객담내 결핵균의 조기발육 시도

김중명,김재식,김경선,이원길,김재룡,전동석,최성만,Kim, Jyung Myung 慶北大學校 醫科大學 1981 慶北醫大誌 Vol.22 No.2

보존 결핵 균주 5주를 가지고 hole slide culture 방법에 의하여 기초 실험을 하고, 이를 바탕으로 하여 객담 108례를 이 방법으로 배양하여 객담내 결핵균의 조기 발육을 시도하여 얻은 성적은 다음과 같이 요약할 수 있었다. 직접 도말 검경에서 양성인 객담15례(14.0%)중 hole slide culture 방법 및 Lowenstein-Jensen 배지에서의 배양 양성은 13례(86.7%)였으며, hole slide culture 방법에서 제 2일차에 이미 심한 균체의 신장이 있었다. 이러한 심한 균체의 신장은 균배양 양성의 가능성을 예견케 하였다. 직접 도말 검경에서 음성인 2례에서 양성 배양이 되었는데 hole slide culture 방법 제 6일차 및 제 8일차에서 심한 균체의 신장이 있었다. The five strains of Mycobacterium tuberculosis were cultured by the hole slide culture method for the basic experiment, and were inoculated on the Loewvenstein-Jensen media simultaneously. The results of the cultures of the microorganisms were all positive in the above method and on the Loewenstein-Jensen media. An early detection of Mycobacterium tuberculosis in 108 case of sputum was attempted by the hole slide culture method as like as the basic experimental test as above, and the results were obtained as follows; In 15 microscopic positive cases on the direct smears, 13 cases of them were positive culture by the hole slide culture method and also on the Loewvenstein-Jensen media, and two cases of them were negative by that method and on that media. In cases of negatives on the smears, two cases were only positive by the method and on the media. The long elongation of bacterial cell was observed on the slide of the second day culture in most of all cases, and there was increses of the number and dimension of the elongated cell on slide of the fourth day culture, and were many groups which composed of the elongated cells of mycobacteria containing granules in the cells on the slide of sixth day culture. In the slides of the eighth day and 10th day culture, the above all figures were mixed each others.

Loewenstein-Jensen 배지(培地)와 Ogawa배지상(培地上)에 있어서의 결핵균(結核菌) 배양(培養) 성적(成績)

이건일 ( Kuhn Il Rhee ),김경숙 ( Kyung Suk Kim ),김중명 ( Jyung Myung Kim ),오성태 ( Sung Tae Oh ),이건희 ( Kun Whi Lee ) 대한임상검사과학회 1980 대한임상검사과학회지(KJCLS) Vol.12 No.1

A total of 706 clinical specimens 546 sputums , 86 cerebrospinal fluids , 32 urines, 23 puses, 15 ascitic fluids and 4 thoracic fluids were cultured into both Lowenstein-Jensen and Ogawa (3%)tb media. Efficiency of two media was compared, and concluded as follow; The positive rate of cultures made into the Lowenstein-Jensen medium was 9.5% and that of Ogawa medium was 4.6%. The length of time required to form colony, the Lowenstein-Jensen medium shorter than those on the Ogawa , and the colonies on the Lowenstein-Jensen medium were more abundant than those on Ogawa medium. Considering the data obtained. it can be stated that the Lowenstein-Jensen medium is superior over Ogawa medium for the primary isolation of Mycobacterium tuberculosis from clinical material that were submitted for the diagnostic purpose.

