多段階 在庫시스템에서의 最適 安全在庫 模型

沈宰弘,南相進,金正子 동아대학교 공과대학 부설 한국자원개발연구소 1995 硏究報告 Vol.19 No.1

This research proposed a model for the determination of optimal safety stock for multi-echelon inventory system. The criterion is to minimize expected anaual total cost safety stock of system under service level constraint. The results of numerical examples for three-echelon inventory system are analized and compared with Salameh and Schmidt's model. The heuristic method is presented to find an optimal-tending solution for total variable system cost per year. The numerical examples are illustrated for verification and validation purposes.

紅景天(Rhodiola sachlinensis)에서 항균성 물질의 분리 및 동정

심창주,이규희,정재홍,이상덕,김영호,오만진 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2004 한국식품저장유통학회지 Vol.11 No.1

홍경천의 천연 보존료로서의이용성을 검토하기 위하여 각종 용매로 홍경천 추출물을 제조하고 극성에 따라 순차분획, silica-gel chromatography, thin-layer chromatography, high perfomance liquid chromatography의 분리과정을 거쳐 순수 분리된 각 물질을 GC/MS(EI) spectrum, ¹H-NMR spectrum, ¹³C-NMR spectrum을 이용하여 항균물질을 동정하여 다음과 같은 결과를 얻었다. 홍경천에 대해 acetone, ethyl acetate, ethanol, methanol, chloroform, water의 각 용매로 추출한 결과 항균성분의 추출 용매로는 methanol이 가장 우수하였다. 홍경천 methanol 추출물을 n-hexane, chloroform, ethyl ether, ethyl acetate, n-butyl alcohol로 분획한 결과 ethyl acetate와 n-butyl alcohol 분획에서 우수한 항균력을 나타내었다. 홍경천 methanol 추출물은 그람양성 4균주와 그람음성 4균주에 대해 고체 배지의 경우 대체로 500 μg/mL에서 최소저해를 나타냈으며, 액체배지의 경우 100 μg/mL 내외에서 최소저해를 나타내었다.] Silica gel column chromatography에서 용매의 배율을 높여가며 용출한 결과 chloroform : methanol(8:2, v/v)의 비율에서 항균력이 가장 높게 나타났다. 분리된 항균성분을 silica-gel TLC plate에 용매의 비율을 높여가며 전개시켜 chloroform : methanol(9:1, v/v)에서 9개의 물질군을 얻을 수 있었으며, 그 중 항균력이 확인된 3개의 물질군을 HPLC와 NMR spectrum을 분석한 결과 gallic acid, (-)-epicatechin 및 kaempferol로 동정되었다. gallic acid, (-)-epicatechin 및 kaempferol의 항균물질 각각의 최소저해농도는 Staphylococcus aureus와 Listeria monocytogenes에 대해 gallic acid와 kaempferol 이 500 μg/mL, (-)-epicatechin이 100 μg/mL에서 저해를 나타내었다. The antimicrobial substances from Rhodiola sachalinensis were extracted, isolated and identified. The highest level of antimicrobial activity and its yield were obtained in methanol extract. The minimum inhibition concentrations of the methanol extract were 500 μg/mL on agar plate and 100 μg/mL in broth media for four gram positive and four gram negative microbials. The methanol extract was fractionated by n-hexane, chloroform, ethyl ether, ethyl acetate, and butanol, orderly. The separate was developed on the TLC plate with different solvent system ratio of chloroform and methanol. Nine substances were isolated from chloroform and methanol mixture(9:1, v/v). Among them, three isolates showed antimicrobial activity. Three substances separated by HPLC were identified by GC/MS(EI) spectrum and ^(1)H, ^(13)C-NMR spcetrum. They were gallic acid, (-)-epicatechin and kaempferol. The antimicrobial activites of each substances were shown gallic acid, (-)-epicatechin, kaempferol, orderly.

