Immunohistochemical study on the expression of calcium binding proteins (calbindin-D28k, calretinin, and parvalbumin) in the cerebral cortex and in the hippocampal region of nNOS knock-out(−/−) mice

Yu Jin Cho,Jae Chul Lee,Bong Gu Kang1,2,Jaeyeol An,Hyeon Suk Song1,2,Onju Son,Do-Hyun Nam,Choong Ik Cha,Kyeung Min Joo 대한해부학회 2011 Anatomy & Cell Biology Vol.44 No.2

Nitric oxide (NO) modulates the activities of various channels and receptors to participate in the regulation of neuronal intracellular Ca2+ levels. Ca2+ binding protein (CaBP) expression may also be altered by NO. Accordingly, we examined expression changes in calbindin-D28k, calretinin, and parvalbumin in the cerebral cortex and hippocampal region of neuronal NO synthase knockout(−/−) (nNOS−/−) mice using immunohistochemistry. For the first time, we demonstrate that the expression of CaBPs is specifically altered in the cerebral cortex and hippocampal region of nNOS−/− mice and that their expression changed according to neuronal type. As changes in CaBP expression can influence temporal and spatial intracellular Ca2+ levels, it appears that NO may be involved in various functions, such as modulating neuronal Ca2+ homeostasis, regulating synaptic transmission, and neuroprotection, by influencing the expression of CaBPs. Therefore, these results suggest another mechanism by which NO participates in the regulation of neuronal Ca2+ homeostasis. However, the exact mechanisms of this regulation and its functional significance require further investigation.

UL밴드에서 BT와 IEEE 802.11에 의한 간섭의 분석 연구

강석진(Suk-Jin Kang),차정근(Jung-Keun Cha),강형구(Hyung-Koo Kang),고진환(Jin-Hwan Koh) 한국정보과학회 2002 한국정보과학회 학술발표논문집 Vol.29 No.1A

요즘 사무 빌딩이나 공항에서의 ISM(Industrial Scientific and Medical) 밴드 사용이 증가함에 따라 동일 주파수 대역을 사용하는 BT(Bluetooth)와 무선 랜은 상호 충돌 발생이 일어난다. 이러한 문제점이 최근 연구과제로 등장하고 있다. 본 논문은 채널 간섭의 모델에 의거하는 UL(unlicensed) ISM 주파수 대역에서 BT와 802.11에 있어서 충돌 평가 모델에 의한 간섭을 분석 한다. 전파 평가 모델 방법론에 의한 AP와 STA간의 범위에 따른 BT 간섭의 수와 허용할 수 있는 최대 BT 신호를 알아본다. 그리고, BT를 바탕으로 한 액세스 네트워크의 실행에서 802.11b 시스템에 관한 BT의 기본 간섭 메커리즘 및 간섭이 어느 정도 미치는지에 관한 예비 결과를 나타낸다.

足三里 電鍼이 정상 白鼠 小腸運動에 미치는 영향

차숙(Suk Cha),박상무(Sang Moo Park),윤정안(Jeong Ahn Yun),유윤조(Yun Jo Yu),강병기(Byung Ki Kang),김강산(Kang San Kim) 한의병리학회 2006 동의생리병리학회지 Vol.20 No.4

Rapid identification of furanocoumarins in Angelica dahurica using the Online LC-MMR-MS and their nitric oxide inhibitory activity in RAW 264.7 cells

Kang, Suk Woo,Kim, Chul Young,Song, Dae-geun,Pan, Cheol-Ho,Cha, Kwang Hyun,Lee, Dong-Un,Um, Byung-Hun John Wiley Sons, Ltd. 2010 Phytochemical analysis Vol.21 No.4

<P>Introduction – Angelica dahurica (Fisch. Ex hoffm.) Benth. Et Hook. is a perennial herb that grows throughout Korea whose dried roots have been used to treat various diseases in Korean traditional medicine. The root extract contains diverse constituents, and it is necessary to determine the active compounds.</P><P>Objective – To investigate the nitric oxide (NO) inhibitory activity in a root extract of A. dahurica and identify the most active compounds using LC-NMR-MS.</P><P>Methodology – In search of the anti-inflammatory constituents of A. dahurica extract, the HPLC-based activity profiling approach was used to investigate the extract's NO inhibitory activity. To directly identify the compounds, a hyphenated LC-NMR-MS technique was applied. Reversed-phase isocratic chromatography was performed using the acetonitrile–water solvent system on a C<SUB>30</SUB> column. The identification of the compounds was based on information from ESI/MS and <SUP>1</SUP>H-NMR.</P><P>Results – NO inhibitory activities for five main fractions of the extract were evaluated, which were identified by LC-NMR-MS as containing furanocoumarins: byakangelicol, oxypeucedanin, imperatorin, phellopterin and isoimperatorin.</P><P>Conclusion – The results obtained showed that the anti-inflammatory activities of A. dahurica could be linked to imperatorin and phellopterin. Copyright © 2010 John Wiley & Sons, Ltd.</P>

