Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.

레크리에이션 활동이 정신지체아의 사회적 기술과 문제행동에 미치는 효과

강병일,추근도,최경훈,박기용,박병규 한국특수체육학회 2004 한국특수체육학회지 Vol.12 No.3

The purpose of this study was to identify the effect of recreation program applied to the mentally retarded children on their social skill and problem behavior. The subjects of the study were the mentally retarded children who were attending at J middle school for special education in Andong-city, Kyungpook Province and the children were divided into two groups; experimental and comparative groups. Research design was as follows; the input of recreation activities was designated as independent variables, male and female mentally retarded children group was determined as object variables, and social skill and problem behavior was subject variables. After the pre-tests on the scales of the sampling children's social skill and problem behavior were carried out, experimental group was applied to the recreation program and comparative group was taught as a usual time and then the post-tests was performed. t Examination was used to identify the differences according to the results from post-tests and the data were processed by SPSS 10.0(Statistical Package for the Science) statistics program. The results from the above research were as follows; First, the mentally retarded children applied to the recreation program had a positive effect on their social skill. Second, the application of the program also had a positive effect on their problem behavior. From the results of this study, my suggestions were First, the follow-up research should be provided to identify the effect of recreation program on the children being various levels. Second, the range of research should be expanded, so the consistent research on children of kindergarten or after-elementary school should be followed. Third, the succeeding research on the long-term transfer of the program's effect should be continued. Forth, other programs(or strategies) could attribute to the promotion of the mentally children's social skill and the improvement of their problem behavior. Fifth, the research capable of inducing their activities after understanding the students' traits, interest degree on the subjects and so on should be made. Finally, each agencies or schools should develop and apply more systematic recreation program and also try to develop a system which could be classified and applied to recreation fields suitable for the individuals' traits.

모 축전지 회사 근로자들의 연폭로 지표에 대한 경시적 관찰

강명식,김용배,이용진,리갑수,김화성,황규윤,장봉기,이성수,안규동,이병국 순천향대학교 산업의학연구소 1998 순천향산업의학 Vol.4 No.1

Lead poisoning is still prevalent and one of important occupational disease in Korea. To prevent undue high lead burden and maintain good health condition of lead workers in lead using industries, periodic health examination and regular biological monitoring of lead workers are often required in addition to the fundamental improvement of their working condition. To evaluate the effect of comprehensive occupational health program with regular biological monitoring on the level of lead exposure of lead workers in lead using industries, 11 year database from 1983 to 1994 of one storage battery factory was reviewed. From 1983 to end of 1986 only periodic health examination was provided to the lead workers, but from 1987 comprehensive occupational health program with regular biological monitoring was provided to maintain better health condition of lead workers. Selected study variables for lead exposure were blood lead(PbB), zinc protoporphyrin in blood(ZPP), δ-aminolevulinic acid in urine(ALAU), hemoglobin(Hb), hematocrit(HCT). Information on sex, age and work duration was also reviewed. 5 years data from 1983 to 1987 and 3 years data from 1990, 1993 and 1994 were available for all lead exposure indices except ALAU which has only 5 years data from 1983 to 1997. The results of 11 years longitudinal observation of lead indices are all follows: 1. The mean value of ZPP of lead workers in 1983 was 148.7±73.1 ㎍/㎗(Male: 145.5±72.3, Female: 168.1±79.1), whereas that of ZPP of lead workers in 1994 was decreased to 64.3±32.7 ㎍/㎗(Male: 60.2±28.8, Female: 95.2±43.4). 2. The mean value of PbB of lead workers in 1983 was 56.4±18.8 ㎍/㎗(Male: 55.59±19.1, Female: 59.6±19.1), whereas that of PbB of lead workers in 1994 was dcreased to 35.4±11.9 ㎍/㎗(Male: 35.7±12.0, Female: 32.8±10.8). 3. The mean value of Hb of lead workers in 1983 was 14.1±1.4 g/㎗(Male: 14.4±1.1, Female: 11.7±0.8), whereas that of Hb of lead workers in 1994 was increased to 15.6±1.5 g/㎗(Male: 15.9±1.2, Female: 12.9±1.1). 4. Blood lead is most highly correlated with ZPP(r=0.522, male:0.54 and female:0.50) and also correlated with ALAU and work duration also. 5. The simple regression analysis of PbB and ZPP showed different coefficient of regression by the measurement year, but there was no difference of coefficient of regression between sex. The slope of equation of pooled data for 1983-1986 without comprehensive occupational health program was 2.16 and differed from that of pooled data for 1987-1994(1.25). This difference suggests that the unit effect of blood lead on ZPP was relatively smaller in later period of comprehensive occupational health program than carly period without comprehensive program. The slope change in the analysis of linear regression of PbB on ZPP in lead workers can be used to evaluate the possible effect of health intervention to prevent undue high lead absorption in lead using industries. 6. 11 years occupational health service in this storage battery factory brought out considerable improvement of health status of lead workers in terms of decrease of PbB and ZPP and increase of Hb and the improvement was more remarkable after the introduction of comprehensive occupational health program.

