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        Loewenstein-Jensen 배지(培地)와 Ogawa배지상(培地上)에 있어서의 결핵균(結核菌) 배양(培養) 성적(成績)

        이건일 ( Kuhn Il Rhee ),김경숙 ( Kyung Suk Kim ),김중명 ( Jyung Myung Kim ),오성태 ( Sung Tae Oh ),이건희 ( Kun Whi Lee ) 대한임상검사과학회 1980 대한임상검사과학회지(KJCLS) Vol.12 No.1

        A total of 706 clinical specimens 546 sputums , 86 cerebrospinal fluids , 32 urines, 23 puses, 15 ascitic fluids and 4 thoracic fluids were cultured into both Lowenstein-Jensen and Ogawa (3%)tb media. Efficiency of two media was compared, and concluded as follow; The positive rate of cultures made into the Lowenstein-Jensen medium was 9.5% and that of Ogawa medium was 4.6%. The length of time required to form colony, the Lowenstein-Jensen medium shorter than those on the Ogawa , and the colonies on the Lowenstein-Jensen medium were more abundant than those on Ogawa medium. Considering the data obtained. it can be stated that the Lowenstein-Jensen medium is superior over Ogawa medium for the primary isolation of Mycobacterium tuberculosis from clinical material that were submitted for the diagnostic purpose.

      • 血液, 脊髓液, 肋膜腔液, 腹腔液 및 膿에서 分離된 病原菌

        金重明,金在植,李健一,金慶淑,金京善,李源吉,金在龍 慶北大學校 醫科大學 1979 慶北醫大誌 Vol.20 No.2

        病的 資料로서 依賴받은 血液(924例), 脊髓液(203例), 肋膜腔液(36例), 腹腔液(103例) 및 膿(297例)으로된 都合 1563例를 對象으로 하여 1978年 3月 初부터 1979年 2月 末까지 一年間 分離 培養, 感受性 檢査 等을 하여 그 成績을 다음과 같이 要約할 수 있었다. 全體的인 分離 培養 陽性率은 29.0%(454/1563) 資料別로는 血液이 17.4%(161.\/924), 腦脊髓液이 5.9%(12/203), 肋膜腔液이 22.3%(23/103), 腹腔液이 27.8%(10/36), 膿이 83.5%(248/297)였다. 分離菌은 coagulase 陰性 葡萄球菌 82株(18.1$), coagulase 陽性 葡萄球菌 42株(9.3%), α-連??球菌 28주(6.2%), β-連??球菌 2株(0.4%) 肺炎球菌 3株(0.7%), Serratia 5株(1.1%), 大腸菌 135株(29.7%), Alkaligenes 1株(0.2%), Enterobacter 47株(10.3%), Salmonella 17株(3.8%), Arizona 1株(0.2%), Citrobacter 2株(0.4%), Klebsiella 14株(3.1%), Pseudomonas 22株(4.8%), Protesu 45株(9.9%), 枯草菌 2株((0.4%), Clostridium 6株(1.3%)였다. 資料別로 血液에서 coagulase 陰性 葡萄球菌 35株(21.7%)coagulase 腸性 葡萄球菌 7株(4.4%), α-連??球菌 3株(1.9%), 肺炎球菌 1株(0.6%), Serratia 2株(1.2%), 大腸菌 56株(34.8%), Alkaligenes 1株(0.6%), Enterobacter 27株(16.7%), Salmonella 17株(10.6%), Klesiella 3株(1.9%), Pseudomonas와 Proteus 각 4株(2.5%), clostridium 1株(0.6%)였고, 腦脊髓液에서는 coagulse 陰性 葡萄球菌 5株(41.7%), α-連??球菌, 大腸菌, Pseudomonas 各 1株(8.3%), 肺炎球菌 외 Proteus 各 2株(16.7%)였고, 肋膜腔液에서는 coagulase 陰性 葡萄球菌과 陽性 葡萄球菌 各 2株(8.7%), α-連??球菌, Proteus, 枯草菌 各 1株(4.3%), 大腸菌 9株(39.2%), Enterobacter 4株(17.4%), Pseudomonas 3株(13.1%)였다. 腹腔液에서는 coagulase 陰性 葡萄球菌, coagulase 陽性 葡萄球菌, 枯草菌 各 1株(10.0%) 大腹菌 5株((50.0%), Klebsiella 2株(20.0%)였으며 膿에서는 coagulase 陰性 葡萄球菌 39株(15.7%), coagulase 陽性 葡萄球菌 32株(12.9%), α-連??球菌 23株(9.3%), β-連??球菌과 Citrobacter 各 2株(0.8%), Serratia 3株(1.2%), 大腸菌 64株(25.7%), Enterobacter 16株(6.4%), Arizona 1株(0.4%), Klebsiella 9株(3.6%), Pseudomenas 14株(5.7%), Proteus 38株(15.4%), Clostridium 5株(2.0%)였다. 月別, 季節別 分離 狀況에는 有義的인 特徵은 볼 수 없었고 Gram 陰性 ??菌과 陽性球菌에 있어서 分離 陽性率은 球菌보다 ??菌이 더 높았다. 