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점증적인 도전 신체활동이 아동의 건강체력 및 신체조성에 미치는 영향
박용선,김지수,이승범 한국웰니스학회 2011 한국웰니스학회지 Vol.6 No.1
The purpose of this paper is to provide by investigating the impact of their physical fitness and??body composition, by comparing normal weight children and Obese Children in the elementary school The Progressive Challenging Physical Activity Program was conducted with background music applied to both groups. Eight weeks later, 5 children and 4 Children. Utilizing SPSS, The progressive exercise program were measured and analyzed repeatedly with the data collected through the activities. The following is a summary of conclusions: First, The result of the eight weeks' the performance of the Physical Activity Program had a positive impact to improve physical fitness for both groups. In particular, physical fitness(cardiorespiratory endurance, muscle endurance, power) was significantly increased in both groups, obese children compared to the normal-weight children had a little??noticeable change, but there was no significant difference between the two groups. Second, The progressive Challenging Physical Activity Program had significant and positive effects on the body composition of both groups including weight, body fat, body fat amount, lean body mass, centeral obesity rate and muscle amount, but the difference between the two groups was not statistically significant.
Analysis of coenzyme Q10 in human plasma by high performance liquid chromatography
박용선,박상범,송선미,김용우,이경률 한국분석과학회 2009 분석과학 Vol.22 No.6
Coenzyme Q10(CoQ10), a vitamin E-like substance, represents a components of the complex antioxidant system of the human organism. CoQ10 levels in human plasma were determined by high performance liquid chromatography (HPLC) with UV detection. It was dissociated from lipoproteins by methanol and extracted into n-hexane with liquid-liquid extraction procedure, after centrifugation, the supernatant was dried under nitrogen gas stream. The residue was dissolved in the absolute ethanol. Determination of CoQ10 was performed on a C18 reversed-phase analytical column with ultraviolet detection at 275 nm and the mobile phase containing 15% (v/v) ethanol in methanol at a flow rate of 1.7 mL/min. The low limit of quantitation was 0.02 mg/L (S/N=10), the linearity between the concentration and peak height is from 0.1 to 2.0 mg/L. Twenty-four randomly selected plasma samples from apparently healthy, 27 to 44 year old individuals (males and females) were analyzed for total CoQ10. The average level in these subjects was 0.62±0.13 mg/L with the range of 0.41- 0.98 mg/L. This method has a specific and a sufficient limit of quantitation (LOQ) for analysis of CoQ10 in human plasma in both a clinical study and research at laboratories