The Action Mechanism and Reaction of Hormone

정준용 단국대학교 자연과학대학 생물학과 1990 단국 Bio Vol.4 No.-

한국인에서 Lipoprotein lipase gene polymorphism의 빈도와 혈관질환과의 관계

정준용,박소영,김제,성인환,김영건,전은석 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2

Background : Lipoprotein lipase(LPL) is a key enzyme in the metabolism of serum triglycerides(TG) which is utilized in the peripheral tissue as free fatty acid and stored in adipose tissue. LPL gene is consisted of 10 exons which encode 475 amino acids and over 9 LPL gene polymorphisms have been reported. It's polymorphisms are related to the lipid levels and the severity of atherosclerosis in the coronary artery disease. In Korea, LPL polymorphisms has not been reported yet. This study is purposed on the incidences of LPL gene mutations and on it's relationships with blood lipid levels and the severity of atherosclerosis. Methods : Our subjects were consisted of three groups, normal controls(n=50), coronary artery disease(CAD, n=51) and cerebrovascular disease(CVD, n=52). The PCR amplified genomic DNA from peripheral white blood cell were analyzed with restriction fragment length polymorphisms (RFLP by two different restriction enzyme(PVU II, Hind III). Results : Total cholesterol(TC) were higher in CVD than in controls and in CAD (203±60mg/ dl vs 188±37, 167±42, p<0.01). Triglyceride(TG) were also elevated in CAD(166±65mg/dl vs 122±62 in controls, p<0.05). HDL cholesterol(HDL-C) were higher in controls than in CVD and CAD(49±9mg/dl vs 36±10, 44±9, p<0.05). The incidences of Hind III RFLP and PVU II RFLP has no differences among groups. Only HDL-C was elevated in Hind III RFLP(-/-) homozygotes. Conclusions : The LPL gene mutations in intron 6 and 8 have no direct effects on the lipid profiles and the severity of coronary artery disease. Although LPL is a key enzyme in TG metabolism, two mutations in this study could not change the activity of LPL, nor were not a marker of linked other site mutations. The mutation(s) in exon which encode amino acid for enzyme activity should be detected to analyze the pathophysiology of the atherogenesis.

Photo-functional electro-optic polyimide with excellent high temperature stability

정준용,서해민,가재원,임종선,원종찬,김경옥,이미혜 한국고분자학회 2018 한국고분자학회 학술대회 연구논문 초록집 Vol.43 No.1

Electro-optic polyimides containing disulfide group with improved poling property

정준용,서해민,가재원,임종선,김동균,김경옥,이미혜 한국고분자학회 2018 한국고분자학회 학술대회 연구논문 초록집 Vol.43 No.1

Real-Time RT-PCR 기법을 이용한 사이토카인 유전자 발현의 정량분석

정준용 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.2

The analysis of cytokine profiles plays a central part in the characterization of disease-related inflammatory pathways and the identification of functional properties of immune cells, both for research and for clinical diagnosis. Because tissue biopsy samples are too small to allow the detection of cytokine protein, the detection of mRNA expression level by reverse transcription-polymerase chain reaction (RT-PCR) analysis is often used to investigate the cytokine profiles in inflammatory lesions. RT-PCR itself is a qualitative method, indicating the presence or absence of specific mRNA. The most widely accepted method is qualitative competitive RT-PCR, based on internal shortened standards. Recently, real-time RT-PCR has been introduced for quantitative analysis of cytokine expression profiles. The real-time RT-PCR is becoming widely used to quantify cytokines from cells, tissues, tissue biopsies or body fluids. Being a very powerful and sensitive method it can be used to quantify mRNA expression levels of cytokines, which are often very low in the tissues under investigation. The method allows for the direct detection of PCR product during the exponential phases of the reaction, combining amplification and detection in one single step. In the review, I discussed the principle of real-time RT-PCR, the different methodologies and chemistries available, the assets, and some of pitfalls. Also, validation for a large panel of human cytokines and other factors playing a role in the immune system is discussed in detail. In summary, the real-time RT-PCR technique is very accurate and sensitive, allows a high throughput, and can be performed on very small samples.

변이형 협심증에서 추적 관상동맥 조영술을 통한관상동맥 연축에 대한 연구

정준용,임대승,강정아,이민수,김정희,송인관,최시완,정진옥,성인환 대한심장학회 2002 Korean Circulation Journal Vol.32 No.9

조리과정에 따른 콩나물 중의 수은잔유량

정준용,박정덕,정규철,Chung, Jun-Yong,Park, Jung-Duck,Chung, Kyou-Chull 대한예방의학회 1986 Journal of Preventive Medicine and Public Health Vol.19 No.2

This study was carried out in order to estimate the residual amount of mercury in soy-bean sprouts in each steps of cooking. Samples were taken at markets and also cultured at home without applying the mercury containing pesticides as control. Mercury was determined by dithizone method. It was disclosed that soy-bean sprouts purchased at markets contained $1.32{\pm}0.274ppm$, 13 times as high as the maximal allowable concentration of mercury in food recommeded by Ministry of Health and Social Affairs. Mercury contents, however, dropped off steadily by steps of cooking: rinsed with distilled water and boiled in distilled water showing concentrations of $0.11{\pm}0.025ppm$ in boiled sprouts and $0.03{\pm}0.022ppm$ in sprout-soup. These values were not statistically different from those in control samples, and not exceeded the maximal allowabled levels of mercury in food. It can be concluded that the use of mercury containing pesticides in the cultivation of soy-bean sprouts is not so serious problem as it has been suspected in respect of food contamination, but careful attention must be paid to indiscriminate use of mercury containing pesticides as they may contaminate air, water and soil and secondarily bring harm to human health through food chains.