Repetitive bending test of membrane electrode assembly for bendable polymer electrolyte membrane fuel cell

Kang, Yun Sik,Park, Taehyun,Jang, Segeun,Choi, Mansoo,Yoo, Sung Jong,Cha, Suk Won Elsevier 2017 Journal of industrial and engineering chemistry Vol.47 No.-

<P><B>Abstract</B></P> <P>Membrane electrode assemblies (MEAs) with carbon paper and cloth for bendable polymer electrolyte membrane fuel cell were characterized as it is subject to repetitive bending. The performance of the MEA with carbon paper was decreased significantly while the MEA with carbon cloth remained constant after repetitive bending. Electrochemical impedance spectroscopy revealed ohmic and charge transfer resistances of the MEA with carbon paper were increased by repetitive bending. Such performance degradation is due to physically observed damages in carbon paper and its detachment from the MEA, which was not in the MEA with carbon cloth due to its intrinsic flexibility.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The MEAs with carbon paper and carbon cloth were characterized as it is subject to repetitive bending. </LI> <LI> The MEA with carbon paper exhibited significant performance degradation after repetitive bending. </LI> <LI> The performance degradation in the MEA with carbon paper resulted from damaged components by cracks and fold lines. </LI> <LI> The MEA with carbon cloth showed stable performance with good bending durability. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

슬관절 손상환자에 있어서 자기공명영상의 진단적 의의

강석,김종필,차응남,김경철,조창성,김용민,황정수,정필현 東國大學校醫學硏究所 1994 東國醫學 Vol.2 No.-

슬관절 손상환자의 진단방법에는 여러 가지가 있으나 자기공명영상은 최근에 발전된, 정형외과영역에서 매우 유용한 진단방법이다. 자기공명영상은 골 및 연부조직의 미세구조를 잘 보여줄뿐 아니라 고도의 정확도를 가진 비 침습적 검사법이다. 본 동국대학교 정형외과학 교실에서는 1994년 1월부터 9월까지 본교실에서 치료받은 슬관절 손상환자에 있어서 이학적 검사, 단순 방사선검사 및 자기공명영상검사후 수술적 방법으로 확진된 30례를 분석하여 비교함으로서 자기공명영상의 진단적 유용성에 대해 알아보고 문헌고찰과 함께 보고하는 바이다. There have been several diagnostic methods to evaluate the knee injury patient with guarded accuracy. Magnetic Resonance Imaging(MRI) is recently developed diagnostic tool of highly improved benefits especially in orthopaedic field. MRI not only shows fine osseus and soft tissue structure, but is not invasive with upgraded accuracy. Authors analysed 30 cases of knee injury of which were diagnosed by simple X-ray including stress view, MRI, and physical examination under anesthesia. All the cases were confirmed at final operative intervention from Jan.1994 to Aug.1994 at our hospital. The purpose of this paper is to appreciate the diagnostic value of MRI in knee injured patient compair with simple X-ray including stress view and physical examination. The sensitivity and specificity of MRI were 78%,100% for ligament injury, and 100%,100% for meniscus injury, respectively. We believe that MRI, when combined with physical examination and X-ray finding gives the most accurate non-invasive method of information to delineate pathologic findings in the knee injury patient.

SF-36을 이용한 근로자들의 건강수준 평가

강명근,박종구,장세진,고상백,차봉석 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.1

This study was conducted to understand health status by general characteristic, and to find out relationship between social support and worker's health status. Health status was measured using SF-36(Medical Outcome Study Short Form 36), a 36 item self administered instrument. The finding of this study were as follow; Mean scores of health status by sex were higher in male. The younger worker reported good health on physical functioning and role limitation-physical than did the older worker, but the older worker reported good health on social functioning and mental health. Mean scores of health status were higher in high income and white worker. When the relationship between social support and health status, social functioning, role limitation-emotion, mental health, vitality, general health were significantly related. Therefore we reviewed the evidence that socio-economic condition are associated with health status in this study, and that the strength of the social support was a important to maintain health.

