RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

덕유산 국립공원 등산로의 환경훼손에 대한 이용영향

서병수(Byung Soo Seo),김세천(Sei Cheon Kim),박종민(Chong Min Park),이창헌(Chang Heon Lee),이규완(Kyu Wan Lee) 한국산림과학회 1994 한국산림과학회지 Vol.83 No.3

The object of this study was to examine the user`s impacts on the environmental deteriorations of trail at Ticket Office - Paekryunsa (Temple) Hyangcho^kpong - Dongyupryung - Chilyun Fall area in To^kyusan National Park. Four trails were sampled in the study area according to the amount of users. Then the user`s impacts on trail were measured at each trail. The Ticket Office-Paekryunsa trail was the mo;;t used district and followed at Paekryunsa-Hyangcho^kpong trail, Hyangcho^kpong-Dongyupryung trail in descending order. Dongyupryung-Chilyun Fall trail is not used by people because of rest rotation system. The entire width of trail was greater at the more heavily used trail. Maximum depth, cross-sectional area loss, and surface texture and roughness of trail were the highest at Paekryunsa-Hyangcho^kpong trail. Soil hardness, soil acidity, soil moisture content, organic matter content, and exchange canon were influenced by trampling. Soil hardness, soil acidity and exchange canon increased in tramples soil, but content of soil moisture and organic matter decreased therein. Environmental deteriorations of trail were significantly influenced by the amount of users and the slope of trail. Bared lands about 2.000㎡ were appeared by trampling and camping around Hyangcho^kpong. Effects of carrying of rest rotation system for National Park were partly recognized at Dongyupryung-Chilyun Fall trail.

위발성 위장관 악성림프종 환자에서 항암치료의 효과

김찬규,신영록,김현정,배상병,이남수,이규택,박성규,원종호,홍대식,박희숙 순천향대학교 2006 Journal of Soonchunhyang Medical Science Vol.12 No.1

Purpose: The gastrointestinal (GI) tract is the most common site of extranodal non-Hodgkin's lymphoma (NHL), which is increasing in incidence, but there is no established optimal treatment modality. Thus, this study was investigated the clinicohistologic feature, the therapeutic modalities, and the prognosis for GI-NHL, as well as the factors affecting it. Methods: We retrospectively analyzed 45 patients who had been diagnosed as having GI-NHL and had been followed up from July 1994 to February 2005 at Soonchunhyang University Hospital. The patients were divided into groups according to the site of origin and to various other features, and the survivals of the various groups were compared. The modified Ann Arbor system and WHO classification were adopted for staging and histopathologic classification, respectively. Results: GI-NHL of the stomach, small bowel, ileocecal region, and colon occurred in 28 patients (62.2%), 5 patients (11.1%), 3 patients (6.7%), and 8 patients (17.8%), respectively, In one patient, the entire gastrointestinal tract was diffusely involved. The median age of patients was b5 years (25~78 years), and male-to-female ratio was 1:1.1. Fourteen patients were in stage Ⅰ, 24 in stage Ⅱ, 4 in stage Ⅲ, and 3 in stage Ⅳ. Surgical resection was performed in 19 patients, and combination chemotherapy was performed in 43 patients. Surgical resection only was performed in 4 patients, Chemotherapy only was performed in 26 patients. The expected overall 5 year survival of 45 patients was 39.6%, and there was a significant survival difference between the stages, but between sites of origin (p=0.842). The most important factors influencing the survival was the stage and other factors were not significant. Conclusion: The stomach was the most common site of GI-NHL. Most GI-NHL were localized Stage was the most important prognostic factor. However, Prospective randomized studies are needed to approve the therapeutic modality.

양측 쇄골하동맥 및 양측 신동맥 협착을 동반한 Takayasu 동맥염 1예

임선호,임수빈,오석규,이재홍,정진원,박양규,박옥규,소병준,노병석 대한순환기학회 1997 Korean Circulation Journal Vol.27 No.8