感受性 檢査 成績은 coagulase 陰性 葡萄球菌은 Novobiocin(NB)에 40.0%, Carabnicillin(CBPC)과 Lincomycin(LCM)에 各 30.0%, Kanamyci(KM), Streptomycin(SM), Amikacin(AK), Doxycycline(DC)에 各 20.0%, Penicillin G (PC-G), Erythromycin(EM), Chloramphenicol(CP), Tetracycline(TC)에 各 10.0%,의 感受性株(S)가 있었고, Neomycin(NM)에 感受性株(S)는 없어TEk. Coagulase 陽性 葡萄球菌은 CBP에 40.0% LCM 30.0%, EM, CP, NB에 各20.0%, NM, AK, DC에 各 10.0%의 S가 있었다. PC-G, KM, SM, TC에는 S가 없었다. α-連??球菌은 LCM에 50.0% DC에 30.0%, EM에 20.0%, PC-G, CBPC, KM, CP, TC, NB, AK에 各 10.0%의 S가 있었고 NM, SM에는 S가 없었다. 大腸菌은 CBPCDP 40.0%, NM, CP에 各 20.0%, KM, TC, NB, AK, DC에 各 10.0%의 S가 있었고, PC-G, SM, EM, LCM에는 S가 없었으며, Enterobacter는 CBPC와 DC에 10.0%의 S가 있었고, PC-G, KM, NM, SM, EM, CP, TC, NB, LCM, AK에는 S가 없었다. Pseudomonas에는 供試 藥劑의 全部에 S는 없었으며, Proteuns는 CBPC에 20.0%, KM, SM, CP, AK에 各 10.0%의 S가 있었고, 그 外에 藥劑에는 S가 없었다. During one year from second March 1978 to 28th February 1979, we had isolated 454 strains (29.0%) of microorganisms out of 1563 specimens which were consisted of 924 cases of blood, 203 cases of cerebrospinal fluid(CSF), 103 cases of thoracic fluid, 36 cases of abdominal fluid, and 297 cases of puses, and the results were follows; The rates of of the positive cultures were 17.4%, 5.9%, 22.3%, 27.8%, and 83.5% in the case of blood, CSF, thoracic fluid, abdominal fluid, and puses repectively. The microorganisms were 82 strains of the coagulase negative Staphylococci (18.1%), 42 strains of the coagulase positive Staphylococci (9.3%) 23 strains of the alpha Streptococci (6.2%), two strains of the beta Streptococci (0.4%), three strains of the Streptococcus pneumoniae (0.7%), five strains of the Serratia (1.1%), 135 strains of the Escherichia coli (29.7%), one strain of the Alkaligens (0.2%), 46 strains of the Enterobacter (10.3%), 17 strains of the Salmonella (3.8%), one strain of the Arizona(0.2%), two stains of the Citrobacter (0.4%), 14 strains of the Klebsiella (3.1%), 22strains of the Pseudomonas(4.8%) 45 strains of the Proteus(9.9%), two strains of the Bacillus subtilis(0.4%), and six strains of the Clostridium(1.3%). In the case of the blood, 161 strains were isolated from 924 samples out of the culture, and microorganisms of the strains were consisted of 35 strains of the coagulase negative Staphylococci(21.7%), seven strains of the coagulase possitive Staphylococci(4..4%), three strains of the alpha Streptococci(1.9%), one strain of the Streptococcus pneumoniae(0.6%), two strains of the Serratia(1.2%), 56 strains of the Escherichia coli(34.8%), one strain of the Alkaligenes(0.6%), 27 strains of the Enterobacter(6.7%), 17 strains of the Salmonella(10.6%), three strains of the Klebsiella(1.9%), each four strains of the Pseudomonas(2.5%) and Proteus(2.5%), and one strain of the Clostridium(0.6%). In the case of the CSF, 12 strains were isolated from 203 samples, the microorganisms were five strains of the coagulase negative Staphylococci(41.7%), each one strain of the alpha Streptococus, Esherichia coli