Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

트레드밀 운동시 흡기근 테이핑이 폐활량에 미치는 영향

김민지,신수영,송월섭,조수진,최동락,황미진,황진규,박진현,김경,Dennis W. Fell 대구대학교 특수교육재활과학연구소 2011 再活科學硏究 Vol.29 No.1

이 연구는 트레드밀 운동과 키네시오 테이핑의 효과에 따른 폐활량의 변화를 알아보기 위해 26명의 비흡연자가 참가하였으며 키네시오 테이핑을 적용하지 않은 그룹과 키네시오 테이핑을 적용한 두 그룹으로 무작위로 배정하고 각 군들을 주 3회 6주간의 트레드밀 훈련을 실시하였다. 실험 전과 후, 스파이로미터를 사용하여 키네시오 테이핑의 적용 따른 폐활량 변화 효과를 측정하였다. 이 연구의 결과를 종합해보면 키네시오 테이핑을 적용 그룹에서 적용하지 않은 그룹에 비해 FVC, FEV1에서 유의한 증가를 보였다. 이러한 결과는 건강한 성인에서 키네시오 테이핑을 적용한 경우 키네시오 테이핑을 적용하지 않고 트레드밀 운동을 한 경우보다 폐활량의 향상에 효과적이라고 생각되어진다. The purpose of this study was to investigate the variation of vital capacity(VC) according to the effects of kinesio taping with treadmill exercise. Twenty-six non-smokers were participated in this research and these subjects were randomly assigned into two groups. To measure the VC variation effects of kinesio taping, spirometer was used. The collected data were analyzed statistically by using a paired Mauchly test and repeated measure ANOVA. The results of this study were as follows; A group: treadmill with kinesio taping B group: treadmill without kinesio taping. Between A and B, there were significant differences. In the case of A group, there was 11.66% increase of VC, during 3 weeks experiment(p<.01). In the case of B group, there were significant differences, 3.35% increase of VC, during 3 weeks taping intervention(p<.01). After 6 weeks experiment, the improvement of VC shown a significant difference with intergroup (p<.05). From this result, it was revealed that treadmill exercise with kinesio taping was effective to improve VC to healthy adult than treadmill exercise without kinesio taping.

개인식별을 위한 컴퓨터 영상 중첩시스템 연구

송현교,김하진,강민구 호남대학교 정보통신연구소 1996 정보통신연구 Vol.5 No.-

임포즈란 신원 미상의 두개골을 발견했을 때, 두개골의 사진과 동일인으로 판정되는 사람의 생전 사진을 서로 같은 비율로 축소한 후 두 사진을 겹침으로써 두 인물간의 동일인 여부를 비교, 검토하는 과정을 말한다. 이에 본 논문에서는 국립과학수사연구소에서 제 검사 및 DNA 유전자 검사를 통해 이미 동일인임이 확인된 7명의 두개골 사진, 생전사진을 이용하여 컴퓨터 프로그램을 이용한 응용프로그램을 개발하였다. 신원 미상의 두개골을 비디오 카메라, 컴퓨터를 이용, 영상처리후 임포즈하고 해부학적 특징으 기준으로 동일인여부를 비교, 검토하는 과정에서 다음과 같은 결과를 얻었다. 1. 두개골 및 생전사진을 처리하는 방법으로 다양한 영상처리 기법(Smooth, Sharpen, Emboss, Mosaic, Engrave, Invert, Neon, Color to Mono)을 개발하였고, 이들 중 Neon, Invert, Engrave기법에서 최적의 영상을 얻을수 있었다. 2. 다양한 방식(SrcOr. SrcAnd. SrcInvert. SrcErase,DstInvert. MergePaint) 으로 두 영상을 합성하여 임포즈 감정시 필요한 최적의 비교상태를 얻을수 있었다. 3. 비디오 카메라를 이용하여 두개골상을 직접 컴퓨터에 입력할 수 있었으며, 이를 생전사진과 임포즈함으로써 보다 정확하고 신속한 감정을 가능 하게 했다. 이상에서 볼 때 기존의 수작업으로 이루어졌던 임포즈가 이제는 컴퓨터 프로그램에 의해 이루어지므로서 보다 신속하고 정확한 임포즈 작업을 가능하게 할 것이라 사료된다. A Study on the Computer Impose System for Individual Recognization *Dept. of inform. and Comm Eng.. Honam University ** The Dental School of Yonsei University In this thesis. a new superimposition scheme using a computer vision system was proposed with 7 pairs of skull and ante-mortem photographs. which were already identified through other tests and DNA fingerprints at the Korea National Institute of scientific Investigation, At this computer vision system. an unidentified skull was caughty by video-camcoder with the MPEG and a ante-mortem photograhp was scanned by scanner. These two images were processed and superimposed using pixel processing. Recognition of the individual identification by anatomical references was performed on the two superimposed images. This image processing techniques for the superimposition of skull and ante-mortem photographs simplify used the previous approach taking skull photographs and developing it to the same size as the ante-mortem photographs. This system using various image processing techniques on computer screen. a more precise and time-saving superimposition technique could be able to be applied in the area of computer individual indentification.