재발 또는 불응성 비호즈킨 림프종 환자에서 CDME 구제항암화학요법 후 고용량 항암화학요법 및 자가말초혈액 조혈모세포이식의 효과

김세형,한강원,배상병,김찬규,이남수,이규택,박성규,원종호,홍대식,박희숙 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Background and objectives : The long-term survival in patients with non-Hodgkin's lymphoma (NHL) after conventional dose chemotherapy is about 35% and the rest of the patients tend to have relapse. So, in relapsed or refractory NHL, we compared the outcome of patients undergoing high-dose chemotherapy with autologous peripheral blood stem cell transplantation(APBSCT) with only salvage chemotherapy of cisplatin, dexamethasone, mitoxantrone, and etoposide(CDME). Materials and methods : From June 1993 to December 1999, 25 patients with relapsed or resistant NHL were treated with CDME regimen as salvage chemotherapy. Twelve patients were received four cycles of CDME chemotherapy, and 13 patients were received high-dose chemotherapy with APBSCT following two cycles of CDME chemotherapy. Results : The median follow-up duration was 12.8 months(range:4-68). The overall response rate was 41.7% (complete response rate 25%, partial response rate 16.7%) in 12 patients with CDME only. Thirteen patients who were treated with high-dose chemotherapy with APBSCT achieved 61.5% complete response rate and 15.4% partial response rate, with an overall response rate of 76.9%. The estimated 3-year progression-free survival rate was significantly higher among patients who received high-dose therapy than patients who received CDME only(41.5% vs 20.0%, p<0.05). And, 3-year overall survival rate was significantly higher among patients who received high-dose therapy(51.3% vs 25.0%, p <0.05). Conclusions : In relapsed or refractory NHL, CDME chemotherapy is an effective salvage chemotherapy and allow peripheral blood stem cell collection. Also, high-dose chemotherapy with APBSCT following CDME is superior to CDME salvage chemotherapy only.

흉선종 제거후에 발생한 재생 불량성 빈혈 : 증례보고

전원선,이상철,김현정,배상병,김찬규,이남수,박노진,이규택,박성규,홍대식,박희숙,원종호 순천향의학연구소 2007 Journal of Soonchunhyang Medical Science Vol.13 No.2

Thymoma is associated with myasthenia gravis, Pure red cell aplasia, and autoimmune diseases such as autoimmune hemolytic anemia, aplastic anemia, and hypogammaglobulinemia. It's association with aplastic anemia is rare and aplastic anemia appearing after surgical removal of thymoma is especially rare. The authors hereby report a case of aplastic anemia occuring in a patient who was diagnosed with thymoma and myasthenia gravis and had the tumor surgically removed. The patient was treated with anti-thymocyte globulin, cyclosporin, and prednisolone, and showed partial remission with hematologic improvements after 12 months.

운동선수의 임장불안에 대한 연구

홍관이,이광재,김두경,부기원,노성규,한상준,박기동,오수일,문병용,이철규,박장평,엄기진,박남환 江原大學校附設 體育科學硏究所 1984 江原大學校附設體育科學硏究所論文集 Vol.- No.9

This study is designed to grasp the elements and situations of stage fright in order to propose a part of ways to cope with it. We make 511 top-class high school players, male and female, in the central districts (Seoul, Inchon, Kyungki, Chungbuk, and Kangweon Province) on object of questionnaire, and get the following results. Through players generally have all-round characters they show weakness in the superiority and emotional stability while showing strength in the sonformity. This fact comes to be related to the elements which cause stage fright. Physiological changes also have many effects on the symptom of stage fright. Especially, stage fright increases when players show sensitive reaction to the self-consciousness of final consequence which comes from the tension or uneasiness of sympathetic nerve, Thus, it turns out that players make use of reducing psychological burden by physical exercise or adjusting the level of demanded result in order to release the stage fright.

근신경학(neuro-myology)에서의 근질환에 대한 연구

박병규 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.5

The neuromuscular facility of muscle and nerve plays an important role in keeping the muscular trophic state in normal condition, and its intervention is essential in connection with neuromuscular facility. Tendon spindle is mainly located at myotendon junction, which is a small size of particularized muscular fiber to control the contraction and expansion of muscle. All the receptors exist in fine motor function-related muscles at higher density, controled by many different kinds of muscles and nerves. With respect to the muscular transmission available to measure the stabilized level of neuromuscular stimulation, the cramps are graphically shown by a high frequency of radiation. Based on fact that there is no a certain type of continued pattern resulting in a disease, it has no any pathological importance. Because a serious degree of cramps are easily caused even by ordinary movement and then continued in existence, nevertheless, they deadly stimulate the functions of major and other muscles. In fact, all kinds of cramps have only the sensation of cramp without any real muscular contraction, being considered to be a dysesthetic phenomenon. As a result, the muscular cramps are the unstabilized pattern of the nervous system, in terms of muscle and nerve. These individual conditions cause spasm and cramp which obstruct effective and voluntary motion with various pains. Also, they have their own clinical and myokinetic characteristics which are mostly well responsive to cure. In order to prevent the muscular cramps from being caused during sporting activities, it is required to carry out more studies on the mechanism concerning their causes. Other studies on muscular diseases showed that all kinds of mechanism relating to muscle contraction are created by the smooth balance of muscles and nerves. Accordingly, the clinical diagnosis of these conditions has been considered to be important.