준설수심에 따른 사천만의 해수유동 특성에 관한 연구

강대석,유상호,민일규,이동수,민병형 東亞大學校 海洋資源硏究所 2003 硏究論文集 Vol.15 No.-

This study on the characteristics of the tidal current at the half closed bay selected Sachoˇn Bay located in the western Kyung-nam area as the object location of the study. Then, I analyzed the current with the varying dredging depth after the depth integral of the three Dimensional continuous equation and Navier-stokes equation. The study would get the following results, and the results could be used meamingfully as the analysis material about the dredging depth's influnce on the surrounding ocean when developing the coastal port. 1. When measuring the tidal current, there is a bit dispersion in the current direction of the observed object, but it is the wind effect at the bay. Considering that the calculated result is the shallow-water equation integranted in depth, the results can be concluded to in accored with my expectancy. 2. In case of reclaiming and dredging, the flow velocity and direciton change constantly, compared with the present condition. 3. At the wide bay dredge depth does not arrect the level of the ocean, but in case of inflow from the flood, the increase of the inflow of the river water affects the level of the ocean. 4. As the dredge depth grows deep, the maximum flood tidal current and obb tidal current at the front coast of the reclaimed decreases a little. However, the wide area Pc-7~Pc-9 doesn't show any change. As we saw in the above result, feacturcs of the tidal current as the level of the ocean and the current velocity don't change in the aspect of the ocean engineering even if we increase the degree of the dredge depth up to 6m in Sachoˇn Bay

악성 갑상선 우연종의 임상상 및 초음파 소견의 진단적 가치

강한욱,김광원,이병완,강보현,김형훈,김범진,정재훈,민용기,이명식,이문규 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.5

