Clinicopathological Significance of Elevated PIK3CA Expression in Gastric Cancer

장시형,김경주,오미혜,이지혜,이현주,조현득,한선욱,손명원,이문수 대한위암학회 2016 Journal of gastric cancer Vol.16 No.2

Purpose: PIK3CA is often mutated in a variety of malignancies, including colon, gastric, ovary, breast, and brain tumors. We investigated PIK3CA expression in gastric cancer and explored the relationships between the PIK3CA expression level and clinicopathological features as well as survival of the patients. Materials and Methods: We examined PIK3CA expression in a tissue microarray of 178 gastric adenocarcinomas by immunohistochemistry and reviewed patients’ medical records. Results: In our study, 112 of the 178 gastric cancer patients displayed positive PIK3CA expression. Overexpression of PIK3CA was correlated with low grade differentiation (P=0.001), frequent lymphatic invasion (P=0.032), and high T stage (P=0.040). Patients with positive PIK3CA staining were more likely to display worse overall survival rate than those with negative PIK3CA staining, as determined by Kaplan-Meier survival analysis with log-rank test (P=0.047) and a univariate analysis using the Cox proportional hazard model (hazard ratio=1.832, P=0.051). Conclusions: Elevated PIK3CA expression was significantly correlated with tumor invasiveness, tumor phenotypes, and poor patient survival.

The Morphologic Patterns of Diabetic Nephropathy in Koreans

장시형,박문향 대한병리학회 2009 Journal of Pathology and Translational Medicine Vol.43 No.1

Background : Diabetic nephropathy is the most common cause of end-stage renal disease and it has various pathologic features. We investigated the clinicopathologic differences between the histologic classes of diabetic nephropathy. Methods : A total of 46 patients with diabetic nephropathy were evaluated. Morphologically, the renal lesions were divided into three categories: class 1, diffuse or nodular glomerulosclerosis: class 2, vascular change without evidence of glomerulosclerosis: and class 3, non-diabetic renal disease superimposed on diabetic glomerulosclerosis. We evaluated the laboratory findings and the histologic findings, including mesangial expansion, interstitial fibrosis and inflammation, arteriolar hyalinosis and tubular atrophy. Results : The proportion of each class was 32 cases (70%), 4 cases (9%) and 10 cases (21%), respectively. The clinical and laboratory data showed no significant difference among the classes. For the groups of class 1, the group with nodular sclerosis showed a higher serum creatinine level than did the diffuse group (p=0.003). IgA nephropathy was the most common nondiabetic renal disease superimposed on diabetic glomerulosclerosis in our study. Conclusions : The patients with nodular glomerulosclerosis presented with a more progressed clinicopathological features than did the patients with class 1 diffuse glomerulosclerosis. We also found 21% of all the patients with diabetic nephropathy had superimposed non-diabetic renal disease in a Korean population. Background : Diabetic nephropathy is the most common cause of end-stage renal disease and it has various pathologic features. We investigated the clinicopathologic differences between the histologic classes of diabetic nephropathy. Methods : A total of 46 patients with diabetic nephropathy were evaluated. Morphologically, the renal lesions were divided into three categories: class 1, diffuse or nodular glomerulosclerosis: class 2, vascular change without evidence of glomerulosclerosis: and class 3, non-diabetic renal disease superimposed on diabetic glomerulosclerosis. We evaluated the laboratory findings and the histologic findings, including mesangial expansion, interstitial fibrosis and inflammation, arteriolar hyalinosis and tubular atrophy. Results : The proportion of each class was 32 cases (70%), 4 cases (9%) and 10 cases (21%), respectively. The clinical and laboratory data showed no significant difference among the classes. For the groups of class 1, the group with nodular sclerosis showed a higher serum creatinine level than did the diffuse group (p=0.003). IgA nephropathy was the most common nondiabetic renal disease superimposed on diabetic glomerulosclerosis in our study. Conclusions : The patients with nodular glomerulosclerosis presented with a more progressed clinicopathological features than did the patients with class 1 diffuse glomerulosclerosis. We also found 21% of all the patients with diabetic nephropathy had superimposed non-diabetic renal disease in a Korean population.

