자생 및 재배 큰비쑥의 이화학적 특성 비교

강보라(Bo Ra Kang),김은희(Eun Hee Kim),김연경(Yeon Kyoung Kim),김아현(Ah Hhyun Kim),오규연(Gyu Yeon Oh),박유민(Yoo Min Park),조아현(Ah Hyeon Jo),나환식(Hwan Sik Na),신미영(Mi Yeong Shin),안양준(Yang Joon An),조정용(Jeong Yong Cho),이소 한국식품저장유통학회 2024 한국식품저장유통학회지 Vol.31 No.2

국화과에 속하는 큰비쑥은 ‘갯쑥’으로도 알려져 있는 염생식물로서 생물학적 이용 가능성이 높은 2차 대사산물이 풍부할 것으로 기대되며, 항염, 항산화, 미백효과 등 다양한 생리 활성이 있다고 알려져 있다. 따라서 본 연구에서는 큰비쑥을 경쟁력 있는 작물로써 이용성을 높이고자 생장 환경별 총폴리페놀 함량, 라디칼 소거능, 무기질 함량, 중금속 및 잔류농약을 분석하였다. 총폴리페놀은 가장 높은 함량을 보인 자생 성숙잎을 제외하고는 유의적인 차이가 없었으나(p<0.05), ABTS 및 DPPH 라디칼 소거능은 자생잎보다 재배잎에서 높은 활성을 보였다. 이는 폴리페놀 화합물의 구조적 요인이나 폴리페놀 화합물 이외의 영양성분들이 라디칼 소거능에 복합적으로 작용한 결과로 판단된다. 무기질 중 Na 함량은 자생 어린잎이 가장 높았으나, Na을 제외한 총 무기질 함량은 재배잎이 자생잎보다 높았다. K 및 Ca 함량은 자생잎보다 재배잎이 높았다. 큰비쑥 4종의 안전성을 알아보기 위해 중금속을 분석한 결과, Pb, Cd, As, Hg이 검출되었으나, 엽채류 식약처 고시 기준인 Pb 0.3 mg/kg, Cd 0.2 mg/kg보다 낮은 수준으로 나타났고 전반적으로 재배잎이 자생잎보다 낮게 측정되었다. 잔류농약은 큰비쑥 4종에서 검출되지 않아 안전하다고 판단된다. 결론적으로 본 연구를 통해 재배 큰비쑥은 전라남도 해안지역 자생 큰비쑥과 비교하여 큰 차이가 없어 재배잎을 육종하여 대량생산한다면 이용 가능성이 높은 품종으로 활용 가능하다고 판단된다. Artemisia fukudo Makino belongs to the Asteraceae family, is a halophyte that can grow in salinity soils and is known for its various physiological activities. However, few studies were comparing it according to the growth environment. Therefore, in this study, we analyzed the antioxidant activity, functional ingredients, and safety of Artemisia fukudo Makino according to the growth environment. Total polyphenol content was the highest in native mature leaves, but 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity was higher in cultivated leaves than in native leaves. The total mineral content, excluding sodium, was higher in cultivated than in native leaves. The content of potassium and calcium was higher in cultivated leaves than in native leaves. In addition, heavy metal analysis showed that cultivated leaves were generally lower than those of native leaves. Residual pesticides were not detected in all samples. In conclusion, since there is no significant difference in cultivated leaves compared to native leaves, it was judged that cultivated leaves could be used as a variety to be grown and mass-produced.

