A New 5α,8α-Epidioxy Sterol from a Marine Sponge Psammocinia Species

Hye Jin Park1,,Xuan Luo1,,Jongki Hong2,,Dong-kyoo Kim3,,Kwang Sik Im1, and,Jee H. Jung1* 한국생약학회 2005 Natural Product Sciences Vol.11 No.4

An investigation of the MeOH soluble fractions of a marine sponge Psammocinia sp. (Order:Dictyoceratida) led to the isolation of a new epidioxy sterol (1) and four known sterols (2-5). Their planarstructures were defined by analyses of the spectroscopic data. The 27-nor-24-methylcholestan type side chain withan epidioxy nucleus (1) was unprecedented. Compounds 1-5 were isolated from a sponge Psammocinia sp. for the first time.

Sulfadimethoxine 투여에 의해 유발된 갑상선 증식성 병변에 나타난 피막 섬유화의 병리학적 특성

김혜성,손화영,조성환,최종윤,윤원기 충남대학교 수의과대학 동물의과학연구소 2001 動物醫科學硏究誌 Vol.9 No.-

Sulfadimethoxine (SM) is one of the anti-thyroid (goitrogenic) agents and has a thyroid tumor promoting effects. In this study, we analyzed the pathology of SM induced thyroid proliferative lesions to see if these lesions showed any distinctive features. Five-week-old male F344 rats were given a single subcutaneous injection of N-bis(2-hydroxypropyl)nitrosamine (DHPN: 2800㎎/㎏, body weight). Starting one week thereafter, SM (1000 ppm in drinking water) was administered for 12 weeks. All animals were autopsied and histopathological observation of the thyroids was perfomed. Thyroid follicular proliferative lesions were induced in all rats treated with SM. In addition, the formation of thick perithyroidal fibrous capsule and pleomorphic follicles within the capsule were observed. These pleomorphic follicles showed invasive figure in some area, especially around follicular adenomas and adenocarcinomas. This confuses adenoma with adenocarcinoma. However, pleomorphic follicles were also observed in the area without adenomas and adenocarcinomas. Therefore, we suggest that it should be careful to diagnose adenoma and adenocarcinoma in the presence of the invasive lesions in surrounding fibrous tissue, especially SM induced thyroid lesion.

새로운 고속 EM 알고리즘

김성수,강지혜 충북대학교 컴퓨터정보통신 연구소 2003 컴퓨터정보통신연구 Vol.11 No.2

본 논문에서는 여러 분야에 사용되는 EM(Expectation-Maximization)알고리즘의 초기치 설정 방법인 K-means의 문제점 해결을 통한 전체 반복 횟수를 줄이는 새로운 EM의 초기값 선정 방법을 제안한다. 이는, 데이터 분포 특성을 이용한 데이터 공간의 균일 분할에 의해, 기존 K-means의 랜덤한 초기값 선정 문제점을 해결함으로써 얻어지는 결과이다. 또한 본 논문은 EM 알고리즘의 초기값 선정 개선방법과 아울러, EM 과정의 핵심을 이루는 후행 확률(Posterior)의 의미를 부각하여 최대 가능성 후행 확률(Maximum Likelihood Posterior: MLP) 과정을 적용한 빠른 수렴특성의 새로운 EM알고리즘을 제안한다. 결과적으로, 새롭게 제안한 EM알고리즘은 기존의 EM 초기값 선정과 수렴에 대한 문제점을 개선함으로써, EM 알고리즘의 특성을 극대화시키는 방향으로 기존보다 더 향상된 결과를 가져온다. 제안된 알고리즘의 객관적 타당성을 위해 기존의 방법과 제안된 방법에 의한 시뮬레이션 결과를 비교 분석하였다.. In this paper, a new Fast Expectation-Maximization algorithm(FEM) is proposed. Firstly the K-means algorithm is modified to reduce the number of iterations for finding the initial values that are used as the initial values in EM process. Conventionally the initial values in K-means clustering are chosen randomly, which sometimes forces the process of clustering converge to some undesired center points. Uniform partitioning method is added to the conventional K-means to extract the proper initial points for each clusters. Secondly the effect of posterior probability is emphasized such that the application of Maximum Likelihood Posterior(MLP) yields fast convergence. The proposed FEM strengthens the characteristics of conventional EM by reinforcing the speed of convergence. Also the number of iteration of searching for the initial values is reduced in great amount, which removes the possibility of falling into local convergence additionally. The superiority of FEM is demonstrated in experimental results by presenting the reduction of iterations of searching for the central points of clustering groups for initial values of EM and accelerating the speed of convergence in parameter estimation procedures..

