RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

덕유산 국립공원 등산로의 환경훼손에 대한 이용영향

서병수(Byung Soo Seo),김세천(Sei Cheon Kim),박종민(Chong Min Park),이창헌(Chang Heon Lee),이규완(Kyu Wan Lee) 한국산림과학회 1994 한국산림과학회지 Vol.83 No.3

The object of this study was to examine the user`s impacts on the environmental deteriorations of trail at Ticket Office - Paekryunsa (Temple) Hyangcho^kpong - Dongyupryung - Chilyun Fall area in To^kyusan National Park. Four trails were sampled in the study area according to the amount of users. Then the user`s impacts on trail were measured at each trail. The Ticket Office-Paekryunsa trail was the mo;;t used district and followed at Paekryunsa-Hyangcho^kpong trail, Hyangcho^kpong-Dongyupryung trail in descending order. Dongyupryung-Chilyun Fall trail is not used by people because of rest rotation system. The entire width of trail was greater at the more heavily used trail. Maximum depth, cross-sectional area loss, and surface texture and roughness of trail were the highest at Paekryunsa-Hyangcho^kpong trail. Soil hardness, soil acidity, soil moisture content, organic matter content, and exchange canon were influenced by trampling. Soil hardness, soil acidity and exchange canon increased in tramples soil, but content of soil moisture and organic matter decreased therein. Environmental deteriorations of trail were significantly influenced by the amount of users and the slope of trail. Bared lands about 2.000㎡ were appeared by trampling and camping around Hyangcho^kpong. Effects of carrying of rest rotation system for National Park were partly recognized at Dongyupryung-Chilyun Fall trail.

위발성 위장관 악성림프종 환자에서 항암치료의 효과

김찬규,신영록,김현정,배상병,이남수,이규택,박성규,원종호,홍대식,박희숙 순천향대학교 2006 Journal of Soonchunhyang Medical Science Vol.12 No.1

Purpose: The gastrointestinal (GI) tract is the most common site of extranodal non-Hodgkin's lymphoma (NHL), which is increasing in incidence, but there is no established optimal treatment modality. Thus, this study was investigated the clinicohistologic feature, the therapeutic modalities, and the prognosis for GI-NHL, as well as the factors affecting it. Methods: We retrospectively analyzed 45 patients who had been diagnosed as having GI-NHL and had been followed up from July 1994 to February 2005 at Soonchunhyang University Hospital. The patients were divided into groups according to the site of origin and to various other features, and the survivals of the various groups were compared. The modified Ann Arbor system and WHO classification were adopted for staging and histopathologic classification, respectively. Results: GI-NHL of the stomach, small bowel, ileocecal region, and colon occurred in 28 patients (62.2%), 5 patients (11.1%), 3 patients (6.7%), and 8 patients (17.8%), respectively, In one patient, the entire gastrointestinal tract was diffusely involved. The median age of patients was b5 years (25~78 years), and male-to-female ratio was 1:1.1. Fourteen patients were in stage Ⅰ, 24 in stage Ⅱ, 4 in stage Ⅲ, and 3 in stage Ⅳ. Surgical resection was performed in 19 patients, and combination chemotherapy was performed in 43 patients. Surgical resection only was performed in 4 patients, Chemotherapy only was performed in 26 patients. The expected overall 5 year survival of 45 patients was 39.6%, and there was a significant survival difference between the stages, but between sites of origin (p=0.842). The most important factors influencing the survival was the stage and other factors were not significant. Conclusion: The stomach was the most common site of GI-NHL. Most GI-NHL were localized Stage was the most important prognostic factor. However, Prospective randomized studies are needed to approve the therapeutic modality.

