인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

녹색건축 전문가 자격제도 개발 연구

이상민,이승민,Lee, Sang Min,Lee, Seung Min 한국교육녹색환경연구원 2014 교육·녹색환경연구 Vol.13 No.1

In an effort to resolve environmental pollution problems, developed countries enforce green building certification systems. The same is true in Korea. However compared professional accreditation system to certification systems did not systematically in Korea. Therefore, in accordance with development of green building related systems and technology the requirement for foster green building professionals is urgent. Accordingly this study is purposed to analyze and propose that green building professional accreditation system development and operation of the direction. Through data analysis domestic and international green building professional accreditation system for draw a development plan. And then conduct an expert Delphi survey reflected in the survey results to development plan.

헤어리베치(Vice villosa Roth) 품종별 국내 월동성, Biomass 및 무기태 질소 함량비교

이상민,이연,이용환,성좌경,윤홍배,최현석,Lee, Sang-Min,Lee, Youn,Lee, Yong-Hwan,Sung, Jwa-Kyung,Yun, Hong-Bae,Choi, Hyun-Sug 한국유기농업학회 2013 韓國有機農業學會誌 Vol.21 No.1

헤어리베치 8종의 품종별 국내 적응성 검증을 위해 월동률과 biomass, 그리고 토양 중 무기태질소를 조사하였다. 겨울철 월동 후 생존율은 Hungvillosa와 Ostsaat 품종에서 좋았고, Minnie, Oregon common과 TTF1에서 낮았다. 생존율은 biomass에도 영향을 주어서 Hungvillosa와 Ostsaat 품종에서 가장 높았다. 헤어리베치 품종 간 무기성분 농도는 별다른 차이가 관찰되지 않았다. 토양 중 무기태질소 농도는 질소환원능이 좋았던, Hungvillosa와 Ostsaat가 예초 후 10여일 후에 크게 증가시켰다. This study was conducted to evaluate overwintering of hairy vetch (Vicia villosa Roth), biomass, and inorganic N concentration in soil to verify the adaptability of eight cultivars of hairy vetch into country. Winter survival rate was higher for the hairy vetch cultivars, such as Hungvillosa or Ostsaat, than those of Minnie, Oregon common, and TTF1, which affected amount of biomass of each hairy vetch, with higher biomass observed in Hungvillosa or Ostsaat cultivar. There were no significant difference for the mineral nutrients of each hairy vetch cultivar. Inorganic N concentration in soil was increased at 10 days after mowing by application of Hungvillosa and Ostsaat cultivars that had highly produced N content from the raw materials.

대장직장 종양에 대한 내시경 점막하 절제술의 연대순 임상 결과와 학습 곡선

이상민 ( Sang Min Lee ),김은수 ( Eun Soo Kim ),박경식 ( Kyung Sik Park ),조광범 ( Kwang Bum Cho ),김동춘 ( Dong Choon Kim ),강유진 ( Yu Jin Kang ),이유진 ( Yoo Jin Lee ),이정민 ( Jung Min Lee ),최은성 ( Eun Sung Choi ),최재혁 ( Ja 대한소화기학회 2014 대한소화기학회지 Vol.64 No.4

Background/Aims: Endoscopic submucosal dissection (ESD) is an effective procedure for en-bloc curative resection of the colorectal tumor. As it requires high technical skills and experience in therapeutic endoscopy, it is important to understand learning curve of ESD technique. The aim of this study was to retrospectively describe the clinical results of ESD and to evaluate learning curve for the ESD of colorectal tumors. Methods: A total of 90 patients with 90 colorectal neoplasms, who had undergone ESD at a tertiary referral hospital from July 2009 to December 2012, were enrolled. The ESD was performed by a single endoscopist. All ESD cases were divided into three periods: first, cases 1-30; second, cases 31-60; and third, cases 61-90. Results: The en-bloc resection rates in third period (100%) was significantly higher than that of the first (93.3%) and second period (80%) (p=0.025). The perforation rate in third period (0%) also significantly decreased compared with that of the first (13.3%) and second period (20%) (p=0.032). To calibrate the difference of tumor size among periods, proficiency was calculated, as the procedure time per specimen area (min/cm2). The proficiency in third period (4.3) was significantly shorter than that of the first (16.8) and second period (10.2) (p=0.004). Conclusions: The learning curve of colorectal ESD in our study shows that at least 60 cases of ESD have to be conducted to acquire sufficient skill of degree without perforation. (Korean J Gastroenterol 2014;64:198-205)

대식세포주에서 인슐린이 $I{\kappa}B/NF-{\kappa}B$ 경로 활성화에 미치는 영향

이상민,장연실,이춘택,김영환,한성구,심영수,유철규,Lee, Sang-Min,Jang, Yeon-Sil,Lee, Choon-Taek,Kim, Young-Whan,Han, Sung-Koo,Shim, Young-Soo,Yoo, Chul-Gyu 대한결핵및호흡기학회 2010 Tuberculosis and Respiratory Diseases Vol.68 No.3

