Identification of Genes Modulated by High Extracellular Calcium in Coculture of Mouse Osteoblasts and Bone Marrow Cells by Oligo Chip Assay

Kim, Hyung-Keun,Song, Mina,Jun, ji-Hae,Woo, Kyung-Mi,Kim, Gwan-Shik,Baek, Jeong-Hwa The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Calcium concentration in the bone resorption lacunae is high and is in the mM concentration range. Both osteoblast and osteoclast have calcium sensing receptor in the cell surface, suggesting the regulatory role of high extracellular calcium in bone merabolism. In vitro, high extracellular calcium stimulated osteoclastogenesis in coculture of mouse osteoblasts and bone marrow cells. Therefore we examined the genes that were commonly regulated by both high extracellular calcium and 1,25(OH)_(2)vitaminD_(3)(VD3) by using mouse oligo 11 K gene chip. In the presence of 10 mM[Ca^(2+)]e or 10 nM VD3, mouse calvarial osteoblasts and bone marrow cells were co-cultured for 4 days when tartrate resistant acid phosphatase-positive multinucleated cells start to appear. Of 11,000 genes examined, the genes commonly regulated both by high extracellular calcium and by VD3 were as follows; 1) the expressions of genes which were osteoclast differentiation markers or were associated with osteoclastogenesis were up-regulated both by high extracellular calcium and by VD3; trap, mmp9, car2, ctsk, ckb, atp6b2, tm7sf4, rab7, 2) several chemokine and chemokine receptor genes such as sdf1, scya2, scyb5, scya6, scya8, scya9, and ccr1 were up-regulated both by high ectracellular calcium and by VD3, 3) the genes such as mmp1b, mmp3 and c3 which possibly stimulate bone resorption by osteoclast, were commonly up-regulated, 4) the gene such as c1q and msr2 which were related with macrophage function, were commonly down-regulated, 5) the genes which possibly stimulate osteoblast differentiation and/or mineralization of extracellular matrix, were commonly down-regulated;slc8a1, admr, plod2, lox, fosb, 6) the genes which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were commonly up-regulated;s100a4, npr3, mme, 7) the genes such as calponin 1 and tgfbi which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were up-regulated by high extracelluar calcium but were down-regulated by VD3. These results suggest that in coculture condition, both high extracellular calcium and VD3 commonly induce osteoclastogenesis but suppress osteoblast differentiation/mineralization by regulating the expression of related genes.

황남대총 98호분 출토 유리의 과학적 분석

조경미,유혜선,강형태 국립중앙박물관 1999 박물관보존과학 Vol.1 No.-

황남대총 98호분 북분과 남분에서 출토된 유리시료 40점에 대한 과학적인 분석을 실시하였다. 유리의 성분조성은 주사전자현미경-에너지분산형분광기(SEM-EDS)를 사용하여 정량분석 하였고 다변량해석법을 통하여 시료를 분류하였다. 그 결과 시료 40점 모두 Na₂O를 약 20%정도 함유한 소다-석회(Na₂O CaO-SiO₂)유리임을 확인하였으며, 다시 5개 주성분(SiO₂, AI₂O₃, Na₂O, CaO, K₂O)으로 다변량해석[주성분분석(PCA)]을 실시한 결과 2개의 群으로 분류되었다. I 群에 포함된 시료는 Al₂O₃의 농도가 9.7%로 높고 CaO의 농도는 2.2%인데 비하여 Ⅱ群에서는 각각 3.2%,4.9%의 범위로 나타났다. 특히 시료 No.12의 노란 색으로 편석된 부분을 미소부위 XRD로 분석한 결과 PbSnO₃임을 국내 최초로 확인할 수 있었다. 鉛을 함유한 시료 중 No.12, 17은 열이온화질량분석기(TIMS)로 납동위원소비를 측정하였고 선형판별식 분석법(SLDA)을 이용하여 납의 산지를 추정하였다. 그 결과 각각 중국 남부, 한국 남부의 납광석을 사용한 것으로 나타났다. Elemental analysis of 40 glass samples from the Northern Tomb and the Southern Tomb of Hwangnam-daechong No. 98 was performed. Fourteen compositions of each sample were analyzed quantitatively by SEM-EDS and glass samples were classified by multivariate analysis such as PCA. All of 40 samples were confirmed to be Na₂O CaO-SiO₂ system with about 20% of Na₂O. Samples were classified into two groups by doing PCA on concentrations of 5 major elements (SiO₂, AI₂O₃, Na₂O, CaO, K₂O). Samples included in group I showed the concentration of AI₂O₃ is about 9.7% and that of CaO, about 2.2%. In group II, concentration of Al₂O₃ is about 3.2% and that of CaO, about 4.9%. Especially yellow grains embedded in sample No.12 were shown to be PbSnO₃ by micro XRD, which was the first coloring material ever found in Korea. Lead isotope ratios of samples No.12 and No.17 which contained lead were measured by TIMS. The origin of lead was traced by means of multivariate analysis such as SLDA. The result showed that lead from southern China and southern Korea had been used for making glass.

