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Lentinus edodes 에서 분비되는 Laccase 의 특성
정인범,윤홍덕,맹진수,강사욱,하영칠,정가진,최형태,김재헌 한국미생물학회 1992 미생물학회지 Vol.30 No.4
Lentinus edodes 에서 분비된 laccase 를 DEAE Sephadex A-50, Con A-Sepharose, Sephadex G-150 크로마토그래피를 통해서 순수분리하였다. 효소는 분자량이 87 KDa 정도인 하나의 소단위체로 되어 있고, 12.0% 의 당을 함유하고 있었다. 그리고 N-말단 아미노산 서열은 Pleurotus oxtreatus 와 Cloriolus hirsutus 의 laccase 와 유사하였다. 효소의 최적 pH 는 4.8 이고 최적 온도는 $40^{\circ}C$ 이었다. 그리고 본 효소는 pH 7-9 와 $30^{\circ}C$이하에서 비교적 안정하였다. Syringaldazine 에 대한 $K_{M}$ 은 $0.4\mu$ M 이었고 $k_{cat}$ 은$ 77 sec^{-1}$ 이었다. This layer chromatography 에 의한 본 효소의 반응 산물들의 분리양상이 Pleurotus ostreatus 의 laccase 의 경우와 유사하였다. Extracellular laccase excreted from Lentinus edodes ATCC 48085 was purified through a series of DEAF, Sephadex A-50. Con A-Sepharosc and Sephadex G-150 chromatography. Extracellular enzyme. which consists of a single polypeptide, has a n~olecular mass of 87.000 daltons and contains 12.0'%, carbohydrate. The N-terminal amino acid sequence (I5 residues) of the puritied enzyme was similar to that of laccases of PIeurotus ostreatus and Coriolus hirsutus. The enzyme showed optimal activity at near pH 4.8 and $40^{\circ}C$. The enzyme was stable at pH 7-9 and below $30^{\circ}C$. $K_{M}$ and $k_{cat}$ values for syringaldazine were estimated to be $0.4\mu\textrm{M}$ and 77 sec, respectively. The developed patterns of reaction products of thevenzyme on thin layer chromatography were similar to those of laccase of Pleurotus ostreatus.
정인범,Moon Jun Na,Ji Woong Son,Do Yeon Jo,권선중 대한중환자의학회 2016 Acute and Critical Care Vol.31 No.4
Background: Several antibiotics can be used to treat ventilator-associated pneumonia caused by carbapenem-resistant A. baumannii (CRAB-VAP) including high-dose sulbactam. However, the effectiveness of high-dose sulbactam therapy is not well known. We report our experience with high-dose sulbactam for treatment of CRAB-VAP. Methods: Medical records of patients with CRAB-VAP who were given high-dose sulbactam between May 2013 and June 2015 were reviewed. Results: Fifty-eight patients with CRAB-VAP were treated with high-dose sulbactam. The mean age was 72.0 ± 15.2 years, and the acute physiology and chronic health evaluation II (APACHE II) score was 15.1 ± 5.10 at the time of CRAB-VAP diagnosis. Early clinical improvement was observed in 65.5% of patients, and 30-day mortality was 29.3%. Early clinical failure (odds ratio [OR]: 8.720, confidence interval [CI]: 1.346-56.484; p = 0.023) and APACHE II score ≥ 14 at CRAB-VAP diagnosis (OR: 10.934, CI: 1.047-114.148; p = 0.046) were associated with 30-day mortality. Conclusions: High-dose sulbactam therapy may be effective for the treatment of CRAB-VAP. However, early clinical failure was observed in 35% of patients and was associated with poor outcome.
정인범,손지웅,나문준,권선중,김지혜,오태정 대한결핵 및 호흡기학회 2016 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.121 No.-
Bronchoscopy is the most important procedure to diagnose lung cancer. However, the sensitivity of bronchoscopy is low(34~88%). Moreover, cytologic evaluation of bronchial washing, which is a routine adjunctive test, does not improve the performance of bronchoscopy due to its low sensitivity(28~32%). Non-diagnostic bronchoscopy warrants further invasive procedure or radiologic surveillance, both of which carry significant risk of complications or delayed diagnosis. To enhance the diagnostic performance of bronchoscopy, we introduced the PCDHGA12 methylation biomarker of bronchial washing as a novel adjunctive diagnostic test. A total of 98 patients suspicious for lung cancer (final diagnosis concluded 60 patients were lung cancer and 38 were benign) that underwent bronchoscopy with bronchial washing were enrolled. Performance of the PCDHGA12 methylation biomarker was tested. The sensitivity of the PCDHGA12 methylation biomarker was 75.0%, and the negative predictive value(NPV) was 66.7%, while the sensitivity and NPV of cytology was 45.0% and 51.4%, respectively. Moreover, when the group patients not diagnosed as lung cancer by bronchoscopy were considered separately, the biomarker showed a sensitivity of 61.9% and a NPV of 78.9%. The PCDHGA12 methylation biomarker is a useful adjunctive test for bronchoscopy for determining probability of lung cancer before further invasive procedure.