생약추출물 유도형 Nitric Oxide Synthase 저해활성 검색

류재하,이소영,박재현,이화진,장세란,은진희,김남이,정연수,장미경,최영은,이숙현,손행자,안한나,고혜진 숙명여자대학교 약학연구소 2001 약학논문집-숙명여자대학교 Vol.17 No.-

Nitric Oxide (NO), derived from L-arginine, is produced by two types (constitutive and inducible) of nitric oxide synthase (NOS: cNOS and iNOS). The NO produced in large amounts by the iNOS is known to be responsible for the vasodilation and hypotension observed in septic shock and inflammation. The inhibitors of iNOS, thus, may be useful candidate for the treatment of inflammatory diseases accompanied by the overproduction of NO. We prepared alcoholic extracts of herbal drugs which have been used for the treatment of inflammation in oriental medicine. We have screened the inhibitory activity of NO production in lipopolysaccharide (LPS)-activated macrophages after the treatment of these extracts. Among the 81 kinds of extracts of herbal drugs, 34 extracts showed potent inhibitory activity of NO production above 50% at the concentration of 50 (μg/ml. The inhibitory activities of NO production were also evaluated for several solvent fractions at three different concentrations. Especially, hexane soluble fractions of Agrimonia pilosa, Hydrangea serrata, Machilus thunbergii, Prunella vulgaris, Saussurea lappa, Tussilago farfara, and ethyl acetate soluble fractions of Angelica gigas, Ostericum koreanum, Torilis japonica, Perilla frutescence showed moderate activity at 10 and/ or 25 (μg/ml. These fractions are promising candidates for the study of the activity-guided chromatographic purification of active compounds.

일부 소규모 시계조립 사업장의 상지 누적외상성장애의 유병률

장은철,김현주,권영준,박시복,이수진,송재철 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.4

목적 : 단순 반복작업을 하는 시계조립 사업장 근로자를 대상으로 상지 누적외상성장애의 자각증상 유병률과 상지 누적외상성장애 유병률을 파악하고자 하였다. 방 법 : 인구학적 특성, 상지의 자각증상, 사회심리적 요인은 자가 설문지법으로, 인간공학적 특성은 점검표를 이용한 면담법으로 조사하였다. 상지의 자각증상 조사와 사회심리적 요인은 각각, N10SH (1993) 설문지와 Karasek(1979)의 Job strain model을 번역하여 조사하였다. 상지 누적외상성장애 판정은 의사가 작업 현장에서 직접 환자 면담과 이학적 검사를 수행한 후, 연구에서 규정한 증례정의에 의해 수행하였다. 수집된 자료는 x2 teat와 exact test로 단별량 분석을 하였고, 이중 의미있는 변수와 위험 인자로 알려진 변수들로 다중 로지스틱 회귀분석을 시행하였다. 결 과 : 대상자들의 자각증상 유병률은 54.2 %였고, 부위별로는 목 34.9 %, 손/손목 31.3 %, 어깨 30.1 %, 팔/팔꿈치 18.0 %순이었다. 상지 누적외상성장애의 유병률은 45.8 %였고, 근막동통증후군이 26명으로 가장 많았고, De Quervain씨 병 8명, 수부/전완부의 건염 및 건활막영 8명, 수근관 증후군 5명, 이두건염 5명, 척골 신경병증 5명, 내상과 염 4명, 외상과염 2명, 경추 디스크 질환 2명, 흥곽 출구 증후군 1명순으로 나타났다. 단변량 분석시 자각증상 유병률은 작업부서 중 완성 부에서, 상지 누적외상성장애 유병률은 여자와 완성 부에서 통계학적으로 유의하게 높았다. 다중 로지스틱 회귀분석 결과 완성부와 근무긴장도가 높은 군이 상지 누적외상 성장애와 통계학적으로 유의한 결과를 보였다. 결 론 : 이 연구에서 상지 누적외상성장애 유병률은 45.8 %으로서 저강도의 단순 반복작업을 하는 시계조립 사업장 근로자에서도 누적외상성장애의 위험이 있는 것으로 생각된다. 또한, 사회심리적 요인과 작업 부서별 공정 및 환경이 유병률에 영향을 미치는 주요 요인으로 생각되며, 향후 이들에 관한 다양한 연구가 진행되어야 할 것이다. 산업보건 현장에서는 시계조립 사업장과 같이 저 강도의 단순 반복작업을 위주로 하는 사업장에 대한 지속적인 관심과 각 사업장별 위험요인을 파악하여 적절한 예방 및 관리 대책을 세워야 할 것이다. Objectives : The prevalence of cumulative trauma disorders of upper extremities mons watch assembly workers in small-scaled industry was studied. Methods. In 83 workers at five watch assembly factories, symptoms and psychosocial questionnaire, ergonomic Interview, physical examination were conducted. Results : Prevalence of self-reported symptoms was 54.2% and neck 34.9%, wrist/hand 31.3%, shoulder 30.1%, elbow/arm 18.0% by anatomical site. Prevalence of cumulative trauma disorders was 45.8%. Most common disease was myofascial pain syndrome (31.3%). The other diseases were De Quervain disease (9.6%), tenosynovitis/tendinitis at wrist/hand (9.6%), bicipital tendinitis(6.0%), fat. epicondylitis(4.8%), meIn. epicondylitis(2.4%), and cervical disc disease(2.4%). As result of ergonomic interview, repetitiveness was 79(98.8%). There were no differences in the prevalence of self-reported symptoms and cumulative trauma disorders for age, sex, marital status, duration of work In the study, The significant factors of cumulative trauma disorders were occupational task and psychosocial stress in the study. The prevalence of cumulative trauma disorders in high strain group was hlgher (68.4%) than other three groups. The prevalence of self-reported symptoms and cumulative trauma disorders in assembly task was higher (70.0%, 64.0%) than other two tasks. Conclusions : Prevalence of self-reported symptoms was 54.2%, prevalence of cumulative trauma disorders was 45.8% in watch assembly workers. In this study, factor related to self-reported symptoms was occupational task and factors relaxed to cumulative trauma disorders were occupational task and psychosocial stress. This results suggest that differences of ergonomics and environment in occupational task cause differences of prevalence of self-reported symptoms and cumulatlve trauma disorders.