Removal of parathion by the organophosphorus hydrolase from recombinant Escherichia coli

Suk Soon Choi,Sang Hwan Seo,Dong Gyun Kang,Hyung Joon Cha,Sung Ho Yeom 한국생물공학회 2007 한국생물공학회 학술대회 Vol.2007 No.4

KSHV vPK inhibits Wnt signaling via preventing interactions between β-catenin and TCF4

Cha, Seho,Kang, Myung-Suk,Seo, Taegun Elsevier 2018 Biochemical and biophysical research communication Vol.497 No.1

<P><B>Abstract</B></P> <P>Viral factors interact with host cellular proteins, leading to dysregulation of signaling pathways. The Wnt pathway is known to participate in embryonic development and oncogenesis under dysregulation conditions. A downstream factor of the Wnt signaling pathway, β-catenin, activates T-cell factor (TCF)-dependent transcription, which contributes to cell proliferation and tumorigenesis. In this study, we demonstrated that viral protein kinase (vPK) encoded by Kaposi's sarcoma-associated herpesvirus inhibits the Wnt signaling pathway without affecting nuclear localization and expression of β-catenin. Coimmunoprecipitation and chromatin immunoprecipitation assays revealed that vPK interacts with β-catenin, reducing the binding affinity on TCF binding regions as well as interactions of β-catenin with TCF4. Overexpression of vPK led to reduced mRNA expression of cyclin D1, a well-known transcriptional product of Wnt signaling, suggesting that vPK effectively regulates the host signaling pathway through direct interactions with cellular proteins.</P> <P><B>Highlights</B></P> <P> <UL> <LI> vPK decreases LiCl-mediated activation of TCF-dependent transcription. </LI> <LI> vPK does not disrupt relocalization of β-catenin from the cytosol to nucleus. </LI> <LI> vPK disrupts interactions of β-catenin with the TCF4-binding region. </LI> <LI> vPK inhibits Wnt signaling via interactions with β-catenin. </LI> </UL> </P>

Angiotensin II stimulates the synthesis of vascular endothelial growth factor through the p38 mitogen activated protein kinase pathway in cultured mouse podocytes.

Kang, Young Sun,Park, Yun Gyu,Kim, Bo Kyung,Han, Sang Youb,Jee, Yi Hwa,Han, Kum Hyun,Lee, Mi Hwa,Song, Hye Kyoung,Cha, Dae Ryong,Kang, Shin Wook,Han, Dae Suk Journal of Endocrinology (Ltd. by Guarantee) 2006 Journal of molecular endocrinology Vol.36 No.2

<P>Angiotensin II (Ang-II) and vascular endothelial growth factor (VEGF) have an important role in the pathogenesis of diabetic nephropathy, but the signaling cascade of VEGF regulation in response to Ang-II in podocytes is largely unknown. In these experiments, we looked at the effect of Ang-II on the production of VEGF, and investigated whether VEGF production depends on the p38 mitogen activated protein kinase (MAPK) pathway in cultured mouse podocytes. Incubation of podocytes with Ang-II induced a rapid increase in VEGF mRNA expression and protein synthesis as well as its transcriptional activity in an Ang-II dose-dependent manner. To further define the role of angiotensin type 1 (AT1) and type 2 (AT2) receptors involved in Ang-II-mediated VEGF synthesis, the effects of selective AT1 and AT2 receptor antagonists were evaluated. Prior treatment with losartan significantly inhibited VEGF mRNA and protein synthesis induced by Ang-II, which suggests that the AT1 receptor is involved in Ang-II-mediated VEGF synthesis. Furthermore, stimulation of the cells with Ang-II increased both phosphorylation of p38 MAPK and MAP kinase kinase 3/6 (MKK3/6). Additionally, Ang-II enhanced the DNA binding activity to cAMP response element binding protein (CREB) and phosphorylation of CREB. In addition, to investigate the role of p38 MAPK in Ang-II-induced VEGF synthesis, podocytes were pretreated with or without the p38 MAPK inhibitor, SB203580 for 24 h to observe whether Ang-II-mediated VEGF synthesis was inhibited by blocking p38 MAPK. The addition of SB203580 led to a marked inhibition of the increased VEGF mRNA and protein production induced by Ang-II in a dose-dependent manner. Taken together, these results suggest that Ang-II stimulates the synthesis of VEGF in podocytes and the production of VEGF induced by Ang-II is mediated, in part, through the activation of the p38 MAPK pathway.</P>