임상 가검물에서 분리된 균(대장균과 황색 포도균)의 약제 내성

김중명,김재식,김경선,김재룡,전동석,최성만,서상철,김인자,김재숭,이건일,김경숙 慶北大學校 醫科大學 1982 慶北醫大誌 Vol.23 No.2

1981년 1월에서 12월말까지 임상 제료에서 분리한 Escherichia coli (E. coli)와 Staphylococcus aureus (S. aureus) 중에서 무작위로 각 50주 씩 선택하여 원판 확산법과 평판 희섭법으로 그 내성주를 조사하여 다음과 같은 성적을 얻었다. 전체적으로 볼 때에 Teteracyclin (TET), Chloramphenicol (CM) 및 Ampicillin (AMP)에 대한 내성주가 가장 많았고, Amikacin (AMK)에 대한 내성주는 가장 적어다. 이것을 균종별로 보면 E. coli에 있어서는 TET, AMP 및 CM는 원판 확산법으로서 74∼76%, 평판 희석법으로서는 78∼94%가 내성주임을 나타내었고, 또 Cephalothin(CEP)은 원판 확산법에서는 8%가 내성주로 나타났으나, 평판 의석법에서는 52%가 내성주로 나타났다. S. aureus에 있어서는 TET는 원판 확산법으로 82%, 평판 희석법으로서는 78%가 내성주로 나왔고 Penicillin (P)은 원판 확산법에서는 56% 평판 희석법으로는 22%의 내성주가 나왔으며, GM과 CM은 원판확산법에서는 18∼44%, 평판 희석법에서는 56∼58%가 내성주로 나왔다. Each 50 strains of Escherichia coli and Staphylococcus aureus were selected at random out of the stock cultures which were isolated from the clinical materials in the bacteriological laboratory of the department of clinical pathology. Kyungpook National University Hospital from the first of January to the end of December in 1982, and their resistance against eight antibiotics were as follows; Escherichia coli and Staphylococcus aureus had the most resistant pattern against tetracyclin, chloramphenicol, and ampicillin, while they had the most sensitive pattern against amikacin in general. Escherichia coli revealed resistant pattern to tetracyclin, ampicillin, and chloramphenicol 74-76% by the disk diffusion method and 78-94% by the plate dilution method, and revealed resistant pattern to cephalothin 8% by the disk diffusion and 52% by the plate dilution. Staphylococcs aureus disclosed resistant pattern to tetracyclin 82% by the disk diffusion and 78% by the plate dilution, and disclosed resistant pattern to penicillin 56% by the disk diffusion and 22% by the plate dilution, and to gentamicin and-chloramphenicol 18-44% by the disk diffusion and 56-58% by the plate dilution.

T 및 B cell 測定 : 1. 1次 成績 Ⅰ. Results, Primary

金在植,崔成萬,金在龍,全東錫,朴正姬,徐相喆,金仁子,金在崇,金重明 慶北大學校 醫科大學 1982 慶北醫大誌 Vol.23 No.2

E-rosette法에 의한 T cell의 百分率과 螢光抗體法, 感作牛赤血球擔體法, 酵素抗體法 및 마우스赤血球 rosette法에 의한 B cell 의 百分率을 檢査하여 다음과 같이 요약할 수 있었다. 정상성인군의 active T cell은 26±8.2%이었고 total T cell은 72±4.9%이었다. B cell 는 螢光抗體法으로 22±5.4%, 感作牛赤血球擔體法으로 15.8±4.9%, 酵素抗體法으로 15±5.2% 그리고 mouse RBC-rosette法으로 17.2±4.1%이었다. 惡性腫瘍에 있어서 active T cell은 27.±10.4%이었고 total T cell 은 59±11.2%로서 정상인군에 비하여 유의한 감소를 보였다. 良性疾患에 있어서 T cell 은 정상인군에 비하여 유의한 차이를 볼 수 없었다. T cell percent by E-rosette and B cell by fluorescent antibody, senitized ox RBC rosette, enzyme antibody and mouse RBC rosette tests were performed. In healthy adult group the active T cell was 26±8.2% and total T cell was 72±4.9%, B cell by fluorescent antibody was 22.±5.40%, by ox RBC rosette 15.8±4.9%, by enzyme antibody 15±5.2% and by RBC-rosette 17.2±4.1%. In malignant tumor the active T cell was 27±10.4% and total T cell was 59±11.2%. In benign diseases T cell count showed no significant difference from normal adult group.