블록체인을 활용한 영업비밀의 보호방안 연구: 영업비밀 원본증명서비스를 중심으로

정준용,김정규,이환수 한국산업보안연구학회 2019 한국산업보안연구 Vol.9 No.2

Trade secret is the most essential technology for corporate's survival. And this protection of trade secret is an important factor in the development of the corporate. So there are several laws and systems for protecting trade secrets. Among them, the trade secret original certification service confirms the existence of original and the time of possession of trade secrets (electronic document) through its unique electronic fingerprint (hash value) extracted from the trade secret and time information from the public certification authority. The advantage of this service is that it made without details of the corporate's trade secret, thereby reducing the burden on the corporate and making it affordable. However, If a leak of trade secret occurs, it is only a means of proving the possession time of the trade secret. And electronic document contents which electronic fingerprint of the trade secret original certification service is extracted will only presuming that it is consistent with the corporate’s trade secret contents. These problems will eventually act as a limit of the deduction ability. Therefore, in this study, we tried to identify and solve the limitations of the current trade secret original certification service by utilizing Blockchain. Specifically, we proposed a service management method through a public authority organization by storing the trade secret in a block like ‘the Private Blockchain’ and ‘the Consortium Blockchain’ among the types of blockchains. This makes it possible to reduce the burden of proof responsibility on the trade secret. Furthermore, It could be used as a means of protecting the trade secret by utilizing the advantages of the blockchain. 영업비밀은 기업의 생존에 가장 핵심적인 원천 기술로서 기업의 발전을 위해서는 영업비밀의 보호가 중요하다. 영업비밀의 보호를 위해 여러 법률 및 제도가 존재하고 있으며 그 중 영업비밀 원본증명서비스는 영업비밀에서 추출한 고유 전자지문(Hash값)과 공인인증기관의 시간 정보를 통해 영업비밀(전자문서)의 원본 존재 여부와 보유 시점을 확인해주고 있다. 또한 본 서비스는 기업의 영업비밀의 내용을 제출하지 않고 이루어져 기업의 부담이 완화되고 저렴하게 이루어진다는 장점이 있다. 그러나 영업비밀의 유출이 발생하게 되면 영업비밀의 보유 시점을 입증하는 수단에 불과하고, 원본증명서비스의 전자지문이 추출된 전자문서에 기재 된내용이 기업의 영업비밀 내용과 일치한다고 추정하는 것에 그치게 되고 이는 결국추정력의 한계로 작용하게 된다. 따라서 본 연구에서 블록체인을 활용하여 현재의 영업비밀 원본증명서비스가 가지는 한계점을 파악하고 해결하고자 하였다. 구체적으로 블록체인의 종류 중 프라이빗 블록체인(Private Blockchain) 및 컨소시엄 블록체인(Consortium Blockchain) 활용하여 블록 내 영업비밀 내용을 저장하여 공신력 있는 기관에 의한 서비스 운용 방안을 제시하였다. 이를 통해 영업비밀 해당 여부의 입증 책임 부담을 덜어주고 나아가 블록체인이 가지는 장점을 활용하여 영업비밀 자체를 보호하는 수단으로서 블록체인을 활용할 수도 있게 된다.

지역정보와 색 정보의 단계적 적용에 의한 능동 객체 추적

정준용,이규원,Jeong, Joon-Yong,Lee, Kyu-Won 한국정보처리학회 2012 정보처리학회논문지B Vol.19 No.2

실시간으로 입력되는 영상으로부터 지역정보 및 색 정보의 단계적 적용에 의한 Pan, Tilt 카메라를 이용한 능동객체추적방법을 제안한다. 환경 잡음을 제거하기 위하여 가우시안 필터링으로 전처리를 행한다. 적응적인 가우시안 혼합 모델링을 이용하여 배경과 객체를 분리한다. 객체가 분할되면 객체의 중심에 가깝게 탐색 윈도우를 설정하고 객체의 색 정보를 추출한다. 추출된 색 정보를 카메라가 이동하는 동안에도 추적이 가능한 CAMShift 추적 알고리즘을 적용하여 객체를 실시간으로 추적한다. 추적에 있어서 객체의 중심점이 화면의 중앙에 놓이도록 Pan, Tilt를 제어함으로써 적합한 추적이 이루어지도록 한다. 실험을 통하여 제안한 방법이 기존의 수동 영역 설정 방법보다 효과적임을 보였다. An active object tracking algorithm using Pan and Tilt camera based in the stepwise application of region and color information from realtime image sequences is proposed. To reduce environment noises in input sequences, Gaussian filtering is performed first. An image is divided into background and objects by using the adaptive Gaussian mixture model. Once the target object is detected, an initial search window close to an object region is set up and color information is extracted from the region. We track moving objects in realtime by using the CAMShift algorithm which enables to trace objects in active camera with the color information. The proper tracking is accomplished by controlling the amount of pan and tilt to be placed the center position of object into the middle of field of view. The experimental results show that the proposed method is more effective than the hand-operated window method.

'몰랐다'가 통용되지 않는 전쟁터

정준용,Jeong, Jun-Yong 한국데이터베이스진흥원 2000 디지털콘텐츠 Vol.2 No.-

이미 우리가 사는 환경의 일부가 되어버린 인터넷. 때문에 새로운 시대를 예고하는 인터넷 문명 속에서 사회의 안정과 균형을 추구하는 추구하는 도구로서의 법의 역할은 장차 우리사회의 변화의 폭과 깊이를 결정짓는 주요한 척도가 될 듯 싶다. 어떻게 활용되고 대비를 해야하는지 살펴봤다.