and Pseudomonas(8.3%), and each two strains of the Streptococcus pneumoniae and Proteus(16.7%). In the case of the thoracic fluid, 23 strains which were consisted of each two strains of the coagulase negative and positive Staphylococci(8.7), one strain of the alpha Streptococcus, Proteus and Bacillus subtilis(4.3%), four strains of the Enterobacter(17.4%), and three strains of the Pseudomonas(13.1%) were isolated out of 80 samples of the cases. In the case of the abdominal fluid, 10 strains which were consisted of each one strain of the coagulase negative and positive Staphylococcus and Bacillus sbutilis (10.0%), five strains of the Escherichia coli(50.0%), and two strains of the Klebsiella(20.0%), were isolated out of 36 samples of them. In the case of the puses, 248 strains which were consisted of 39 strains of the coagulase negative Staphylococci(15.7%), 32 strains of the coagulase positive Staphylococci(12.9%), 23 strains of the alpha Streptococci(9.3%), each two strains of the beta Streptococci and Cilrobater(0.8%), three strains of the Serratia(1.2%), 64 strains of the Esherichia coli(25.8%), 16 strains of the Enlrobacter(6.4%), one strain of the Arizona(0.4%), nine strains of the Klebsiella(3.6%), 14 strains of the Pseudomonas(5.7%), 38 strainis of the Proteus(15.4%), and five strains of the Clostridia(2.0%) were isolated out of 297 samples of the case. The was no meaningful characteristics on the monthly and seasonal differences in the state of the isolation of the microorganisms by the culture, and generally, the Gram negative bacilli were more higher than the Gram positive cocci on the positive rate of the culture for isolation. In the sensitivity tests, the coagulase negative Staphylococci were sensitive against the Novobiocin(NB) in 40% of them, against the Carabnicillin(CBPC) and Lincomycin(LCM) in 30%, against the Kanamyci(KM), Streptomycin(SM), Amikacin(AK), and the Doxycycline(DC) in 20%, against the Penicillin G(PC-G), Erythromycin(EM), Chloramphenicol(CP) and Tetracycline(TC) in 10%, but no sensitive strain in them against the Neomycin(NM). The coagulase positive Staphylococci were sensitive against the CBPC in 40%, the LCM in 30%, the EM, CP, NB in 20%, the NM, AK, DC in 10%, but none in them against the PC-G, KM, SM and TC. The alpha Streptococci were sensitive against the LCM in 50%, the DC in 30%, the EM in 20%, the PC-G, CBPC, KM, CP, TC, NB and AK in 10%, but none against the NM and SM. The Escherichia coli had a sensitivity which was sensitive in 40% against the CBPC, in 20% against the NM and CP, in 10% against the KM, TC, NB, AK and DC, but there was no sensitive strain on them against the PC-G, KM, NM, SM, EM, CP, TC, NB, LCM and AK. The Pseudomonas had no sensitive strain against of all drugstested, The Proteus had a sensitivity which was in 20% against the CBPC, in 10% against the KM, SM, CP and AK, but none in them against the PC-G, NM, EM, TC, NB and DC.

