HPLC를 사용한 쌀 중 잔류농약 동시분석법

최재천,이영자,김소희,최수영,최희주,정성욱,박흥재,김우성 한국환경과학회 2005 한국환경과학회지 Vol.14 No.4

A simple and sensitive analytical method based on RP-HPLC with UV detector(225 nm) and mobile phases using 0.1% phosphoric acid and acetonitrile was developed for simultaneous determination of quinclorac, bentazone, 2,4-D, bensulfuron-methyl, dymuron, capropamide, pencycuron, ethofenprox. This method was resulted in recovery of 78~ 96% with RSD 3.3~7.5%. LODs 0.12~0.84 and LOQs 0.34~1.20 ㎎/L. Calibration curves were linear with r of 0.9995~ 0.9999.

전국 10개 종합병원 중환자실 환자의 직장내 Vancomycin-resistant Enterococci(VRE) 보균율, 보균의 위험요인 및 유전적 다양성 조사

정희진,송준영,엄중식,김우주,최선주,최정현,이혁,정문현,이광미,신동현,손보라,최종성,우흥정,권정아,이경원,최강원,박승철 대한감염학회 2002 감염 Vol.34 No.5

프로토콜 분석을 통한 창의적 디자인 전략으로서의 은유와 유추

최한희(Choi, Han_Hee),주서령(Ju, Seo Ryeung),김미정(Kim, Mi Jeong) 한국실내디자인학회 2013 한국실내디자인학회논문집 Vol.22 No.1

The aim of this research is to explore the potential of analogy and metaphor as design strategies for supporting the creative design thinking. For the empirical research on analogical and metaphoric process in designing, we conducted design experiments that consist of three different conditions of given items as stimulation for design inspiration: baseline, surrealist paintings, and housing design collections. The results were analyzed using a protocol analysis in order to obtain more systmatic interpretation of the design processes and strategies. As a result, it was noted that students are more apt to read visual information rather than semantic information in the given items. Instead of the representation of their senses or feelings from the paintings, they visualized the analogical images of the paintings for the design representation. However, analogical and metaphoric thinking derived from the given items seem change a designer’s perspective, thus bring a novel interpretation on design problems, and eventually more creative and meaningful design ideas. An extended research using one-semester training and observation of the design studio process is introduced as a follow-up study for this paper. This research will investigate the long-term effect of the analogy and metaphor on the design thinking.

아버지의 양육 참여행동과 아동의 친사회적 도덕판단능력

정현희(Hyean Hee Jeong),최경순(Kyoung Soon Choi) 한국아동학회 1992 아동학회지 Vol.13 No.1

The purposes of this study are to investigate (1) the father`s child-rearing behavior perceived by children in relation to children`s sex, family type and mother`s job. (2) children`s prosocial moral judgement as related to children`s sex, family type, and mother`s job, and (3) the effect of fathers child-rearing behavior on children`s prosocial moral judgement. The subjects were 432 5th-6th grade elementary school children in Pusan. For the measurement of father`s child-rearing behavior, this researcher developed a father`s child-rearing behavior questionnaire based on Choi`s (1991) questionnaire. For the children`s prosocial moral judgement, the 4 prosocial moral dilemma by Eisenberg-Berg was used. The major findings were as follows. No significant results were found in relation to father`s child-rearing behavior and child`s sex, family type, and mother`s work status. Children`s prosocial behavior was not related to any of the above variables. Significant relationships were found between father`s child-rearing behavior and children`s prosocial moral judgement.

혈액 투석 환자에서 중심정맥 협착에 대한 스텐트 삽입술 : Wallstent Placement

임대승,노상필,이유선,정승현,김보영,이정우,강정아,김정희,이민수,정준용,최시완,정진옥,성인환,이강욱,신영태 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.1

