흰쥐 대동맥에서 Trazodone의 혈관이완 작용기전

김상진,김정곤,김진상,Kim, Shang-jin,Kim, Jeong-gon,Kim, Jin-shang 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.4

The aim of this study was to investigate trazodone's effect on vasorelaxation and blood pressure lowering and to examine its underlying mechanism of action in isolated thoracic aorta and anesthesized rats. Precontracted aortic rings with high KCl were relaxed with trazodone, at concentrations of $50{\mu}M$ or greater. However, precontracted rings with phenylephrine (PE) were relaxed with trazodone, at concentrations of $0.03{\mu}M$ or greater, in a concentration-dependent manner. These relaxant effects of trazodone on endothelium intact rat aortic rings were significantly greater than those on denuded rings. The trazodone-induced relaxations were suppressed by nitric oxide synthase (NOS) inhibitors, N(G)-nitro-L-arginine (L-NNA) and N(omega)-nitro-L-arginine methyl ester (L-NAME), guanylate cyclase inhibitors, methylene blue and 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), a $Ca^{2+}$-activated $K^+$ channel blocker, tetrabutylammonium (TBA), a $Ca^{2+}$ channel blocker, nifedipine, $Na^+$ channel blockers, lidocaine and procaine, and removal of extracellular $Na^+$, but not by aminoguanidine, 2-nitro-4-carboxyphenyl-n, n-diphenylcarbamate (NCDC), indomethacin, glibenclamide and clotrimazole. In vivo, infusion of trazodone elicited significant decrease in arterial blood pressure. Trazodone-induced decrease in blood pressure was markedly inhibited by pretreatment of intravenous injection of saponin, L-NNA, methylene blue, TBA, lidocaine or nifedipine. These findings suggest that the endothelium-dependent relaxation and decrease in blood pressure induced by trazodone is mediated by release of NO from the endothelium, activation of TBA-sensitive $Ca^{2+}$-activated $K^+$ channels or inhibition of $Ca^{2+}$ entry through voltage-gated channel.

흰쥐에서 혈관내피 의존적인 혈관이완과 혈압하강에 대한 propofol의 억제 효과

김상진,김정곤,조성건,강형섭,김진상,Kim, Shang-Jin,Kim, Jeong-gon,Joe, Sung-gun,Kang, Hyung-sub,Kim, Jin-shang 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.3

We studied the effect of propofol (PPF) on the endothelium-dependent vascular responses in isolated rat thoracic aorta. In aortic rings with endothelium, PPF inhibited the phenylephrine (PE)-induced contraction in a concentration-dependent manner. In PE-precontracted preparations, PPF attenuated the endothelium-dependent relaxation by acetylcholine but not by A23187. And PPF did not attenuate the endothelium-independent relaxation by sodium nitroprusside (SNP). The relaxation induced by acetylcholine in PE-precontracted aortic rings was significantly augmented by zaprinast, a cGMP-specific phosphodiesterase inhibitor, and this augmentation was inhibited by PPF. Although SNP-induced relaxation was significantly augmented by zaprinast, this augmentation was not inhibited by PPF. In preparations preconstricted with PE, the PPF-induced relaxation was inhibited by atropine. In addition, PPF attenuated the vasorelaxation by phosphodiesterase inhibitors (IBMX, Ro20-1724 or zaprinast except milrinone). In vivo, the infusion of acetylcholine and SNP showed decreased arterial blood pressure in rats. The pre-injection of PPF inhibited the acetylcholine-induced blood pressure lowering, but not the SNP-induced blood pressure lowering. These results suggest that PPF can attenuate in part the acetylcholine-induced vasorelaxation and blood pressure lowering through the inhibition of the acetylcholine receptor-mediated endothelium-derived relaxing factor by acting on endothelium. It is considered that the inhibitory effect of PPF on the vasorelaxation is due to the decreased level of cGMP which can be attributed to the inhibition of the muscarinic receptor and/or receptor-G-protein interaction.

