축구선수의 Detraining과 Retraining이 혈청지질 및 호르몬농도에 미치는 영향

정정화,박재현,채종훈,성혜련,황지인,윤미숙,노금선,윤종관,윤영학,노순덕,정경숙,박일규,김은희,박현태,박상갑 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1

The purpose of this study was to investigate the effects of detraining and retraining on serum lipid and hormones in soccer players. Subjects were seven male high-school soccer players. V˙O_2max was determined for each subjects by administering a treadmill test(initial speed: 90m/min, grade: 5%, increasing speed per 3 min: 30m/min). Serum lipid(T-C, TG, HDL-C LDL-C) and hormones(epinephrine, norepinephrine, growth hormones, cortisol) were assayed pre and post detraining in 10, 20, 30 days after retraining. The repeated ANOVA was used to determine significant differences. The 0.05 level of significance was as critical level for the study. The results of the study were as follows: 1. V˙O_2max(ml/min) were 3576.3±204.2ml/min pre detraining, 3234.1±198.9 ml/min post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/min) were 3601.4±170.9 ml/min. There were significantly(p<.05) increased in retraining periods. 2. V˙O_2max(ml/kg/min) were significantly(p<.05) decreased from 62.3±2.9 ml/kg/min to 55.9±4.7 ml/kg/min in detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/kg/min) were 62.4±3.4ml/kg/min, 62.7±2.3ml.kg/min, 67.3±7.2ml/kg/min respectively. There were significantly(p<.05) increased in retraining periods. 3. T-C were significantly (p<.05) increased from 166.6±8.5mg/dl to 175.3±10.3 mg/dl in detraining. In 10, 20, 30 days after retraining, T-C were 160.1± 3.2mg/dl, 156.7±3.7mg/dl, 140.3±9.0mg/dl. There were significantly(p<.05) decreased in retraining periods. 4. HDL-C were 61.4±6.6mg/di pre detraining, 5.3±6.6mg/dl post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, HDL-C were 56.9±7.1mg/dl, 56.4±9.2mg/dl, 57.7±9.1mg/dl respectively. There were no significant difference in retraining periods. 5. The hormones(epinephrine. norepinephrine, growth hormone, cortisol) were changed as same patterns. Epinephrine were 26.0±7.0[g/ml pre detraining, 24.6±3.2pg/ml post detraining. In 10, 20, 30 days after retraining, epinephrine were 26.9±5.6pg/ml, 30.6±6.2pg/ml, 29.4±5.6pg/ml respectively. There were no significant difference in retraining periods. In conclusion, HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were decreased, T-C, LDL-C and TG were increased in detraining. But HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were increased, T-C, LDL-C and TG were decreased in retraining.

Insulin resistance mediates high-fat diet-induced pulmonary fibrosis and airway hyperresponsiveness through the TGF-β1 pathway

Park, Yoon Hee,Oh, Eun Yi,Han, Heejae,Yang, Misuk,Park, Hye Jung,Park, Kyung Hee,Lee, Jae-Hyun,Park, Jung-Won Nature Publishing Group UK 2019 Experimental and molecular medicine Vol.51 No.5