재발 또는 불응성 비호즈킨 림프종 환자에서 CDME 구제항암화학요법 후 고용량 항암화학요법 및 자가말초혈액 조혈모세포이식의 효과

김세형,한강원,배상병,김찬규,이남수,이규택,박성규,원종호,홍대식,박희숙 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Background and objectives : The long-term survival in patients with non-Hodgkin's lymphoma (NHL) after conventional dose chemotherapy is about 35% and the rest of the patients tend to have relapse. So, in relapsed or refractory NHL, we compared the outcome of patients undergoing high-dose chemotherapy with autologous peripheral blood stem cell transplantation(APBSCT) with only salvage chemotherapy of cisplatin, dexamethasone, mitoxantrone, and etoposide(CDME). Materials and methods : From June 1993 to December 1999, 25 patients with relapsed or resistant NHL were treated with CDME regimen as salvage chemotherapy. Twelve patients were received four cycles of CDME chemotherapy, and 13 patients were received high-dose chemotherapy with APBSCT following two cycles of CDME chemotherapy. Results : The median follow-up duration was 12.8 months(range:4-68). The overall response rate was 41.7% (complete response rate 25%, partial response rate 16.7%) in 12 patients with CDME only. Thirteen patients who were treated with high-dose chemotherapy with APBSCT achieved 61.5% complete response rate and 15.4% partial response rate, with an overall response rate of 76.9%. The estimated 3-year progression-free survival rate was significantly higher among patients who received high-dose therapy than patients who received CDME only(41.5% vs 20.0%, p<0.05). And, 3-year overall survival rate was significantly higher among patients who received high-dose therapy(51.3% vs 25.0%, p <0.05). Conclusions : In relapsed or refractory NHL, CDME chemotherapy is an effective salvage chemotherapy and allow peripheral blood stem cell collection. Also, high-dose chemotherapy with APBSCT following CDME is superior to CDME salvage chemotherapy only.

흉선종 제거후에 발생한 재생 불량성 빈혈 : 증례보고

전원선,이상철,김현정,배상병,김찬규,이남수,박노진,이규택,박성규,홍대식,박희숙,원종호 순천향의학연구소 2007 Journal of Soonchunhyang Medical Science Vol.13 No.2

Thymoma is associated with myasthenia gravis, Pure red cell aplasia, and autoimmune diseases such as autoimmune hemolytic anemia, aplastic anemia, and hypogammaglobulinemia. It's association with aplastic anemia is rare and aplastic anemia appearing after surgical removal of thymoma is especially rare. The authors hereby report a case of aplastic anemia occuring in a patient who was diagnosed with thymoma and myasthenia gravis and had the tumor surgically removed. The patient was treated with anti-thymocyte globulin, cyclosporin, and prednisolone, and showed partial remission with hematologic improvements after 12 months.

양측 쇄골하동맥 및 양측 신동맥 협착을 동반한 Takayasu 동맥염 1예

임선호,임수빈,오석규,이재홍,정진원,박양규,박옥규,소병준,노병석 대한순환기학회 1997 Korean Circulation Journal Vol.27 No.8

운동선수의 임장불안에 대한 연구

홍관이,이광재,김두경,부기원,노성규,한상준,박기동,오수일,문병용,이철규,박장평,엄기진,박남환 江原大學校附設 體育科學硏究所 1984 江原大學校附設體育科學硏究所論文集 Vol.- No.9

This study is designed to grasp the elements and situations of stage fright in order to propose a part of ways to cope with it. We make 511 top-class high school players, male and female, in the central districts (Seoul, Inchon, Kyungki, Chungbuk, and Kangweon Province) on object of questionnaire, and get the following results. Through players generally have all-round characters they show weakness in the superiority and emotional stability while showing strength in the sonformity. This fact comes to be related to the elements which cause stage fright. Physiological changes also have many effects on the symptom of stage fright. Especially, stage fright increases when players show sensitive reaction to the self-consciousness of final consequence which comes from the tension or uneasiness of sympathetic nerve, Thus, it turns out that players make use of reducing psychological burden by physical exercise or adjusting the level of demanded result in order to release the stage fright.