Stenosis of central vein is a common complication arising after percutaneous subclavian vein catheter insertion performed for temporary vascular access in chronic renal failure patients undergoing hemodialysis. There are several treatment methods for the condition like percutaneous angioplasty(PTA), stent insertion, and surgery, but recent trend is toward PTA and stents. Among the patients diagnosed with chronic renal failure from March 1993 to May 2002 and undergoing hemodialysis through AV fistula, the 14 Patients in whom central vein stenosis arose were selected for the study. A total of 28 percutaneous interventions(5 PTA and 23 stent placement) were performed, and restenosis rate and the time taken till the restenosis in de novo lesions and instant lesions were compared. All 28 cases were operated successfully. The 14 cases that received both anigioplasty and stent placement initially. (de novo lesion : 14 cases), Among the 10 cases with de novo lesion that followed up more than 1 year, 3 cases are currently undergoing hemodialysis without restenosis, and the remaining 7 cases have recurred stenosis with the mean time to restenosis of 10.9 months. In the 7 cases in whom stenosis recurred, 11 interventions were done(instent lesion: 11 cases). 4 of these were using only ballon angioplasty with 100% restenosis rate and the mean time of 3 months until restenosis. The remaining 7 cases were using both balloon angioplasty and stent placement, also with 100% restenosis rate but with the mean time of 12 months until restenosis, which was later than the group receiving only balloon angioplasty. In treating the patients with central vein stenosis, stent placement seems to be more advantageous over PTA in terms of restenosis rate and the mean duration of patency. In the case of instent lesion, inserting the stent for the second time after stenosis recurred lengthened the duration of patency compared to performing balloon angioplasty alone.

환경친화성 계면활성제를 이용한 어병균의 살균효과

최상원,정관용,오남희,김은영,방정환,김정우,여문환 한국환경과학회 2000 한국환경과학회지 Vol.9 No.5

The antibacterial effect amino acid-copper(Ⅱ) surfactant on fish pathogens was studied. Fish pathogens of Edwardsiella tarda, Vibrio anguillarum, Aeromonas hydrophila and Streptococcus sp. were selected, cultured in nutrient agar and adjusted at 2 × 10 exp (5)∼10 exp (6) CFU/㎖ in phosphate buffer saline before the addtion of amino acid-copper(Ⅱ) surfactant with different concentrations. All tested pathogens died within 1 hour with 1 ppm of amino acid-copper(Ⅱ) surfactant. In comparison with formalin and ET, amino acid-copper(Ⅱ) surfactnat was more effective in antibacterial capacity.

유아에서 발생한 재발성 외음부 콘딜로마 1례의 치료

홍정희,문성택,김윤숙,전섭,최승도,선우재근,배동한 순천향의학연구소 2007 Journal of Soonchunhyang Medical Science Vol.13 No.2

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CASE 프로그램에 의한 중학생들의 인지가속 효과

최병순,한효순,강성주,이상권,강순희,박종윤,남정희 한국과학교육학회 2002 한국과학교육학회지 Vol.22 No.4

학생들의 인지발달을 촉진시켜 학습효과를 높이려고 개발된 CASE 프로그램의 효과를 조사했다. 중학교 1학년(841명) 학생들이 연구에 참가하였으며, 같은 분량의 시간동안 실험반에서는 교육과정에 따른 과학 교과 수업과 '생각하는 과학' 활동을 병행하였고, 통제반에서는 과학교과 수업만을 수행하였다. 연구 초기에 논리적 사고력 검사(SRT II)를 이용하여 집단의 동질성을 비교하고, 2년 동안 실험반에 CASE프로그램을 처치한 후 이질동형겸사도구인 SRT VII을 이용하여 처치별, 성별, 인지수준별 인지가속에 대한 효과를 조사했다. CASE 프로그램에 의한 중학생들의 인지발달 가속효과는 중간검사에서는 보이지 않았으나, 사후검사에서 상당히 큰 것으로 확인되었다. 성별에 따라 CASE 프로그램의 효과를 비교한 결과 남??여 중학생들 모두에게 유의미한 인지가속이 이루어진 것으로 나타났다. 남학생들 보다 여학생들의 인지수준이 더 발달된 것으로 밝혀졌으며, 처치와 성별간 상호작용 효과는 나타나지 않았다. 학생들의 사전 인지수준에 따라 CASE 프로그램의 효과를 분석한 결과, 모든 인지수준에서 처치효과가 뚜렷하게 있었으나 특별한 경향성은 나타나지 않았다. 특히 초기 구체적 조작기(ES=1.1.)와 전조작기(ES=1.07), 그리고 구체적조작 후기(ES=1.01) 학생들에게 상당히 큰 효과가 있었다. 이상과 같은 CASE 프로그램의 성공적인 결과는 현행 우리나라 과학교육과정 개정과 교수방법 개선에 시사하는 바가 많다. In an attempt to accelerate the development of formal reasoning ability of students, 'Thinking Science' activities developed by the Cognitive Acceleration through Science Education(CASE) project were implemented to 841 students in 7th grade aged 12+ in six middle schools over a period of two years. Homogeneity between the CASE group and control group was tested with SRT Ⅱ, while the improvement of formal reasoning ability of the students was tested with SRT Ⅶ. The results were analyzed by treatment, gender, and cognitive levels of the students. Statistically significant gains were shown in the CASE group compared with those in the control group. Cognitive level of girls in the CASE group significantly increased as compared with the control group, while there was moderate effect in boys. These results implied that the thinking science activities were effective in cognitive acceleration of girls aged 12+. It was shown that much more CASE students in pre or concrete operational level shifted. to formal operational level as compared with the control group while there were significant effects in all levels (ES=0.31∼1.10) without showing any tendency.

