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박효진,김인수,김진우,양슬기,김민지,구덕본 사단법인 한국동물생명공학회 2019 한국동물생명공학회지 Vol.34 No.3
Sestrin-2 (SESN2) as a stress-metabolic protein is known for its anti-oxidative effects as a downstream factor of PERK pathways in mammalian cells. However, the expression patterns of SESN2 in conjunction with the UPR signaling against to ER stress on porcine oocyte maturation in vitro, have not been reported. Therefore, we confirmed the expression pattern of SESN2 protein, for which to examine the relationship between PERK signaling and SESN2 in porcine oocyte during IVM. We investigated the SESN2 expression patterns using Western blot analysis in denuded oocytes (DOs), cumulus cells (CCs), and cumulus-oocyte complexes (COCs) at 22 and 44 h of IVM. As expected, the SESN2 protein level significantly increased (p < 0.01) in porcine COCs during 44 h of IVM. We investigated the meiotic maturation after applying ER stress inhibitor in various concentration (50, 100 and 200 μM) of tauroursodeoxycholic acid (TUDCA). We confirmed significant increase (p < 0.05) of meiotic maturation rate in TUDCA 200 μM treated COCs for 44 h of IVM. Finally, we confirmed the protein level of SESN2 and meiotic maturation via regulating ER-stress by only tunicamycin (Tm), only TUDCA, and Tm + TUDCA treatment in porcine COCs. As a result, treatment of the TUDCA following Tm pre-treatment reduced SESN2 protein level in porcine COCs. In addition, SESN2 protein level significantly reduced in only TUDCA treated porcine COCs. Our results suggest that the SESN2 expression is related to the stress mediator response to ER stress through the PERK signaling pathways in porcine oocyte maturation.
A-14 : Preparation and charaterization of chitosan-based superporous semi-IPN hydrogels
박효진,김덕준 한국화학공학회 2007 화학공학의이론과응용 Vol.10 No.2
The purpose of this study was to develop chitosan-based porous semi-interpenetrating polymer network(semi-IPN) for gastric retention device. Chitosan and glycol chitosan hydrogels with numerous pores were prepared, and their swelling behaviors in acidic solution were studied. And, to improve mechanical strength of hydrogels, PEO and PVA were used in IPN synthesis with glycol chitosan. Swelling studies were performed in different pH solution at 37°C. Morphology and pore size of hydrogels were confirmed by scanning electron microscopy(SEM). Mechanical strengths were determined by UTM.
박효진,강진경,박인서,문영명,정재복,이용찬,김범수 대한소화기학회 1998 대한소화기학회지 Vol.31 No.4
Background/Aims: Seropositivity to CagA and/or VacA has been implicated in the pathogenesis of peptic ulcers. Korea is well known with high incidence of gastric cancer and Helicobacter pylori infection. However, most studies to evaluate CagA and VacA phenotype in Korea have been performed in limited cases and have showed inconsistent results. Some of these differences may be explained by variation in methods, geographic factors and socioeconomic factors. We evaluated the prevalence of seropositivity to CagA and VacA in different disease entities by using a commercial Western blot kit (Helicoblot 2.0^(TM), Genelabs Diagnostics, Singapore). This kit allows rapid visualization of full serologic profiles of various antigens which belong to H. pylori and standardization for international cornparisons. Methods: For 117 consecutive patients (72 nonulcer dyspepsia, 23 gastric ulcers and 22 duodenal ulcers, histology, rapid urease test (CLO^(TM) Delta- West, Western Australia) and ELISA test (GAP^(TM) test, BIORAD, Millan, Italy) were performed. From each patient, serum was collected and western blot assay was performed. Patients are considered as H. pylori positive if two of above tests were positive. Results: CagA and VacA were positive in 88.5% and 50.0% of H. pylori infected patients. All the VacA positive patients expressed CagA positivity while only 56.5% of CagA positive patients showed VacA positivity. Reactivities to CagA and VacA were not significantly different according to different types of disease. Comparison of western blot assay with ELISA test showed decrease in specificity of both tests after the age of 50 (76.0% vs 42.9%, 80.0% vs 42.9% respectively, p$lt;0.05). Conclusions: Reactivity of the H. pylori positive sera to western blot assay differs according to disease entities. Serologic profiles to CagA and VacA show no significant association with peptic ulceration in Korean patients with H. pylori infection. In addition, the western blot assay shows poor and limited specificity in old patients.