病的資料에서의 病原菌의 分離

金重明,楊聖基,禹成九,金在植,李健一,金慶淑 慶北大學校 醫科大學 1977 慶北醫大誌 Vol.18 No.2

1976年 9月 1日에서 1977年 8月 31日까지 滿 1年間 血液外 29種目의 病的資料 2846例中 Micro-coccus外 25種目의 細菌 1763株를 分離하여 그 中 1757株를 同定하였는데 그 結果는 다음과 같다. 血液에서는 Staphylococcus epidermidis (Step.ep.) 41株(36.6%), Staphyloccus aureus (Stap.au.) 7株(6.3%), Alpha Streptococcus (Alpra Str.) 6株(5.4%), Streptococcus pneumoniae(St-r.pneu.) 2株(1.8%), Neisseria meningitidis(N.meningi.) 1株(0.9%), Salmonella group A(Sal.GG.A) 8株(7.1%), Salmonella group D (Sal.G.D.) 13株(11.6%), Escherichia coli (Esch.coli)17株(15.2%), Enterobacter aerogenes (Ente.aero.) 4株(3.5%), Enterobactes cloacae (Ente.cloa.) 2株(1.8%), Serratia marcescens (Serr.marc.) 4株(3.5%), Proteus mirabilis (Prot.mira.) 2株(1.8%), Pseudomonas aeruginosa(Ps-eu. aeru.) 3株(2.7%) 및 未同定 2株(1.8%), 都合 112株를 分離하였다. 裵髓液에서는 總 28株를 分離하였는데, 卽 Stap.ep. 7株(25.0%), Stap. au. 1株(3.6%), Apha Str. 8株(28.6%), Str. pneu. 6株(21.4%), N.me-nigi. 1株(3.6%), Esch.coli 3株(10.8) 및 Proteusvulgaris(Prot. vulg.)와 未同定菌 各 一株(3.6%)였다. 心??液에서는 3株가 分離되었는데 Stap.ep.,Alpha Str. 및 Serr. marc.의 各 1株였고, 肋膜液에서는 23株가 分離되었는데, Stap. ep. 5株(21. 7%), Stap. au.와 Ente. aero. 各 3株(13.0%), Alpha Str. 6株(26.1%), Salmonella arizonae(Sal. ariz.)와 Esch. coli 各 2株(8.7%), Prot.vulg.와 Pseu.aeru. 各 1株(4.3%)였다. 胸廓液에서는 Srap. ep. 3株(50.0%), Srap.au. 2株(33.3%), Prot. vulg. 1株(16.7%), 都合 6株가 分離되었고, 腹水 腹膜液 및 腹腔液에서는 Stap ep. 와 Alpha Str. 各 2株(13.3%), Str. pneu. 3株(20.0%), Esxh. coli 8株(53.3%), 都合 15株가, 關節囊液, 穿刺液 및 囊腫液에서는 Stap. au. 2株(50.0%), Alpha Str.와 Prot. mira. 各 1株(25.0%), 都合 4株가 分離되었다. 尿에서는 Microco., Sal.ariz., Klebsiella pneumoniae (Kleb. pneu.),Ente. cloa., Serr. marc. 및 Serratia liquefa-ciens (Sess. liqu.) 各 1株(0.4%), Stap. ep. 80株(32.9%), Stap. au. 17株(7.0%), Alpha Str.30株(12.3%), Esch. coli 66株(27.2%), Ente. aero. 21株(8.6%), Hafnia 2株(0.8%), Prot.vulg. 13株(5.3%), Pseu. aeru. 8株(3.3%), 都合 243株가, 咽喉에서는 Microco. 62株(24.1%), Stap ep. 28株(10.9%), Stap. au. 13株(5.1%),Alpha Str. 49株(19.1%), Beta Streptococcus(Beta Str.), Str. pneu., Hafnia 및 Serr. ma-rc. 各 1株(0.4%), Esch. coli 33株(12.8%), Ente, aero. 1株(8.2%), Prot. vulg. 3株(1.2%),Conynebacterium diphtheriae (Cory. diph.) 36株(14.0%), Candida albicans (Cand. albi.) 8株(3.1%), 都合 257株가 分離되었다. 鼻에서는 Mi-croco.와 Prot. mira. 各 2株(2.8%), Stap. ep.26株(36.6%), Stap. au. 14株(19.7%), Alpha Str. 5株(7.0%), Esch. coli 19株(26.8%), Ente. aero. 3株(4.2%), 都合 71株가, 喀痰에서는 Micro co. 201株(39.8%), Stap. ep. 25株(5.0%), Stap.au. 9株(1.8%), Alpha Str. 115株(22.8%), Str.pneu. 22株(4.4%), Sal. ariz., Kleb.pneu. 및 Serr. marc. 各 3株(0.6%), Esch. coli 50株(9.9%), Ente.aerc.와 Prot. vulg. 各 13株(2.6%), Ente. cloa., Hafnia 및 Serr. liqu. 各 1株(0.2%), Pseu. aeru. 8株(1.6%), Cand. albi. 37株(7.3%), 都合 505株가, ??에서는 Stap. ep., Stap.au., Sal.G.A., Sal.G.B., Sal.G.C. 및 Serr. liqu. 各 1株(2.1%), Alpha Str. 4株(8.5%), Esch. coli 30株(63.8%), Ente. acro. 2株(4.3%), Prot. mira. 5株(10.6%), 都合 47株가 分離되었다. 膿에서는 Microco.와 Kleb. pneu. 各 2株(0.7%), Stap. ep. 41株(15.2%), Stap. au. 55株(20.4%), Alpha Str. 28株(10.4%), Beta Str.4株(1.5%), Str. pneu., Sal. ariz. 및 Ente. cloa. 各 1株(0.4%), Esch. coli 50株(18.6%), Ente. aero.와 Pseu. aeru. 各 23株(8.6%), Haf-nia 5株(1.9%), Serr. marc. 10株(3.7%), Pr-ot. vulg. 20株(7.4%), Colstridium welchii(Cl-os. welch.) 