      • 病的資料에서의 病原菌의 分離

        金重明,楊聖基,禹成九,金在植,李健一,金慶淑 慶北大學校 醫科大學 1977 慶北醫大誌 Vol.18 No.2

        1976年 9月 1日에서 1977年 8月 31日까지 滿 1年間 血液外 29種目의 病的資料 2846例中 Micro-coccus外 25種目의 細菌 1763株를 分離하여 그 中 1757株를 同定하였는데 그 結果는 다음과 같다. 血液에서는 Staphylococcus epidermidis (Step.ep.) 41株(36.6%), Staphyloccus aureus (Stap.au.) 7株(6.3%), Alpha Streptococcus (Alpra Str.) 6株(5.4%), Streptococcus pneumoniae(St-r.pneu.) 2株(1.8%), Neisseria meningitidis(N.meningi.) 1株(0.9%), Salmonella group A(Sal.GG.A) 8株(7.1%), Salmonella group D (Sal.G.D.) 13株(11.6%), Escherichia coli (Esch.coli)17株(15.2%), Enterobacter aerogenes (Ente.aero.) 4株(3.5%), Enterobactes cloacae (Ente.cloa.) 2株(1.8%), Serratia marcescens (Serr.marc.) 4株(3.5%), Proteus mirabilis (Prot.mira.) 2株(1.8%), Pseudomonas aeruginosa(Ps-eu. aeru.) 3株(2.7%) 및 未同定 2株(1.8%), 都合 112株를 分離하였다. 裵髓液에서는 總 28株를 分離하였는데, 卽 Stap.ep. 7株(25.0%), Stap. au. 1株(3.6%), Apha Str. 8株(28.6%), Str. pneu. 6株(21.4%), N.me-nigi. 1株(3.6%), Esch.coli 3株(10.8) 및 Proteusvulgaris(Prot. vulg.)와 未同定菌 各 一株(3.6%)였다. 心??液에서는 3株가 分離되었는데 Stap.ep.,Alpha Str. 및 Serr. marc.의 各 1株였고, 肋膜液에서는 23株가 分離되었는데, Stap. ep. 5株(21. 7%), Stap. au.와 Ente. aero. 各 3株(13.0%), Alpha Str. 6株(26.1%), Salmonella arizonae(Sal. ariz.)와 Esch. coli 各 2株(8.7%), Prot.vulg.와 Pseu.aeru. 各 1株(4.3%)였다. 胸廓液에서는 Srap. ep. 3株(50.0%), Srap.au. 2株(33.3%), Prot. vulg. 1株(16.7%), 都合 6株가 分離되었고, 腹水 腹膜液 및 腹腔液에서는 Stap ep. 와 Alpha Str. 各 2株(13.3%), Str. pneu. 3株(20.0%), Esxh. coli 8株(53.3%), 都合 15株가, 關節囊液, 穿刺液 및 囊腫液에서는 Stap. au. 2株(50.0%), Alpha Str.와 Prot. mira. 各 1株(25.0%), 都合 4株가 分離되었다. 尿에서는 Microco., Sal.ariz., Klebsiella pneumoniae (Kleb. pneu.),Ente. cloa., Serr. marc. 및 Serratia liquefa-ciens (Sess. liqu.) 各 1株(0.4%), Stap. ep. 80株(32.9%), Stap. au. 17株(7.0%), Alpha Str.30株(12.3%), Esch. coli 66株(27.2%), Ente. aero. 21株(8.6%), Hafnia 2株(0.8%), Prot.vulg. 13株(5.3%), Pseu. aeru. 8株(3.3%), 都合 243株가, 咽喉에서는 Microco. 62株(24.1%), Stap ep. 28株(10.9%), Stap. au. 13株(5.1%),Alpha Str. 49株(19.1%), Beta Streptococcus(Beta Str.), Str. pneu., Hafnia 및 Serr. ma-rc. 各 1株(0.4%), Esch. coli 33株(12.8%), Ente, aero. 1株(8.2%), Prot. vulg. 3株(1.2%),Conynebacterium diphtheriae (Cory. diph.) 36株(14.0%), Candida albicans (Cand. albi.) 8株(3.1%), 都合 257株가 分離되었다. 鼻에서는 Mi-croco.와 Prot. mira. 各 2株(2.8%), Stap. ep.26株(36.6%), Stap. au. 14株(19.7%), Alpha Str. 5株(7.0%), Esch. coli 19株(26.8%), Ente. aero. 3株(4.2%), 都合 71株가, 喀痰에서는 Micro co. 201株(39.8%), Stap. ep. 25株(5.0%), Stap.au. 9株(1.8%), Alpha Str. 115株(22.8%), Str.pneu. 22株(4.4%), Sal. ariz., Kleb.pneu. 