Nutcracker 증후군의 진단에 있어서 도플러 초음파검사의 유용성 : Diagnostic Usefulness of Doppler US

박병관,김승협,조정연,성창규,박선원,한만청 대한초음파의학회 1998 ULTRASONOGRAPHY Vol.17 No.2

생리적 및 염증성 유치 치근 흡수면의 미세구조

박윤희,손흥규,최병재 대한소아치과학회 2000 대한소아치과학회지 Vol.27 No.4

유치 치근 흡수가 일어나는 경우는 임상에서 많이 접할 수 있다. 유치 치근 흡수는 나이에 따른 생리적 흡수와 치아우식증 및 외상 등으로 비정상적으로 흡수되는 염증성 흡수로 나눌 수 있다. 염증성 흡수는 그 시기와 양상이 생리적인 치근 흡수와는 다르며, 흡수기전이나 흡수면의 미세구조에 있어서도 많은 차이가 있을 것으로 사료된다. 유치 치근 흡수면의 미세구조에 대한 연구는 많이 있었으나, 생리적 흡수면과 염증성 흡수면의 미세구조를 비교한 연구는 드물다. 본 연구는 생리적 흡수면과 염증성 흡수면의 형태 및 인접한 세포를 연구하기 위하여 생리적 및 염증성 흡수로 인해 발거된 유치 치근의 흡수 표면을 주사전자현미경상으로, 조직학적 형태를 광학현미경상으로 관찰하여 다음과 같은 차이점을 알 수 있었다. 1.주사전자현미경상에서 생리적 유치 치근 흡수 표면은 타원형, 원형 및 다각형 모양의 흡수소와가 관찰되었고, 염증성 흡수 표면은 크기가 작으며 형태가 다양하고 매우 불규칙한 무정형 형태의 흡수소와가 관찰되었다. 2.광학현미경상에서 생리적 유치 치근 흡수면은 흡수소와로 구성된 규칙적인 큰 흡수와가 관찰되었고, 염증성 흡수면은 불규칙한 흡수소와가 관찰되었다. 3.광학현미경상에서 생리적 유치 치근 흡수면에는 다핵거대세포가 흡수소와에 직접 접하고 있었으며, 염증성 흡수면은 간엽세포 및 염증세포가 많이 관찰되었다. 4.광학현미경상에서 염증성 치근 흡수면에서는 상아질 흡수를 보상하는 부분적인 백악질 형성이 관찰되었다. Deciduous teeth can be extracted for two reasons, one due to the physiologic resorption and the other by the inflammation at the apex after traumatic injury. Physiologic resorption may be different from pathologic resorption in timing and mechanism. Therefore we resumed the different features of physiologic and pathologic resorption root surfaces. Many previous studies showed micromorphology of resorbed surface of roots of deciduous teeth. But, few studies compared physiological and pathological root resorption surfaces. In this study, we carefully observed microscopic morphologies of those two different root surfaces by scanning electron microscope and histologic features by light microscope. The resultant differences between physiologic and pathologic resorption surfaces of deciduous teeth were as follows: 1. The morphology of pathologic resorption lacunae due to inflammation varied in size and shape with irregular boundaries compared with the physiologic areas from scanning electron microscope observations. 2. From light microscope observations, several large resorption fossae containing numerous resorption lacunae were found, whereas the resorption lacunae were irregular in shape with pathologic resorption surface. 3. Numerous multinucleated giant cells were closely attached to the physiologic resorption lacunae, whereas several kinds of mesenchymal cells with numerous inflammatory cells were found in the areas adjacent to the pathologic resorption surface. 4. Light microscope findings showed that compensating cementum formation took place along some of the areas of inflammatory dentinal resorption. In conclusion, several morphological differences were between physiologic and pathologic root resorption surfaces of human deciduous teeth. The future studies should include cytochemisty to clarify the cellular roles in resorption process observations of pulpal surfaces of coronal and radicular dentin to find the changes that occur in each phase of human deciduous tooth resorption.