Effect of Mesoporous TiO₂ in Facilitated Olefin Transport Membranes Containing Ag Nanoparticles

김상진,정정표,김동준,김종학,Kim, Sang Jin,Jung, Jung Pyu,Kim, Dong Jun,Kim, Jong Hak The Membrane Society of Korea 2015 멤브레인 Vol.25 No.5

용액-확산 메커니즘에 의해 결정되는 기존의 고분자에서와는 달리, 촉진수송은 투과도와 선택도를 동시에 향상시킬 수 있는 기술이다. 본 연구에서는 은 나노입자, 폴리비닐피롤리돈, 7,7,8,8-테트라시야노퀴노디메탄으로 구성된 촉진수송 올레핀 분리막에 있어서, 메조기공 티타늄산화물($m-TiO_2$)에 대한 영향을 연구하였다. 특히 메조기공 티타늄산화물은 폴리비닐클로라이드-g-폴리옥시에틸렌 메타크릴레이트 가지형 공중합체를 템플레이트로 하여 쉽고 대량 생산이 가능한 방법으로 제조하였다. 엑스레이 회절분석에 따르면, 제조된 메조기공 티타늄산화물은 아나타제와 루타일 상의 혼합으로 구성되어 있으며, 결정의 크기가 약 16 nm 정도 되었다. 메조기공 티타늄산화물을 첨가하였을 때, 분리막의 확산도가 증가하여 혼합기체 투과도가 1.6에서 16 GPU로 증가하였고 선택도는 45에서 37로 약간 감소하였다. 메조기공 티타늄산화물이 첨가되지 않은 분리막은 장시간 성능이 유지되었으나, 메조기공 티타늄산화물이 첨가된 분리막의 경우 시간이 지남에 따라 투과도와 선택도가 감소하였다. 이는 티타늄산화물과 은 사이의 화학적 상호작용으로 은 나노입자의 올레핀 운반체로써의 활성을 감소시키기 때문으로 사료된다. Facilitated transport is considered to be a possible solution to simultaneously improve permeability and selectivity, which is challenging in normal polymeric membranes based on solution-diffusion transport only. We investigated the effect of adding mesoporous $TiO_2$ ($m-TiO_2$) upon the separation performance of facilitated olefin transport membranes comprising poly(vinyl pyrrolidone), Ag nanoparticles, and 7,7,8,8-tetracyanoquinodimethane as the polymer matrix, olefin carrier, and electron acceptor, respectively. In particular, $m-TiO_2$ was prepared by means of a facile, mass-producible method using poly(vinyl chloride)-g-poly(oxyethylene methacrylate) graft copolymer as the template. The crystal phase of $m-TiO_2$ consisted of an anatase/rutile mixture, of crystallite size approximately 16 nm as determined by X-ray diffraction. The introduction of $m-TiO_2$ increased the membrane diffusivity, thereby increasing the mixed-gas permeance from 1.6 to 16.0 GPU ($1GPU=10^{-6}cm^3$(STP)/($s{\times}cm^2{\times}cmHg$), and slightly decreased the propylene/propane selectivity from 45 to 37. However, both the mixed-gas permeance and selectivity of the membrane containing $m-TiO_2$ rapidly decreased over time, whereas the membrane without $m-TiO_2$ had more stable long-term performance. This difference might be attributed to specific chemical interactions between $TiO_2$ and Ag nanoparticles, causing Ag to lose activity as an olefin carrier.