푸드뱅크 기탁식품의 안전성 확보를 위한 HACCP 제도 적용

김철재,박현신,배현주,이지해,양일선,강혜승 한국식생활문화학회 2002 韓國食生活文化學會誌 Vol.17 No.3

The purpose of this study was to analyze the problems arising from the actual conditions of the Foodbank, and to implement the HACCP system as a solution in terms of increasing the safety of donated food within the Foodbank. In order to apply HACCP system, the entire Foodbank working process such as preparation, collection, transportation, division, and distribution was considered and analyzed to decide the application point for CCPs. Donated foods mainly consisted of processed foods, raw materials, lunch boxes, and cooked foods from mass catering establishments, which dominated over the others in terms of quantity. Cooked foods were divided into three groups based on menu-types and processing methods. Temperature, pH, and aw were measured on cooked foods, and Total Plate Count, Coliforms, E. coli, Salmonella spp., Staphylococcus aureus, Listeria monocytogenes, and E. coli O157:H7 were conducted in order to apply a HACCP plan. Form these experiments, temperature, pH, and aw of donated food were likely contributed to microbial growth. Donated foods before HACCP implementation showed high numbers in terms of total plate count and Coliforms, both well over the acceptable standard levels. By setting the CCPs on maintenance of donated food below 10℃ and using a 75 ℃ reheating method, microbiological hazard levels were able to be controlled and lowered. From these results, it is concluded that in order to guarantee food safety, foods donated to the Foodbank must not only maintain a reasonable level of initial microbiological growth, but also must be handled properly through time and temperature controls within the Foodbank system. Furthermore, in terms of implementing the HACCP plan within the Foodbank management structure, basic food safety and sanitation measures, such as reheating facilities and various cold chain systmes such as refrigerated vehicle for food transportation are importantly needed. The training and education of Foodbank personnel and management in areas such as awareness of hygiene and safe food handling and practice are also required and necessary.

발아현미가루를 첨가한 머핀 제조조건의 최적화

김철재,이지해,이은혜,문혜진,주나미 숙명여자대학교 건강·생활과학연구소 2006 生活科學硏究誌 Vol.- No.22

In order to develop the functional muffin prepared with different levels of sprouted brown rice flour (SBRF), the mixing conditions of SBRF, butter and water were optimized by response surface methodology(RSM), using central composite design with 3 variables and 5 levels. The result of three-dimensional plot was showed that decrease of SBRF content increased the volume, maximum length, crumb grain, moistness and overall quality of muffin. The overall quality of the functional muffin was maximized under the preparing condition of 31.49 g SBRF(15% of flour used), 85.36 g butter, and 123.86 g water. Moreover, the optimum conditions for preparing muffin were as follow : 29.95 g, 81,24 g, 120.45 g for volume ; 23.67 g, 88.18 g, 118.24 g for maximum length ; 38.44 g, 63.38 g, 117.26 g for flavor ; 29.86 g, 93.04 g, 126.42 g for taste ; and 55.43 g, 94.40 g, 133.61 g for moistness. The range of optimum preparing conditions for the functional muffin in consideration of the physical and sensory characteristics were shown to the 30~40 g as SBRF, 80~90 g as butter, and 120~130 g as water.

코로나19 대응을 위해 한시적으로 허용된 전화상담·처방에 대한 병원급 의료공급자 인식과 만족도에 관한 연구

유혜림,김수민,박춘선,김지애 건강보험심사평가원 2021 HIRA RESEARCH Vol.1 No.1

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환경보고서의 유용성에 대한 이용자의 인식에 관한 설문조사연구

