Encephalomyocarditis Virus 표면항원의 단일항체 생산세포주의 크론과 이의 면역학 및 생화학적 연구(Ⅱ)

양종대,박종수,이영탁,김화영,김영래,이인수,조영준,박재윤,차종희,윤지원,고광삼 朝鮮大學校 附設 醫學硏究所 1987 The Medical Journal of Chosun University Vol.12 No.1

To see whether there is any differencies in RNA dependent DNA polymerase activities between monoclonal antibody-producing hydridoma cells and non-producing hybridoma cells, Balb/c female mice were immunized with the purified viral surface protein of D-variant of encephalomyocarditis virus and then fused with myeloma cells (NR-1). After cloring, monoclonal antibody-producing hybridoma cell lines were separated from non-producing hydridoma cell lines. RNA-dependent DNA polymerase activities were measured in the supernatant of monoclonal antibody-producing hybridoma clones and non-producing hybr idoma clones, and myeloma cells as control, Monoclonal antibody-producing hybridoma cells showed statistically significant higher activity as compar compare to that of nonproducing hybridoma cells. To find whether RNA-dependent DNA polymerase releasing cells aware secreting or budding C-type virus particles, those cells were examined with electron microscope. The hybridoma cell which secrete large amount of RNA-dependent DNA polymerase shows significant number of extracellular C-type virus particles. In constrast, non-producing hydridoma cells contains a lot of intracellular C-type virus particles. It is concluded that monoclonal antibody-producing hydridoma cells released particles. It is concluded that monoclonal antibody-producing hydridoma cells released significant amount of RNA-dependent DNA polymerase land extracellular C-type virus particles, while non-producing hydridoma cells showed less release of RNA-dependent DNA polymerase and contains intracellular C-type virus particles.

가족공동체를 떠난 간호대학생들의 대학생활체험

김고은,김유은,김희진,조유진,안주영,허다연,홍서우,차지영,박송이 이화여자대학교 간호학회 2018 이화간호학회지 Vol.- No.52

Purpose: The purpose of this study was to understand and explain the experiences of college life among junior nursing students who live apart from their families. Methods: 14 junior nursing students living apart from their families for at least one semester were recruited through snowballing and convenience sampling. Participants were interviewed individually using open ended questions. Data were collected from July to October in 2017 until data saturation. Interviews were transcribed verbatim and analyzed by using hermeneutic phenomenological method proposed by van Manen. Results: Five essential theme clusters emerged: (1) faced with diverse problems while living apart from family, (2) depend on family when stressed, (3) adapt to college life by being independent and forming self-initiative lifestyle, (4) confront with difficulties once again when adapting to academic curriculum restructured by practicum, (5) pave the way to nursing career and feel proud of overcoming the difficulties independently. Conclusion: Although most participants faced diverse problems in college life, they tried to adapt independently by forming self-initiative lifestyle and overturn the difficulties to positive experiences. In conclusion, the process of living apart from families transformed participants into proactive individuals. Adopting counseling programs could support starting their junior years successfully.

Development of Continuous Manufacturing Process for High-performance Thin Film Ni-YSZ Anode and Anode Functional Layer by Co-sputtering Method for Solid Oxide Fuel Cells

Y. J. Kim(김양재),S. Lee(이상훈),S. W. Cha(차석원) Korean Society for Precision Engineering 2021 한국정밀공학회 학술발표대회 논문집 Vol.2021 No.11월

The process of manufacturing an anode functional layer (AFL) in the conventional SOFC is inefficient, because, typically, the powder is ball-milled for a long time and sintered above 1,200°C. Also, in thin-film SOFCs, after fabricating the anode, a new target material or precursor is loaded on the vacuum deposition equipment to deposit the AFL. Alternatively, transferring the cell to other thin-film deposition equipment to lay the AFL requires more time and labor. Therefore, this study developed a process to fabricate an anode and AFL through the co-sputtering process continuously. By applying DC power to Ni and RF power to the Y/Zr metal alloy target, a Ni-YSZ anode of 700 nm and an AFL of 150 nm were continuously fabricated. This one-step fabrication process of an anode and AFL controls the proportion of the deposited material by adjusting DC and RF’s sputtering power. The film deposited with various power ratios was applied to anodic aluminum oxide wafers to characterize its electrochemical performance as SOFCs. XPS and EDS analyses were performed to confirm the compositional change according to the application of power. FESEM, AFM, and FIB-SEM analyses were performed to identify the microstructure change according to YSZ content.