중앙아시아 벽화 보존처리(1) : 벽화(본4074, 본4096)의 상태조사

강형태,이용희,유혜선,김연미,조연태,시고 아오키,노리코 야마모토,켄타로 오바야시 국립중앙박물관 2001 박물관보존과학 Vol.3 No.-

국립중앙박물관은 용산 새 박물관의 전시 유물로 선정된 중앙아시아 벽화의 연차적인 보존처리를 위해 “독립행정법인 일본 도쿄문화재연구소’’와 공동연구를 모색하게 되었다. 2001년에 본4074, 본4096 작은 벽화편 2점의 보존처리를 시작하면서 벽화의 균열 박락 등 손상상태, 구조와 구성재료, 이전 보존처리에서 적용된 재료와 방법에 대한 기초조사를 실시하였다. 조사결과 본4074, 본4096 2점의 벽화는 흙과 지푸라기를 반죽하여 만든 벽체에 석고를 발라 바탕으로 하고 그 위에 채색을 한 것으로 나타났으며 벽체 속에 포함된 지푸라기의 방사선 탄소연대측정 결과 본 벽화는 10세기 말에서 13세기 초에 만들어진 것으로 나타났다. 또한 X-선회절 분석 결과 화면의 흰색 바탕은 gypsum[Ca(SO₄)·2H₂O]과 CaSO₄, Calcite(CaCO₃)가 적색계통은 연단(鉛丹:Pb₃O₄)과 led arsenate[Pb(As₂O₆)], 녹색계통은 Cuprite(Cu₂O)와 arsenolite(As₂O₃), arsenic oxide(As₂O₄) 등이 사용된 것으로 조사되었다. This article is about a joint project carried out by the National Museum of Korea and the Tokyo Cultural Properties Research Institute for the conservation of central Asia Wall painting that has been selected for the exhibition at the new Seoul National Museum of Korea at Yongsan. The investigation of the wall painting revealed very useful information. This includes the condition of the object, and the identification of evident damage, such as cracks, loss of pigment, plus materials and methods employed during the object's creation, as well as previous conservation treatment. The object was mainly made by applying plaster to the body (wall) that consisted of a mixture of soils and rice straws. Then, on the surface of the wall-painting, pigments were used to draw and to colour it. As a part of the investigation, radiocarbon dating was conducted using straw samples taken from the object. The result indicates that the object is probably dated form between the end of the 10th Century and the beginning of the 13th Century. The result of X-ray diffraction also revealed the composition of the pigments used on the surface. These are 1. gypsom[Ca(SO₄)·2H₂O], CaSO₄ and Calcite(CaCO₃) and Calcite(CaCO₃) that were used for the white background. 2. Pb₃O₄ and led Arsenate [Pb(As₂O₆) that were used for the red colouring. 3. Cuprite(Cu₂O), Arsenolite(As₂O₃) and Arsenic Oxide(As₂O₄) that were used for the green colouring.