3株(1.1%), 都合 269株가 傷處排出物에서는 Microco. 와 Hafnia 各 1株(1.9%), Stap. ep. 6株(11.1%), Stap. au.와 Ente. aero. 各 2株(5.6%), Alpha Str. 8株(14.8%), Esch. coli 13株(24.1%), Prot. vulg. 9株(16.7%), Pseu. aeru. 10株(18.5%), 都合 54株가 分離되었으며, 組織片에서는 Stap. ep. 7株(38.9%), Stap. au., Esch. coli 및 , Ente. aero. 各 3株(16.7%), Alpha Str. 2株(11.1%), 都合 18株가, 直腸塗抹物에서는 Stap. ep.와 Alpha Str. 各 7株(10.3%), Esch. coli 34株(50.0%), Ente. aero. 5株(7.4%), Serr. marc. 1株(1.5%), Prot. vulg. 4株(5.9%), Prot. mira. 10株(14.7%), 都合 68株가, 臍帶에서는 Stap. ep. 15株(37.5%), Stap. au.와 Alpha Str. 各 3株(7.5%), Esch. coli 11株(27.5%), Kleb.pneu., Prot. vulg. 및 Prot. mira. 各 1株(2.5%), Ente. aero. 2株(5.0%) 및 未同靜菌 3株(7.5%), 都合40株가 分離되었다. 2種細菌이 同時에 分離된 것이 37例였는데 이 中에서 Microco. 와 Alpha Str.의 混合이 가장 많았고, 3種細菌이 同時에 分離된 것은 8例가 있었는데, M-icroco.와 Alpha Str. 와 Esch. coli의 混合이 가장 많았다. During one year, from 1st of September 1976 to 31st of August 1977, 1757 strains of microorganisms that were consisted of two genera, four groups of Salmonella and 20 species of microorganisms were isolated form cultures of the 2846 cases of the pathogenic specimens that were contained 30 kinds of the clincial materials which were the blood, C.S.F., pericardial fluid, pleural fluid, thoracic fluid, ascitic, peritoneal and abdominal fluids, synovial, aspirated and cystic fluids, urine, throat specimens, nasal specimens, sputum, stool, pus, wound discharges, tissue specimens, rectal swabs and umbilical cords. The results were as follows; 41 strains (86.6%) of Staphylococcus epidermidis (Stap. ep.), 7 strains (6.3%) of Staphylococcus aureus (Stap. au.), 6 strains (5.4%) of alpha Streptococcus (Alpha Str.)., 2 strains (1.8%) of Streptococcus pneumoniae (Str. pneu.), 1 strain (0.9%) of Neisseria meningitidis (N. meningi.)., 8 strains (7.1%) of Salmonella group A (Sal. G.A.), 13 strains (11.6%) of Samonella groupd D (Sal. G.D.), 17 strains (15.2%) of Escherichia coli (Esch. coli), 4 strains (3.5%) of Enterobacter aerogenes (Ente. aero.), 2 strains (1.8%) of Enterobacter cloacase (Ente. cloa.), 4 strains (3.5%) of Serratia marcescens(Serr. marc.). 2 strains (1.8%) of Proteus mirabilis(prot. mira.), 3 strains(2.7%) of Pseudomonas aeruginosa (Pseu. aeru.) and 2 strains (1.8%) of undetermined organisms (Undetermin.), total 112 strains, were siolasted from 845 cases of the blood cultures. In the C.S.F. 28 strains were isolated from 239 cases of the cultures, they were 7 strains (2.5%) of Stap. ep., 1 strain (3.6%) of Stap. au., 8 strains (28.6%) of Alpha Str., 6 strains(21.4%) of Str. pneu., 1 strain (3.6%) of N. meningi., 3 strains (10.7%) of Esch. coli, 1 strain(3.6%) of Proteus vulgaris( Prot. vulg.) and Undetermin. In the pleural fluid, 23 strains were isolated from 85 case of the cultures, they were 5 Stap. ep. (21.7%), 3 Stap. au. and Pseu.Prot.(13.0%), 6 Alpha Str.(26.1%) 2 Salmonella arizonae(Sal. ariz.)and Esch. coli (8.7%), 1 Prot. vulg. and Pseu. aeru. ( 4.3%). In the thoracic fluid, 6 strains of 17 cases were isolated, they were 3 Stap. ep. (50.0%) 2 Stap. au. (33.3%) and 1 Prot. vulg. (16.7%). 