Development of bulk heterojunction morphology by the difference of intermolecular interaction behaviors

Cha, H.,Baek, J.Y.,An, T.K.,Kim, S.O.,Kwon, S.K.,Kim, Y.H.,Park, C.E. Elsevier Science 2014 ORGANIC ELECTRONICS Vol.15 No.12

The morphology of a bulk heterojunction can be controlled by adding a processing additive in order to improve its power conversion efficiency (PCE) in photovoltaic devices. The phase-separated morphologies of blends of PONTBT or P3HT with fullerene derivatives are systematically examined in the presence of processing additives that possess various alkane alkyl chain lengths or end-group electronegativities. We determined the morphologies of the bulk heterojunction layers by using atomic force microscopy (AFM) and grazing incidence wide angle X-ray scattering (GIWAXS). The photocurrent-voltage characteristics of the bulk heterojunction solar cells were found to be strongly dependent on the intermolecular interactions between the conjugated polymers, the fullerene derivatives, and the processing additives in the photoactive layer. The optimal PONTBT:fullerene derivative blend morphology was obtained with a processing additive, 1,3-diiodopropane (1,3-DIP), that possesses a short alkyl chain and an end group with weak electronegativity, and was found to exhibit a high fill factor (FF) and a high current density (J<SUB>SC</SUB>). In contrast, in blends of P3HT with the fullerene derivative, PCEs with higher FF and J<SUB>SC</SUB> values were achieved by incorporating the processing additive, 1,8-dibromooctane (1,8-DBrO), which has a long alkyl chain and a strong electronegative end group. Thus the selection of the processing additive with the aim of enhancing photovoltaic performance needs to take into account the intermolecular interaction of the conjugated polymer.

Y염색체 장완 결실을 동반한 무정자증 1례

남윤성,김현주,이숙환,곽인평,윤태기,차광열,Nam, Y.S.,Kim, H.J.,Lee, S.H.,Kwak, I.P.,Yoon, T.K.,Cha, K.Y. 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.2

Different Y mutation in Yq11 occurring de novo in sterile males were first described 19 years ago. Since the phenotype of the patients was always associated with azoospermia or severe oligospermia, it was postulated that these mutations interrupt a Y spermatogenesis locus in the euchromatic Y region (Yq11) called azoospermia factor (AZF). Recently, it became possible to map AZF mutations to different subregions in Yq11by molecular deletion mapping. This indicated that azoospermia is possibly caused by more than one Y gene in Yq11 and the Yq11 chromatin structure. The frequency of AZF mutations in idiopathic sterile males $(5{\sim}20%)$ may indicate a need for a general screening programme for its analysis in infertility clinic. We have experienced a case of deletion distal to Yq11 region in azoospermic patient. So we report this case with a brief review of literatures.

운송하역 향상을 위한 Y/T 정차유도 프로토타입 장치 연구

김동훈(D. H. Kim),송준엽(J. Y. Song),차석근(S. K. Cha),강일용(I. Y. Kang),이승호(S. H. Lee) 한국정밀공학회 2009 한국정밀공학회 학술발표대회 논문집 Vol.2009 No.10월

Synthesis of triarylamine-based alternating copolymers for polymeric solar cell

Lee, J.,Cha, H.,Kong, H.,Seo, M.,Heo, J.,Jung, I.H.,Kim, J.,Shim, H.K.,Park, C.E.,Kim, S.Y. IPC Science and Technology Press 2014 Polymer Vol.55 No.19

