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AH-66 Hepatoma 세포의 Histone에 관한 연구
최종오,이동욱,이희성 중앙대학교 의과대학 의과학연구소 1985 中央醫大誌 Vol.10 No.2
In attempt to investigate histone fractions of tumor cells, histone fractions HI, H2a, H2b, H3 and H4 were prepared from isolated nuclei of AH-66 hepatoma cells and histone fractions were characterized by amino acid analysis and by polyacrylamide disc gel electrophoresis. The results obtained were as follows; 1, The five histone fractions found in most tissues were also present in the AH-66 hepatoma cell histories. 2. The yield of whole histone was 6.46mg per g of packed cells. 3. The yield of DNA was 14.78mg per g packed cells. Consequently the DNA to histone ratio was 1:0.44. 4. The relative amounts of five fractions, i.e., H1, H2a, H2b, H3 and H4 were 15.64% 34.83%, 21,21%, 11.76% and 16.56%, respectively. The data presented in this report show that there is a considerable variation in the relative amounts of histone fractions as compared to those of calf thymus. 5. The electrophoretic mobility of individual histone fractions gave almost similar patterns to those of corresponding fractions of calf thymus. 6. Amino acid analysis of the individual histone fractions showed that the over-all compositions were similar but not identical to those of corresponding fractions from calf thymus and other tumor cells. 7. Histone fractions HI, H2b. H3 and H4 were found to contain detectable amounts of ε-N-monomethyllysine. Usually the methylated derivatives of histidine have been reported in histone Hi fraction of Sarcoma 180 ascites tumor cells and Walker 256 carcinosarcoma. However, the methylated histidine residue was not found in all histone fractions from AH-66 hepatoma cells. 8. The evidence was presented that AH-66 hepatoma cells contained five major histone fractions which were homologous to corresponding five fractions of calf thymus.
Stuides on Histone ( Ⅵ ) : Histones from Normal and Myelocytic Leukemia Leucocytes
이근배,이희성,전재수,최재득 생화학분자생물학회 1980 BMB Reports Vol.8 No.1
The five major fractions of histone from normal and myelocytic leukemia leucocytes were prepared by the procedure of Johns. Electrophoretic behaviors on acrylamide gels and amino acid composition of various histone fractions from normal and leukema leucocytes: were compared:: The results obtained were as follows 1. The yields of whole hisfome isolated from normal and : inyelocytic leukemia leucocytes recovered were 37.08㎎ and 58.64㎎ per lg packed cells, respectively. 2. A marked increase in the amount of fraction KAS (f2b) from leukemia leucocytes was observed. These events were apparently correlated with a parallel decrease in the fractions KAP (f1) and ARE (f3). The .amount of fraction fZa (GRK+LAK) was almost to those of corresponding fraction of normal leucocytes. 3. Amino acid analysis showed the fraction ARE (f3) from leukemia cells contained markedly greater amounts of e-N-monomethyllysine and e-N-dimethyllysine than that from the normal cells.
身體 不自由 學生들의 體育授業에 對한 實態와 指導策에 關한 硏究
李商鍾,李惠姬,姜熙成 全北大學校 1980 論文集 Vol.22 No.-
The followings are the result of research on the actual condition of disabled students. To get the result, I met 122 middle school boys and 102 middle school girls and 103 high school boys and 52 high school girls. 1. They became the disabled man at the age of 4~6. They became the disabled man due to disease and accident. 2. Occupation of their protectors were mostly business and agriculture. 3. Most of the boys liked to play soccer and baseball and school girls liked volley ball. 4. They wanted to play pingpong because they thought pingpong was fit for their physical condition. 5. It was found that teachers of the physical education were not concerned about the disabled students. 6. They had study-hours for themselves and took a rest instead of outdoor sports because they didn't like to show their disabled bodies. 7. They felt unwell that they had been excluded at the school-hour of physical training and they wanted to take part in the study of physical education. 8. They were discontented with marks in physical examination and they wanted to have examination through another special ways. The guiding methods of disabled students in the instruction of physical education. 1. Physical educaters make them enjoy their pfavorite sports. 2. Physical educaters have to have much concern for them. 3. Physical educaters make them enjoy make kind of sports activity like another gerneral students because they wanted. 4. Their marks in physical education must be validly valued according to the special ways.