15 strains of 34 cases were isolated form the ascitc, peritoneal and abdominal fluids, they were 2 Stap. au. and Alpha Str. (13.3%), 3 Str. pneu. (20.0%), 8 Esch. coli (53.3%). 4 strains of 17 cases were isolated from the synovial, aspirated and abdominal fluids, they were 2 Stap. au. (50.0%), 1 Alpha Str. and Prot. mira (25.0%). In the urine, 243 strains were isolated from 280 cases, they were 1 Micrococcus (Microco.), Sal. ariz., Klebsiella pneumoniae (Kleb. pneu.), Ente. cloa., Serr. marc. and Serratia liquefaciens (Serr. liqu.) (0.4%), 80 Stap. 데. (32.9%), 17 Stap. au. (7.0%), 30 Alpha Str. (12.3%), 66 Esch, coli (27.2%), 21 Ente. aero. (8.6%), 2 Hafnia (0.8%), 13 Prot. vulg. (5.3%) and 8 Pseu. aeru. (3.3%). 2 Microco. and Prot. mira. (2.8%), 25 Stap. au. (36.6%), 14 Stap. au.(19.7%), 5 Alpha Str. (7.0%), 19 Esch coli (26.8%) and 3 Ente. cloa. (4.2%), total 71 strains were isolated form 64 cases of the nasal specimen cultures. In the Sputum, 505 strains were isolated form 298 cases of cultures, they were 201 Micrco. (39.8%), 25 Stap. ep. (5.0%), 9 Stap. au. (1.8%), 115 Alpha Str. (22.8%), 22 Str. pneu. (4.4%), 3 Sal. ariz., Kleb pneu. and Serr. marc. (0.6%), 50 Esch. coli (9.9%), 13 Ente. aero. and Prot. vulg. (2.6%), 1 Ente. cloa., Hafnia and Serr. liqu.(0.2%), 8 Pesu. aeru. (1.6%) and 37 Cand. albi. (7.3%). In the stool, 57 strains were isolated from 181 cases of the cultures, they were 1 Stap. ep., Stap. aul, Sal. G.A., Sal. G.B., Sal. G.C. and Serr liqu.(2.1%), 4 AlphaStr. (8.5%), 30 Esch coli (63.8%), 2 Ente. aero. (4.3%) and 5 Prot. mira. (10.6%). Int he pus, 269 strains were isolated form 300 cases of the cultures, they were 2 Microco. and Kleb. pneu. (0.7%), 41 Stap. ep. (15.2%), 55 Stap. au. (20.4%), 28 Alpha Str. (10.4%), 4 Beta Str. (1.5%), 1 Str. penu., Sal. ariz. and Ente. cloa. (0.4%), 50 Esch. coli (18.6%), 5 Hafnia (1.9%), 10 Serr. marc. (3.7%), 20 Prot. vulg. (7.4%) and 3 clostridium welchii (Clos. welch.) (1.1%). In the wound discharge, 52 strains were isolated form 54 cases of the cultures, they were 1 MIcroco. and Hafnia (1.9%), 6 Stap. ep. (11.1%), 3 Stap. au. and Ente. aero. (5.6%), 8 Aphla Str. (14.8%), 13 Esch. coli (24.1%), 9 Prot. vulg. (16.7%) and 10 Pseu. aeru. (18.5%). In tip and tissue specimesna, 18 strains were isolated from 21 cases of the cultures, they were 7 Stap. ep. (38.9%), 3 Stap. au. Esch. coli and Ente. aero. (16.7%), and 2 Alpha Str. (11.1%). In rectal swabs, 68 strains were isolated from 60 cases of the cultures, they were 7 Stap. ep. and Aphla Str. (10.3%), 34 Esch. coli (50.0%), 5 Ente. aero. (7.4%), 1 Serr. marc. (1.5%), 4 Prot. vulg. (5.9%), and 10 Prot. mira. (14.7%). In the umbilical cord, 40 strains were isolated form 34 cases of the clutres, the were 15 Stap. ep. (37.5%), 3 Stap. au. and Aphla Str. (7.5%), 11 Esch. coli (27.5%), 1 Kleb. pneu. Prot. vulg. and Prot. mira. (2.5%), 2 Ente. aero. (5.0%) and 3 Undetermin. (7.5%). There were 37 cases that two kinds of microorganisms were isolated at the same time and synchronous isolation of the Alpha Str. and Microco. was the most, and were 8 cases that three kinds of microorgainsms were simultaneously isolated and synchronous isolation of Micrco., Alpha Str. and Esch. coli was the most.

