Biochemical Adaptation of Pinus pumila on Low Temperature in Mt. Seorak, Korea

Kim Chan-Soo,Han Sim-Hee,Lee Wi-Young,Lee Jae-Cheon,Park Young-Ki,Oh Chang-Young 한국자원식물학회 2005 Plant Resources Vol.8 No.3

We tested the hypothesis that alpine plants have special physiological and biochemical mechanisms in addition to their structural adaptation in order to survive under extreme conditions. The photosynthetic organs of Pinus pumila were used to examine the seasonal changes in sugar concentration, antioxidative enzyme, and lipid peroxidation. The concentrations of sucrose, glucose, fructose and reducing sugar were the highest in the leaves in April. But sugar contents in buds and inner barks did not respond sensitively on temperature change. Meanwhile superoxide dismutase (SOD) activity responded sensitively on the change of temperature and SOD in all tissues maintained high activity in April. Meanwhile anthocyanin content increased rapidly in June but the increase of anthocyanin content was not enough to prevent their tissues from the damage by the exposure of high temperature or other stress. In conclusion, under low temperature condition, P. pumila increased the concentration of soluble sugars and SOD activity in their tissues in order to overcome extreme environmental condition. But in summer, these stress defense system against high temperature might be disturbed slightly. This results in the increase of malondialdehyde (MDA) contents in three tissues by lipid peroxidation.

안면골 골절에 관한 임상적 연구

심지영,오수진,장창덕,김영수 大韓顎顔面成形再建外科學會 1994 Maxillofacial Plastic Reconstructive Surgery Vol.16 No.1

This study was based on a series of 213 patients with facial bone fractures treated at college of Medicine, Dong-A university from Mar. 1990 to Jun. 1993. The results obtained were as follows : 1. The ratio of male to female was 8.3 : 1 and 3rd decade(34.3%) was the highest age group in incidence. 2. Monthly incidence was the highest in Jan. & Jun.(10.3%) 3. Onset time and daily incidence was the highest in pm. 9:00-12:00(21.4%), Sunday(18.8%). 4. Traffic acidents(38.5%) were the most frequent etiologic factor. 5. The most common site of fracture was zygoma & zygomatic arch(42.3%) and mandible (37.8%), maxilla (10.9%) and nasal bone(9.0%) were next in order of frequency. 6. In mandible fractures, the most frequent site was symphyseal area(43.0%) and IMF & open redction was major method of treatment. 7. In zygoma & zygomatic arch fractures, zygoma(62.0%) was the most common fracture site and open reduction was the most frequent treatment method. 8. In maxilla fractures, the major fracture type was Le Fort I type (72.4%) and treatment was done by IMF & open reduction primarily. 9. postoperative complications were mal-union, fibrous-union, infection, osteomyelitis etc.

RAW 264.7 세포에서 silica에 의한 유리 라디칼 생성에 미치는 protein kinase 억제제의 영향

심상수,조남영,윤미연,이지윤,김창종 중앙대학교 약학연구소 2002 약학 논총 Vol.16 No.-

To investigate involvement of protein kinase in reactive oxygen species (ROS) and peroxynitrite (PON) generation, we examined effect of protein kinase inhibitors on silica-induced ROS and PON generation in RAW 264.7 cells. Silica dose-dependently increased ROS and PON generation. Protein kinase C inhibitor, bisindolmaleimide and RO32-0432, slightly inhibited silica-induced ROS generation and significantly inhibited PON generation. Tyrosine kinase inhibitor, erbstatin and DHC, significantly inhibited silica-induced ROS generation but not PON generation. Calmodulin antagonist (W-7), calmodulin-dependent protein kinase Ⅱ inhibitor (KN-62) and mitogen-activated protein kinase inhibitor (PD98059) significantly inhibited silica-induced PON generation but not ROS generation. These results suggest that protein kinase C appears to be involved in both ROS and PON generation, tyrosine kinase in ROS generation, and calmodulin antagonist and mitogen-activated protein kinase in PON generation induced by silica in RAW 264.7 cells.