Two donor-acceptor alternating copolymers based on electron-rich triarylamine, di(1-(6-(2-ethylhexyl))naphthyl)phenylamine (DNPA), and electron-deficient benzothiadiazole and benzoselenadiazole derivatives were designed and synthesized via Suzuki coupling reaction. The resulting triarylamine-based alternating copolymers PDNPADTBT and PDNPADTBS showed good solubility in common organic solvents and good thermal stability. The optical band gaps determined from the onset absorption were 1.93 and 1.81 eV, respectively. By introducing the naphthalene ring into the triarylamine, copolymers had relatively deep HOMO energy levels of -5.48 and -5.45 eV, which led to a high open circuit voltage (V<SUB>oc</SUB>) and good air stability for photovoltaic application. Bulk heterojunction solar cells were fabricated with a structure of ITO/PEDOT-PSS/copolymers-PC<SUB>70</SUB>BM/LiF/Al by blending the copolymer with PC<SUB>70</SUB>BM. Both blend systems showed remarkably high V<SUB>oc</SUB> near 0.9 V, and the highest performance of 2.2% was obtained from PDNPADTBT, with V<SUB>oc</SUB> = 0.88 V, J<SUB>sc</SUB> = 7.4 mA/cm<SUP>2</SUP>, and a fill factor of 34.4% under AM 1.5 G.

초등교사의 감정노동, 사회적 지지와 삶의 질 간의 관계

경예빈,김보민,김수정,노지현,송지윤,염소희,이현아,허유림,홍소연,차지영,박수민 이화여자대학교 간호과학대학 2017 이화간호학회지 Vol.- No.51

Purpose: The purpose of this study was to examine the correlation of emotional labor, social support, and quality of life among elementary school teacher. Methods: A correlational descriptive design was used with convenience sampling between July and December, 2016. The survey was self reported questionnaire administered to 130 elementary school teachers in the urban area. Data was statistically analyzed using t-test and one way ANOVA. Results: The average score of participants' emotional labor is 2.80, social support 3.89 and quality of life 3.65 of the total of 5. Emotional labor was negatively correlated to social support (r=-.178, p<.05) and quality of life(r=-.438, p<.001). Social support was positively correlated to quality of life (r=.336, p<.001). Conclusion: This study has significance as a basic data to understand the relation of emotional labor, social support, quality of life among elementary school teachers. Also, nursing interventions suitable for elementary school teachers should be developed to decrease the level of emotional labor, strengthen the social support, and enhance their quality of life.

EHP와 RRV의 Coinfection에 의한 Reassortants 선발에 관한 연구

유제현,조홍찬,송진욱,김유성,이영건,박선오,이종익,김응률,차광종,류영수,Harry B. Greenberg 건국대학교 동물자원연구센터 1998 動物資源硏究誌 Vol.19 No.-

본 연구는 두 종류의 rotavirus strain MA104 cell 상에서 동시감염시킨 후 gene이 서로 교차된 새로운 reassortants를 선발하기 위해 수행하였으며, 수행한 결과는 다음과 같다. 1. EHP T 와 RRV를 MA104 세포에 감염시켜 cytopathic effect(CPE)를 확인하였으며 확인된 virus를 수확하였다. 2. Coinfection의 mobility of infection은 각각 EHP T는 0.3(3*10?? PFU), RRV는 2.5(2.5*10?? PFU)이었다. 3. EHP T와 RRV를 coinfection하여 plaque assay에 의해 형성된 plaque들을 pick up하였다. 4. EHP T와 RRV를 동시감염시켜 얻어진 plaques를 MA104 cell에 재감염한 후 grow up하여 수확하였다. 5. Plaques를 24 Well의 MA104 cell에 감염하여 수확한 후 dsRNA를 추출하여 전기영동에 의해 26개 reassortants를 선발하였다. This study was carried out to select new reassortants whose genes were exchanged by confection of two strains. The results obtained in these experiments were as follows: 1. Cytopathic effect was checked out in the experiment in which made MA104 cells infected by EHP T (murine rotavirus) and RRV(simian rhesus rotavirus). And then the viruses were obtained from the procedure. 2. Mobility of infection(MOI) of EHP T and RRV were 0.3(3*10?? plaque forming units) and 2.5(2.5*10?? plaque forming units)respectively. 3. After coinfection of EHP T and RRV, plaques which formed in plaque assay were picked up. 4. Coinfected EHP T and RRV plaques were grown up in 24 well of MA104 cell and then grown-ups or those were obtained. 5. By polyacrylamide gel electrophoresis(PAGE) of dsRNA extracted above, 26 reassortants were selected.

