Model 용액 중 향기성분 추출과 그의 농축 중 수율에 관한 연구

정숙현 東西大學校 2002 동서논문집 Vol.8 No.-

!0% hydro-alcoholic model solution containing 0.57ppm hexane, 0.71ppm heptanone-2, 0.55ppm ethyl hexanoate, 0.60ppm linalool, 0.58ppm 2-phenyl ethanol, 0.62ppm 2-phenyl ethyl acetate, 0.52ppm γ-nonalactone, 0.51ppm β-ionone, 0.64ppm ethyl dodecanoate, 0.52ppm ethyl hexadodecanoate and tridecane as internal standard was studied for extraction yield using 3L of continuous liquid-liquid extractor. This extraction was done with CCl_3F at 5℃±1(cold room). 300ml extract was concentrated to 0.1ml with Dufton column and the yields were gotten hexanol 81%, heptanone-2 92%, ethyl hexanoate 84%, linalool 60%, 2-phenyl ethanol 32%, 2-phenyl ethyl acetate 81%, γ-nonalactone 114%, β-ionone 84%, ethyl dodecanoate 78%, ethyl hexadecanoate 30%. For the efficiency of concetration column the solution was prepared 100ppm of ethyl propanoate, pentanone-2 and hexanone-2 in CCl_3F. More than 95% yield of concentration was gotten by first 3 differents size of Dufton column is using step by step(300ml → 0.5ml). But last Dufton column(0.5ml → 0.1ml) worked a little less efficiently than first 3 clumns(hexanone-2 93%, pentanone-2 85%, ethyl propanoate 82%). From this study analysis of alcoholic beverage flavour compounds can be done with CCl_3F as solevent, continuous liquid-liquid extractor and Dufton column.

탄소섬유강화플라스틱(CFRP) 선체소재와 전기추진체계가 소형선박의 경량화에 미치는 효과

정숙현,원준희,정승호,오대균 해양환경안전학회 2017 해양환경안전학회 학술발표대회 논문집 Vol.2017 No.11

비타민 D₂ 강화 표고버섯과 해조칼슘 첨가 냉면의 저장성 예측 - 연구노트 -

정숙현,오혜숙,윤교희,Chung, Sook-Hyun,Oh, Hae-Sook,Yoon, Kyo-Hie 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.9

비타민 $D_2$ 강화한 표고버섯(3%)과 해조칼슘을 첨가하여 비살균 제품이며 숙면에 속하는 냉면을 제조한 후, 편의식 개발을 목적으로 저장성을 예측하고자 하였다. $5^{\circ}C$에서 8일간 냉장저장하면서 미생물 및 관능적 품질 변화를 관찰한 결과, 냉면의 초기 세균 수는 2.54 log CFU/g 정도였으며, 냉장 조건에서 6일까지는 세균수가 5 log CFU/g 이하로서 식용에 적합하다고 판단되었다. 버섯냉면을 8일간 냉장저장 중에는 이취를 인식하지 못하였고 육안으로는 곰팡이 발생여부를 확인할 수 없었다. 표고버섯 첨가냉면을 총 8주 동안 냉동저장$(-18^{\circ}C)$하여 검사한 결과, 총세균수는 $770{\sim}160$ CFU/g 정도로 저장기간 중 유의적 차이를 보였으나 전 기간에 걸쳐 숙면의 저장가능 조건인 $1{\times}10^5$ CFU/g 이하로서 8주까지는 유통 및 저장 조건이 잘 유지된다면 미생물적 품질은 비교적 안전할 것으로 여겨진다. 윤기, 버섯 향, 쌉쌀한 맛, 씹힘성 및 탄력성의 관능특성은 8주간의 냉동저장 기간 동안 제조 직후와 비교할 때 유의적인 차이를 보이지 않았으며, 이 결과는 미생물검사 결과와 함께 저장 안정성이 매우 큼을 시사하는 것으로 여겨진다. Cold buckwheat noodles with 3% vitamin D2 enriched Siitake mushroom and seaweed derived calcium (Aquacal) added as functional ingredients were developed. For the prediction of shelf-life of these cold buckwheat noodles, classified as unsterilized cooked noodles, bacterial counts and sensory evaluations were examined during 8 days under refrigerated conditions (5oC) and 8 weeks of storage under frozen conditions $(-18^{\circ}C)$. Results of bacterial count showed that cold buckwheat noodles were microbiologically safe within 6 days at the storage temperature of $5^{\circ}C$ and the shelf-life of these noodles could be extended to 8 whole weeks when stored at $-18^{\circ}C$. Apparent qualities (off-flavor and fungi development) of cold buckwheat noodles kept in $5^{\circ}C$ were not decreased within 8 days. Sensory characteristics (gloss, mushroom flavor, bitterness, chewiness and elasticity) of noodles were not changed during 8 weeks at $-18^{\circ}C$.