High EZH2 Protein Expression Is Associated with Poor Overall Survival in Patients with Luminal A Breast Cancer

장시형,이종은,오미혜,이지혜,조현득,김경주,김성용,한선욱,김한조,배상병,이현주 한국유방암학회 2016 Journal of breast cancer Vol.19 No.1

Purpose: The enhancer of zeste homologue 2 (EZH2) is a catalytic subunit of the polycomb repressive complex 2, a highly conserved histone methyltransferase. EZH2 overexpression has been implicated in various malignancies, including breast cancer, where is associated with poor outcomes. This study aims to clarify nuclear EZH2 expression levels in breast cancers using immunohistochemistry (IHC) and correlate these findings with clinicopathologic variables, including prognostic significance. Methods: IHC was performed on tissue microarrays of 432 invasive ductal carcinoma (IDC) tumors. Associations between EZH2 expression, clinicopathologic characteristics, and molecular subtype were retrospectively analyzed. The relationship between EZH2 protein expression in normal breast tissue and ductal carcinoma in situ (DCIS) was also assessed. Results: High EZH2 expression was demonstrated in 215 of 432 tumors (49.8%). EZH2 was more frequently expressed in DCIS and IDC than in normal breast tissue (p=0.001). High EZH2 expression significantly correlated with high histologic grade (p<0.001), large tumor size (p=0.014), advanced pathologic stage (p=0.006), negative estrogen receptor status (p<0.001), positive human epidermal growth factor receptor 2 (HER2) status (p<0.001), high Ki-67 staining index (p<0.001), positive cytokeratin 5/6 status (p=0.003), positive epidermal growth factor receptor status (p<0.001), and positive p53 status (p<0.001). Based on molecular subtypes, high EZH2 expression was significantly associated with HER2-negative luminal B, HER2-positive luminal B, and HER2 type and triple-negative basal cancers (p<0.001). In patients with luminal A, there was a significant trend toward shorter overall survival for those with tumors having high EZH2 expression compared to those with tumors having low EZH2 expression (p=0.045). Conclusion: EZH2 is frequently upregulated in breast malignancies, and it may play an important role in cancer development and progression. Furthermore, EZH2 may be a prognostic marker, especially in patients with luminal A cancer.

音樂敎育의 成就度에 關한 分析 硏究

張時衡 연세대학교 교육대학원 1977 연세교육과학 Vol.11 No.-

An Unusual Meningothelial Element in a Hairy Polyp of the Hard Palate

장시형,민경환,나웅,장세민,백승삼 대한병리학회 2008 Journal of Pathology and Translational Medicine Vol.42 No.5

Hairy polyps are a rare malformations of bigerminal origin that comprise of both ectodermal and mesodermal elements. Meningothelial elements are an extremely rare pathologic finding in hairy polyps. Here we report a case of a hairy polyp with a meningothelial element, which originated from the hard palate. A 1-year-old boy was evaluated for an intraoral mass accompanied by multiple congenital anomalies. A small polypoid mass was noted at the midline of the hard palate. The lesion had central fibroconnective tissue with an unusual stromal component showing reticulated anastomosing pseudovascular patterns. Immunohistochemical staining of the cells lining the pseudovascular spaces and the interstitial cells revealed vimentin and epithelial membrane antigen positivity. Hairy polyps are a rare malformations of bigerminal origin that comprise of both ectodermal and mesodermal elements. Meningothelial elements are an extremely rare pathologic finding in hairy polyps. Here we report a case of a hairy polyp with a meningothelial element, which originated from the hard palate. A 1-year-old boy was evaluated for an intraoral mass accompanied by multiple congenital anomalies. A small polypoid mass was noted at the midline of the hard palate. The lesion had central fibroconnective tissue with an unusual stromal component showing reticulated anastomosing pseudovascular patterns. Immunohistochemical staining of the cells lining the pseudovascular spaces and the interstitial cells revealed vimentin and epithelial membrane antigen positivity.

