, Ji Yoon,Park, Joung-Hu,Seo, Chulhun,Park, Changkun The Institute of Electronics and Information Engin 2013 Journal of semiconductor technology and science Vol.13 No.4

A wireless intraocular (IOP) pressure sensor based on micro electro mechanical system (MEMS) technology is proposed. The proposed IOP sensor uses variable inductance according to the external pressure. The proposed sensor is composed of two flexible membranes: a ferrite bottom part, an inductor, and a capacitor. The inductance of the sensor varies according to the external pressure. The resonance frequency of the sensor is also varied, and this frequency is detected using an external coil. The external coil is designed with an FR-4 printed circuit board. The feasibility of the proposed sensor structure using variable inductance to detect the external pressure is successfully demonstrated.

마이크로파 방식 무선전력전송용 low-profile 고이득 배열 안테나 설계

강은정(Eunjung Kang),서철헌(Chulhun Seo),추호성(Hosung Choo) 대한전기학회 2021 대한전기학회 학술대회 논문집 Vol.2021 No.7

[Evaluation of a multiplex PCR kit used for detecting y chromosome microdeletions.]

Park, Mi Young,Kang, Hye Min,Hwang, Sang Hyun,Park, Hyun Jun,Park, Nam Cheol,Choi, Kyung Un,Kim, Hyung Hoi,Chang, Chulhun L,Lee, Eun Yup 대한진단검사의학회 2010 Annals of Laboratory Medicine Vol.30 No.4

<P>BACKGROUND: In addition to Klinefelter's syndrome, microdeletion of Yq is the most common genetic cause of male infertility; 15% of azoospermic or 5-10% of oligozoospermic males have Yq deletions. We evaluated a Yq microdeletion kit (LG Life Sciences, Korea) for identifying microdeletions in the azoospermic factor (AZF) regions of the Yq. METHODS: The kit was designed to amplify 3 regions of the AZF gene (AZFa, AZFb, and AZFc) using 15 sequence-tagged sites. We evaluated the preclinical performance of the kit. For clinical validation, 58 patients including 25 idiopathic azoospermic or oligozoospermic patients were examined. RESULTS: We observed clear bands on electrophoresis of DNA, up to a DNA concentration of 3.12 ng/microL; the known microdeletion regions of all 6 reference cell-lines (Coriell, USA) were accurately detected and no false positive/negative results showed with normal female (n=11) and fertile male (n=15) specimens. This kit could identify the same microdeletions in the common regions, similar to another commercial kit. Among the 58 male infertile patients, 7 (12.1%) had microdeletions of the Yq. Among the idiopathic azoospermic (n=22) and oligozoospermic (n=3) patients, 3 (12.0%) had microdeletions. Further, 2 of 21 varicocele patients (9.5%), 1 of 4 patients with testicular failure, and 1 patient with a 45,X/46,XY mosaic had microdeletions. CONCLUSIONS: The kit was effective for detecting microdeletions of the Yq. We identified microdeletions in 12% of the infertile patients. This Y chromosome microdeletion detection kit is useful for screening Yq microdeletions in infertile patients.</P>

Hydrogen sulfide‐activatable fluorescence turn‐on azide‐containing naphthalimide derivative

Song Yi Yoo,Chulhun Kang,Min Hee Lee,Lavanya Gopala 대한화학회 2022 Bulletin of the Korean Chemical Society Vol.43 No.11

Hydrogen sulfide (H2S) acts as a crucial biological and environmentalcomponent in a variety of processes. Abnormal levels of H2S endogenouslygenerated in human body are associated with various diseases. Meanwhile,H2S gas leaked from various manufacturing environments has a fatal effectagainst the human body. For this reason, H2S has been considered as a potentialbiomarker for the diagnosis and treatment of various human diseases, and as atoxic gas that should be detected when leaked to the workplace for the safety ofworkers. In this study, we synthesized an azide-containing naphthalimide derivativethat can play as a fluorescence turn-on probe for H2S.

[Antimicrobial Susceptibility Patterns of Legionella isolates in the Environment and in Patients.]

Choi, Go Eun,Kang, Jeong Eun,Lee, Eun Yup,Chang, Chulhun L,Tateda, Kazuhiro,Yamaguchi, Keizo,Kim, Kyeong Hee,Kim, Jeong Man 대한진단검사의학회 2010 Annals of Laboratory Medicine Vol.30 No.1

<P>BACKGROUND: Antimicrobial susceptibility of Legionella spp. has rarely been studied in Korea. Therefore, we aimed to determine the susceptibility of Legionella spp. to various antibiotics. METHODS: We assessed the antimicrobial susceptibility of 66 environmental and clinical Legionella isolates collected between January 2001 and December 2008 from Korea and Japan. The minimum inhibitory concentrations (MICs) of 6 antibiotics, namely, azithromycin, ciprofloxacin, clarithromycin, clindamycin, gatifloxacin, and gemifloxacin were determined by the broth microdilution method using buffered starch yeast extract broth. RESULTS: The MIC ranges of the 6 antibiotics used against the Legionella isolates were as follows: 0.004-0.062 microg/mL (azithromycin), 0.002-0.5 microg/mL (ciprofloxacin), 0.004-0.5 microg/mL (clarithromycin), 0.12-4 microg/mL (clindamycin), 0.002-0.12 microg/mL (gatifloxacin), and 0.008-1 microg/mL (gemifloxacin). CONCLUSIONS: Legionella spp. isolates from Korea and Japan were most susceptible to gatifloxacin. Azithromycin, clarithromycin, ciprofloxacin, and gemifloxacin were also effective for treating legionellosis.</P>