및 Serr. marc. 各 3株(0.6%), Esch. coli 50株(9.9%), Ente.aerc.와 Prot. vulg. 各 13株(2.6%), Ente. cloa., Hafnia 및 Serr. liqu. 各 1株(0.2%), Pseu. aeru. 8株(1.6%), Cand. albi. 37株(7.3%), 都合 505株가, ??에서는 Stap. ep., Stap.au., Sal.G.A., Sal.G.B., Sal.G.C. 및 Serr. liqu. 各 1株(2.1%), Alpha Str. 4株(8.5%), Esch. coli 30株(63.8%), Ente. acro. 2株(4.3%), Prot. mira. 5株(10.6%), 都合 47株가 分離되었다. 膿에서는 Microco.와 Kleb. pneu. 各 2株(0.7%), Stap. ep. 41株(15.2%), Stap. au. 55株(20.4%), Alpha Str. 28株(10.4%), Beta Str.4株(1.5%), Str. pneu., Sal. ariz. 및 Ente. cloa. 各 1株(0.4%), Esch. coli 50株(18.6%), Ente. aero.와 Pseu. aeru. 各 23株(8.6%), Haf-nia 5株(1.9%), Serr. marc. 10株(3.7%), Pr-ot. vulg. 20株(7.4%), Colstridium welchii(Cl-os. welch.) 3株(1.1%), 都合 269株가 傷處排出物에서는 Microco. 와 Hafnia 各 1株(1.9%), Stap. ep. 6株(11.1%), Stap. au.와 Ente. aero. 各 2株(5.6%), Alpha Str. 8株(14.8%), Esch. coli 13株(24.1%), Prot. vulg. 9株(16.7%), Pseu. aeru. 10株(18.5%), 都合 54株가 分離되었으며, 組織片에서는 Stap. ep. 7株(38.9%), Stap. au., Esch. coli 및 , Ente. aero. 各 3株(16.7%), Alpha Str. 2株(11.1%), 都合 18株가, 直腸塗抹物에서는 Stap. ep.와 Alpha Str. 各 7株(10.3%), Esch. coli 34株(50.0%), Ente. aero. 5株(7.4%), Serr. marc. 1株(1.5%), Prot. vulg. 4株(5.9%), Prot. mira. 10株(14.7%), 都合 68株가, 臍帶에서는 Stap. ep. 15株(37.5%), Stap. au.와 Alpha Str. 各 3株(7.5%), Esch. coli 11株(27.5%), Kleb.pneu., Prot. vulg. 및 Prot. mira. 各 1株(2.5%), Ente. aero. 2株(5.0%) 및 未同靜菌 3株(7.5%), 都合40株가 分離되었다. 2種細菌이 同時에 分離된 것이 37例였는데 이 中에서 Microco. 와 Alpha Str.의 混合이 가장 많았고, 3種細菌이 同時에 分離된 것은 8例가 있었는데, M-icroco.와 Alpha Str. 와 Esch. coli의 混合이 가장 많았다. During one year, from 1st of September 1976 to 31st of August 1977, 1757 strains of microorganisms that were consisted of two genera, four groups of Salmonella and 20 species of microorganisms were isolated form cultures of the 2846 cases of the pathogenic specimens that were contained 30 kinds of the clincial materials which were the blood, C.S.F., pericardial fluid, pleural fluid, thoracic fluid, ascitic, peritoneal and abdominal fluids, synovial, aspirated and cystic fluids, urine, throat specimens, nasal specimens, sputum, stool, pus, wound discharges, tissue specimens, rectal swabs and umbilical cords. The results were as follows; 41 strains (86.6%) of Staphylococcus epidermidis (Stap. ep.), 7 strains (6.3%) of Staphylococcus aureus (Stap. au.), 6 strains (5.4%) of alpha Streptococcus (Alpha Str.)