영구치의 선천적 결손을 동반한 복합 치아종

김하나,김재곤,백병주,한지혜,양연미 大韓小兒齒科學會 2007 大韓小兒齒科學會誌 Vol.34 No.4

치아종은 치아조직의 비정상적인 과성장으로인해 형성되는 외배엽성 상피세포와 중배엽세포로 구성된 혼합종양이다. 양성 치성 종양 중에서 가장 흔하며 신생물이라기보다 과오종으로 보고 있다. 치아종은 악골내 모든 부위에서 발생할 수 있지만 치아와 유사한 복합 치아종은 비교적 상악 전치부에 호발하고,불규칙한 형태를 나타내는 복잡 치아종은 하악 구치부에 호발한다. 일반적인 증상이 없어 일상적인 방사선 검사에서 주로 발견되며 영구치의 맹출지연이나 매복,유치의 만기잔존 등의 원인이 될 수 있지만 영구치의 선천적 결손을 동반한 증례는 매우 드물다 치료는 외과적 제거가 추천되며 완전한 적출시 재발은 드물다. 본 증례는 영구치의 선천적 결손을 동반한 복합 치아종 환자에 대하여 보고하는 바이다. Odontoma is the most common benign odontogenic tumors, and have been defined as mixed odontogenic tumor composed of epithelial and mesenchymal cells. Odontoma is believed to be hamartomatous rather than neoplastic in nature. The classification by WHO divides odontoma into 2 groups such as complex odontoma and compound odontoma. Compound odontoma comprises dental tissues, resembling the morphology of a tooth and has predilection for the anterior maxilla, In contrast, complex odontoma has unorganized mass, not resembling the normal tooth and has predilection for the posterior mandible. Odontoma is almost asymptomatic, so it is usually found on routine radiographic examination. Common presenting symptom is impacted or unerupted permanent teeth and retained primary teeth, but coexistent odontoma and congenital missing of permanent teeth is a very rare condition. The recommended treatment for an odontoma is conservative surgical excision, with care taken to remove the surrounding soft tissue. This report presents 2 patients with compound odontoma of the mandible who have congenital missing of the permanent teeth.

선천성 결손치에 관한 임상적 연구

정해경,양연미,김재곤,백병주,정진우,김하나,김미아 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.2

The congenital missing of teeth is common, which takes place since the proliferation and differentiation are not allowed in that tooth bud fail to start development. The purpose of this study is to research incidence rate, number, and missing part of congenital missing teeth, and to study whether a person who has missing teeth has other abnormality of teeth or not. For this study, 1,520 subjects(aged 2.9~17) who had visited pediatric dentist department of Chonbuk national university dental hospital within 2 years were examined with an panoramic radiograph ; exempting third molar missing state. The obtained results are as follows. 1. 8.88% among total subjects show missing teeth ; male 9.05%, female 8.64% 2. The most frequently missing permanent teeth were the mandibular second premolars(22.3%). The most frequently missing primary teeth are mandibular lateral incisors(50%). 3. 43.3% patients have one permanent missing tooth, 34.3% have two, and 10.4% have more than six, respectively. In primary teeth, 86.7% patients have one missing tooth, and 13.3% have two missing teeth. 4. 18 patients(13.3%) have missing teeth as well as hyperdontia, while some patients have microdont, ectopic eruption, and fusion teeth. 치아의 선천성 결손은 치배의 발육이 시작되지 못하여 치아의 증식, 분화가 일어나지 못함으로서 발생하는 흔한 치아의 발육 이상이다. 이 연구의 목적은 선천성 결손치의 발생률, 결손치의 수, 발생부위와 결손치 환자에게서 나타난 다른 치아이상의 발생여부를 조사하는 것이다. 본 연구는 2006년 7월부터 2008년 6월까지 전북대학교 소아치과에 내원하여 파노라마를 촬영한 1,520명의 환자(2.9~17세)를 대상으로 제 3 대구치를 제외한 선천성 결손치의 분포를 조사하여 다음과 같은 결과를 얻었다. 1. 전체 1,520명 중 총 8.88%에서 결손치가 관찰되었으며, 남자가 9.05%, 여자가 8.64%에서 결손치가 관찰되었다. 2. 총 350개의 영구 결손치 중 하악 제 2 소구치가 22.3%로 가장 발생률이 높았고, 하악 측절치, 상악 제 2 소구치, 하악 중절치, 상악 측절치 순이었다. 또한 총 18개의 유치 결손치 중 하악 유측절치가 가장 높은 발생률(50%)을 보였다. 3. 1개의 영구치 결손치를 가지는 환자는 43.3%, 2개는 34.3%, 3개는 6,7%, 4개는 1.5%, 5개는 3.7%, 6개 이상은 10.4%이었다. 또한 유치 결손치의 수는 1개는 86.7%, 2개는 13.3%이었다. 4. 결손치가 있는 환자 중 과잉치를 가지는 환자는 18명(13.3%)이 있었으며, 왜소치, 이소맹출, 융합치 등의 치아이상을 지닌 환자도 관찰되었다.