형광 in situ hybridization 방법에 의한 c-myc 유전자의 전위 및 증폭에 관한 연구

김경채,박선화,김강련,김미영,전용혁 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.3

정신분열증 환자에서 청각 인식유발전위와 임상적 호전의 연관성에 대한 예비연구

박미경,최영,이형영 대한신경정신의학회 1999 신경정신의학 Vol.38 No.1

연구목적 : 본 연구는 정신분열증 환자에게 치료에 대한 반응을 예측할 수 있는 객관적 지표를 알아보려는 노력으로 시행되었다. 방 법 : 연구대상은 정신과 진단편람 제 4권 (Diagnostic and Statistical Manual of Mental Disorders-Ⅳ)의 정신분열증 진단기준을 만족시키는 23명의 입원환자였다. 치료 전 또는 치료 초기에 청각 인식유발전위를 시행하였으며 임상증상의 변화는 치료 전과 치료 4주 후 양성 및 음성 증후군 척도(Positive and Negative Syndrome Scale)를 통하여 측정하였다. 결 과 : 1) 양성척도의 호전정도는 P1과 N1의 잠복기와 유의한 정 상관관계를 보이고, P3 잠복기, P2의 전위와는 유의한 역 상관관계에 있었다. 2) 음성척도의 호전정도는 P3 잠복기와 유의한 역 상관 관계를 보였다. 3) 일반정신병리척도의 호전도 또한 P3 잠복기와 유의한 역 상관관계를 보였다. 결 론 : 이러한 결과는 치료전 실시한 청각 인식유발인자가 정신분열증 환자에게 임상적 호전의 예측인자로서 가치가 있음을 시사한다. 향후 많은 환자를 대상으로 지속적인 연구가 필요하다. Objectives : The purpose of this study was to see the correlation between the auditory cognitive evoked potential and the clinical improvement in schizophrenics. Method : The subjects were 23 patients with schizophrenia. Auditory cognitive evoked potential was examined at pretreatment or acute phase of treatment. The change of symptom was assessed by the PANSS(Positive and Negative Syndrome Scale) before drug treatment and in the 4th week of treatment. Results : 1) The change of positive subsclae was positively correlated with P1 latency(p〈.05), N1 latency(p〈.05) and negatively correlated with P3 latency(p〈.05), P2 amplitude(p〈.05). 2) The change of negative subscale was negatively correlated with P3 Latency(p〈.05). 3) The change of general psychopathology subscale was negatively correlated with P3 latency(p〈.05). Conclusion : These results suggest that the auditory cognitive evoked potential in schizophrenic patients could be valuable on prediction of clinical improvement. But, further studies are required to support these results.

마약길항제의 방출 제어형 제제 (제2보) : 나록손 이식제제의 생체적합성 및 약물속도론적 평가 Biocompatibility and Pharmacokinetics of Naloxone Implant

문미란,박주애,이승진,김형국,김길수 梨花女子大學校 藥學硏究所 1995 藥學硏究論文集 Vol.- No.5

For the effective administration of narcotic antagonist, the application of sustained release implantable systems with biodegradable polyphosphazene was examined. Using poly[(diethyl glutamate)-co-(ethyl glycinate) phosphazene]. the implantable devices containing naloxone hydrochloride were prepared and in vivo implantation studies were carried out subcutaneously in rat and rabbit with this preparation for the biocompatibility and pharmacokinetics. The histological finding in rats at initial time period was the inflammation that occurred focally around the implants, but they were showed subsequent mild and limited chronic inflammations and the irreversible changes such as necrosis and degeneration of the muscle or connective tissues were not observed. Therefore the placebo and naloxone implants are considered to be biocompatible formulations histologically. In pharmacokinetic studies, the release of naloxone from the naloxone implants into blood plasma was maintained in 192 hours, but the initial burst effect was observed. If this problem was solved, the application for the narcotic antagonist sustained release systems can be expected.

정보처리유형에 따른 유아의 이야기 구성능력의 차이

오미형 한국아동교육학회 2005 아동교육 Vol.14 No.1

본 연구에서는 유아의 정보처리 양식에 따라 유아가 구성하는 이야기의 구조와 길이에 차이가 있는지를 살펴보고자 하였다. 연구의 대상을 표집하기 위해 한국판K-ABC 지능검사를 실시하여 유아의 정보처리 유형에 따라 동시처리형 11명과 순차처리형 15명을 무선 표집하였다. 이야기 구성 검사 도구로 글없는 그림책을 사용하였으며 이야기구성력과 이야기의 길이를 측정하였다. 이야기구조와 이야기길이를 종속변인으로 하고 유아의 정보처리양식을 독립변인으로 하여 MANOVA를 실시하였다. 연구결과 유아가 구성한 이01기의 구조와 길이는 유아의 정보처리 유형에 따라 차이가 있는 것으로 나타났다. The purpose of this study was to examine the differences of Story Construction according to children with a strength in sequential processing or simultaneous processing. For sampling the subject of this study, Korean version K-ABC Intelligence Test(Moon, Soo-Back, 1997)was conducted 96 children aged 5 who were attending B kindergarten in U city. As a result of this test, 15 children of sequential processing style and 15 children of simultaneous processing style were sampled randomly. As for test tool, 'story construction from a picture book' which was made by Kraayenoord & Paris, was modified, used. Data was analyzed by MANOVA according to problems. Followings are the results of this study: Firstly, there was no significant difference between sequential processing style and simultaneous processing style in 'metalinguistics' of story construction, but in cases of 'comments about pictures' and 'elaboration and personal involvement', the children group of sequential processing style got statistically higher grades than the children group of simultaneous processing style. And in cases of 'revision strategies' and 'theme/moral' of story construction, the children group of simultaneous processing style got statistically higher grades than the children group of sequential processing style. Secondly, there was significant differences between children's information processing style in story length. That is, the children group of sequential processing style made longer story than the children group of simultaneous processing style.