., 2 strains (1.8%) of Streptococcus pneumoniae (Str. pneu.), 1 strain (0.9%) of Neisseria meningitidis (N. meningi.)., 8 strains (7.1%) of Salmonella group A (Sal. G.A.), 13 strains (11.6%) of Samonella groupd D (Sal. G.D.), 17 strains (15.2%) of Escherichia coli (Esch. coli), 4 strains (3.5%) of Enterobacter aerogenes (Ente. aero.), 2 strains (1.8%) of Enterobacter cloacase (Ente. cloa.), 4 strains (3.5%) of Serratia marcescens(Serr. marc.). 2 strains (1.8%) of Proteus mirabilis(prot. mira.), 3 strains(2.7%) of Pseudomonas aeruginosa (Pseu. aeru.) and 2 strains (1.8%) of undetermined organisms (Undetermin.), total 112 strains, were siolasted from 845 cases of the blood cultures. In the C.S.F. 28 strains were isolated from 239 cases of the cultures, they were 7 strains (2.5%) of Stap. ep., 1 strain (3.6%) of Stap. au., 8 strains (28.6%) of Alpha Str., 6 strains(21.4%) of Str. pneu., 1 strain (3.6%) of N. meningi., 3 strains (10.7%) of Esch. coli, 1 strain(3.6%) of Proteus vulgaris( Prot. vulg.) and Undetermin. In the pleural fluid, 23 strains were isolated from 85 case of the cultures, they were 5 Stap. ep. (21.7%), 3 Stap. au. and Pseu.Prot.(13.0%), 6 Alpha Str.(26.1%) 2 Salmonella arizonae(Sal. ariz.)and Esch. coli (8.7%), 1 Prot. vulg. and Pseu. aeru. ( 4.3%). In the thoracic fluid, 6 strains of 17 cases were isolated, they were 3 Stap. ep. (50.0%) 2 Stap. au. (33.3%) and 1 Prot. vulg. (16.7%). 15 strains of 34 cases were isolated form the ascitc, peritoneal and abdominal fluids, they were 2 Stap. au. and Alpha Str. (13.3%), 3 Str. pneu. (20.0%), 8 Esch. coli (53.3%). 4 strains of 17 cases were isolated from the synovial, aspirated and abdominal fluids, they were 2 Stap. au. (50.0%), 1 Alpha Str. and Prot. mira (25.0%). In the urine, 243 strains were isolated from 280 cases, they were 1 Micrococcus (Microco.), Sal. ariz., Klebsiella pneumoniae (Kleb. pneu.), Ente. cloa., Serr. marc. and Serratia liquefaciens (Serr. liqu.) (0.4%), 80 Stap. 데. (32.9%), 17 Stap. au. (7.0%), 30 Alpha Str. (12.3%), 66 Esch, coli (27.2%), 21 Ente. aero. (8.6%), 2 Hafnia (0.8%), 13 Prot. vulg. (5.3%) and 8 Pseu. aeru. (3.3%). 2 Microco. and Prot. mira. (2.8%), 25 Stap. au. (36.6%), 14 Stap. au.