<▼1><P>Prior studies have reported the presence of lung fibrosis and enhanced airway hyperresponsiveness (AHR) in mice with high-fat-diet (HFD)-induced obesity. This study evaluated the role of TGF-β1 in HFD-induced AHR and lung fibrosis in a murine model. We generated HFD-induced obesity mice and performed glucose and insulin tolerance tests. HFD mice with or without ovalbumin sensitization and challenge were also treated with an anti-TGF-β1 neutralizing antibody. AHR to methacholine, inflammatory cells in the bronchoalveolar lavage fluid (BALF), and histological features were evaluated. Insulin was intranasally administered to normal diet (ND) mice, and in vitro insulin stimulation of BEAS-2b cells was performed. HFD-induced obesity mice had increased insulin resistance, enhanced AHR, peribronchial and perivascular fibrosis, and increased numbers of macrophages in the BALF. However, they did not have meaningful eosinophilic or neutrophilic inflammation in the lungs compared with ND mice. The HFD enhanced TGF-β1 expression in the bronchial epithelium, but we found no differences in the expression of interleukin (IL)−4 or IL-5 in lung homogenates. Administration of the anti-TGF-β1 antibody attenuated HFD-induced AHR and lung fibrosis. It also attenuated goblet cell hyperplasia, but did not affect the AHR and inflammatory cell infiltration induced by OVA challenge. The intranasal administration of insulin enhanced TGF-β1 expression in the bronchial epithelium and lung fibrosis. Stimulating BEAS-2b cells with insulin also increased TGF-β1 production by 24 h. We concluded that HFD-induced obesity-associated insulin resistance enhances TGF-β1 expression in the bronchial epithelium, which may play an important role in the development of lung fibrosis and AHR in obesity.</P></▼1><▼2><P><B>Obesity: A trigger for asthma onset</B></P><P>Insulin resistance may be an important causative factor underlying the increased risk of asthma and other respiratory issues in obese individuals. Obesity doubles the likelihood of developing asthma, with symptoms that are more difficult to control than in non-obese patients. The connection between these conditions is poorly understood, but researchers led by Jung-Won Park, Yonsei University College of Medicine, Seoul, South Korea, have identified a potential mechanism. They demonstrated that a signaling molecule called TGF-β1 contributes to airway sensitivity and tissue scarring in a mouse model of diet-induced obesity. Subsequent experiments showed that treatment with insulin also gives rise to increased TGF-β1 production in the mouse lung. Since insulin resistance is a common feature of obesity, resulting in abnormally high levels of circulating insulin, this could also account for the increased risk of respiratory problems.</P></▼2>

노인의 영적 간호요구

최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.

재래의류시장의 e-business 적용 전략 연구

윤문길,정대영,이신수,이혜영 한국경영과학회 2000 한국경영과학회 학술대회논문집 Vol.- No.2

Dongdaemoon clothes market can make traditional commerce to improve problems which happen to traditional commerce and satisfy between customers and salers through combination among Electronic Commerce which is growing up in 21 centuries place innovation This thesis is focusing on suggesting strategies which practice to let Dongdaemoon clothes market customers and wholesalers, retailers to use Electronic Commerce as strategic skills by analyzing core successful factors for adopting Electronic Commerce in Dongdaemoon clothes market. Adopting Electronic Commerce in Dongdaemoon clothes market when the customer make a reservation and the salers provide the customer with discounting service, the customer was willing to but it. Internet service categories which affect customer's satisfaction are providing lots of product information. This thesis shows providing information made the customer to increase customers's satisfaction degree and buying intention. Also convenience of product research, and reliability in transaction process can enable the customer to increase transaction reliability. These factors are very important in Electronic Commerce. In addition, factors which show customer's suggestion and incovenience by using best seller information and discounting service board when they buy some items in Dongdaemoon clothes market affect the customer satisfaction degree and satisfaction degress of providing information. However, this thesis is analyzed that reliability of transaction process doesn't affect all successful factors such as product quality, size, online payment system, price reliability.

Effect of p-Phenylenediamine Application to Rat Skin on the Liver Oxygen Free Radical Systems

윤종국,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2003 Journal of biomedical laboratory sciences Vol.9 No.2

To evaluate the influence of hepatic oxygen free radical systems on liver injury by topical p-phenylenediamine (PPD) application on rat skin. PPD (25mg/16.5㎠) was topically applied to the abdominal region 5 times every other day and sacrificed. By PPD treatment, increasing rate of liver weight/body weight(%), serum activities of alanine aminotransferase and aspartate aminotransferase and decreasing rate of microsomal glucose-6-phosphatase activity were higher in the rats fed tungstate supplemented diet than those fed a standard diet. These findings indicate that group fed tungstate supplemented diet have more severe liver injury compared with group fed standard diet on topical PPD application. However, the activities of oxygen free radical generating enzymes such as xanthine oxidase (XO) and cytochrome P450 dependent aniline hydroxylase and those of oxygen free radical scavenging enzymes were not found to be different between these two animal groups. In the present study, a novel monitoring method to detect the generating of oxygen free radicals in liver extract was devised. Throughout this method, the oxidized PPD produced by oxygen free radicals was determined colorimetrically. The increasing rate of PPD oxidation by liver homogenate was higher in tungstate fed animals than in standard diet fed ones. Among the fractionations of liver extract, the mitochondria1 and postmitochondrial fractions in the liver extract of tungstate fed animals led to a higher availability of PPD oxidation by PPD treatment compared with standard diet fed ones. In conclusion, these results suggest that an enhanced liver injury in tungstate fed animals treated with PPD may be due to oxygen free radicals produced in other systems except oxygen free radicals generating from cytosolic XO system. Especially, oxidative availability by PPD can be used for oxygen free radical detection in some tissue.