연구배경: 최근 갑상선 초음파 검사의 빈번한 시행으로 갑상선 우연종의 발견이 증가하고 있다. 이러한 갑상선 우연종은 임상적으로 어떻게 접근하고 치료를 해야 하는지 적지 않은 문제가 되고 있다. 본 연구에서는 직경 1.5㎝ 이하의 갑상선 우연종의 유병률, 입상적 특징 및 초음파 특성, 그리고 가장 효율적인 진단적 접근에 대해 알아 봤다. 방법: 1999년 1월부터 2000년 12월까지 삼성서울병원 내과 및 일반외과로 갑상선 결절을 주소로 내원한 1457명을 대상으로 후향적 연구를 시행하였다. 우연히 발견된 직경 1.5㎝ 이하의 갑상선 결절을 가지는 환자들에서 갑상선 결절의 촉지 유무, 갑상선 기능검사, 항갑상선 항체, 갑상선 초음파 및 미세침 흡인세포검사, 99m^Tc 갑상선 스켄, 수술 조직검사 및 병기를 검토하였다. 결과: 분석한 갑상선 결절 1475예 중 갑상선 우연종의 유병률은 13.4%(198예)였다. 갑상선 우연종에서 악성률은 28.8%(57예)으로 나왔다. 양성 및 악성 갑상선 우연종에서 나이, 성별, 갑상선 기능검사, 결절 크기에는 의미 있는 차이는 없었다. 갑상선 스켄은 갑상선 우연종의 감별에 적합하지 않았다. 갑상선 결절의 초음파 지표 점수만이 양성 및 악석 갑상선 우연종의 감별에서 초음파 지표 점수의 민감도는 88.9%, 특이도는 74.4%, 양성 예측률은 92.4%로 나왔고 진단적 정환도는 85.5%였다. 갑상선암으로 수술을 받은 50명중 stage 1은 34명, stage 2는 6명, stage 3은 10명으로 대부분의 악성 갑상선 우연종은 낮은 TNM 병기로 나타났으나, 상당수에서는 국소 침범의 소견을 보였다. TNM 병기의 3기 환자 전부는 45세 이상이였고, 주로 갑상선암의 피막 침범에 의한 것이었다. 임파절 전이는 6명의 환자에서 나왔다. 결론: 악성 갑상선 우연종은 비교적 흔히 접할 수 있는 질환이며 양성 우연종과는 임상적으로 구별이 안 된다. 악성 갑상선 우연종은 발견 당시 대부분 낮은 병기이나, 갑상선의 피막 침범 및 임파절 전이가 상당수에서 발견되었다. 갑상선 우연종의 초음파 소견은 향후의 진단 방향을 제시하는 중요한 길잡이가 될 수 있다. 초음파 지표 점수 2점 이상의 갑상선 우연종에서만 초음파 유도 미세침 흡입검사를 권장한다. Background: High-resolution ultrasonography has made the detection of asymptomatic small thyroid possible. Recent increases in the detection of incidentalomas have created a clinical dilemma on how to properly manage such incidental nodules. We investigated the prevalence, clinical and ultrasonographic characteristics, and optimal diagnostic approach toward incidentally detected benign and malignant thyroid nodules of less than 1.5㎝ in size. Methods: A retrospective review was undertaken on the 1,475 patients who had visited Samsung Medical Center, Seoul Korea between January 1999 and December 2000. The review consisted of a physical examination of the thyroid gland, thyroid function test, antithyroid antibodies, thyroid ultrasonography, fine-needle aspiration biopsy, pathology and TNA staging of the incidentally detected thyroid nodules of less than 1.5㎝ in size. Results: The prevalence of thyroid incidentalomas was 13.4% and the malignancy rate within them was 28.8%. There were no significant differences in age, sex, thyroid function test and size between the benign and malignant incidentalomas. Ultrasonographic characteristics showed meaningful diagnostic value for the detection of malignancy in incidentalomas. Most malignant incidentalomas were of a low stage. Conclusion: Occult thyroid cancers are fairly common finding. There are no clinical difference between benign and malignant thyroid nodules less than 1.5㎝; however, ultrasonographic findings can be used to decision of optimal management strategies (J Kor Soc Endocrinol 17:649∼656, 2002).

全南地方의 韓牛에 發生하고 있는 原因不明의 急性疾患에 關한 硏究(豫報)

康炳奎,金廣植,李政吉 全南大學校 農漁村開發硏究所 1973 農業科學技術硏究 Vol.7 No.-

Studies were made on the etiology of the acute, unknown disease of Korea cattle in Chonnam area. Total 12 cattle reared in the infected area were selected and examined periodically from May to November in 1972. Of the 12 cattle, two were died during the experiment. They were autopsied and histopathologically examined. In order to compare with symptoms and histopathological changes of died cattle, three goats were poisoned with phenylmercuric-triethanol-ammonium borate(PTAB), sodium nitrate(NaNO₃), and potassium cyanide(KCN). Meanwhile, samples of water, soil, and feedstuffs from the infected and noninfected area were analysed. Results obtained and matters presumed were as follows: 1. Chemical examinations for blood revealed that Korean cattle reared in the infected area had pronounced hypomagnesemia and hypocalcemia. The total leukocyte count was gradually increased in the blood examination of the two Korean cattle died during the experiment. In the analysis of soil, water, and feedstuffs of the infected and noninfected area, the differences were noted in the values of magnesium, calcium, and nitrate nitrogen. 2. Similar histopathological changes of liver and kidney in died cattle and poisoned goats led us to suspect a toxic substance. 3. Next studies should include the blood examination for calcium, magnesium, nitrate and nitrite nitrogen, cyanide, copper, vitamine A, and methemoglobin. Further investigations on soil, water, and feedstuffs are also desired.