B16 melanoma 세포에서 nitric oxide가 멜라닌 생성에 미치는 영향

심상수,조남영,윤미연,이지윤,김창종 중앙대학교 약학연구소 2002 약학 논총 Vol.16 No.-

To investigate the effects of nitric oxide and cGMP on melanin production, we measured the melanin production in B16 melanoma cells stimulated by alpha-melanocyte stimulating hormone (MSH). NO donor, such as, sodium nitroprusside (SNP), SIN-1, SNAP and L-arginine increased melanin production in basal state. SNAP potentiated melanin production by MSH, but other NO donors did not. In the study the potentiating effect of sodium nitroprusside on cAMP-dependent melanin production, sodium nitroprusside did not increase melanin production induced by MSH, forskolin and 8-Br-cAMP. Non-selective cAMP/cGMP phosphodiesterase inhibitor (IBMX) and selective cGMP phosphodiesterase inhibitor (zaprinast) significantly increased MSH-induced melanin production, whereas soluble guanylyl cyclase inhibitor (ODQ) inhibited MSH-induced melanin production. These results suggest the possibility that MSH-stimulated melanin production may be mediated via not only cAMP-dependent pathway but also cGMP-dependent pathway in B16 melanoma cells.

Caerulein과 총담췌장관 결찰에 의한 급성췌장염 모델의 특징

심상수,김현준,이윤혜,박진형,이승준,김창종 중앙대학교 약학연구소 2000 약학 논총 Vol.14 No.-

To investigate the difference in acute pancreatitis experimentally induced by common bile-pancreatic duct (CBPD) ligation and caerulein iv infusion, we measured amylase activity, pancreatic edema and phospholipase A_2 activity in pancreatic tissue and serum. In acute pancreatitis induced by CBPD ligation and caerulein infusion, tissue edema and tissue phospholipase A_2 activity significantly increased as compared with normal group. Also experimental acute pancreatitis significantly increased the levels of serum protein, serum amylase activity and serum phospholipase A_2 activity. In the comparison of two experimental models, the symptom of acute pancreatitis induced by CBPD ligation was more severe than that induced by caerulein infusion. These results indicate that the increase of phospholipase A_2 activity in pancreatic tissue and serum may be one of the parameters for the diagnosis of acute pancreatitis.

유사량 산정공식에 따른 유사 및 하상변동 예측에 관한 연구

고수현,송인렬,심창석 한국환경과학회 2004 한국환경과학회지 Vol.13 No.3

The purpose of this study is to analyze the characteristics of riverbed variation due to the sediment protection weir located on the estuary of the main stream of Taehwa river using 1-D finite difference model, HEC-6 model, and the followings are the results of estimating sediment transport rate, amount of scour or deposition, and accumulated amount of deposit according to before and after of the sediment protection weir removal with various flow rates in the channel. Ackers-White transport function produced the greatest sediment transport rate while Meyer-Peter showed the smallest sediment transport rate at the most down stream area of the watershed through the sediment transport rate analyses for various flow rates according to the existence or nonexistence of the sediment protection weir. Toffaleti's and Colby transport function were closest to the average value, and the difference among the results of the sediment transport functions showed up to 8 ~9 times. Duboy's transport function produced the greatest riverbed variation while Toffaleti's showed the smallest variation through the riverbed variation analyses according to the existence or nonexistence of the sediment protection weir. Yang's was closest to the average value, and the difference among the results of the riverbed variation analyses ranged from 1.4 times to 11 times. It is thought that a sediment transport function must be selected very carefully with respect to the criteria of sediment yield estimation because the analysis results of the sediment transport rate and riverbed variation according to flow rates showed significant differences among the sediment transport functions, and the differences of sediment transport rate and riverbed variation according to the various sediment transport functions decreased as the flow rate increased.

효소결합면역분석법을 이용한 결핵성 능막염의 면역학적 진단법에 관한 연구

이춘택,심영수,김건열,한용철,조명제,차창룡 서울大學校 醫科大學 結核硏究所 1987 結核硏究所 硏究報告 Vol.1 No.1

후복막섬유증 5례의 임상적 고찰

정충식,김수현,안병수,심대성,박도영,김철성,장대수 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.1

Retroperitoneal fibrosis is a fibrotic process of the retroperitoneum that frequently produces ureteral obstruction. It can occur as an idiopathic disease or in association with various non-malignant and malignant condition. In the early phase of the retroperitoneal fibrosis ; symptoms originate from the disease process itself, while in the late state the clinical features represent the effect of obstructive uropathy and renal failure. Treatment consist of steroid therapy, urinary diversion and ureterolysis with jntraperitonealization or lateralization or wrapping with omentum. Herein we present 5 cases of retroperitoneal fibrosis.