貝類의 冷凍에 關한 硏究 : 4. 오분자기의 凍結貯藏溫度에 따른 品質의 變化 4. Quality Changes of Abalone, Haliotis diversicolor japonica, by the Freezing Storage Temperatare

宋大鎭,河璡桓,金洙賢 濟州大學校 海洋資源硏究所 1985 해양과환경연구소 연구논문집 Vol.9 No.-

오분자기를 보다 效果的으로 利用하기 위한 基礎資料를 얻기 위하여 오분자기를 脫殼, 凍結한 후 凍結貯藏中의 品質의 變化를 實驗하였다. 凍結貯藏中 pH는 거의 變化가 없었고 drip量 조금씩 增加하였으며 揮發性鹽基窒素도 조금씩 增加하였으나 -35℃의 경우 3個月까지는 좋은 鮮度를 維持하였다. 保水力은 점차 減少하여 貯藏 3個月후에는 -10℃에서 貯藏한 경우가 10%, -35℃는 약 7%까지 減少하였다. hardness는 -10℃와 -35℃ 다 같이 1個月까지 增加하다가 以後 급격하게 減少하였으며 toughness는 계속 減少하였다. cohesiveness는 -10℃의 경우 큰 變化가 없었으나 -35℃의 경우는 2個月에 급격히 減少하였으며 elasticity는 -10℃와 -35℃의 경우 모두 큰 變化가 없었다. 色調의 變化에서 a값은 큰 變化가 없었으나 L값은 계속 減少하였고 b값과 ΔE값은 계속 增加하였다. 組織學的 觀察의 結果 貯藏溫度가 높은 것일수록 氷結晶의 成長과 근속끼리의 응집이 현저함이 確認되어졌다. To obtain the basic data for utilizing abalone, Haliotis diversicolor japonica more effectively the quality changes as pH, drip, water holding capacity (WHC), texture, color difference and histological observation in shelled and frozen products during storage were carried out. It was noticed that the pH of frozen products (-10℃ and -35℃) were not changed markedly, thaw exudate (drip) and volatile basic nitrogen (VBN) were slightly increased as the storing period prolonged but fairly good freshness was maintained until 3 months in the storage of -35℃. The water holding capacity for all frozen products were steadily decreased to show 10% and 7% down at -10℃ and -35℃ respectively with 3 months storage on the base of raw materials. With all frozen products it was observed that hardness was increased up until the 1 month storage period and then suddenly dropped while toughness was decreased steadily. NO significant changes in cohesiveness with the -10℃ storage were observed but with -35℃, it was dropped rapidly after 2 months storage while elasticity was not changed markedly for all frozen products. The color difference expressed as a value was not changed markedly while the L value was decreased steadily. The b and ΔE value were increased continually as the frozen period was extended. It was confirmed that both the growth of ice crystal and aggregates of muscle bundle were prominent at the higher storage temperature in histological observation.