시판되는 생수 내 무기물 함량에 관한 연구

소유려,백병주,김재곤,양연미,김하나 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.3

생수 내에는 많은 미네랄이 포함하고 있으며, 이 중 칼슘, 나트륨, 칼륨, 마그네슘, 불소는 생수통에 반드시 표기해야 할 무기물이다. 칼슘, 마그네슘, 불소와 같은 무기물은 치아형성에 관여하며, 적절하게 섭취시 치아우식증을 예방할 수 있다. 현행 먹는 샘물 수질기준에 따르면 무해무기물질인 칼슘과 마그네슘에 대한 기준치는 없으며, 유해무기물질인 불소와 같은 무기물은 2 mgF/L 이하로 규정하고 있다. 본 연구는 국내에서 판매되고 있는 생수 15종을 대상으로 칼슘, 마그네슘, 불소의 농도를 측정하였고, 생수 내 무기물 함량의 표기 여부 및 무기물 농도를 비교, 검토하여 다음과 같은 결론을 얻었다. 1. 15개의 생수 중 1개의 생수를 제외하고 모두 칼슘함량을 표기하였다. 평균 칼슘농도는 34.68±31.84 mg/L, 최대 12.891±1.85 mg/L, 최소 2.0±0.02 mg/L이었다. 2. 15개의 생수 중 2개의 생수를 제외하고 모두 마그네슘 함량을 표기하였다. 평균 마그네슘 농도는 9.22±11.06 mg/L, 최대 30.43±0.75 mg/L, 최소 0.0 mg/L이었다. 3. 15개의 생수 중 4개의 생수를 제외하고 모두 불소 함량을 표기하였다. 평균 불소 농도는 0.25±0.33 mg/L, 최대 1.13±0.04 mg/L, 최소 0.01±0.03 mg/L이었다. 모두 생수는 현행 먹는 샘물 기준치인 2 mgF/L 이하에는 만족시켰다. Drinking water has lots of minerals, especially calcium, sodium, kalium, magnesium, and fluoride must be labelled on the bottle about their contents. Minerals like calcium, magnesium, and fluoride have influence to the tooth development. Appropriately taking some minerals, dental caries can be prevented somewhat degree. There is no guide line about innoxious minerals like calcium and magnesium, however, noxious mineral like fluoride, should be contained less than 2 mgF/L according to the current drinking water standard. Hereupon, it is necessary to recognize the concentration of fluoride in drinking water, so I studied 15 samples of domestic drinking water on sale about the concentration of calcium and magnesium, fluoride. The results obtained were as follows ; 1. 14 drinking waters in 15 samples showed various Ca concentration. The average Ca concentration is 34.68±31.84 mg/L. the highest is 128.91±1.85 mg/L and the lowest is 2.0±0.02 mg/L. 2. 13 drinking waters in 15 samples indicate the Mg concentration. The average concentration is 9.22±11.06 mg/L. the highest is 30.43±0.75 mg/L and the lowest is 0.0 mg/L. 3. 11 drinking waters in 15 samples indicate the F concentration. The average concentration is 0.25±0.33 mg/L, the highest is 1.13±0.04 mg/L and the lowest is 0.01±0.03 mg/L. All samples are satisfied the current drinking water standard, 2 mg F/L.

대학교 대형 강의실의 보행장애인 접근성을 위한 베리어프리 디자인 적용 실태

양한나(Han na Yang),이두나(Doo na Lee),연소라(So ra Yeon),이현정(Hyun Jeong Lee) 충북대학교 생활과학연구소 2016 생활과학연구논총 Vol.20 No.1

Accessibility to education spaces including college lecture rooms is directly related to right to education of persons with disabilities. This study aimed to investigate barrier-free design application in large lecture rooms in colleges focusing on accessibility of persons with mobility impairments. Total 83 lecture rooms accommodating 80 or more persons in two universities in Chungcheong Province were selected as study targets. Field survey using a barrier-free design checklist was conducted between March 19, 2015, to April 3, 2015. Major findings were as follows.(1) Application rate of items in lecture room entrance section were higher than that of items in in-room passage and assigned seat sections. (2) To compare characteristics between items that showed high application rates and low application rates, application rates of items related to building structure such as level difference and width of entrance and passage were found relatively satisfactory while items that could be easily improved with low cost and efforts such as height of entrance handle, closing time of a door check, wall safety bar, anti-sliding treatment on ramp, assigned seat for persons with disabilities were not applied well. (3) Among items with low application rates, ramp needs to be adopted from planning stage as it is not easy to be added after construction completion to secure proper gradient for wheelchair users (1/12 or less). (4) When planning seats for wheelchair users, adaptable seats in diverse locations are desirable so that the users could have location choices.