Background: Sepsis still has a high mortality rate despite adequate supportive care. Newer therapeutic modalities have been developed but they have generally ended in failure. Recently, insulin was reported to have an anti-inflammatory effect by inhibiting the $I{\kappa}B/NF-{\kappa}B$ pathway, and may have therapeutic potential in sepsis. However, the precise mechanism of the anti-inflammatory effect of insulin is unclear. This study examined the role of insulin in activating $I{\kappa}B/NF-{\kappa}B$ in macrophage. Methods: Raw 264.7 cells, a murine macrophage cell line, were used in this experiment. Western blotting using $I{\kappa}B$ Ab and phosphor-specific $I{\kappa}B$ Ab was performed to evaluate the degradation and phosphorylation of $I{\kappa}B$ cells. For the $I{\kappa}B$ Kinase (IKK) activity, an immune complex kinase assay was performed. The level of interleukin-6 (IL-6) was measured by ELISA to determine the level of proinflammatory cytokine. Results: $I{\kappa}B{\alpha}$ degradation began 30 min after lipopolysaccharide (LPS) treatment. However, an insulin pretreatment suppressed the $I{\kappa}B{\alpha}$ degradation caused by the LPS treatment. The phosphorylation of $I{\kappa}B{\alpha}$ and IKK activity was also inhibited by the insulin pretreatment. Finally, the insulin pretreatment showed a tendency to suppress the induction of IL-6 by LPS. Conclusion: Insulin might have an anti-inflammatory effect though partial inhibition of the $I{\kappa}B/NF{\kappa}B$ pathway in macrophage cell lines.

녹비작물을 이용한 윤작이 잡초 제어에 미치는 영향

이상민,이병모,이연,이용환,성좌경,윤홍배,최현석,Lee, Sang-Min,Lee, Byung-Mo,Lee, Youn,Lee, Yong-Hwan,Sung, Jwa-Kyung,Yun, Hong-Bae,Choi, Hyun-Sug 한국유기농업학회 2012 韓國有機農業學會誌 Vol.20 No.2

본 시험은 유기농업 파 및 고추재배를 위하여 호밀과 헤어리베치를 겨울에 윤작하여 잡초 발생량 억제에 미치는 효과를 검토하기 위하여 수행되었다. 파 재배구에서 호밀을 토양에 환원하지 않은 처리구에 비해 호밀을 환원하였을 경우 잡초발생량을 52% 경감시켰다. 고추 재배구에서는 그러한 효과가 뚜렷하게 관찰되지 않았다. 파 재배시 어저귀, 참방동사니, 바랭이와 같은 초종은 호밀뿌리 분비물에 의해 발아가 억제되는 경향이었으나 헤어리베치는 그러한 발생억제 효과가 크지 않았다. 파 재배시 호밀 환원은 잡초발생량을 경감시켰고 잡초의 종 다양성 지수를 증가시켰다. This study was conducted to evaluate the effects of crop rotation on the weed occurrence in green onion and red pepper cultivation. Rye and hairy vetch were annually seeded for winter season. The reduction of weed germination was pronounced by about 50% in green onion field, but was not significant in red pepper field through rye cropping. Among weed species, the germination of Abutilon theophrasti, Cyperus iria and Digitaria ciliaris was distinctly inhibited by exudate of rye root, but not by that of hairy vetch in vitro experiment. The return of rye biomass into onion field decreased amount of weed biomass but increased index of weed diversity.

선진국의 바이오에너지 정책과 영향

이상민,Lee, Sang-Min 한국사료협회 2007 飼料 Vol.6 No.4

조피볼락 Sebastes schlegeil의 n-3계 고도불포화지방산 요구량

이상민,이종윤,강용진,윤호동,허성범,LEE Sang-Min,LEE Jong Yun,KANG Yong Jin,YOON Ho-Dong,HUR Sung Bum 한국수산과학회 1993 한국수산과학회지 Vol.26 No.5

