위의 장형화생과 동반된 비정형 병소의 H-ras, erb B2, p53 단백의 발현 양상

배한익,김동훈,김정란 대한병리학회 1997 Journal of Pathology and Translational Medicine Vol.31 No.9

c-kit, PDGFRA의 면역조직화학적 발현 양상과 변이 분석을 통한 위장관기질종양 진단의 접근

배한익,김정식,김재훈,오현진,서인수,김종광,강병욱,유완식,정호영 대한병리학회 2010 Journal of Pathology and Translational Medicine Vol.44 No.2

Background : Gastrointestinal stromal tumors (GIST) are the most common mesenchymal tumors in the gastrointestinal tract. Recently, many methods for the diagnosis of GIST have been developed including molecular diagnosis. Methods : We selected 90 cases of GIST that had presented at Kyungpook National University Hospital between 1998 and 2007. Tissue microarrays were made using core areas of tumor tissues. Immunohistochemical staining for c-kit, protein kinase C-theta, and platelet-derived growth factor receptor alpha (PDGFRA) was done. Direct sequencing of hot spot exonal areas for c-kit and PDGFRA were done using extracted DNAs of all 90 paraffin block tissues. Results : Among the 90 cases, 83.3% (75/90) were c-kit positive, 16.6% (15/90) were c-kit negative, 93.3% (84/90) were PDGFRA positive, and 6.6% (6/90) cases were PDGFRA negative. Fifteen cases of c-kit negative GIST included 1 case of PDGFRA negative and 5 cases of PDGFRA negative GIST were ckit positive. The one case in which both c-kit and PDGFRA were negative, showed a c-kit mutation in exon 11. Conclusions : Combined immunohistochemical staining of c-kit, discovered on GIST 1 (DOG1) and PDGFRA is helpful for the diagnosis of GIST. When all staining tests are negative for immunoreactivity, c-kit mutation analysis for exon 11, 9 should be done. Genotyping of kit and PDGFRA do not need to be examined initially, if it is only for the diagnosis of GIST.

주산기 심근병증 1 부검례

배한익,박동일,김정란 東國大學校 1993 東國論叢 Vol.32 No.-

Peripartum cardiomyopathy (PPCM) is a rare form of dilated cardiomyopathy. Onset is from the last trimester of pregnancy to 5 months postpartum. Diagnosis is made by excluding other causes of acute heart failure, such as infections or toxins, and by determining that the patient does not have an underlying cardiac problem that has been unmasked by pregnancy. Its clinical and hemodynamic findings are similar to idiopathic dilated cadiomyopathy : however, peripartum cardiomyopathy shows a high incidience of myocarditis. In addition to congestive heart failure, the occurrence of complications such as sepsis, hepatorenal failure, and arrhythmia were the major causes of death. We experienced an autopsy case of peripartum cardiomyopathy, which is the first in Korea, recently at Pohang hospital of Dongguk University. And we report a pathologic findings of that case with brief review of literatures. The major cause of death in this case is carbon monoxide intoxication with an accelerating association of peripartum cardiomyopathy.

Gastric Mixed Adenoneuroendocrine Carcinoma with Squamous Differentiation: A Case Report

배한익,이채윤,조영민,권오경,유완식,김미선,서안나 대한병리학회 2016 Journal of Pathology and Translational Medicine Vol.50 No.4

위암에서 Epstein-Barr Virus 연관성의 예측 인자

배한익,김영수,남상철,한만훈,정지윤,박선균,서인수 대한병리학회 2008 Journal of Pathology and Translational Medicine Vol.42 No.4

Background : It is important to detect Epstein-Barr virus (EBV) in the setting of gastric cancer so that early viral targeted therapy and prevention can be undertaken. The aim of this study was to investigate the predictive clinicopathologic factors for EBV-related gastric cancer. Methods : The archival tumor tissues from 335 patients with gastric cancer were examined using tissue microarray. The detection of EBV was performed using EBV mRNA in situ hybridization (EBV-ISH), and the results were compared against clinicopathologic factors. Results : EBV-related gastric cancers were identified in 21 of 335 investigated cases (6.27%). The anatomical predisposition of EBV-related cancers to manifest in the upper stomach was statistically significant (p<0.001). EBV-related cancers were almost always (20/21) accompanied by lymphoid stroma. No differences in age, sex, histologic differentiation, or T or N stage were noted between EBV-positive and EBV-negative gastric carcinomas. Conclusions : The association of EBV with gastric adenocarcinomas could be predicted when tumors with lymphoid stroma occurred in the upper stomach.