디지털 기술의 건강보험 보장방법에 대한 고찰: 5개 국가 중심

엄혜은,김지혜,한승진,최연미,최윤정 건강보험심사평가원 2022 HIRA RESEARCH Vol.2 No.1

옥수수유 및 정어리유 섭취가 발암물질을 투여한 흰쥐에서의 혈청 중성지질 및 콜레스테롤 함량에 미치는 영향

윤혜진,김정희 서울여자대학교 자연과학연구소 1995 자연과학연구논문집 Vol.6 No.-

지방산의 종류는 암의 생성과정 뿐만 아니라 암화과정의 억제 및 촉진에 다른 영향을 미치고 있음이 알려져왔다. 그러나 아직도 그 기전에 대해서는 확실히 밝혀지고 있지 않은 실정이다. 그러므로 본 연구에서는 n-3 지방산인 정어리유 섭취시에는 n-6 지방산인 옥수수유와는 다른 영향을 미칠 것이라고 생각되므로 발암물질을 투여하여 12주간 사육하는 동안에 4, 6, 8, 12주에 각각 sampling을 하여 혈청 지질조성이 어떻게 영향을 받는지를 연구하였다. 1. 체중은 옥수수유군 보다 정어리유군에서 높은 경향을 보였고 발암물질 투여시에는 감소되는 경향을 보였으며 특히 발암물질 투여시 간의 비대화와 섬유화가 대부분의 섬유화가 대부분의 실험쥐에서 관찰 되었다. 2. 혈청의 중성지방 함량은 유지의 종류와 발암물질의 투여 여부에 따른 현저한 차이를 보였는데, 정어리유군에서 옥수수유군 보다 유의성있게 낮았으면 발암물질 투여군에서 낮아졌다. 분석시기에 따른 변화는 4주에서는 정어리유군에서 옥수수유군 보다 유의성 있게 낮았고 발암물질 투여에 의하여 낮아지는 경향을 보였으며, 6주군에는 군간의 유의적인 차이를 보이지 않았고, 8주에는 SO군에서 감소되었고 CO-CAR군에서는 유의적으로 증가되었으며, 12주에는 CO군만이 유의성 있게 증가되었다. 3. 혈청의 콜레스테롤 함량은 정어리유군에서 유의성 있게 낮았으며 발암물질 투여에 의한 차이는 6주와 8주에는 발암물질 투여군에서 낮아졌다가 12주에는 반대로 높아지는 경향을 보였다. 분석시기에 따른 변화는 발암물질을 투여하지 않은 군은 6주에서 증가하고 12주까지 계속 감소되는 경향을 보이는데 반해서 발암물질을 투여한 군은 주별로 큰 차이를 보이지 않아 12주에는 발암물질을 투여하지 않은 군에 비하여 오히려 높은 결과를 보여 주었다. 4. HDL-cholesterol의 함량은 옥수수군에 비하여 정어리유군에서 유의성 있게 낮았으나 발암물질 투여에 의한 효과는 6주와 8주에는 발암물질 투여군에서 유의성있게 낮아졌으나 4주와 12주에는 유의적인 차이를 볼 수 없었다. 시기에 따른 변화는 4주에는 정어리유군에서 낮았고, 6주와 8주에는 CO군에서 증가되었으며, 12주에는 발암물질 투여구에서 증가하는 경향을 보여 SO군에서 유의성 있게 낮았다. 이상의 결과를 종합하면 EPA나 DHA를 많이 함유한 정어리유의 섭취는 혈청 cholesterol양이나 중성지질의 양을 감소시킴에 따라 심장순환계 질환의 예방 식이로 바람직함을 알 수 있었고 아울러 발암물질 투여시 혈청 지질대사에 옥수수유와는 다른 영향을 미치는 것을 알 수 있었으므로 암예방에 좋은 효과를 기대할 수 있을 것으로 사료된다. The present study examined effects of sardine oil rich in n-3 fatty acids and corn oil rich in n-6 fatty acides on serum lipid levels in chemically induced hepatocarcinogenesis system. Weanling male Sprague-Dawley rats were fed either 15% corn oil or sardine oil. Two weeks after feeding, the rats were given a single intraperitoneal injection of diethylnitrosamine (200mg/kg body weight). From 4 week, rats were given 0.02% 2-acetylaminofluorene(2-AAF) in diet for 4 weeks. At 6 week, 0.05% phenobarbital(PB) was added to the diets until the end of experiment. Samplings were conducted at 4,6,8, and 12 week. Body weight of sardine oil (SO) group tends to be higher than that of corn oil (CO) group at all sacrifice time although the effect was not significant. However the body weight was decreased by carcinogen treatment. Serum triglyceride (TG) level of SO group was lower than that of CO group at 4,8 and 12 week. By carcinogen treatment, the serum TG level was significantly increased at 8 week but decreased at 12 week. Such effect was greater in CO feeding group. Serum total cholesterol level of SO group was also lower than that of CO group at all weeks. The change pattern for serum total cholesterol level by DEN, 2-AAF and PB treatment was similar irrespective of dietary oil. Interestingly at 8 week serum total cholesterol level was significantly decreased by carcinogen treatment in CO feeding group while not significantly decreased in SO feeding group. However at 12 week serum total cholesterol level was significantly increased by carcinogen treatment. Serum HDL-cholesterol levels of So group were lower than that of CO group. Serum HDL-cholesterol level of carcinogen untreated group was increased up to 6 week, and then remain constant after that. But HDL-cholesterol level of carcinogen treated group was lower than that of carcinogen untreated group at both 6 and 8 week and then increased at 12 week. Overall results indicate that fish oil not only has a lowering effect on both serum cholesterol and triglyceride level but also differs from corn oil in terms of the changes in serum lipid metabolism during hepatocarcinogenesis.