증례 : 현미경적 다발성 혈관염 환자에서 폐색전증으로 오인되었던 Swyer-James 증후군

박지나 ( Jee Na Park ),나승연 ( Seung Yeon Na ),이연아 ( Yeon Ah Lee ),이상훈 ( Sang Hoon Lee ),홍승재 ( Seung Jae Hong ),양형인 ( Hyung In Yang ) 대한내과학회 2007 대한내과학회지 Vol.73 No.1S

도토리묵 제조용 조전분의 원산지별 성분 및 항산화 특성

양기현(Kee-Heun Yang),안장혁(Jang-Hyuk Ahn),김효진(Hyo-Jin Kim),이지연(Ji-Yeon Lee),류보람(Bo-Ram You),송정은(Jung-Eun Song),오혜림(Hye-Lim Oh),김나연(Na-Yeon Kim),김미리(Mee-Ree Kim) 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.7

도토리묵 제조용 조전분의 품질 특성을 규명하기 위한 기초연구 목적으로 수입 원산지(국내산, 북한산, 중국산)별로 일반성분, pH 및 산도, 무기질 및 당 조성, 색도, 총 페놀함량, 항산화 활성 등을 조사하였다. 일반성분에서는 북한산의 경우 수분함량은 적고 탄수화물, 조지방, 조단백이 국내산이나 중국산보다 높았다. pH는 3개 시료에서 유사하였으며 산도는 북한산이 0.021%로 중국산이나 국내산보다 다소높게 나타났다. 이러한 산도의 유의성 차이는 채취년도 및 보관온도, 보관기간 등에 의한 수분량 감소, 성분변화 등에 기인한 것으로 생각된다. 특히 원료별 유통경로 및 보관온도, 보관기간에 차이가 있었다. 무기질의 경우 국내산은 Na 함량이 적고 (4.52 ㎎/100g), Mg(14.11 ㎎/100g)과 Ca(136.84 ㎎/100g) 함량이 높았으며, 북한산은 K(94.87 ㎎/100g)과 P(18.17 ㎎/100g)이, 중국산은 Fe(4.55 ㎎/100g)과 Zn(1.07 ㎎/100g)이 상대적으로 높았다. 당 성분의 경우 포도당 함량은 국내산(187 ㎎/100g)이, 자당은 중국산(80 ㎎/100g)이 높았다. 색도에서는 명도값(L)이 북한산에서 59.89로 가장 낮은 값을 보였으며, 국내산 64.60, 중국산 64.48으로 차이를 보였다. 적색도(a)와 황색도(b)에서는 북한산이 2.55, 13.15로 가장 낮은 값을 보인 반면, 국내산과 중국산은 유의적인 차이가 없었다. 총 페놀 함량에서는 북한산이 0.009 ㎎/㎖로 가장 높았으며 다음으로 중국산 0.006 ㎎/㎖, 국내산 0.004 ㎎/㎖ 순으로 나타났다. DPPH radical 소거능의 경우 IC?? 값에서 북한산이 47.0 ㎎/㎖로 가장 높았고 중국산 73.7 ㎎/㎖, 국내산 86.8 ㎎/㎖로 차이를 보였다. 또한 hydroxyl radical 소거능에서도 북한산의 IC?? 값이 0.038 ㎎/㎖로 가장 높은 활성을 나타냈으며, 그 다음으로 중국산 0.041 ㎎/㎖, 국내산 0.044 ㎎/㎖ 순이었다. 따라서 이들 수입 원산지별 도토리묵 제조용 조전분에 대한 분석 결과 성분별 함량 차이는 존재하였으며 어느 시료에서 전체 품질이 우수하다고 보기는 어려웠다. 그러므로 현재 수입되고 있는 북한산 중국산 도토리묵 제조용 조전분에 대한 원산지별 다른 영양성분을 고려하여 생산하고자 하는 특정 가공식품의 이용을 검토하여 그 특성에 맞게 적용하려는 노력이 필요할 것이다. Nutritional compositions of acorn crude starch were analyzed according to country of origin, especially the mineral and sugar contents. Regarding the three kinds of starch of domestic (South Korea, KAS), Chinese (CAS), and North Korea (NAS) origins in the Korean market, NAS had the lowest moisture content as well as the highest contents of crude protein, crude fat, and carbohydrate. Regarding mineral contents, NAS contained the highest amounts of magnesium, calcium, sodium, potassium, and phosphorous, whereas CAS contained the highest iron and zinc contents. There were no significant differences in acidity and pH. Analysis of the monosaccharide contents of the starches showed that glucose was the highest in KAS while sucrose was the highest in CAS. The Hunter color L value was the lowest in NAS, whereas a and b values were the lowest in CAS. Total phenolic content was the highest in NAS. NAS had the highest DPPH and hydroxyl radical scavenging activities (IC??: 47.0 ㎎/㎖ for DPPH, 0.038 ㎎/㎖ for hydroxyl) whereas KAS had similar DPPH (IC??: 73.7 ㎎/㎖ for CAS, 86.8 ㎎/㎖ for KAS) and hydroxyl radical activities (IC??: 0.041 ㎎/㎖ for CAS, 0.044 ㎎/㎖ for KAS) as compared to CAS.