CRP* 의존성 maltose 대사 촉진 유전자 sfs4의 클로닝 및 염기배열 결정

최용락,정희태,조무제,정수열 東亞大學校附設遺傳工學硏究所 1996 遺傳工學硏究 Vol.- No.3

CRP (cAMP receptor protein)은 cAMP와 결합하여 cAMP-CRP 복합체를 형성하여 전사조절의 조절인자로서 작용한다. crp 유전자에 변이를 도입하여 cAMP의 비존재 상태에서 cAMP-CRP와 비슷한 기능을 가진 crp* 유전자가 도입된 대장균 MK2001 (crp*¹, cya::km)을 숙주로 사용하여 cAMP 혹은 cGMP의 비존재하에서도 mal 유전자의 발현을 촉진시키는 유전자 sfs (sugar fermentation stimulation) 수 종을 클로닝하였다. 본 실험에서는 이미 밝혀진 nlp (Ner like protein) 유전자와 같이, sfs의 새로운 유전자를 탐색하여, 그 중 sfs4의 2126bp 전 염기배열을 결정하고, 잠정적인 sfs4의 promoter 영역에는 CRP 단백질과의 결합 DNA 공통 염기배열(5' AAT TGTGA ACACCA TCACC CGT 3')이 존재함을 확인했다. lacZ 융합 유전자를 작성하여 TP2010R1과 MK2001의 균주에서 cAMP를 첨가할 경우 각각 2.3배, 1.8배의 β-galactosidase 활성이 증가 하는 것으로 보아 sfs4는 cAMP-CRP에 의해 발현 조절을 받는 것으로 나타났다 In Escherichia coli, CRP forms a complex with cAMP and acts as a transcriptional regulator of many genes, including sugar metabolism operons. The E. coli MK2001, which is introduced the altered crp*¹, is functional in the expression of lac, ara and man, in the absence of cAMP. However, the expression of mal gene is fully activated by the addition of cAMP or cGMP. The object of the study is cloning of the sfs (sugar fermentation stimulation) genes, which was involved in regulation of mal gene expression with the altered crp*¹gene, and structural analysis and characterization of the genes at the molecular level. We have cloned 5 different E. coli genes which stimulate the maltose metabolism in a crp*¹, cya::km (MK2001) background. Newly identified genes were designated as sfs. One of the sfs genes (pPC1), located at the 53.2 min map position in the E. coli chromosome, was further analyzed. Expression of the genes, which is involved in maltose metabolism, malQ (amylomaltase), was increased to 5.8-fold in the presence of a plasmid, pAP5, containing the subcloned sfs4 gene. The nucleotide sequence of a common 2,126bp segment of the pPCM1 was determined and two open reading frames (ORF1 and ORF2) were detected. The ORF1 encodes the sfs4 gene and ORF2 encodes a truncated protein. Potential CRP binding site is located in the upstream of the putative promoter in the regulatory region. Expression of the cloned sfs4 gene was positively regulated by the cAMP-CRP complex.

호흡율 측정기를 이용한 폐수의 외부탄소원 이용가능성 평가

최은희,고주형,임정훈,부경민,김창원 부산대학교 환경문제연구소 2000 環境硏究報 Vol.18 No.-

In this research, we measured Readily Biodegradable COD(Ss) using respiration meter which measured OUR and calculated respiration rate due to calculate organic matter portion of external carbon sources because known organic matter analysis methods i.e. COD, BOD did not measure amount of organic matter which was used in denitrification. Based on the experimental results, leather wastewater had 40.3% Ss in TCOD and food fermentation liquid, alcoholic wastewater and petrochemical wastewater was 38%, 38% and 28.3% respectively. However in case of considering of flowrate and concentration, flood fermentation and alcoholic wastewater was revealed that they were effective external carbon sources