GRI, ESRS, ISSB 공시기준 비교연구

장시형,문지호,허성준,이영재 한국무역학회 2023 한국무역학회 학술대회 Vol.2023 No.4

회전익 무인기에 탑재된 다중분광 센서를 이용한 콩의 생체중, 건물중, 엽면적 지수 추정

장시형,류찬석,강예성,전새롬,박준우,송혜영,강경석,강동우,쩌우쿤옌,전태환 한국농림기상학회 2019 한국농림기상학회지 Vol.21 No.4

Soybean is one of the most important crops of which the grains contain high protein content and has been consumed in various forms of food. Soybean plants are generally cultivated on the field and their yield and quality are strongly affected by climate change. Recently, the abnormal climate conditions, including heat wave and heavy rainfall, frequently occurs which would increase the risk of the farm management. The real-time assessment techniques for quality and growth of soybean would reduce the losses of the crop in terms of quantity and quality. The objective of this work was to develop a simple model to estimate the growth of soybean plant using a multispectral sensor mounted on a rotor-wing unmanned aerial vehicle(UAV). The soybean growth model was developed by using simple linear regression analysis with three phenotypic data (fresh weight, dry weight, leaf area index) and two types of vegetation indices (VIs). It was found that the accuracy and precision of LAI model using GNDVI (R2= 0.789, RMSE=0.73 m2/m2, RE=34.91%) was greater than those of the model using NDVI (R2= 0.587, RMSE=1.01 m2/m2, RE=48.98%). The accuracy and precision based on the simple ratio indices were better than those based on the normalized vegetation indices, such as RRVI (R2= 0.760, RMSE=0.78 m2/m2, RE=37.26%) and GRVI (R2= 0.828, RMSE=0.66 m2/m2, RE=31.59%). The outcome of this study could aid the production of soybeans with high and uniform quality when a variable rate fertilization system is introduced to cope with the adverse climate conditions. 콩은 식량작물 중 단백질 함량이 매우 높고 식생활에서 여러가지 형태로 소비되기 때문에 매우 중요한 식량자원 중 하나이다. 콩은 일반적으로 노지에서 재배되기 때문에 콩의 생산량 및 품질은 갑작스런 기후 변화에 큰 영향을 받는다. 최근 폭염 및 폭우 등과 같은 이상기후로 인해 콩의 생산량이 불안정해짐에 따라 콩의 생육을 실시간으로 추정하여 품질저하를 예방할 수 있는 기술 개발이 필요하다. 본 연구에서는 회전익 무인기에 장착된 다중분광 센서를 이용하여 콩 생육을 추정하기 위해 수행되었다. 반사값을 이용하여 산출된 정규화 식생지수(NDVI, GNDVI)와 단순비 식생지수(RRVI, GRVI)와 콩 생육 데이터(생체중, 건물중, 엽면적지수)로 선형회귀분석을 실시하여 생육 추정 모델을 개발하였다. 그 결과, 정규화 식생지수인 NDVI를 이용한 엽면적 지수 추정 모델(R2=0.587, RMSE=1.01 m2/m2, RE=48.98%)보다 GNDVI를 이용한 엽면적 지수 추정 모델(R2=0.789, RMSE=0.73 m2/m2, RE=34.91%)이 높은 정밀도가 나타났으며, 단순비 식생지수를 이용한 엽면적 지수 추정 모델 (RRVI (R2=0.760, RMSE=0.78 m2/m2, RE=37.26%) GRVI (R2=0.828, RMSE=0.66 m2/m2, RE=31.59%)과 비교 했을 때, 단순비 식생지수에서 높은 정밀도가 나타났다. 기후변화에 대체하기 위해 재식밀도 및 변량 시비와 같은 재배관리법이 적용된다면, 고품질의 콩을 생산하는데 도움이 될 것으로 판단된다.

Mediastinal Glomus Tumor: A Case Report and Literature Review

장시형,오미혜,조현득,이지혜,이현주,정혜연,김경주,조성식 대한병리학회 2015 Journal of Pathology and Translational Medicine Vol.49 No.6

A glomus tumor in the mediastinum is very uncommon, and only five cases have been reported in the English literature. We recently encountered a 21-year-old woman with an asymptomatic mediastinal mass that measured 5.3 × 4.0 cm. Surgical excision was performed, and the tumor was finally diagnosed as mediastinal glomus tumor with an uncertain malignant potential. After reviewing this case and previous reports, we analyzed the clinicopathologic features associated with progression of such a tumor.

초분광 영상 기반 머신러닝 알고리즘을 이용한 사과 엽 질소 함량 분류 모델 개발

장시형(Si Hyeong Jang),조정건(Jung Gun Cho),한점화(Jeom Hwa Han),정재훈(Jae Hoon Jeong),이슬기(Seul Ki Lee),이동용(Dongyong Lee),이광식(Kwang Sik Lee) 한국원예학회 2022 한국원예학회 학술발표요지 Vol.2022 No.11

TPC-H를 활용한 MySQL 성능 튜닝

장시형(Si-Hyeong Jang),박기성(Park Gi Sung),이승환(Lee Seung Hwan),신희성(Heeseong Shin),나연묵(Yunmook Nah) 한국정보과학회 2018 한국정보과학회 학술발표논문집 Vol.2018 No.12