各種 植物의 抗菌作用에 관한 硏究(第二報) : 一般植物의 스크리닝檢査에 依한 抗菌作用에 關한 硏究 Screening Test for Determining Antibiotic Activity of Various Plants

金重明,金在植,金鍾律 慶北大學校 醫科大學 1975 慶北醫大雜誌 Vol.16 No.1

黃色葡萄球菌의 抗生劑에 대한 感受性

金重明,金在植,全孝鎭,朴銀美,徐長洙,金慶淑 慶北大學校 醫科大學 1985 慶北醫大誌 Vol.26 No.4

경북대 의대 부속병원 임상병리과에서 1981년 1월부터 1985년 12월까지 임상재료로부터 분리된 S. aureus 179주를 대상으로 8종의 항생제에 대해 감수성 시험을 한천희석법으로 실시하여 다음과 같은 결론을 얻었다. 1. MIC 50은 Pc-G 및 Cep가 각각 0.25∼1㎍/㎖로 가장 낮았으며, Tc가 32∼64㎍/㎖로 가장 높았다. MIC 90은 Cep가 8∼16㎍/㎖로 가장 낮았고 Gm이 7∼56㎍/㎖로 가장 높았다. 2. 항생제 내성율은 Pc-G에 대해 77∼84%로 가장 높았고, Cep에 대한 것이 4∼80%로 가장 낮았으며 연차적 내성균의 증감은 4∼7%였다. 3. 1985년에 분리된 균주 중 MRSA는 17%였고, Tc, Pc-G, Met, C-Tm, Cfz에 대해 동시내성을 가지는 균주가 2주, Tc, Pc-G, Met, Cfz 및 Tc, Pc-G, C-Tm에 동시내성을 가지는 균주가 각각 3주 있었다. 179 strains of S. aureus were selected at random out of stock cultures which were isolated from the clinical specimens in the clinical laboratory from Jan. 1981 to Dec. 1985, Kyungpook National University Hospital, and their sensitvrity pattern for 8-antimicrobial agents were analyted by the agar dilntion method. For the lowest level of MIC 50 and MIC 90 of antimicrobial susceptibilities, MIC 50 of penicillin G(Pc-G) and cephalothin (Cep) were 0.25∼1㎍/㎖, and MIC 90 of Cep is 8∼16㎍/㎖. For the highest level of MIC 50 and MIC 90, MIC 50 of tetracyclin (Tc) was 32∼64㎍/㎖, and MIC 90 of Gm was above 256㎍/㎖ For PcG, S.aureus showed the highest resistance as 77∼84%, and for Cep, showed the lowest resistance as 4∼7%. Comparing the percentage of resistance from 1985 to 1981 to Cep and Tc showed increased resistance as 4% and 5% respectively. In 1985 methicillin resistnat S. aureus was seen in 17% of strains isolated and two strains were a multiuple drug resistant to all antimicrobial agent applied, three strains were resistant to were a multiple drug resistant to all antimicrobial agent applied, three strains were resistant to Tc, Pc-G, methicillin and Cep, and three strains were resistantto Tc, Pc-G and cotrimoxazole.

임상재료에서 분리된 대장균의 항생제 감수성

김중명,김재식,전효진,박은미,서장수,김경숙 慶北大學校 醫科大學 1984 慶北醫大誌 Vol.25 No.3

경북대학교 의과대학 부속병원 임상병리과에서 1981년 1월부터 1984년 12월까지 임상재료로부터 분리된 E. coli 555주(1981년에 50주, 1982년에 105주, 1983년에 97주 및 1984년에 303주)를 임의로 선택하여 disk diffusion method로, 이 중 각 년도별로 다시 50주를 임의로 선택하여 plate dilution method로 Ap, Ce, Cm, Tc, Ak, Gm 및 Tb에 대한 감수성과 그의 연차적 추이를 보았고, 그 내성균주에 있어서는 검체별 분포, 연차적증감 및 다체내성과 그 검체별 분포를 보아 다음과 같이 요약할 수 있었다. Ap, Ce, Cm, Tc, Ak, Gm 및 Tb에 대한 감수성에 있어서는 disk diffusion method의 경우에는 Ak에서 가장 높았고 Tc에 가장 낮았으며, 년차적 증감은 큰 변화가 없었으며 plate dilution method의 경우에는 Ak에서 가장 높았고 dilution method의 경우에는 Ak에서 가장 높았고 Ap에 가장 낮았으며 년차적 증강은 대체로 감소하는 추세였다. 내성균주의 재료별 분포를 보면 뇨, 농 및 객담 및 기관지분비물에서 높았으며 그 출현율도 그 재료에서 높았으며 다제내성은 1제에서 7제에서 걸쳐있었는데, 7제내성균주가 16주 있었으며, 특히 소변 유례의 7제내성균주는 9주나 있었다. The bacteria tested were 555 strains of Escherichia coli(E. coli, 1981-50 strains, 1982-105 strains, 1983-97 strains and 1984-303 strains), and were isolated in our clinical laboratory from Jan. 1981 to Dec. 1984. The antimicrobial susceptibility agains 7 antimicrobial agents, which composed of ampicillin, cephalothin, chloramphenicol, tetracycline, amikacin, gentamicin and tobramycin, were perfomed by means of a single high potency disk diffusion test and a plate dilution test. E. coli showed the highest sensitivity to amikacin as 93-96% by disk diffusion method and 70-92% by plate dilution method, and the lowest sensitivity to tetracycline as 18-23% by disk diffusion method and to ampicillin as 8-24% by plate dilution method, and relatively high sensitivity to gentamicin and tobramycin and relatively low sensitivity to chloramphenicol by both methods. The susceptible strains decreased by year which were tested by plate dilution method but not incresed or decresed by disk diffusion method. The resistant strains of E. coli were distributed mainly in blood, urine, pus, sputum and bronchial secrete, and the rates of appearance were all high in above specimens, and multiple drugs resistance of E. coli were related up to seven drugs, and there were 16 strains which were resistant agains 7 agents above mentioned and especialy high distributed in urine as 9 strains.