1. 조피볼락의 적정성장에 필요한 지방산을 조사하기 위해 사료의 지방산종류 (fat-free, 12:0, 18:1n-9, 18:2n-6, 18:3n-3 및 n-3HUFA)가 다른 6종의 실험사료로 44g 전후의 조피볼락을 9주간 사육한 결과, fat-free, 12:0, 18:1n-9, 18:2n-6 및 18:3n-3 첨가구보다 n-3HUFA 첨가구가 성장 및 사료효율에서 좋은 결과를 보였다. 2. 조피볼락의 n-3HUFA 요구량을 구명하기 위해, 사료지질 $8\%$ 범위에서 기본지질을 우지로 하여 정제 지방산 (n-3HUFA $85\%$)을 첨가, n-3HUFA 함량이 $0{\sim}4.0\%$가 되도록 조정한 10종의 실험사료로 평균 체중 5.9g의 조피볼락 치어를 6주간 사육한 후, 성장 및 체성분의 변화를 조사하였다. 증중률 ($117{\sim}297\%$) 및 사료효율($67{\sim}96\%$)모두 n-3HUFA 무첨가 사료에서 가장 낮은 값을 나타내었으며, 사료 n-3HUFA 함량의 증가와 더불어 그 효과가 현저히 개선되다가 $0.9\%$ 이후에는 더이상 개선되지 않았다. 따라서, 조피볼락의 n-3HUFA 요구량은 $0.9\%$로 추정되었다. 사료의 n-3HUFA 함량이 증가함에 따라 간지질 함량과 간중량 비는 감소하는 경향을 보였으며, 간 glycogen 함량은 증가하였다. 간 극성지질의 지방산조성은 사료의 지방산조성에 따라 변화하여, 사료의 n-3HUFA가 증가함에 따라 18:1은 감소하고, EPA와 DHA같은 n-3HUFA는 증가하였다. 그리고 간 극성지질의 18:1/n-3HUFA 비는 사료의 n-3HUFA 증가와 더불어 감소하였다. In order to investigate the n-3 highly unsaturated fatty acids (n-3HUFA) requirement of the Korean rockfish Sebastes schlegeli, two experiments were conducted in the flush-out aquarium system. 1. Effects of different dietary fatty acids on growth and feed efficiency Efficacy of different fatty acids on the Korean rockfish was investigated by feeding diets containing each of the different fatty acids, 12:0, 18:1n-9, 18:2n-6, 18:3n-3, and n-3HUFA for 9 weeks. The best growth and feed efficiency were obtained from the fish fed diet containing n-3HUFA (P<0.05). 2. n-3HUFA requirement Requirement of dietary n-3HUFA for the Korean rockfish (5.9 g in mean body weight) was investigated with the test diets containing different levels of n-3HUFA ranging from $0\%$ to $4.0\%$ at $8\%$ dietary lipid level. After 6 weeks of feeding experiment, fish performance and fatty acid composition of liver were studied. Growth was significantly improved with increasing dietary n-3HUFA level up to the $0.9\%$ in the diet (P<0.05). Higher values of lipid content, 18:1/n-3HUFA ratio of polar lipid of liver and hepatosomatic index were observed in the fish fed n-3HUFA deficient diets. The groups of fish fed lower levels of dietary n-3HUFA showed higher 18:1 and love. n-3HUFA (EPA+DHA) levels in polar lipid of the liver. The data obtained in these experiments indicated that dietary n-3HUFA was essential for the Korean rockfish, and required level of n-3HUFA was around $0.9\%$ in diet.

기존학교 시설개선사업의 통합적 추진을 통한 효율화 방안 -K-TEGS 평가기법을 통한-

이상민,김성중,맹준호,Lee, Sang-Min,Kim, Seong-Joong,Meang, Joon-Ho 한국교육녹색환경연구원 2011 교육·녹색환경연구 Vol.10 No.1

The importance of education and training facilities and education facilities on the relationship between effective as demonstrated in many studies can be very big. In this study, the status of existing school facilities improvement project is done individually, based on the systematic integration of the improvement project was to evaluate the feasibility. Furthermore, it was based on looking for ways to effectively pursue. Training School Facilities Improvement Program to be competitive, the country continued to be pursued systematically and very large scale as a whole must be a great national project. So much to promote efficient business environment, quality of education for children with detractors of the national budget to ensure efficient and most importantly in terms of economic investment that can be. In this study, the proposed K-TEGS existing school facilities, such as the development of techniques for the assessment based on this effort to promote an efficient business should be done on a continuous basis.

몰수평판에 의한 폰툰형 부유체에 작용하는 파랑강제력의 감소현상에 관한 연구

이상민,이원우,Lee, Sang-Min,Lee, Won-Woo 해양환경안전학회 2007 해양환경안전학회지 Vol.13 No.3

Pontoon type very large floating structure has been considered and actively studied as one of the most important ocean space utilization. The hydroelastic displacement of the pontoon type floating structure in waves is the largest at its weather side. The purpose of this study is to investigate the characteristics and effects of the submerged horizontal plate which is developed to reduce the wave exciting forces acting on the pontoon type floating structure using numerical analysis. The numerical method based on the finite difference method has been adopted and compared with the experimental data to confirm the reliability of it. We have performed the numerical computation of wave exciting forces acting on the pontoon type floating structure with/without submerged plate and discuss the results of simulation. 폰툰형 초대형 부체구조물은 해양공간의 활용에 있어서 가장 많이 고려되고 또한 적극적으로 연구되어지는 분야이다. 폰툰형 부체구조물의 해상에서의 유탄성 변형은 입사하는 파와 접하는 풍상측에서 가장 큰 값을 나타내고 있다. 본 연구에서는 폰툰형 부유체에 작용하는 파랑강제력을 감소시키고자 개발된 부유식 수평 몰수평판의 효과를 수치해석적인 방법에 의하여 그 특성을 파악하고자 한다. 유한차분법을 토대로 한 수치계산법을 적용하고, 적용된 수치계산법을 실험값과 비교함으로서 프로그램의 신뢰성을 확인한다. 검증된 수치계산법을 이용하여 본 연구에서 실시하고자 하는 폰툰형 부유체에 작용하는 파랑강제력의 계산을 수행하고 그 결과에 대하여 논의한다.