저염농도의 자리발효식품의 가공에 관한 연구

송대진,김재하,강영주,김수현,고영환,하진환 濟州大學校 工科大學 産業技術硏究所 1990 産業技術硏究報告 Vol.1 No.-

This study was carried out to obtain the basic data on salted and fermented damsel fish(Jari-Jeot), one of the important traditional marine foods in Cheju-Do. and also to seek salt lowering method for the development of a new local tourist food. The results are as follows : The best organoleptic results were obtained after 60 days' fermentation for 25.0, 12.5 and 10% of salt-added group, and around 45 days for 7.5 and 5% of salt-added group. In raw fish ingredient IMP was abundant which marked 17.7 μmole/g while in fermented fish hypoxanthine was predominant but ATP and ADP were not detected after 30 days' fermentation. Sixteen kinds of free amino acids were detected and identified in Jari-Jeot instead of 17 kinds of those in raw sample. The abundant amino acids in Jari-Jeot after 60 days' fermentation were lysine. alanine, glutamic acid, glycine and leucine, and those were consisted of approximately 60% of the total free amino acids. During fermentation the content of TMAO was decreased gradually and no TMAO was detected after 75 days' fermentation while that of TMA was increased during fermentation up to 45 days' and decreased afterwards. The fatty acid of low-salt Jari-Jeot was composed of 31.9% of saturated acids, 13.2% of monoenoic acids and 54. 9% of of polyenoic acids. While the content of EPA in polyenoic acids was 1.9%, that of DHA was 23.0%. In order to lower salt concentaration of fermented damsel fish, additon of Pediococcus halophilus culture as a starter together with 10-12.596 of salt, 4-5% of KCI, and 2% of glucose gave as good quality as traditionally made Jari-Jeot.

흉부 둔상 후 8년간 지속된 삼첨판폐쇄부전의 치료 1예

송현주,남승현,최영주,박성훈,박시훈,한재진 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.4

전공의들의 직업적 유해인자 및 직업만족도

송혜란,김진하,변주현,이광영,이덕희,김호찬 大韓産業醫學會 2003 대한직업환경의학회지 Vol.15 No.3

목적: 전공의는 여러 유해인자에 노출되어지고 있으며 과중한 업무로 인한 수면 부족에 시달리고 있다. 본 연구는 전공의의 건강상태의 기초 자료를 제공하고 향후개선 방안을 도출하기 위하여 시행하였다. 방법: 연구대상은 2000년 3사관학교와 국군군의학교에 입소한 전체 1,316명 중 전공의 수련과정 없이 입대한 의사와 치과의사, 한의사를 제외한 881명을 대상으로 설문지를 배포하여 784부(회수율: 89.0%)를 회수하고 이중 분석이 가능한 704(79.9%)명의 자료를 최종 분석하였다. 결과: 월평균 당진횟수는 레지던트 1년차에서 19회 정도이었다. 당직 시 평균 수면시간은 레지던트 1년차에서 4시간미만 이었다. 수련기간 동안 전공의의 20%이상에서 방사선, 레이저, 살균소독제, 마취가스, 항암제에 노출되었으며 예방대책은 방사선과 레이저의 노출의 경우 35%정도를 제외하고는 10%내외로 조사되었다. 연구대상자중 91.1%에서 수련기간 동안 주사침이나 시술에 의해 손상을 입었으며 그 중 본인이 감염된 경우는 HBV 2.3%(4명), HCV 0.6%(1명)으로 조사되었다. 응답자의 28.4%에서 자신의 직업에 불만족 하였으며 96.5%가 과중한 업무부담을 느꼈고 69.5%가 동일한 임금수준에서 직업을 바꾸고자 한다고 응답하였다. 결론: 전공의는 수련기간 동안 물리적, 화학적, 생물학적 유해인자에 노출되고 있었으나 이에 대한 보호나 예방대책은 거의이루어지지 않고 있었으며 과중한 업무부담으로 수면부족을 초래하여 가장 중요한 환자 진료에 지장을 받고 있었다. 수련기간 동안 대다수가 의사로서 직업에 대한 흥미를 가지고 있었으나 업무부담과 진료이외의 업무 등으로 소득과 조건이 비슷하다면 다른 직업을 선택하고자 하였다. 이상의 결과를 토대로 전공의들을 위한 유해인자의 보호대책 및 예방대책을 마련하며 과중예방대책이 마련되어야 하고 업무환경개선 및 업무로 인한 건강장해에 대한 정당한 보상이 향후에 마련되어야 할 것이다. Objects: Residents are exposed to various hazards and harassed by sleep dificiency due to overwork. This study was performed to outline the health condition of residents and develop the ways of improving it. Mehtods: A questionnaire was distributed to 881 army doctors who enrolled at the Korea Third Military Academy and The Armed force Health Service School in 2000 with the exception of medical doctors who joined the armed service without going through intern and resident periods, dental and oriental medical doctors. 784(89.0%) of the 881 questionnaires were resident of which 704 cases (79.9%). This should actually be 89.0% were regarded for analyses as being reliable data. Results: More than 20% of residents were exposed to radiation, laser, disinfectants, anesthetic gas, and anticancer drugs during their training periods with preventive neasures for each of these hazards taken in about 10% of the cases of the lesser hazards but in about 35% for the hazards from radiation and laser. 91.1% of residents had experience of needlestick injuries during theirtraining periods with over 36.3% being exposed to patients with infection during that time. The cases contracting HBV and HCV diseases due to pricking are 2.3% and 0.6% respectively. The average number of night duties per month during a one-year resident's period was about 10. The average sleeping time on duty during a one-year residents was less than 4 hours. 28.4% of respondents were not satisfied with their occupation, 96.5% delt tired from overwork and 69.5% wanted to change their occupation to another that would pay the same salary. Conclusion: Residents are exposed to harmful physical, chemical and biological factors, but the preventive care seldom undertaken. In addition, their overwork brings about lack of sleep which causes them problems when giving medical treatment to patients during their training periods. Despite the resident's interest in an occupation as a doctor, many intend to select other occupations if they can receive the same salary. According to the above results, preventative systems and measures should be prepared with environmental improvements for residents, and proper rewards for their overwork should be accomplished