E. coli 과량발현을 통한 B형 간염 바이러스 preS1 유도체의 안정한 동위원소 N의 치환

박병관,김세하,한규훈,김선영,홍요정,이시형,한동설,김순종,박진구 木浦大學校基礎科學硏究所 1997 基礎科學硏究誌 Vol.15 No.-

We have used molecular biology technique to label the side chains and backbone of HBV preS1(1-56) with stable isotope (??) during E. coli over-expression and purified the protein to near homogeneity. The expression level of ?? labelled preS1(1-56) seems to be ∼15% of the total protein. to characterize the secondary structure of the labelled protein, circular dichroism was used. In different buffer conditions including pH 3.3 and pH 7.4 the protein seems to be devoid of any stable secondary structure probably suggesting that the N-terminal portion (preS1(1-56)) of HBV preS1 might be unstructured before binding to its target cells.

정형의학적 측면에서의 근신경 검사법

박병규 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.5

It seems that a variety of muscular diseases result from the abnormal neuromuscular system, To understand the abnormal muscular mechanism, it is absolutely required to know in advance about the physiological and biochemical aspects relating to the contraction of muscular fibers, nerve action potential and neuromuscular transmission. It is not possible, of course, to understand all the biochemical and biophysical aspects concerning the creation and transmission of a nerve impulse. Many studies have been carried out since early studies done by Hodgkin (1951) and Hodgkin & Huxley (1951). Like other somatic cells, neuromuscular cells has the environment of the intracellular fluid which is definitely different from the extracellular or interstitial fluid. While its intracellular component includes potassium (K), magnesium (Mg) and phosphorous (P), sodium calcium (Ca) and chloride (Cl) are the major ectracellular component, The intracellular ion concentration inside nerve muscle is kept within a narrow range by an electric and chemical energy which maintains the cellular wall balanced electrically and chemically ("stable wall potential"). It seems that these energies make the smooth neuromuscular action be accomplished by the pump function (sodium pump) which selectively pumps a variety of ions into cellular walls, as well as sodium within cells and, therefore, muscular action can be created. Although some basic studies have been performed on the mechanism of neuromuscular interaction, further studies are required to examine the mechanism leading to diagnosis in disease of nerve and muscle, abnormal syndrome, and their preventative measures.

아크 용접작업에서의 방진마스크 포집효율에 관한 조사

박동기,안규동,이병국 순천향대학교 산업의학연구소 1997 순천향산업의학 Vol.3 No.1

The results of survey on filtering efficiency of tight fitting respirators and disposable dust respirators which are mostly used at arc-welding works are as follows; 1. The mean efficiency of two tight fitting respirators(made by "M" and "MA") marked with "Dust-mist" for 10, 30 and 60 minutes is 60.6% and that of three disposable dust respirators(one piece of "M" maker's and two pieces of "W" maker's) with same mark is 65.7% much less than 97.1% of "M" maker's one disposable dust respirator marked with "Dust-mist-fume". 2. The mean efficiency of four tight fitting respirators for 10 minutes is 40.8-88.35% and thant of eight disposable dust respirators mean 52.7-97.2%. 3. The efficiency of both types marked with "Dust-mist" for 10 minutes is lower than that for 60 minutes but higher than that for 30 minutes whereas that of respirator made with "Dust-mist-fume" is almost constant. One the other hand, the efficiency of disposable dust respirators marked with "Dust-mist" is 5.1% higher than that of tight fitting respirators with same mark. 4. They are investigated after years that it is the efficiency of dispoasble dust respirators marked with "Dust-Mist" 5.1% higher than of tight fitting respirators with same mark and that the efficiency of both types marked with "Dust-Mist" for 10 minutes is lower than that for 60 minutes but higher than thant for 30 minutes, respectively. According to the results, it should be recommended that are welding workers must wear the respirator for fume. Furthermore the testing methods for mineral dust (SiO_(2) : Concentration, Particle size, etc.) should not be applied to all types of dust masks, but testing standards in accordance with the size of fume and mist should be established, which the developed countries have.