웹 어플리케이션 서버 기반의 인트라넷 시스템 설계 및 구현

이형묵,윤영미 京畿專門大學 1999 京畿專門大學 論文集 Vol.- No.27

Internet has been applied to information technology business and extends to the whole industrial world. As the most effective way of information propagation in an organization, implementing an intranet system makes a distinctive progress and various implementation methodology has been introduced. Our study proposed a solution for the intranet system commanding an university administrial and educational tasks making a comparison and an analysis among various intranet system implementation technologies, identified business requirements processes, implemented the intranet system based on this framework, and applied the system to our college.

랫드에서 인체 재조합 적혈구 조혈인자, rHuEPO의 13주 정맥투여 아만성독성에 관한 연구

김형식,곽승준,천선아,박현선,한하수,임소영,안미영,김원배,김병문,안병옥,홍성렬,이병무 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

A recombinant human erythropoietin (rHuEPO) was administered intravenously at dosage levels of 0, 100, 500, and 2500 IU/㎏/day for a period of 13 weeks. There were no observed clinical signs and deaths related to treatment in all groups tested. Decreases in body weight gain and food consumption were observed only in males of 2,500 IU/㎏ group after 2 weeks. In hematological parameters, erythrocyte content, hematocrit values and hemoglobin concentration were dose-dependently increased in rHuEPO treated groups. The ratio between kidney weight and whole body weight was significantly increased in females of 500 and 2,500 IU/㎏ groups. The spleen weight was also increased in both sexes of 500 and 2,500 IU/㎏ groups. However, the absolute weight change of other organs was not observed. In histopathological examinations, the renal tubular basophilia was observed only in males and females of 2,500 IU/㎏ groups. From these results, it is concluded that the no-observed adverse effect level(NOAEL) of rHuEPO is 100 IU/㎏ in rats in the present study.

Monocrotaline으로 유발시킨 흰쥐 폐고혈압에 대한 Flunarizine의 약리작용

안형수,이영미 同德女子大學校 1997 同大論叢 Vol.27 No.1

Monocrotaline treatment (60mg/kg, i.p.) in normotensive rats resulted the indications and related toxicities of pulmonary hypertension, including the increase of hematocrit value, right ventricle hypertropy, pulmonary edema and lethality, after 4 weeks later. However, daily administrtion of flunarizine (5mg/kg, p.o.) for 4 weeks seemed to decrease the above responxes, and also there was no significant change on the systemic mean arterial blood pressure and heart rate. In the anesthetized rats, hypotensive effect induced by isoproterenol bolus injection was signficantly inhibited in flunarizine-treated pulmonary hypertensive rats, comparing to the untreated rats, even if those by acetylcholine, adenosine and ATP were not. In the isolated pulmonary artery experiments, vasorelaxation induced by isoproterenol, but not acetylcholine, adenosine or ATP, were also selectively inhibited in flunarizine treated rats, comparing to the control rats. Also vasoconstriction induced by norepinephrine were increased in flunarizine treated rats comparing to the control rats, but those by KCI were not. However, pretreatment of flunarizine did not significantly affected on the vasodilation induced by acetylcholine, isoproterenol, adenosine or ATP and on the vasoconstriction induced by norepinephrine in isolated pulmonary artery of the normal rats. These results suggest that flunarizine will decrease the pathogenesis of pulmonary hypertension through the change of α- and β-adrenergic receptor activity in pulmonary artery due to the up-regulation of voltage dependent Ca++ channels.