(19.7%), 5 Alpha Str. (7.0%), 19 Esch coli (26.8%) and 3 Ente. cloa. (4.2%), total 71 strains were isolated form 64 cases of the nasal specimen cultures. In the Sputum, 505 strains were isolated form 298 cases of cultures, they were 201 Micrco. (39.8%), 25 Stap. ep. (5.0%), 9 Stap. au. (1.8%), 115 Alpha Str. (22.8%), 22 Str. pneu. (4.4%), 3 Sal. ariz., Kleb pneu. and Serr. marc. (0.6%), 50 Esch. coli (9.9%), 13 Ente. aero. and Prot. vulg. (2.6%), 1 Ente. cloa., Hafnia and Serr. liqu.(0.2%), 8 Pesu. aeru. (1.6%) and 37 Cand. albi. (7.3%). In the stool, 57 strains were isolated from 181 cases of the cultures, they were 1 Stap. ep., Stap. aul, Sal. G.A., Sal. G.B., Sal. G.C. and Serr liqu.(2.1%), 4 AlphaStr. (8.5%), 30 Esch coli (63.8%), 2 Ente. aero. (4.3%) and 5 Prot. mira. (10.6%). Int he pus, 269 strains were isolated form 300 cases of the cultures, they were 2 Microco. and Kleb. pneu. (0.7%), 41 Stap. ep. (15.2%), 55 Stap. au. (20.4%), 28 Alpha Str. (10.4%), 4 Beta Str. (1.5%), 1 Str. penu., Sal. ariz. and Ente. cloa. (0.4%), 50 Esch. coli (18.6%), 5 Hafnia (1.9%), 10 Serr. marc. (3.7%), 20 Prot. vulg. (7.4%) and 3 clostridium welchii (Clos. welch.) (1.1%). In the wound discharge, 52 strains were isolated form 54 cases of the cultures, they were 1 MIcroco. and Hafnia (1.9%), 6 Stap. ep. (11.1%), 3 Stap. au. and Ente. aero. (5.6%), 8 Aphla Str. (14.8%), 13 Esch. coli (24.1%), 9 Prot. vulg. (16.7%) and 10 Pseu. aeru. (18.5%). In tip and tissue specimesna, 18 strains were isolated from 21 cases of the cultures, they were 7 Stap. ep. (38.9%), 3 Stap. au. Esch. coli and Ente. aero. (16.7%), and 2 Alpha Str. (11.1%). In rectal swabs, 68 strains were isolated from 60 cases of the cultures, they were 7 Stap. ep. and Aphla Str. (10.3%), 34 Esch. coli (50.0%), 5 Ente. aero. (7.4%), 1 Serr. marc. (1.5%), 4 Prot. vulg. (5.9%), and 10 Prot. mira. (14.7%). In the umbilical cord, 40 strains were isolated form 34 cases of the clutres, the were 15 Stap. ep. (37.5%), 3 Stap. au. and Aphla Str. (7.5%), 11 Esch. coli (27.5%), 1 Kleb. pneu. Prot. vulg. and Prot. mira. (2.5%), 2 Ente. aero. (5.0%) and 3 Undetermin. (7.5%). There were 37 cases that two kinds of microorganisms were isolated at the same time and synchronous isolation of the Alpha Str. and Microco. was the most, and were 8 cases that three kinds of microorgainsms were simultaneously isolated and synchronous isolation of Micrco., Alpha Str. and Esch. coli was the most.

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