病的資料에서의 病原菌의 分離

金重明,楊聖基,禹成九,金在植,李健一,金慶淑 慶北大學校 醫科大學 1977 慶北醫大雜誌 Vol.18 No.2

各種 植物의 抗菌作用에 關한 硏究(第二報) : 一般植物의 스크리닝檢査에 依한 抗菌作用에 關한 硏究 Report II: Screening Test for Determining Antibiotic Activity of Various Plants

金重明,金在植,金鍾律 慶北大學校 醫科大學 1975 慶北醫大誌 Vol.16 No.1

山野에 흔히 있는 120種의 植物을 5月~11月까지 任意로 採取하여 얻은 잎, 꽃, 줄기, 뿌리 및 열매 等 309例의 材料를 가지고 Petroleum ether, (P.ether) 蒸溜水(W.dist) 1%HCl液 및 10%Na_2CO_3液의 4種이 溶媒로 各各 所定의 方法에 依해서 溶出處理해서 얻은 推出液 1236例를 가지고 Staphylococcus aureus 209P(S.aureus)와 Escherichia Coli O111(E.coli)을 對象으로 disk 方法에 依한 Screening test를 하여 다음과 같은 要約을 얻을수 있었다. 1. 全般的으로 1236例中 837例(67.7%)에서 抗菌作用을 볼 수 있었는데 卽 S.aureus에만 있는 것이 96例(7.8%), E.coli에만 있는것이 157例(12.7%), S.aureus와 E.coli의 兩者에 함께 있는것이 584例(47.2%)이었다. 2. 溶出 處理한 推出液別로 그 抗菌作用을 나타낸 것을 보면 P.ether 推出液의 96例(31.0%), n=309), W.dist 推出液이 107例(34.6%, n=309), 1%HCl液 推出液이 181例(58.6%, n=309), 10%Na_2CO_3液 推出液이 453例(146.7%, n=309)었는데 이것을 供試菌別로 보면 P.ether 推出液에서 S.aureus에만 作用한것이 33例(10.7%, n=309), E.coli에만 作用한것이 19例(6.1%, n=309), 兩供試菌에 같이 作用하는 것이 44例(14.2%, n=309)이었고 W.dist.推出液에 S.aureus에만 作用한것이 28例(9.1%, n=309), E.coli에만 作用한것이 19例(6.1%, n=6.1%), 兩菌에 같이 作用한 것이 60例(19.4%, n=309), 1%HCl 推出液에서 S.aureus에만 作用한것이 23例(7.4%, n=309), E.coli 만 作用한것이 40例(13.0%, n=309), 兩菌에 같이 作用한 것이 118例(38.2%, n=309), 10%Na_2CO_3 推出液에서 S.aureus에만 作用한것이 12例(3.9%, n=309), E.coli에만 作用한것이 79例(25.6%, n=309), 兩菌에 같이 作用한 것이 362例(117%, n=309)이었다. 3. 5~11月까지 月別로 나타난 抗菌作用을 보면 S.aureus에서는 5月에 144例中 58例(40.2%), 6月에 284例中 122例(43.0%), 7月에 272例中 119例(43.8%), 8月에 172例中 40例(23.2%), 9月에 172例中 25例(14.5%), 10月에 60例中 15例(25.1%), 11月에 132例中 9例(6.9%)었고, E.coli에서는 5月에 144例中 50例(34.8%), 6月에 284例中 121例(42.7%), 7月에 272例中 135例(49.7%), 8月에 172例中 54例(31.4%), 9月에 172例中 48例(27.9%), 10月에 60例中 17例(28.4%), 11月에 132例中 24例(18.2%)로서 兩供試菌에 있어서 모두 5,6 및 7月에 採取한 植物材料에 抗菌作用이 많이 나왔으며 同時에 月別變動도 볼 수 있었다. 4. 120種의 植物에서 얻은 309例의 植物材料中에서 가장 强한 抗菌作用을 나타낸 것은 S.aureus에 對해서는 Commelins communis L의 잎을 W.dist.로 溶出한 推出液이었는데 ??었고, E.coli에 對해서는 Pinus densiflora Sieb. et Zucc.의 잎과 樹皮를 10%Na_2CO_3 液으로 溶出한 推出液이었는데 ??었다. 5. 以上의 結實로서 一般植物을 資源으로한 抗菌作用物質이 存在할 可能性이 있다고 본다. (實驗을 實行하는데 本敎室 金慶淑孃의 誠心이 가득찬 協助에 크게 힘입어 여기 謝意로 表한다) It is the purpose this report to observe the potential antibiotic activities of the various plants which are chiefly growing and populary finding on the fields and mountains in Korea. The plants for the samples which are 309plant specimens that are conrained the leave, flowers, stems, roots, fruits and seeds etc. or whole grasses from the 120 kinds of plant from May to November 1974. These plant specimenes were extracted with the petroleum ether, water distilled, 1%HCL and 10% Na_2CO_3 solution, and the authers obtained 1236 extracts by the method. The extracts of 1236 were screened for the presence of thier potential antibiotic activities against the Staphylococcus aureus 209 P(S. aureus) and Escherichia coli O111(E.coli) and the results are as follows: 1. Generally the 837 extracts (67.7%) have the potential antibiotic effects against the S.aureus and E.coli, 96 extracts (7.8%) have the antibiotic effects against the S.aureus only and 157 extraets (12.7%) have the effects against the E.coli only and 584 extracts (47.2%) have the effects against the both microorganisms tested. 