TNF-α 유전자로 형질전환된 효모의 성장양태 및 단백질 생산

심두희,이진옥,이동근,이재화 신라대학교 자연과학연구소 2004 自然科學論文集 Vol.13 No.-

사람 TNF-a를 생산하도록 GPD promoter와 AG promoter로 형질전환된 S. cerevisiae가 보이는 성장양상, 총분비단백질, pretease 활성을 측정하였다. GPD promoter로 형질전환 된yeast는 63.6 g/L의 성장이 일어났고 총분비단백질은 9 mg/L이며, pretease 활성은 2.55 X10^(4) unit/L 였다. AG promoter로 형질전환된 yeast의 경우엔 68 g/L의 성장이 측정되고 총분비단백질은 27 mg/L, pretease 활성1.55 X10^(4) unit/L였다. 단백질과 protease 분비량은 세포의 성장과 비례하는 것을 알 수 있었고, GPD promoter로 발현되는 TNF-a의 양은 배양초기에는 검출 한계 이하였고 15시간 이후에 증가하는 양상을 보여 배양 후 35시간째에 가장 많은 양 약 2535 ng/ml 정도가 분비되는 것으로 나타났다. We measured growth, total secreted protein and total secreted protease of transformed Saccharomyces cerevisiae with human TNF-α gene. Two different promoters, GPD promoter and AG promoter, were used for heterologous expression of human TNF-α gene. The growth which transformed TNF-α:GPD promoter was 63.9 g/L and total secreted protein was 9mg/L, protease activity was 2.55 x 10^(4) unit/L. In another case at TNF-α:AG promoter the growth was 68 g/L, total secreted protein was 27 mg/L and protease activity was 1.55 X 10^(4) unit/L. We could know total secreted protein and protease were directly proportional increase with growth. In initial time of culture, we could not see the TNF-α content in GPD promoter but after 15hours the yeast produced TNF-α. The highest level of product of TNF-a was 2535 ng/ml at 35hours after culture.