칡의 部位別 抗酸化 作用에 關한 硏究

林圭,朴涌基,康秉秀 대한본초학회 2001 大韓本草學會誌 Vol.16 No.2

The Parts of Pueraria thunbergiana Bentham, Puerariae Flos(PF), Puerariae Radix(PR), Puerariae Leaf(PL), Puerariae Sprout(PS) were investigated effects of for DPPH(electron donating ability) radical ability, nitrate reductase activity(NR), superoxide dismutase activity(SOD), catalase activity, lipid peroxidase(LP) and glutathione(GSH). In vitro, DPPH radical ability and nitrate reductase activity(NR) were increased according to the increasing of PF, PR, PL, PS concentrations. Among others PF, PR were significantly increased to the level of BHT. In vivo, compared with normal group, SOD(superoxide dismutase) activity and catalase activity were decreased in the acetaminophen treated group. On the other hand, PF, PR, PL, PS in the pretreated groups SOD activity and catalase activity were significantly increased. Among others PF, PR were almost increased to the level of Ascorbic acid. Compared with normal group, lipid peroxidation was increased. Among others PF, PR, PS were almost significantly decreased to the level of Ascorbic acid. In the acetaminophen treated group, GSH activity was decreased by compare with normal group. And in the PF, PR, PL, PS pretreated groups, GSH activity was increased to the control group. Among others PF, PR were almost significantly increased to the level of Asorbic acid. These results suggest that among others PF was almost increased to the level of Ascorbic acid.

생체분해성 고분자를 이용한 사시수술용 제형개발 및 효용성에 대한 연구

민병무,김용백,김승영,김창식,박근성,길숙종,조항진,이성복,노승무,송규상,강대영,조준식,양준묵,정경수,최선웅,이진호,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

A new device, muscle clamping system was developed to facilitate exact quatifying technique A strabismus surgery, and reduce the risk of complications. The device is composed of a lower fixing body with three jaws and an upper supporting body. They are used to clip an extraocular muscle and fix it to the sclera with a single bite. Superior rectus recession on 16 rabbit eyes were performed with this new device. Conjunctival injection, muscle adhesion strength, and light microscopic findings were examined at 1, 2, 4 and 8 weeks postoperatively. The Conjunctival injection were minimal, adhesion power ranged from 420 to 600 gram gravity, which is sufficient in withstand the normal pull of human extraocular muscle. In microscopic exam, some Inflammatory cells and fibrosis were found. The new device was technically easy, fast, and accurate, so it may be useful in stabismus surgery.

백서의 복강내에 투여한 Mitomycin-C-Polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

송규상,강대영,최정목,노승무,정경수,오정연,김진향,양준목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

To evaluate the in-vivo effects of mitomycin-C-polyglycolic acid composite(MMC-PGA) the disk: shaped composite were implanted into the peritoneal cavity of the Sprague-Dawley rats. The pathologic changes were examined at various time points up to 12 weeks. Initially the soft tissue around the inserted disks showed capillary congestion at 3 days. Inflammatory infiltrates with foreign body giant cells appeared from the 2nd week and reached peak response at 6-8 weeks. These reaction diminished prominently at 12 weeks. No specific pathologic change was found in the liver, the kidneys, and the heart. The above results suggest that the MMC-PGA composite can serve as a new device for intraperitoneal chemotherapy of various types of cancers.

방파제 평면 배치에 따른 항내 정온도에 관한 연구

민병형,민일규,이정환,강성기 동아대학교 건설기술연구소 1999 硏究報告 Vol.22 No.2

The harbor Calmness decides is hydraulic and numerical medel test. The study is Hongdo harbor building of accompany that hydraulic and numerical motel test as breakwater plan arrangement for analysis of in harbor clmness and The harbor operate rate as invsetigates is result as numerical model test. This summarize shoes the following result 1. The analysis result is nearly agreed of hydraulic and numerical model test. 2. The analysis result is case.4 is very calmness of hydraulic and numerical model test and case.1 is failed, case.2, case.3 of campared as case.3 of judged that more calmness of arrangement all borken course. 3. The analysis test results of the harbor operate rate case.1 is samall then another cases that critical wave height of anchor area but the calmness of anchor area of the case.1 that is much broarder than Hongdo anchor area therefor case.1 was decided no problme that ship is used harbor. This study result will be used of Hongdo harbor in the future for plan building of breatwater. The future for hardof high exactly as hydraulic and numerical model test especially, the will be studied numerical model test for beach reflection, breaking wave height, submerged friction.