흉부 방사선 영상의 정점영역 매칭을 통한 허파영역 자동검출에 관한 연구

김상진,김용만,이명호,Kim, Sang-jin,Kim, Yong-Man,Lee, Myoung-Ho 대한의용생체공학회 1990 의공학회지 Vol.11 No.2

This paper presents a new algorithm that extracted lung region in X-ray and enhanced the region. With a lung region that was extracted by histogram threshold value, it was diffi cult to detect perfect lung boundary. Therefore we presented perfect lung boundary detection method using apex detection and apex region restoration. Also, by applying modified equalization algorithm and presented function to inside of lung region, we want to give help to automatic diagnosis In X-ray chest image. Presented main line trace algorithm gave good result in detection of lung boundary And, as apex detection method using lung row and column gray level average value found more correct place of lung than the rpethod of prior algorithm, we succeeded perfect lung region detection, Also, presented function that had lung region's gray level distribution characteristic was very effective to image enhancement.

Exponential Nozzle을 사용한 Water Jet 발생에 관한 연구

김상진,윤의수,최태민,Kim, Sang-Jin,Yun, Ui-Su,Choe, Tae-Min 한국기계연구원 1990 기계연구원소보 Vol.20 No.-

Most water cannon machines use compressed gas to accelerate a piston which extrudes water through a cumulation nozzle. Very high jet stagnation-pressures can be achieved by using a specially-shaped nozzle which is initially filled with air or vacuum. The objective of this study was to establish the basic technology of water cannon using exponential type nozzle. An experimental water cannon including high pressure components such as exponential nozzle and 300atm air resrvior were designed and tested. Parameters that influence the performance of the system and jet characteristics were examined.

미국.일본의 심해생명공학 연구동향

김상진,Kim, Sang-Jin 한국미생물학회 2003 微生物과 産業 Vol.29 No.2

동기 및 비동기 겸용모드의 멀티미디어 원격교육 시스템 개발에 관한 연구

김상진,김석수,박길철,황대준,Kim, Sang-Jin,Kim, Seok-Soo,Park, Gil-Cheol,Hwang, Dae-Joon 한국정보처리학회 1997 정보처리논문지 Vol.4 No.12

본 논문에서 제안하는 원격교육 시스템은 정보 통신 기술과 멀티미디어 기술을 이용해서 사이버 스페이스상에서 열린 교육을 위한 교육자와 피교육자간의 상호 참여를 통한 동기 및 비동기의 학습이 모두 가능하도록 개발된 원격 교육 시스템이다. 특히 기존의 원격 교육 시스템이 갖는 생소함과 불편함을 가능한 느끼지 않도록 개발하였고, 면대면 효과를 최대로 지원하는 멀티미디어 기술상의 상호작용이 가능한 실시간 동기모드의 학습모드와 사용자 위주의 비동기 모드의 자율학습을 진행하다가 필요한 자료의 지원을 받아 원하는 선생님의 학습자료를 다운로딩 할 수 있고, 선생님과의 동기 학습 모드 변환이 가능한 시스템이다. 본 시스템은 응용 공유기술, 화이트보드, 다양한 비디오 윈도우 표시, 비디오, 사용자 인터페이스를 지원하고 있으며, 또한 세션관리, 통신제어, 각 미디어 제어의 제어 기술이 지원되고 있다. In this paper, we proposed the "synchronous & asynchronous distance education system" which is able to interact among teachers and students for open education in cyberspace, and it is based telecommunication technology and multimedia technology. Specially, This system gets rid of the nufamiliarity and inconvenient feeling during the distance education. Also it supports the mediation of floor mode, for a group lecture and supports the synchronous mode for face-to-face effective and asynchronous mode for self-learning. The asynchronous mode has the down load function and the consultant mode (between teacher and student). The element technologies of this system consists of application sharing technique, whiteboard, various video window display, audio support, user interface, environment setup, session management, access control, network control and media control for collaborative distance education.