장지인,김장환,정혜정 대한경영학회 2003 大韓經營學會誌 Vol.16 No.7

This study will develop the way of working out the more useful environmental report based on the result of the survey which was carried out in the companies currently publishing the report(publisher) and stakeholder(report user). At present, the number of domestic company which publishers the environmental report is quite few, however, its number will be expected to reach high in near future in the light of its importance. In the survey of usefulness of those environmental reports, 24 out of 25 report publishers and 18 out of 22 report users answered "useful". However, both of publishers and users pointed out the various points at issue of their points of views when they wrote and used the reports. First of all, the publishers usually make out the environmental reports based on the guideline out of the Ministry of Environment which is official recognized standard to help the publishers the consistency of the report and easiness of making out. However, the publishers also indicate controversial points of impeding the publisher's creativity due to its too much generalization. The users of those environmental reports present the problems of the impossibility of making comparison between entities due to the difficulties of the substance of the report, credibility of its objectivity and confidence, and lack of unified formation of report. The result of this survey is expected to be good reference for the development of the environmental report.

복수과 진료 환자의 중복 처방에 대한 분석 및 평가

이지은,장혜경,오지영,유윤경,김현지,임숙인,연숙희,강진숙,최귀령 한국병원약사회 2003 병원약사회지 Vol.20 No.1

In an aging society, there is an increasing possibility of the duplication of the drugs given to patients because they take many kinds of drugs. Among the prescriptions given to the patients who was treated at multiple ambulatory clinics in St. Mary's hospital for one month of September, 2002, in which drugs that has identical or similar effects are prescribed, we analysed and evaluated them by patients' characteristics, kinds of medication, severity of side effects on a case by case basis. More, we assessed the potential additional costs. As a result of this study, the duplication rate turned out to be 6.69% and it was shown that the proportion of the elderly patients over 60 was high. The gastro-intestinal medications took up a large part and most of the cases showed that the danger caused by double taking of medicine was slight, but there were some examples in which the serious side effects were predicted. Expected additional costs were the average 11.349 won and it ranges from the minimum of 56 won to the maximum of 135,720 won. In conclusion, the management of drug histories of the patients who need the plural treatments is very important and the necessity of the individual and professional guidance of taking medicines for the elderly patients is emerged, too.

병원내 안전관리 향상을 위한 항목 및 지침 선정

박지원,김용순,진혜영 한국의료QA학회 1996 한국의료질향상학회지 Vol.3 No.1

Background : The goal of this study was to establish the QA items and guidelines for preventing and improving of safety management. Therefore we investigated the nurses' recognition and knowledge of the safety and risk procedures and policies, and the agreement between the nurses beliefs on the degree of miportance of those procedures and policies, with actual implementation in hospitals. Method : The subjects of this study were 201 nurses who participated in a program called continuing education for nurses, which held in December, 1993. Result : The results of this study were as follows: 1. Among 18 types of hospital risks, the items that scored highest or the need of closer attention in safety management was the needle stick, medication errors, falling, and bed sores. 2. In most questions of the 18 incidences, the nurses showed that the estimated result would have positive signs except for hospital infections, burns, and bed sores. 3. Even though the survey shows that incidences and types of occurences varies according to the person's age and the time of incident, they mostly occur between midnight to 6AM. Falls and bed sores can be seen more in the elderly. Medication errors, hospital infections and bums are frequently found between the ages of one through twenty. 4. There was a higher mean score for recognizing the importance of those items than the importance of implementing them. Conclusion : In summary, nurses did perceive the need of safety management but the hospital policy for proper safety management was not established. So we recommended that the hospital administration would undertake an early detection and proper management system for hospital precautions, based on QA items & guidelines presented in this study.

사람-사람 하이브리도마의 생산을 위한 fusion partner cell의 개발

안지영,김혜원,서동상 성균관대학교 생명과학자원연구소 1995 生命資源科學硏究 Vol.2 No.1

This study was performed to establish human hybridmyeloma cell lines as fusion partner cells for producing human-human hybridoma. Human myeloma, SKO-007(J3), and human cervix epitheloid carcinoma cell line, HeLa MR, were fused by using with 50% PEG, and cultured in RPMI 1640 medium containing 15% FBS. Hybrids of SKO-007(J3) and HeLa MR were selected in RPMI 1640 media containing 6-thioguanine(6-TG ; gradually increasing concentration from 10 ㎍/ml to 20 ㎍/ml, and then gradually decreasing to 10 ㎍/ml) for 6 weeks. 6-TG resistant cells were collected and cloned by limiting dilution method. The fused myelomas with HeLa MR grew more rapidly than the fused cells with a small number of HeLa MR. Their doubling time was 72 hours.