Quantitative Validation and Analysis of Ginsenosides Composition for Manufacture of Standardized Ginseng Products

Yu ju Lee,Kyung Soon Kim,Min Ah Park,Na Ryeong Kim,Na Young Jang,Soo Yeon Cho,Na Gyeom Kim,Byung Oh Lee,Jun Ho Yang,Byung Wook Yang 한국산업식품공학회 2019 학술대회 및 심포지엄 Vol.2019 No.10

Analysis of DNA repair mutation in Lynch syndrome-associated endometrial carcinoma in Korea

( Yeon-joon Jeong ),( Eun-ju Lee ),( Gwang-jun Kim ),( Ha-na Park ),( Shin-ho Yang ),( Bo-ra Lee ),( Ji-hye Kim ) 대한산부인과학회 2018 대한산부인과학회 학술대회 Vol.104 No.-

Objective: Lynch syndrome, as known as hereditary non-polyposis colorectal cancer, is an autosomal dominant inherited disease by germline mutations in DNA repair genes including MLH1, MSH2, MSH6, PMS2 and EPCAM. In Lynch syndrome, endometrial cancer is the second most common cancer. High microsatellite instability and mismatch repair deficiency, which are characteristic of lynch syndrome, are one of the molecular-based classification of endometrial cancer. Endometrial cancer with lynch syndrome has become more important with the advent of FDA-approved Pembrolizumab in these traits. However, the national prevalence of germline mutation in DNA repair genes in Korean women with Lynch syndrome remains unknown. The aim of the study is to find most common mutation of DNA repair genes in Korean women with Lynch syndrome-associated endometrial carcinoma. Methods: Ten reports of Korean endometrial cancer patients who confirmed lynch syndrome and three patients who were detected at Chung-Ang University Hospital were reviewed. Age, the family history based on Amsterdam criteria II, immunohistochemical analysis, germline mutation, microsatellite instability status, FIGO stage, grade, and type were collected. Descriptive statistical analysis was used. Results: A total 28 cases were summarized. Eighteen (64.3%) cases met Amsterdam criteria II for Lynch syndrome. Mean age at onset of endometrial cancer was 49.0 years (range 27-60 years). Immunohistochemical analysis was reported in 20 patients and losses of MLH1, MSH2, MSH6, or PMS2 proteins were observed in endometrial cancer tissues. The germline mutations of MLH1, MSH2 and MSH6 were found in 15 (53.6%), 9 (32.1%) and 4 (14.3%), respectively. Microsatellite instability was assessed in ten patients and nine patients had high microsatellite instability. Conclusion: MLH1 is most common germline mutation and its mutations were located in ten sites which could be novel in Korean women with Lynch syndrome-associated endometrial carcinoma.

Proteomic analysis of methamphetamine-induced reinforcement processes within the mesolimbic dopamine system

Yang, Moon Hee,Kim, Seyoon,Jung, Min-Suk,Shim, Jung Hee,Ryu, Na Kyung,Yook, Yeon Joo,Jang, Choon-Gon,Bahk, Young Yil,Kim, Kee-Won,Park, Jong Hoon Wiley (Blackwell Publishing) 2008 Addiction biology Vol.13 No.3

<P>ABSTRACT Methamphetamine (MAP) is a commonly used, addictive drug, and a powerful stimulant that dramatically affects the central nervous system. In this study, we used the conditioned place preference (CPP) paradigm in order to study the reinforcing properties of MAP and the herewith associated changes in proteins within the mesolimbic dopamine system. A CPP was induced by MAP after three intermittent intraperitoneal injections (1 mg/kg) in rats and protein profiles in the nucleus accumbens, striatum, prefrontal cortex, cingulate cortex and hippocampus were compared with a saline-treated control group. In addition, a group of animals was run through extinction and protein profiles were compared with a non-extinguished group. Protein screening was conducted using two-dimensional electrophoresis analysis which identified 27 proteins in the group that showed MAP-induced CPP. Some of the proteins were confirmed by Western lot analysis. Identified proteins had functions related to the cytoskeleton, transport/endocytosis or exocytosis (e.g. profilin-2 and syntaxin-binding protein), and signal transduction, among others.</P>