Majarine의 중추신경계에 대한 작용(Ⅲ) : Modification of Tetrahydromajarine-induced Hypothermia by Drugs Affecting Brain Monoamine Functions in Mice Tetrahydromajarine의 마우스 체온하강 작용에 대한 뇌 Monoamine에 미치는 약물들의 작용

이종화,조병헌,김종배,김유재,김천숙,차영덕,김영석,박영현 순천향대학교 1986 논문집 Vol.9 No.2

한국 특산 매자나무(Berberis koreana Palibin)의 뿌리에서 분리한 majarine은 4급 ammonium의 isoquinoline 알칼로이드로서 본교실에서는 중추신경계에 대한 약리작용을 검토하고 있다. Majarine을 보다 지용성이고 뇌혈관 장벽(Blood-brain barrier)을 투과하기 쉬운 3급 amine인 tetrahydromajarine(THM) 으로 환원시켰다. Tetrahydromajarine을 마우스 복겅내 투여하여 직장온도 변화와 뇌 monoamine에 영향을 주는 약물들은 전처치하여 약물상호작용을 관찰하여 다음과 같은 성적을 얻었다. 그 성적을 통해서 tetrehydromajarine의 체온하강 작용에 대한 뇌 monoamine의 작용기전을 밝히고져 하였다. Tetrahydromajarine(0.5-50 mg/kg)은 마우스에 있어서 용량의존적으로 현저한 체온감소를 나타내었으나, 0.5mg/kg 투여시 체온하강의 유의성은 없었다. THM의 체온하강은 5-hydroxytryptamine(5-HT: serotonin) antagonist인 cyproheptadine인 5-HT depletor의 p-chlorophenylalanine에 의해 유의성있게 억제되었다. Dopamine(DA) antagonist인 haloperidol에 의해 THM의 체온하강이 억제되었으나, 50 mg/kg 투여시 완전하게 억제되지 않앗다. 5-HT와 noradrenaline(NE)의 reuptake inhibitor인 imipramine과 DA와 NE의 합성요소인 tyrosine hydroxylase inhibitor인 a-methyl-p-tyrosine의 전처치시 tetrahydromajarine의 체온하강을 완전하게 억제시키지 않았으나, THM 50 mg/kg 투여시 유의성을 나타내었다. Reserpine antagonism test에서 tetrahydromajarine(10-50 mg/kg) 전처치후 reserpine을 마우스 정맥내 투여시 hypothermia reversal 없이 용량 의존적인 체온감소를 나타내었다. 이러한 결과로 보아 tetrahydromajarine의 체온하강은 뇌 monoamine인 5-HT와 직접적인 관련성이 있고, dopamine도 연관된다고 사려된다. Majarine is the isoquinoline alkaloid with quaternary ammonium isolated from the root of Berberis koreana Palibin distributed only in Korea. Authors had investigated its pharmacological action on the central nervous system. Tetrahydromajarine(THM) with tertiary amine obtained by reduction of majarine is more lipid soluble and will be expected to penetrate into the blood-brain barrier. We report the results of a series of experiments to investigate further the role played by dopaminergic and serotonergic mechani는 in the action of tetrahydromajarine on body temperature in the mouse. Hypothermia was dose-dependent at a does of 0.5, 10 and 50mg/kg of tetrahydromajarine, but not significant at a does of 0.5mg/kg in mice. By pretreatment with 5-hydroxytryptamine antagonist, cyproheptadine and 5-HT depletor, p-chlorophenylalanine, the hypothermic effect of THM was blocked significantly or completely. Pretreatment with dopamine antagonist, haloperidol, completely blocked the hypothermia normally induced by tetrahydromajarine, but about 50% of the hypothermia induced by 50 mg/kg. Pretreatment with reuptake antagonist of 5-HT and noradrealine, imipramine and tyrosine hydroxylase(DA and NE synthesizer) inhibitor, a-methyl-p-tyrosine, did not completely blocked the tetrahydromajarine-induced hypothermia, but significantly at a does of 50 mg/kg. Reserpine-induced hypothermia and its reversal by tetrahydromajarine(10-50 mg/kg) was not significant and it produced a dose-dependent hypothermia. Regarding the mediation of the hypothermic effect of tetrahydromajarine in the brain monoamine functions, our results support a significant role of direct related 5-HT and DA.