Biotin-guided anticancer drug delivery with acidity-triggered drug release

Park, Soyeon,Kim, Eunjin,Kim, Won Young,Kang, Chulhun,Kim, Jong Seung The Royal Society of Chemistry 2015 Chemical communications Vol.51 No.45

<P>A novel biotin-guided anticancer drug delivery system, prodrug <B>9</B>, consisting of biotin, nitrobenzene, and doxorubicin, with acid-triggered drug releasing capability was synthesized. Its cellular uptake and anticancer activity are selective to the HepG2 cell line over the WI-38 cell line, as revealed by fluorescence confocal microscopic experiments and MTT assay.</P> <P>Graphic Abstract</P><P>A novel biotin-guided anticancer drug delivery system, prodrug <B>9</B>, consisting of biotin, nitrobenzene, and doxorubicin, with acid-triggered drug releasing capability was synthesized. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c5cc03003j'> </P>

Hydrophobic Aminostyryl Quinolinium Dyes as New Fluorescent Stains for Proteins in Sodium Dodecyl Sulfate-polyacrylamide Gel

Sooyeon Hong,Seung Soo Yoon*,Chulhun Kang,Myungkoo Suh* 대한화학회 2004 Bulletin of the Korean Chemical Society Vol.25 No.3

Zingiber officinale Protects HaCaT cells and C57BL/6 Mice from Ultraviolet B-Induced Inflammation

Gui-Hee Guahk,Sang Keun Ha,정혁상,Chulhun Kang,김창현,Yoon-Bum Kim,Sun Yeou Kim 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.3

Ultraviolet (UV) light is a physical carcinogen, and UV irradiation from sunlight has negative effects on human skin. UVB-induced inflammation is linked to excessive induction of various inflammatory cytokines/chemokines in many types of cells, including keratinocytes. The purpose of this study was to investigate the anti-inflammatory effect of water extract of Zingiber officinale, gingerol, and shogaol on UVB-induced skin damage in the human keratinocyte cell line HaCaT and C57BL/6 mice. To test for an effective compound to protect against inflammation in UV-damaged skin, we prepared a water extract of ginger rhizomes and examined the effects of Z. officinale, gingerol, and shogaol on cell viability and cytokine/chemokine production in UV-irradiated HaCaT cells. We also investigated the in vivo relevance of these findings in C57BL/6 mice using hematoxylin and eosin staining and cytokine measurements. A water extract of Z. officinale, gingerol, and shogaol inhibited production of cytokines in UVB-irradiated HaCaT cells effectively. Treatment with Z. officinale attenuated UVB-induced hyperplasia, infiltration of leukocytes, and dilation of blood vessels in the dermis of mice. Z. officinale, gingerol, and shogaol show potential as anti-inflammatory agents to protect skin against UVB irradiation damage.

Shedding light on tau protein aggregation: the progress in developing highly selective fluorophores

Verwilst, Peter,Kim, Hyeong Seok,Kim, Soobin,Kang, Chulhun,Kim, Jong Seung The Royal Society of Chemistry 2018 Chemical Society reviews Vol.47 No.7

<P>Historically, in Alzheimer's disease research, a lot of attention has been paid to the development of highly selective fluorophores for beta amyloid plaques. With a shift in the understanding of the disease and the importance of a network of cross-talk interactions, the development of small-molecule fluorescent dyes with high selectivity for (hyperphosphorylated) tau protein aggregates in neurofibrillary tangles has been gaining increasing attention. Fluorescent dyes for the selective labelling of tau aggregates in histological AD brain sections have been described, spanning the entire visible range of the electromagnetic spectrum. Despite the relatively early stages of the development of the field, a large diversity in probe architectures has been reported. Importantly, a handful of near-infrared-emissive dyes have been described as well, and some of these have exhibited good pharmacological profiles, with a significant blood-brain-barrier permeability, and a demonstrated ability to label tau tangles<I>in vivo</I>in small-animal models of Alzheimer's disease and other tauopathies. The developments summarized in the current work are expected to aid the unravelling of the diverse set of players in the etiology of Alzheimer's disease. In this tutorial review, we seek to provide the reader with an overview of the most important recent developments and hope to provide some guidelines for the design of future probes.</P>

A coumarin-naphthalimide hybrid as a dual emissive fluorescent probe for hNQO1

Park, Sun Young,Won, Miae,Kang, Chulhun,Kim, Jong Seung,Lee, Min Hee Elsevier 2019 Dyes and pigments Vol.164 No.-

<P><B>Abstract</B></P> <P>hNQO1 is a promising target for cancer diagnosis and treatment. Here, we developed a coumarin-naphthalimide hybrid (<B>1</B>) as a dual emissive fluorescent probe that enhances the emission through hNQO1-mediated cleavage of its trimethyl lockquinone moiety. Probe <B>1</B> can be used for a real-time monitoring of hNQO1 activity in living cells.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A coumarin-naphthalimide hybrid (<B>1</B>) as a hNQO1 probe was synthesized. </LI> <LI> The probe exhibited a selective fluorescence change for hNQO1. </LI> <LI> Probe showed a high specificity constant (7.66 ± 0.02) x 10<SUP>4</SUP> M<SUP>−1</SUP>s<SUP>−1</SUP> for hNQO1. </LI> <LI> Probe displayed a dual fluorescence image in hNQO1-positive A549 cells. </LI> <LI> Probe can be used to diagnose the diseases associated with hNQO1. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>