1998년도 대구지역 법의 부검 통계

배한익,김은석,채종민,조현정,곽정식 大韓法醫學會 1999 대한법의학회지 Vol.23 No.2

This is a report revealing statical cause of death brought to the department of forensic medicine, Kyungpook National University School of Medicine based on the result of autopsy. The summary of result is as follows. 1. The number of autopsy made during 1998 were 356, of these 225 cases(63.2%) were male, and 131(36.8%) were female. 2. 30's, 40's, 20's and 50's was the order according to the number of violent death, and in case of natural death, 30's, 50's, 40's was the oder. 3. August is the period during which autopsies are made most, with December placing second, and the other months are similar. 4. In distributing deaths by cases, violent deaths were 241 cases(67.7%), natural deaths were 105(29.5%) and unknown cases were 10(2.8%). 5. For the violent deaths, deaths due to injury were 128 cases(5301%), asphyxia were 63(26.1%), intoxications were 30(12.4%) and abnormal temperature were 19(7.9%). According to relationship with deaths, homicide is outstanding with 101 cases(41.9%), while suicide and accidental death was the same with 62 cases(25.7%). 6. for death due to injury, death due to blunt injury were 48 cases, sharp force injury which was used most in case of homicide were 43. 7. For the asphyxia, drowning is the most 20 cases, 13 cases of which is accidental death. 8. The deaths of intoxication is the largest part of suicide, and most of intoxication were involved in agrochemical. 9. Of 105 cases of natural death, deaths due to the disease of cardiovascular system were 51 cases(48.6%), of which deaths due to myocardial infraction were 35 cases. 10. Of all autopsy, alcohol intake were checked out in 90 cases and 21 cases of medical controversy took places. 11. Of all violent deaths, the cases involved in the discord between man and woman occupied percentage of 9.6. Of these, homicides were 18 cases, with all sacrificed being females, and of 16 cases of suicide, 13 cases were female.

Genome Analysis Using Array-base Comparative Genomic Hybridization(CGH)

Bae, Han Ik 가톨릭 의과학연구원 2002 가톨릭 의과학연구원 국제학술대회 Vol.6 No.-

Array-based CGH is similar to preexisting chromosomal CGH but uses cytogenetically defined landmarks on the draft sequence of the human genome instead of normal metaphase chromosomes. The landmarks are large-insert clones mapped to chraomsome bands by fluorescence in situ hybridization(FISH). Each clone contains a sequence tag that is positioned on the genomic sequence.

단순 한선 극세포종 3예

신동주,배한익,전재복,이석종,장재원 대한피부과학회 1999 大韓皮膚科學會誌 Vol.37 No.12

Hidroacanthoma simplex (HS) is a rare benign intraepidermal tumor thought to originate from the intraepidermal eccrine duct. Many authors regard it as an intraepidermal form of eccrine poroma but there is still a discussion as to the histogenesis of HS. Clinically, it is more common in older adults and affects both sexes equally. It is found more often on the lower extremities and trunk. The clinical appearance is not characteristic, but the lesions are usually flat or slightly elevated verrucous brown plaques. Histopathologically, the tumor shows characteristic intraepidermal nests and is one of a number of cutaneous lesions showing Borst-Jadasshon phenomenon, that is, tendency to form nests within the epidermis. The tumor cells composing nests show a uniform cuboidal appearance and a round, deeply basophilic nucleus typically. The cytoplasm of tumor cell gives a positive PAS reaction that is diastase-labile. We report herein three cases of HS with the results of immunohistochemical studies.