韓國人의 各 臟器組織中 靑酸鹽의 微量分布에 關한 硏究(Ⅱ)

張成吉,金恩浩,金英雲,李鎭勳,柳在薰,權五星,金明德,吳世珍,金由那 內務部 國立科學搜査硏究所 2001 國立 科學 搜査 硏究所 年報 Vol.33 No.-

3차원 적층패키지의 Cu-Cu 접합부의 정량적 접합강도 평가 및 향상에 관한 연구

장은정,김재원,Kim, Bioh,Thorsten Matthias,현승민,이학주,박영배 한국공작기계학회 2009 한국공작기계학회 춘계학술대회논문집 Vol.2009 No.-

The quantitative interfacial adhesion energy of Cu-Cu direct thermo-compression specimen with bonding temperature and wet pre-treatment was measured by using 4 point bending test for 3-D IC integration. The evaluated interfacial adhesion energy was 2.79±0.82, 3.46±0.87, 4.93±0.2 J/㎡ bonding temperature 300, 350, 400℃ and 0.29, 1.28, 1.64, 1.17 and 0.43 J/㎡ acetic acid pre-treatment at 35℃ for 0, 1, 5, 10, and 15 min. From the result, 5 min treated Cu to Cu thermo-compression bonding has the highest interfacial adhesion energy at 350℃.

최근 5년간 급성 A형 간염으로 입원한 환자들에 대한 입원 내용 분석

장재영,유병욱,조용진,오정은,홍성호,조주연 순천향대학교 의학연구소 2009 Journal of Soonchunhyang Medical Science Vol.15 No.1

Abstract Background : Due to improvements in Korea's sanitary conditions, antibody rates to the hepatitis A virus is decreasing rapidly and admission due to the disease is increasing, Patients who were admitted for hepatitis A during the last 5 years were selected and yearly differences in admission, clinical picture, medical fees, and differences according to age and gender were analyzed. Methods : Patients who were admitted to the Seoul Soonchunhyang University Hospital during the 5 year period between May of 2003 and April of 2008 and tested positive for IgM anti-HAV were selected and their medical records retrospectively analyzed. Results : 156 subjects [91 male (58%), 65 female (42%) were selected. The average age was 29.2 years (range; 21.4-37), average admission period 12.6 days (range; 12.5-1 7.7) days, and average medical fees 2,295,151 (range; 932,097-3,658,205) KRW(Korean Won), Yearly incidence rates were as follows : 10 for May, 2003 to April, 2004, 14 for May, 2004 to April, 2005, 23 for May, 2005 to April, 2006, 69 for May, 2006 to April, 2007, and 41 for May, 2007 to April, 2008, The maximum value median of AST, ALT, and bilirubin during admission was 989 IU/L, 1999 IU/L, and 5.6 mg/dL each, Each factors were all positively correlated with the patient's age. Conclusions : Recent decrease in antibody positivity of the hepatitis A virus and subsequent surge of acute hepatitis A is a cause of rising socioeconomic costs. A large scale epidemiologic research on whether HAV vaccination should be made mandatory and health insurance applied seems to be needed. Key words : Hepatitis A, Age, Medical fees, Vaccination