‘융복합교육’ 기반 수업 설계 사례 연구 : 예비 교사를 중심으로

심수진,박재현,최수빈,안성호 이화여자대학교 교과교육연구소 2016 교과교육학연구 Vol.20 No.4

본 사례 연구는 융복합교육의 현장 실천과 적용가능성 및 교사의 융복합교육 전문성 제고에 기여하고자, 한 중학교 현장에서 교생실습의 일환으로 자생적으로 행해진 예비교사들의 융복합교육 기반 수업 설계 프로젝트를 분석한 것이다. 한 교사의 주도 하에 21명의 예비교사들이 다섯 개 모둠으로 나누어 협업함으로써 융복합수업을 설계하였는데, 본 논문에서는 그 교사의 서면 인터뷰와 예비교사들의 수업지도안, 발표, 전문가 컨설팅, 성찰지를 자료로 취합하여 정성적으로 분석하였다. 특히 융복합수업 지도안에 대해서는 이전에 체계화되어 있는 융복합교육 프로그램 구성 틀을 바탕으로 분석하였다. 연구 결과, 수업지도안은 각각 창의적으로 다양하게 융복합교육적 목표와 방식 및 맥락을 충분히 잘 반영하였으며 주제, 내용, 활동요소 등도 융복합에 부합하게 유기적으로 연결된 것이 발견되었다. 단, 자율적 실천 역량을 더욱 촉진하고 개인에서 세계로까지 맥락을 확대하며 이를 다채로운 활동들을 혼합하여 구성할 것이 요구되었다. 한편, 성찰지에 의하면, 이번 사례에 대해 예비교사들은 1) 융복합교육을 ‘과목 간 연계성’, ‘수업의 맥락화’, ‘학습자 중심’으로 차별화하였으며, 2) 융복합수업 설계를 하면서 얻은 ‘새로운 경험’을 통해 자기 과목에 대한 새로운 측면, 학생의 관점, 동료의 전문성, 그리고 협업을 가치 있는 것으로 성찰하였다. 3) 또한, 융복합교육의 절대적 필요성을 인식하고 교사 전문성 신장과 연구자 자세 함양을 되새기며 향후 융복합교육을 실천하겠다고 진술하였다. 이에 따라 본 연구는 현장의 교육 개선을 위해 교사의 재량권 보장과 행정적 지원이 필요함과 융복합교육과 관련하여 (예비)교사교육의 체계적이고 실증적인 개선이 필요함을 시사한다. To explore the implementability of, and the teacher expertise in “Yungbokhap education”, which embraces “consilient”, “convergent”, “integrative”, and “interdisciplinary” approaches to curricular integration, this case study analyzed a self-motivated project for pre-service teachers that was initiated as part of a practicum by a middle-school teacher who supervised the practicum. In the project, the pre-service teachers formed five groups and collaborated to create five integrative instructional unit plans based on Yungbokhap education. Their products positively impressed the school principal, mentor teachers, and other experts. They wrapped up their projects by answering reflective questions. This study analyzed their instructional unit designs and reflections qualitatively, in light of the instruction design framework of Yungbokhap education. The results show that the unit plans sufficiently reflect the goals, approaches and contexts of Yungbokhap education with appropriate creativity and variety, and tightly connect lesson themes, contents and activities, except for the fact that they could have further promoted the competency for autonomous action and expanded the context from an individual context to a local/global one employing more varied activities. According to their reflective responses, 1) they seem to characterize Yungbokhap education with features like “inter-subject connectivity”, “contextualization” and “learner-centeredness”, 2) their novel experiences in the lesson planning have led them to discover fresh values in their own subjects, students’ perspectives, peers’ expertises, and fruitful collaboration, and 3) their realization of the necessity of Yungbokhap education has positively challenged them to be more firmly oriented towards professional development and teacher-researcher attitude. These results imply that for autonomous and creative improvement of education, in-service teachers’ initiatives should be carefully respected and administratively supported, and that teacher preparation courses should provide pre-service teachers with more empirical chances to deal with curriculum integration.

간내 간충병에 대한 간절제술 1예

심민철,김홍진,권광보,노성균,이헌주,이태숙,장재천 영남대학교 의과대학 1990 Yeungnam University Journal of Medicine Vol.7 No.1

영남대학교 의과대학 외과학교실에서는 1989년 11월에 한국에서 발병한 간흡충병의 여섯 번째 증례로, 특히 간내 담관에 발생한 첫 증례를 우측 간엽 절제술 및 담낭 적출술로 좋은 결과를 얻었으며, 간내 담관에 발생한 경우에는 간암과의 구별이 요구되어야 한다는 점을 상기 시키면서 증례보고와 더불어 문헌적 고찰을 하였다. Human Fasciola hepatica infection is a rare entity involving infestation of the liver and biliary tree with adult flukes, which can result in hepatitis, cirrhos is and biliary tract inflammation, obstruction and lithiasis. The patient had the typical diagnostic tetrad of fever, eosinophilic leukocytos is, tender hepatomegaly and fluke ova in the stools. Theatment consistes of Emetine hydrochloride administration for hepatic involvement and common bile duct exploration for removal of flukes, with cholecystectomy for associated cholelithiasis. The combination of medical and surgical therapy cal be expected to produce an arrest of this infection. The removed liver revealed eggs of the fasciols species in the intrahepatic bile duct. The clinical history, pathological findings and treatment of this case were described.

Preloading과 Sand drain에 의한 軟弱地盤 改良에 關한 考察

沈泰燮,安鍾弼,柳在珍 조선대학교 국토개발연구소 1987 국토개발연구 Vol.7 No.1

The Most Effective soil Improvement Method for Homogeneous thick clay stratum is preloading Method with sand drain. Selecting four subsoil strata, It has been Analyzed and measured for soil parameters, Subsoil characteristics. Predicted value and obserbed value in field was both used for the calculation's Analysis of this study, Consolidation degree-Time curve, field Settlement-Predicted Settlement, Secondary Settlement by Surcharging Loading It was found that preloading method with sand drain's a highly effective soil Imp개rement Technique through Investigation of time reducing Quantity Calculation and Soil characteristics change Quantity. The Research for this sector should be proceed more and more.