Endotoxin 投與로 因한 肺胞細胞系의 變化에 關한 超微形態學的 硏究

최동수,배한익,김용진,김중길,곽정식,손태중 慶北大學校 醫科大學 1983 慶北醫大誌 Vol.24 No.2

저자는 endotoxin으로 인한 폐포세포계의 상해기전의 일단을 알아보기 위하여 Sprague-Dawley계 숫놈 흰쥐에 S. typosa O 901 endotoxin을 체중 100gm당 2㎎을 복강내에 주입하고 시간경과에 따라 폐에서 볼 수 있는 형태학적 변화를 광학 및 전자현미경으로 검색하고 또 ruthenium red법에 의한 폐포 피복층의 산성점액 다당류의 동태를 전자현미경적 세포화학적 측면에서 아울러 검색하였다. 그 성적을 요약하면 다음과 같다. 광학 현미경으로는 폐포벽의 모세혈관에서는 혈전 형성, 내피세포의 종창 및 충혈과 간질에서는 부종, 출혈, 세포침윤 및 상피세포의 박탈 등 변화가 관찰되었는데, 이것은 endotoxin 주입 2시간째부터 나타나기 시작하여 시간이 경과함에 따라 점차 심해지는 경향이었다. 무기폐 및 폐기종은 endotoxin 투여후 3시간째부터 나타나기 시작하였다. 전자현미경으로는 30분 내지 1시간부터 모세혈관의 확장과 더불어 모세혈관 내강에는 변성된 백혈구 적혈구 및 혈소판으로 된 미세혈전이 출현하고 1시간째부터는 내피세포에서는 종창, pinocytotic vesicle의 증가, mitochondria의 변성, ER의 종창, lysosome의 증가, 내피세포 간격의 확장 및 기저막 두께의 불규칙과 대식세포 내의 primary lysosome의 감소 및 secondary lysosome의 증가가 일어나고, 2시간째에는 Ⅰ형 및 Ⅱ형 폐포세포에 있어서는 mitochondria의 종창, osmiophilic inclusion body의 공포화 및 숫적 감소, RER의 확장, microvilli의 감소, 폐포강내의 적혈구, 대식세포, Ⅱ형 폐포세포의 출현 등이 관찰되었으며, 폐포 피복층의 산성점액다당류는 endotoxin 투여후 2시간째부터 감소하기 시작하였다. 이상의 변화들은 일단출현하면 시간경과에 따라 더 현저해지는 경향이 있다. 이상의 연구 성저으로 보아 endotoxin 투였시의 폐포 세포계의 상해 기전은 미소순환계에 장해가 먼저 일어나고, 이를 기반으로 하여 Ⅰ형 및 Ⅱ형세포의 변성, 산성점액다당류의 감소, 폐수종 및 무기폐 등 다양한 병리학적 변화가 일어나게 되는 것이라고 생각된다. To investigate the pathogenesis of injury in alveolar wall induced by endotoxin, the author administered 2㎎ of S. typhi O 901 endotoxin per 100 gm of body weight intraperitoneally to male Sprague-Dawley rats. The morphological changes were periodically observed by means of light microscopy, electron microscopy, and cytochemical electron microscopy; ruthenium red stain for identification of acid mucopolysaccharide on alveolar surface. Light microscopically, the changes occurred in alveolar wall were thrombosis, congestion, and swelling of endothelial cells in alveolar capillaries, edema, hemorrhage, and inflammatory infiltration in interstitium, and excoriation of epithelia. These changes began to appear at 2 hours after endotoxin injection and were more pronounced with time. Atelectasis and emphysema began to appear at 3 hours after endotoxin injection. Electron microscopically, at 30 minutes to 1 hour after injection, microthrombi composed of degenerated leukocytes, red cells and platelets, and capillary dilatation appeared. At 1 hour after injertlon endothelial cell showed swelling, increase in number of pinocytotic vesicles, degradation of mitochondria, dilatation of endoplasmic reticulum, increase in number of lysosomes, and widening of intercellular spaces. The basement membrane was irregular in thickness. The macro phages showed decrease in number of primary lysosomes and increase of secondary lysosomes. At 2 hours after injection, type Ⅰ and Ⅱ pneumocytes showed swelling of mitochondria, vacuolization and decrease in number of osmiophilic inclusion bodies, dilatation of RER, and reduction df microvilli. In the alveolar spaces, red cells, leukocytes, macrophages, exocoriated type Ⅱ pneumocytes appeared. Acid mucopolysaccharide on alveolar surface began to reduce in amount at 2 hours after injection. These changes, once appeared, tended to more pronounced with time. At 5 hours after injection of endotoxin, the most of rats in this group were died, the light microscopic changes were severe congestion, hemorrhage, capillary thrombosis, and atelectasis. Electror microscopic changes were appearance of microthrombi composed of degenerated leukocytes, red cells, and platelets, degradation and destruction of endothelial cells, degradation of type Ⅰ and Ⅱ pneumocytes with excoriation degeneration of macrophages, deposition of fibrins, osmiophilic inciusion bodies, red cells, and other degraded cell organelles in alveolar spaces. From the above results, it is conceived that pathogenesis of injury in alveolar wall induced by endotoxin is microcirculatory derangement as the earliest event, and as a result degeneration of type Ⅰ and Ⅱ pneumocytes, decrease of acid mucopolysaccharide, pulmonary edema, atelectasis, and other various pathologic changes could be arose.

만성 위축성 위염및 구강상피 위축을 동반한 악성빈혈 1례

윤형진,배한익,이정호,김성철,전창호,양창헌,이창우 東國大學校醫學硏究所 1994 東國醫學 Vol.2 No.-

악성빈혈은 가장 흔한 비타민 ??의 결핍질환으로 위점막 위축으로 인한 내인자의 분비저하로 발생되며, 근래 환자의 혈청, 타액, 위분비액내의 내인자와 벽세포에 대한 항체를 증명해 냄으로써 위점막의 위축을 자가면역기전으로 설명하려는 경향이 있다. 저자들은 최근 설통 및 흉부 압박감을 주소로 내원한 60세 여자환자가 만성 위축성 위염을 동반한 거적아구성빈혈의 소견을 보이면서 항내인자와 하위벽세포항체가 검출되고, 비타민 ??의 비경구 투여후 급속한 회복을 보여 악성빈혈로 진단하고 이에 문헌고찰과 함께 보고하는 바이다. Pernicious anemia is the most common vitamin ?? deficiency, in which intrinsic factor secretion ceases owing to atrophy of gastric mucosa. On the basis of incomplete evidence, recently pernicious anemia thought to be caused by an autoimmune reaction against gastric parietal cell. A 60 year old female had been admitted to the Department of Internal Medicine, Dong guk University Hospital with chief complaints of sore-tongue and chest pressure. Microscopic examination of peripheral blood and bone marrow disclosed macro-ovalocytic, anisocytic red cell apperance, hypersegmented neutrophils, and hypercellular marrow with megaloblastoid erythropoiesis. Gastroendoscopy with biopsy shows the finding of chronic atrophic gastritis over body to fundus. By using indirect immunofluorescent techniques, we detected the antiparietal cell antibody and antiintrinsic factor antibody. Based on the above findings, a case of pernicious anemia associated with chronic atrophic gastritis could be diagnosed.