1994∼1995년 한국에서 발생한 가을철 열성질환에 대한 혈청역학적 연구

송기준,송진원,백락주,김상현,김세연,이용주 대한감염학회 1998 감염 Vol.30 No.4

목 적 : 최근 우리나라에서 발생했던 금성 열성질환의 전반적인 혈청역학적 양상의 분석을 위해 1994년부터 1995년까지 고려대학교 의과대학 미생물학교실, 바이러스병연구소에 급성 열성질환으로 검사가 의뢰된 환자혈청 5330건을 쯔쯔가무시병, 발진열, 신증후출혈열, 라임병 및 랩토스피라증에 대한 항체를 조사하였다. 방 법 : 1994년부터 1995년까지 2년간 고려대학교 의과대학 미생물학교실, 바이러스병 연구소에 검사가 의뢰된 환자혈청을 주 대상으로 간접면역형광항체법 검사로 쯔쯔가무시병, 발진열, 신증후출혈열 및 라임병에 대한 항체양성 유무를 검색하고 macroscopic agglutination법으로 렙토스피라증에 대한 항체를 조사하였다. 결 과 : 열성질환 환자 혈청 5330건을 대상으로 신증후출혈열, 쯔쯔가무시병, 발진열 및 라임병에 대한 항체검사 결과 쯔쯔가무시 항체양성자가 12% (640/5330)로 항체양성자 중 여자가 60%로 60대가 30% (192명)로 가장 많았다. 월별 분포에서는 대부분의 양성자 (89%)가 가을철에 집중되어 있었다. Richettsia, typhi에 대한 항체를 양성률을 보였다. 발진열 항체 양성자 중 남자가 60% (106/178)로 여자보다 많았으며, 모든 연령 층에서 발견되었으나 40∼50대가 57% (107명)로 가장 많았다. 대부분이 양성자 (44%)가 늦가을 (10∼12월)에 발생하였다. Macroscopic agglutination법에 의한 렙토스피라에 대한 항체률을 조사한 결과 1% (55/5330)의 항체 양성률을 보였으며 연도별로는 1994년 0.4% (11/2799), 1995년 1.7% (44/2531)로 1995년에 더 많은 항체 양성자가 발생하였다. 총 55건의 렙토스피라 항체양성자 중 남자가 71% 였으며, 연령별 분포에서는 60대가 47%로 가장 많았으며 20대 이상의 성인에서만 항체가 발견되었다. 대부분의 항체양성자 (78%)가 8∼10월에 집중되었다. 라임병은 총 5330건 중 항체양성자는 없었다. 결 론 : 1994∼1995년 우리나라에서 발생한 발열성질환환자 중 쯔쯔가무시병, 발진열, 신증후출혈열 및 렙토스피라증 환자가 전체의 29%였으며 항체 양성자수로는 신증후출혈열,쯔쯔가무시병,발진열,렙토스피라증 순이였다. 따라서 위의 4가지 질환이 우리나라에서 특히 가을철에 발생하는 급성열성질환의 주원이으로 생각되나 새로운 질병이나 신종 병원체에 의한 동일한 질환이 존재할 가능성이 매우 높으므로 지속적인 혈청역학적 조사가 수행되어야 할 것이다. Background :The principal acute febrile diseases reported in autumn, Korea, have been hemorrhagic fever with renal syndrome (HFRS), scrub typhus, murine typhus and leptospirosis. Methods : To evaluat the seroepidemiologic patterns of acute febrile illness in Korea, sera were collected from 5330 patients from 1994 to 1995, and examined for antibodies against Hantaan virus, Orientia tsutsugamushi, Rickettsia typhi, and Borrelia burgdorferi by indirect immunofluorescent antibidy technique (IFA) and by macroscopic agglutination test for Leptospira interogans. Results : Of 640 seropositive cases against O. tsutsugamushi, 60% were female; age group of the seventh decade occupied 30%; 89% were reported during October and November. By IFA test, the seropositive rate of murine typhus was 3.5% (18/5330) with high incidence rate (44%) in the period from October to December, and males in their forties and fifties were mostly affected. Cases seropositive for leptospirosis consisted 1% of 5330 acute febrile illness cases. Of 55 seropositive cases, 71% were male; 71% were in their sixties; 78% occurred during the period from August to October to January and males in the thirties were mostly infected. No seropositive case of Lyme disease was present. Conclusion : The above results indicate that hantavirus, O. tsutsugamushi, R. typhi, and L. interogans were the main causative agents of acute febrile diseases during autumn season in Korea.