카이로프랙틱 도수요법에 의한 경추성 두통의 치료

장재호,사공은성 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.4

Purpose : Cervicogenic headache is a neck induced headache syndrome. Chiropractic Spinal Manipulation Therapy (CSMT) is non-invasive treatment method for painful lesion. CSMT is considered to relief the pain and increase the range of motion of cervical spine without complications. Materials and Methods : A follow-up observation has been made by retrospective study after applying chiropractic spinal manipulation therapy on 28 cases of cervicogenic headache patients who visited our hospital from April 2005 to April 2007. Every patiens had performed Short-Form McGill Pain Questionnaire, Neck Disability Index, and SF-36 Questionnaire. Results : 28 patients average age was 37(14∼73). Female patient was 23 out of 28 and 5 was male patients. The average duration of treatment was 13.6 weeks. The Short-Form McGill Pain Questionnaire, in which questions about the level of pain in general and the Pain Relating Index (PRI), was 11.7 in average before chiropractic spinal manipulation and 3.7 in average after manipulation (P=0.196). The Visual Analog Scale (VAS 0-10) showed pain decrement from 6.1 to 3.1 after manipulation (P=0.126). PPI showed improvement from 2.75 to 0.5 after manipulation(P=0.1). In the Neck Disability Index, which questions the physical disability due to pain, the decrement in average was 14.6 to 4 after manipulation. The results of SF-36 Questionnaire, which deals with the patient's general health showed decrement from 57.8. to 69.4(P=0.317) after manipulation. Conclusion : CPMT has pain relieving effects in cervicogenic headache and also shows increased neck range of motion after treatment. Therefore, CSMT can be a one of useful treatment method for cervicogenic headache.

Wigner 분포 함수를 이용한 위상지연 함수에 대한 연구

장남영,은재정,강호정,최평석 國立 昌原大學校 産業技術硏究所 2000 産技硏論文集 Vol.14 No.-

작은 초점 심도를 갖는 원형 대칭 광시스템에 대하여 Wigner 분포 함수(WDF)를 적용해 얻은 위상 지연 함수와 초점 심도와의 관계를 설명한다. 일반적인 광시스템의 이차원의 원형 개구는 잘알려진 변환식에 의해 일차원 동공 함수로 변환되고 변환된 일차원 동공 함수는 다시 Wigner 함수로 표현된다. 일반적으로 일차원 함수를 Wigner 함수로 변환시키면 2차원 함수가 된다. 초점 심도와 위상 지연 함수와의 관계는 2차원 위상 공간(χ, ζ)으로 표현되는 Wigner 함수를 χ축으로 α만큼 기울이므로써 얻을 수 있다. 여기서 초점 거리 f를 1220mm, 구경이 12.5mm, 사용된 광파장 λ가 633nm일 때 α값에 따른 초점 심도의 변화를 수치해석 한다.

관상동맥경화증 환자에서 전곡섭취가 지질과산화 및 혈중 인슐린과 호모씨스테인 농도에 미치는 영향

장양수,이종호,엄영람,조은영,바현영,황재관,여익현 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.6

메이크업 시 사용되는 퍼프의 사용기간에 따른 오염도에 관한 연구

류재기 ; 김은주 ; 장철수 김천대학교 2010 김천대학교 논문집 Vol.31 No.-

The purposes of this study are to investigate the level of bacteriological contamination of puffs which are used most frequently for touch ups during the day as a makeup tool, and to compare the degree of contamination before and after washing puffs with a neutral detergent. A total of 40 powder puffs that were used for six months to three years have been collected since May, 2005. The collected puff samples were cut into the size of lem by a sterilized scissor, and were put into a sterilized test tube that contained 5ml of DW (distilled water). Then the tube was strongly agitated. Supernatants (100ml) con-taining bacteria was applied to TSA (Trypticase Soy Agar), MacConkey agar, and SDA (Sabouraud Dextrose Agar) plates, respectively. The TSA (Trypticase Soy Agar) and MacConkey agar plates were incubated at 37C for 24-48 hours. The SDA (Sabouraud Dextrose Agar) plates were incubated at 28 C for 7-14 days. As a result of the level of contamination of puffs used over a certain period of time, there was no significant difference cxcept aerobic bacteria. Staphylococcus was not detected. Aerobic bacteria was detected from the puffs older than one year and the colony count increased sharply. The puffs used for more than two years had three times more colony count than the one-year old puffs. In particular, out of 10 puffs older than two years, two were found with 500CFU/ml exceeding the limit of microbiology of cos-metics. Also, the puffs older than two years had 31,1% less microbiology after washing with a PH neutral detergent than before washing. Therefore, as the period of using puffs is longer, thorough checks of their hygiene are needed; as the number of using times increase or the length of using times is longer, frequent washing is inevitable. Since this study restrained the number of puffs to 40,7 would be problematic for generalization and a secondary experiment would be necessary for precise identification of cultured germs.

25㎜ NSW-ring, pp.를 充塡한 充塡塔에서 암모니아 吸收에 의한 充塡物의 特性硏究

申恩才,朴出在,金彰昊 동아대학교 환경문제연구소 1995 硏究報告 Vol.18 No.1

This study was carried out to demonstrate the superiority of used packing in view of energy saving and efficiency of mass transfer, comparing with conventional packing. The results are as follows : 1. Owing to low pressure drop under high load, 25mm NSW-ring, pp. can cause energy saving. 2. The unique magnitudes of used packing are as follows : C_(G) = 5.78 m = 0.67 n = 0.46 3. Used packing can make high efficiency including energy saving because of low pressure drop per the number of transfer unit. To rate the characteristic of packing, it should be carried out that the measurement of pressure drop per packing height and per the number of transfer unit. This study demonstrated the superiority of used packing by caning out above experiment and could be used as basic reference for design and predicting efficiency of packing tower which is filled with same packing.