2. The antibiotic effects of the plant extracts by the solvents against the S.aureus and E.coli are as follows: 96 extracts (31.0%, n=309) have the effects in the solvent of the petroleum ether, 107 extracts (34.6%, n=309) in the water distilled, 181 extracts (58.6%, n=309) in the 1%HCL solution and 453 extracts (146.7%, n=309) in the 10%Na_2CO_3 solution. The effects of the plant extracts against the both microorganisms are as follows: 33 extracts (10.7%, n=309) have the effects against S.aureus, 19 extracts (6.1%, n=309) against the E.coli, and 44 extracts (14.2%, n=309) have the effects against S.aureus, 19 extracts (6.1%, n=309) against E.coli, and 60 extracts (19.4%, n=309) against the both in water distilled extraction 23 extracts (7.4%, n=309) against the S.aureus, 40 extracts (13.0%, n=309) against the E.coli, and 118 extracts (38.2%, n=309) against the both in the 1%HCL extraction. 12 extracts (3.9%, n=309) against the S. aureus, 79 extracts (25.6%, n=309) against E.coli, and 362 extracts (117.2%, n=309) have the effects against the both in the 10%Na_2CO_3 extraction. 3. The antibiotic effects of the plant specimens which were collected for the samples from May to November, 1974 are as follows: Against the S.aureus, 58 (40.2%) of 144 plant extracts in May, 122 (43.0%) of 284 plant extracts in June, 119 (43.8%) of 272 plant extracts in July, 40(23.2%) of 172 plant extracts in August, 25(14.5%) of 172 plant extracts in September, 15(25.1%) of 60 plant extracts in October and 9 (6.9%) of 132 plant extracts in November have the effects. Against the E.coli, 50(34.8%) of 144 plant extracts in May, 121 (42.7%) of 244 plant extracts in June, 135 (49.7%) of 272 plant extracts in July, 54(31.4%) of 172 plant extracts in August, 42 (27.9%) of 172 plant extracts in September, 17 (28.4%) of 60 plant extracts in October and 24 (18.2%) of 132 plant extracts in November have the effects. The plant specimens obtained in May, June and July have more the antibiotic effects than the other specimens collected in August, September, October and November. The antibiotic effects of the plants are changeable by the month or season. 4. The leave of the Commelins communis L.extracted with water distilled have the strogest antibiotic effects (??) against the S.aureus, and the leave and cutis of the Pinus densiflora Sieb. et Zucc. extracted with 10%Na_2CO_3 solution have the strongest effects (??) against the E.coli in the all of plant specieses, 120 kinds tested. 5. The existence of the antibiotic effective substances in the various plants may be possioble to foretold by the above results.