대학생의 생활스포츠 참여와 대학생활 실태에 관한 연구

윤오남,정혜윤,김보정 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

This study examines the relationship between undergraduates' participation in life sports and their adaptation to campus life and focuses on examining the difference in their adaptation to campus life depending on set period and frequency of participation in life sports and their health on the assumption that their satisfaction with life by their participation in life sports have an affirmative influence on social life as well as campus life. First, adaptation of participants in life sports to campus life was higher in male undergraduates than in female undergraduates according to sex. Second, there was a significant difference in their adaptation to campus life according to frequency of participation in life sports. Third, there was a significant difference in their adaptation to campus life according to period of participation in life sports. Fourth, there was a significant difference in their adaptation to campus life according to intensity of participation in life sports. First, for inducing undergraduates to participate in life sports, attempts to invite them to participate in life sports should be made through expansion and improvement of life sports facilitie And to extend their participation in life sports, components of life sports including life sports facilities, programs and coaches should be extended and improved quantitatively. Motivation of undergraduates to take part in life sports should be encouraged and its improvements are urgently required.

입원전담전문의 진료지원시스템의 설계 및 도입과 병원정보시스템과의 통합 방안

정윤빈,신형식,우가희,정혜경,정진경,고미영,김진수,김소원,이강영 건강보험심사평가원 2021 HIRA RESEARCH Vol.1 No.2

유전성대사질환의 유전자치료

윤혜란,정성철 덕성여자대학교 약학연구소 2006 藥學論文誌 Vol.17 No.-

Inherited metabolic disease, inborn errors of metabolism is a group of genetic diseases defect in the metabolic pathways. Alterations in metabolic pathways are usually caused by defect of enzymes in the process. Generally, inherited metabolic diseases show heterogenous clinical features and symptoms may differ between siblings and families. Excess accumulation of precusors and their toxic derivatives in target tissues result in organ failures, such as central nervous system involvement and hepato-splenomegaly. Therapeutic starategies for inherited metabolic disease, including bone marrow transplantation, enzyme replacement therapy, and diet restriction, have been developed with some positive outcomes. However, these treatments could not be an ultimate therapeutic syarategy, their effects are temporary and give a burden to patients and their families economically and psychologically. Gene therapy can be a best choice for the treatment of inherited metabolic disease. Gene therapy involves the placement of missing genes into particular tissues by the harnessing of suitable vectors (originally these were animal viruses such as adenovirus). For example, gene therapy in the rat for diabetes has succeeded by liver-production of insulin (using genes obtained from pancreatic Islets of Langerhans cells). Many inborn errors of metabolism could be treated in this way: examples may include 100 haemoglobinopathies (such as sickle cell anaemia), phenylketonuria (PKU) etc. Here we present examples of gene therapy progress for Gaucher disease, Fabry disease, PKU, and MPS 1 (Hurlers syndrome) caused by lack of tissue-production of a particular enzyme (in its catalytically-active conformation).