Poster Session:PS 1203 ; Cardiology : Predictive Factors of Reversibility in Patients with Severe Tricuspid Regurgitation

( Jae Yeong Cho ),( Kye Hun Kim ),( Kyung Jin Lee ),( Yi Rang Yim ),( Sung Soo Kim ),( Hae Chang Jeong ),( Ki Hong Lee ),( Keun Ho Park ),( Doo Sun Sim ),( Hyun Ju Yoon ),( Nam Sik Yun ),( Young Joon 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1

Background:Since severe tricuspid regurgitation (TR) is an uncommon fi nding which develops in various conditions, the present study aimed to investigate etiology, clinical characteristics, and predictors of reversibility in patients with severe TR. Methods:A total of 232 patients (67. 4±14. 1 years, 80 males) who were diagnosed with severe TR by echocardiography were enrolled. Severe TR was defi ned as vena contracta width greater than 0. 7 cm and systolic fi ow reversal in hepatic veins according to the current guideline of American Society of Echocardiography (ASE). Improvement of TR to moderate or less degree on follow up echocardiography was considered as reversible TR in the present study. Primary end points were adverse events at long-term follow-up. Adverse events were defi ned as all-cause death and operation due to severe TR. Results: Reversible TR was observed in 35 out of 153 patients (23. 0%). Sixty-one patients showed clinical improvement, but the degree of TR was not changed. Forty-nine patients (32%) who did not show clinical improvement despite of medical therapy eventually underwent surgical correction of TR, and 7 patients died. In addition, 17 patients died without operation, so 24 deaths (16%) were observed in total. finally, total adverse events developed in 66 patients (41%) during 2 years of follow-up period. Logistic regression analysis revealed prosthetic mitral valve was the only independent predictors of future adverse events (OR 2. 47, 95%CI 1. 05-5. 77, p=0. 038). Independent predictors of improved TR turned out to be the use of RAAS blockade (HR 3. 02, 95%CI 1. 12-8. 17, p=0. 030) and the use of spironolactone (HR 3. 39, 95%CI 1. 05-10. 90, p=0. 041). Low ejection fraction (LVEF <50%) also had a trend toward the reversibility (HR 2. 82, 95%CI 0. 94-8. 40). Conclusions:Considering results above, reversible severe TR mainly associated with left heart failure and medical treatment should be given before considering surgery for severe TR.

필치가 혈중 알코올농도와 간세포내 알코올대사 효소 활성에 미치는 영향

이오미(O Mi Lee),예충민(Choong Min Ye),최병철(Byung Chul Choi),이지윤(Ji Yun Lee),강혜정(Hye Jeong Kang),최윤경(Yun Kyung Choi),김창종(Chang Jong Kim),심상수(Sang Soo Sim) 대한약학회 2005 약학회지 Vol.49 No.4

To investigate the effect of Feelch on alcohol metabolism, we measured both blood alcohol concentration in human and hepatic alcohol metabolizing enzyme activity in rats. The blood alcohol concentration in Feelch-ingested group was significantly lower than that in water-ingested group at 0, 40, and 80 minute after alcohol intake. The blood alcohol concentration between male and female taken 300 ㎖ of 21% alcohol showed the significant differences; the peak value of blood alcohol concentration in male and female were 0.0837±0.014% and 0.108±0.018%, respectively, In alcohol-fed rats, aldehyde dehydrogenase (ALDH) activity was significantly increased, whereas alcohol dehydrogenase (ADH) activity was not changed. In both Feelch-fed group and Feelch plus alcohol-fed group, ADH and ALDH activity were significantly increased as compared with each control group. Feelch decreased phospholipase A₂ activity and lipid peroxidation in hepatic tissue and activities of serum aminotransferases as compared with control. These results suggest that Feelch may have a hepato-protective effects and this is likely due to lower blood alcohol concentration via the increment of hepatic ADH and ALDH activity.