진행성 위암과 동반된 후복막 섬유화증 7예의 임상적 고찰

장재영,천영국,최우봉,김진오,조주영,이준성,이문성,심찬섭 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

Background/Aims: Retroperitoneal fibrosis is a fibrosing disease process which frequently develops ureteral obstruction. Malignant retroperitoneal fibrosis has been reported that various types of cancer including cancer of the breast, stomach, prostate, lung, cervix, uteri, colon, pancreas, ovary, and even Hodgkin's disease. While the pathogenesis remains obscure, small foci of metastatic neoplasm in the retroperitoneal space can elicit a desmoplastic reaction in secondary form to malignancy. Methods: Recently we experienced 7 cases of retroperitoneal fibrosis with advanced gastric cancer. So, we report 7 cases of retroperitoneal fibrosis with clinical and radiologic characteristics. Results: The most common signs and symptoms were related irreversible renal dysfunction. The urologic finding noted medial deviation of the involved ureters and hydronephrosis Conclusions: Clinical manifestations are abdominal pain, back pain, hydronephrosis, and uremia. When sudden back pain and hydronephrosis develop in advanced malignancy, physicians should be considerd for retroperitoneal fibrosis.

인삼첨가 Long Life면의 조직감과 관능적 특성

정재홍,심창주,신영,권효진,이규희,오만진 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.5

인삼이 밀가루의 아밀로그래프에 의한 호화 성질과 파리노그래프에 의한 반죽 성질에 미치는 영향을 조사하였다. 인삼첨가 LL면의 조직감과 관능적 특성에 미치는 영향을 분석하기 위하여 밀가루에 대하여 인삼을 5.0∼10% 첨가하여 LL면을 제조한 뒤 면의 조직 특성 분석 및 조리시험을 하고 관능검사를 하였다. 인삼을 첨가하면 아밀로그래프의 호화개시 온도를 3.1℃ 지연시켰으며, 최고 점도를 40B.U 감소시켰다. 파리노그래프의 흡수율은 1.2% 증가하였으며, 반죽의 안정도는 인삼의 첨가로 2.0분 길게 측정되었다. Hunter L값은 인삼 첨가량이 증가할수록 낮아졌으며, Hunter a, b값은 반대로 측정되었다. 인삼첨가 LL면의 층밀림 압출력은 첨가량의 증가에 따라 높아져 10.0%의 첨가는 대조구 18.65(㎏f)보다 2.3(㎏f) 높은 23.95(㎏f)로 측정되었으며, 경도도 대조구보다 5.4(㎏f) 높게 측정되었다. 인삼첨가 LL면의 무게 증가는 인삼의 첨가량이 증가할 때 다소 높게 나타났으나. 부피 증가는 오히려 높게 나타나 조직이 다소 치밀한 것으로 나타났다 용출량은 인삼량의 증가에 따라 약간 감소하였다. 미생물은 인삼의 첨가량이 증가할수록 줄어들어서 장기 저장이 가능하였다. 관능 검사 결과는 인삼첨가 LL면이 4.2 및 4.0으로 비교적 좋은 점수를 얻었다. 이같이 LL면 제조시 인삼 첨가량은 7.0%의 수준이 효과가 컸다. The influence of ginseng on the paste or gelatinization properties by amylograph and mixing properties by farinograph of wheat flour, and on quality properties, color, cooking quality, textural and sensory properties, and reducing microbial population of LL (Long Life) noodles was studied. The contents of ginseng used were from 5% to 10% based on flour weight. The viscosity property of wheat flour with ginseng was increased the initial pasting temperature but the amylograph peak viscosity were decreased in vice versa. The farinograph absorption, stability and breakdown were increased by ginseng. The whiteness of LL noodles manufactured with ginseng was lower than that of control. The shear extrusion force and hardness of LL noodles manufactured with ginseng were shown much higher value than those of control. At cooking quality examination of LL noodles manufactured with ginseng, weight of cooked LL noodles was decreased but volume was appeared in vice versa. Extraction amounts of LL noodles manufactured with ginseng during cooking were much smaller than those of control. Total counts of microorganism of LL noodles manufactured with ginseng were decreased during storage at 30℃. Sensory properties of cooked LL noodles which was manufactured with ginseng showed quite acceptable. Based on the cooking and sensory evaluation test, addition of 7.0% ginseng to wheat flour may be suitable for processing LL noodles.