Ferrous Oxalate Dihydrate부터 다공질 철분말의 제조 및 소결성
이상엽,이동희,이재영 연세대학교 산업기술연구소 1989 논문집 Vol.21 No.2
A systematic study on the production of fine iron powders containing numerous micro-pores by reduction of ferrous oxalate dihydrate and also on the sintering behavior of their powder compacts to impart uniformly dispersed fine pores in the sintered mass has been carried out. Very fine porous iron powders(B.E.T. size≤0.4㎛, specific surface area 15m²/g) produced from ferrous oxalate dihydrate were resulted from the evolution of gases during reduction process. Powders reduced around 400℃ for 30∼60 min, showed the good sinterability due to a kind of the activated sintering even at low sintering temperature (700-800℃), mainly because of lager surface area and unreacted inclusions.
이병영,채하석,전재식,오희필,김도성 대한방사선 방어학회 1989 방사선방어학회지 Vol.14 No.2
옥외 라돈이 호흡기관에 주는 선량을 측정·평가할 목적으로 CR-39 비적검출기를 내장한 라돈컵을 사용하여 대기중 라돈농도를 측정하였다. 직접형 검출기 및 개방 컵과 필터 컵의 구조를 갖는 CR-39비적검출기에 대한 라돈검출인자는 공기중의 농도가 잘 알려진 표준라돈 조사시설에서 이들 검출기와 라돈컵을 일정기간 조사하여 결정하였다. CR-39를 70°c, 30% NaOH용액으로 220분간 화학부식하였을 때 직접형 거출기와 개방 컵, 필터 컵에 대한 라돈검출인자는 각각 0.273, 0.0813, 0.0371tr mm-2/37 Bqm-3·d)였다. 또한 1988년 5월에서 1989년 3월까지 대전(충남대학교)에서 측정한 대기중의 라돈농도는 개방 컵에 의한 결과는 27.4 - 135.8Bq/m3(0.74 - 3.67 pCi/l)로서 연평균 73.3 Bq/m3(1.98 pCi/l)이었으며, 필터 컵에 의한 결과는 16.7 - 143.9 Bq/m3(0.45 - 3.89 pCi/l)로 연평균 68.5 Bq/m3(1.85 pCi/)이었다. 측정한 옥외 대기중이 라돈농도와 부위별 폐선량모형으로부터 산출한 ICRP표준인의 호흡기관에 대한 실효 선량당량률은 약 520 nSv/h로 평가되었다. A study for the assessment of dose given by outdoor radon to respiratory system has been carried out by making use of radon-cups containing CR-39 plastic track detectors. Detection efficiencies were determined by irradiation of the radon-cups in a standard radon chamber of known concentration. Thus determined detection factors of CR-39 plastic track detector in bare, open cup and filtered cup geometry are found to be 0.273, 0.0813 and 0.0371 trmm-2/(37 Bqm-3 d), respectively, which are chemically etched in 30% NaOH solution of 70℃ for 220 minutes. The outdoor radon concentrations measured at Taejeon (Chungnam National University) from May 1988 to March 1989 are in the range of 27.4 - 135.8 Bq/m3 (0.74 - 3.67pCi/l) by open cup and 16.7 - 143.9 Bq/m3 (0.45 - 3.89pCi/l) by filtered cup, which yield overall annual average value of outdoor radon concentration of 70.8 Bq/m3 (1.91 pCi/l). Corresponding effective dose equivalent rate to respiratory system of ICRP standard man is assessed to be 520nSv/h.
Band-to-Band 터널링에 의한 게이트 유기 드레인 누설전류의 이차원 수치해석
이상훈,하영철,송정근,홍창희 東亞大學校 附設 情報通信硏究所 1999 情報通信硏究所論文誌 Vol.7 No.1
In this paper, we investigated the characteristics of gate-induced drain leakage(GIDL) current due to the band-to-band tunneling in off-state n-MOSFET. Two-dimensional process and device simulation were performed by TMA-SUPREM4 and MEDICI. S/D(Single-Diffusion Drain) and LDD(Lightly Doped Drain) devices not applied electrical stress are simulated in order to compare drain engineering effect on GIDL characteristics. GIDL current due to direct interband tunneling in LDD structure was lower than that of S/D structure because of lower n-doping concentration. This result suggests that electric field in the gate-to-drain overlap rgion is a key factor controlling band-to-band tunneling generation rate.