다공성 폴리에틸렌 안와 삽입물의 피복 재료로서 신전된 폴리테트라플루오로에틸렌의 이용

김상진,곽상인,정호경-,Sang Jin Kim,Sang In Khwarg,Ho Kyung Choung 대한안과학회 2007 대한안과학회지 Vol.48 No.1

Purpose: To investigate the possibility of expanded polytetrafluoroethylene (e-PTFE) as a wrapping material for porous polyethylene orbital implant. Methods: Two groups of 6 female New Zealand albino rabbits underwent enucleation with placement of a 12 mm porous polyethylene orbital implant wrapped in simple e-PTFE in one group (group 1) and macro-porous e-PTFE in the other group (group 2). Histopathologic sections of the implants obtained at 4, 6, 8 weeks were compared on the extent of fibrovascular ingrowth, percentage of the area of proliferated vessel, and grade of fibrosis between the two groups. Results: There was no implant exposure or severe inflammation in group 1 and 2 during the observation. The extent of fibrovascular ingrowth differs only at 4 weeks. Group 1 showed less fibrovascular ingrowth (until outer 1/3) at anterior half compared with posterior one, but there was no difference between anterior and posterior half in group 2 (until middle 1/3). The percentage of the area of the proliferated vessels was higher in group 2 than group 1 at both anterior and posterior half, and the anterior/posterior ratio of the area of vessels was also higher in group 2 than group 1. The grade of fibrosis was higher in group 2 than group 1 at 4, 6, and 8 weeks. Conclusions: Macro-porous e-PTFE almost didn`t disturb fibrovascular ingrowth into the orbital implant, so macro-porous e-PTFE can be used safely as a wrapping material for porous polyethylene orbital implant.

계지반하생강탕 및 침구치료로 호전된 기능성 소화불량 환자의 치험 1례

김상진,이재홍,고석재,박재우,Kim, Sang-jin,Lee, Jae-hong,Ko, Seok-jae,Park, Jae-woo 대한한방내과학회 2016 大韓韓方內科學會誌 Vol.37 No.2

Objective: This case presents the effects of Gyejibanhasaenggang-tang, acupuncture, and moxibustion on functional dyspepsia.Method: A patient was treated with herbal medicine, acupuncture, and moxibustion. Functional dyspepsia-related quality of life (FD-QoL), the gastrointestinal symptom rating scale (GSRS), and the gastrointestinal scale (GIS) were assessed for evaluation.Results: Symptoms of functional dyspepsia improved after the 22 days of Gyejibanhasaenggang-tang treatment.Conclusion: This case suggests that the combination of Gyejibanhasaenggang-tang, acupuncture, and moxibustion could be effective in treating functional dyspepsia.

수치해석 모델링을 이용한 교차 흐름 미세유체 액적 생성 디바이스 채널 교차각이 액적 직경에 미치는 영향

김상진,강형섭,양영석,김기범,Kim, Shang-Jin,Kang, Hyung-Sub,Yang, Yeong-Seok,Kim, Gi-Beum 대한의용생체공학회 2015 의공학회지 Vol.36 No.3

In this paper, we studied the effects of intersection angles of the flow-foucusing type droplet generation device inlet channel on droplet diameter using numerical simulation modeling. We modeled different intersection angles with a fixed continuous channel width, dispersed channels width, orifices width, and expansion channels width. Numerical simulations were performed using COMSOL Multiphysics$^{(R)}$ to solve the incompressible Navier-Stokes equations for a two-phase flow in various flow-focusing geometries. Modeling results showed that an increase of the intersection angle causes an increase in the modification of the dispersed flow rate ($v^{\prime}{_d}$), and the increase of the modification of the continuous flow rate ($v^{\prime}{_c}$) obstructs the dispersed phase fluid flow, thereby reducing the droplet diameter. However, the droplet diameter did not decrease, even when the intersection angle increased. The droplet diameter decreased when the intersection angle was less than $90^{\circ}$, increased at an intersection angle of $90^{\circ}$, and decreased when the intersection angle was more than $90^{\circ}$. Furthermore, when the intermediate energy deceased, there was a decrease in the droplet diameter when the intersection angle increased. Therefore, variations in the droplet diameter can be used to change the intersection angle and fluid flow rate.