유방암에서 종양의 크기와 액와림프절 전이의 상관성을 근거로 한 액와림프절 절제술의 생략

송근영,조현진,김권천 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Purpose: The aim of this study is evaluation of the relations between axillary lymph node status and tumor size, and then applied the omission criteria of axillary lymph node dissection in early breast cancer. Materials and Methods: We reviewed 110 patients underwent radical mastectomy and axillary lymph node dissection in breast cancer at Chosun university Hospital between Feb. 1996 and Mar. 2003. We evaluated the correlations between clinicopathologic factors, axillary lymph node status, and presence of metastasis. Results: Correlations between clinicopathologic factors and axillary lymph node status were statistically significant (p=0.01). Correlation between locoregional metastasis and distant metastasis and axillary lymph node status was significant (p=0.03). Results from the number of histologically-positive lymph nodes were significant (p=0.02) as that of lymph node status. Conclusions: The rate of axillary lymph node metastasis in Tla breast cancer is minute. And, preoperative evaluations and postoperative routine radiation therapy can control the axillary lymph node metastasis in Tla breast cancer. So, omission of the axillary lymph node dissection in Tla breast cancer may be considerable.

스케일이 있는 급수관내의 유도초음파의 전파 특성에 관한 연구

송성진,김영환,이동훈,이현동,배철호,박정훈 한국비파괴검사학회 2003 한국비파괴검사학회지 Vol.23 No.1

배관 내의 스케일은 통수비를 감소시키므로 배관을 적절히 관리하기 위해서는 스케일량에 대한 정량적인 평가가 필수적이다. 상수도 급수관내의 스케일량을 평가하기 위해서 유도초음파를 사용하였다. 가변각 웻지를 사용하여 유도 초음파의 여러 모드를 발생시키고, 노후 배관을 따라서 전파하는 특성을 분석하였다. 실험적으로 관내의 스케일이 증가함에 따라서 f×d=1.5㎒·㎜인 경우에 F(M,2) 모드들의 진폭이 크게 감소하는 것을 알 수 있었다. 본 연구의 결과로 상수도 급수관의 스케일량 평가에 적합한 유도 초음파는 F(M,2) 모드들임을 알 수 있었다. Since the scale in pipes reduces the flow rate, a quantitative evaluation of the scale is essential for the proper maintenance of pipes. Guided waves were employed to estimate the amount of scale in water supplying pipes. Using variable angle wedge, several modes of guided waves were generated and their propagation charcteristics along the pipes with scale were analyzed. It was experimentally observed that the amplitude of F(M,2) modes at f×d=1.5㎒·㎜ decreased significantly with increasing amount of scale. The present study showed that F(M,2) modes were optimal to evaluate the scale in water supplying pipes.