순천만 유입 수계의 수질 조사

허재선,심후진,전해숙 順天大學校 1999 論文集 Vol.18 No.1

Monthly survey on water quality of the branch streams of Sunchon Bay was carried out for the last 1 year (1998 to 1999) as a part of long term projects to conserve Sunchon Bay - a very important wetland supporting a well developed framework of biodiversity including several bird species threatened with extinction. Three points of Dongchon, main stram of Sunchon Bay, and five points of the branch streams - Seochon, Hyanglimchon, Okchon and Isachon were selected for the survey. The results of the water quality measurement are the followings; 1) According to the standard of river water quality amended by Korean ministry of Environment, the water quality of the branch streams of Sunchon Bay corresponded to the Second Level which is suitable for river water. 2) Monthly changes of the water quality were not variable, except that of September, 1998 during the survey. 3) Total nitrogen and phosphorus contents responsible for eutrophication were relatively lower than those measured in several rivers in Korea. However, careful regulation of nitrogen and phosphorus contents at the upper parts of Dongchon stream seems to be needed. 4) Isacnon, the largest branch stream of Sunchon Bay, was found to be less polluted and played a very important role in improving water quality by a dilution effect before the polluted and played a very important role in improving water quality by a dilution effect before the polluted water of Dongchon stream reaches Sunchon Bay. It was more likely that the water quality of Sunchon Bay was dependant on area source of pollutants emitted by agriculture and livestock farming rather than industrial effluents or residential wastewater.

Isoproterenol이 마우스 심근조직에 미치는 영향

최재화,김호덕,심태섭,라봉진 중앙대학교 의과대학 의과학연구소 1985 中央醫大誌 Vol.10 No.1

For the study, forty-six normal adult ICR mice were sacrificed. Extraction of the hearts was performed under ether anesthesia after two consecutive subcutaneous injections of isoproterenol hydrochloride (1-/3', 4'-dihydroxyphenyl/-2-isopropylaminoethanol HCI). The administered doses of isoproterenol to each group of the mice were 20mg, 35mg, 50mg, 80mg, 100mg, 150mg, and 200mg per kilogram of body weight respectively, After extraction, the wet weight of each of the hearts was measured. Five μm-thick paraffin sections of the heart tissue fixed with alcohol-formalin were stained with hematoxylin and eosin for routine study, with van Gieson's method for collagen, and with von Kossa's method for histochemical study of calcium salt. Stained sections were examined under light microscope. The results were as follows: 1. All of the mice treated with isoproterenol showed myocardial necrosis-like lesions. These lesions spread from subendocardial area to entire myocardium in accordance with the increase of dose of isoproterenol and the prevalence rate of myocardial necrosis by isoproterenol administration was higher in the left ventricle than in the right ventricle. 2. The most typical histopathologic findings of myocardial necrosis were observed in the mice treated with 80mg of isoproterenol per kilogram of body weight. 3. The mice treated with 100mg, 150mg, and 200mg of isoproterenol per kilogram of body weight showed the death rate of 50%, 83%, and 100% respectively after the first administration. 4. All of the mice treated with isoproterenol showed calcium deposits in the myocardium. 5. The ratio of the wet weight of heart to the body weight increased in the mice treated with isoproterenol in comparison with the control mice.