거제도산 맹종죽(Phyllostachys edulis)추출물의 항균활성

정숙현 東西大學校 2000 동서논문집 Vol.6 No.-

The antibacterial activity of bamboo(Phyllostachys edulis : from Gueje island) extracts is investigated on 10 bacteria. The extracts are prepared using acetone, ethyl acetate, ethyl ether and methanol with roots, leaves and culms of bamboo. The cults extract showed the most effective antibacterial activity and the others are in the order of leaf and root extracts by hole-test. For the effect of extraction solvent on antibacterial activity, methanol is the most efficient to inhibit bacteria and the others result on in the order of acetone, ethyl ether and ethyl acetate in culms, ethyl ether, ethyl acetate and acetone in leaves. Among the bacteria V.parahaemoylticus is inhibited strongly by bamboo extracts and M.luteus, S.aureus, B.subtilis and K.pneumonia are also inhibited. MICs of M.luteus, K.pneumonia, and V.parahaemoylticus with methanol extract of cults are mesured 0.6%, 0.9%, and 1.2% (converted by fresh culm W/V) respectively. Lethal effect of methanol extract of 15g culm is observed incubating 100m1 media with 2 × 105 cfu/ml of M.luteus at 35℃. Decimal lethal time is calculation 78min. Key words : Bamboo extracts, Antimicrobial activity, MIC

운항조건을 고려한 소형선박의 환경영향평가 방법

정숙현,이동건,오대균 해양환경안전학회 2018 해양환경안전학회 학술발표대회 논문집 Vol.2018 No.11

「DER TOD IN VENEDIG」의 象徵的 意義

정숙현 이화여자대학교 독어독문학회 1971 Echo Vol.- No.1

포도주품질의 근원은 무엇인가? : 프랑스 포도주를 중심으로

정숙현 한국지역사회연구소 1993 地域社會 Vol.1993 No.2

F-35 Mycobacterial Characteristics and Treatment Outcomes in Extrapulmonary Mycobacterium abscessus Complex Infection

정숙현,김수영,허희재,기창석,이남용,강철인,정두련,백경란,신성재,고원중 대한결핵 및 호흡기학회 2016 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.121 No.-

Differentiation between Mycobacterium abscessus and M.massiliense and determination of macrolide susceptibility are crucial for management of patients with M.abscessus complex(MABC) infection. Unlike pulmonary MABC infection, little information on extrapulmonary MABC infection is available. The aim of this study was to evaluate proportion of M.massiliense among extrapulmonary MABC infections and that of C28 sequevar among extrapulmonary M.abscessus infections. We performed molecular identification of clinical isolates of MABC and assessed clinical characteristics and treatment outcomes of 23 patients with extrapulmonary MABC infection. The etiologic organism was M.abscessus in 11(48%) patients and M.massiliense in 12(52%). 8(73%) M.abscessus isolates that had inducible resistance to clarithromycin(CLR) harbored intact erm(41) gene and were of T28 sequevar, while 3(27%) M.abscessus isolates were of C28 sequevar and were susceptible to CLR. All M.massiliense isolates had deleted erm(41) and were susceptible to CLR. Of 21 patients who were available for analysis of treatment outcome, 18(86%) showed favorable outcome. The proportion of M.massiliense was higher than that of M.abscessus in patients with a favorable outcome(100% vs70%, P=0.090). All 3 patients with unfavorable outcome were infected with M.abscessus isolates that had intact erm(41). Both subspecies with nonfunctional erm(41) were associated with favorable outcome(P=0.042). Precise subspecies identification and determination of macrolide susceptibility are important for treatment of extrapulmonary MABC infection.

Comorbidity as a significant contributor to frequent severe acute exacerbation in COPD patients

정숙현,이현,정병호,이경종,전경만,엄상원,서지영,정만표,김호중,권오정,박혜윤 대한결핵 및 호흡기학회 2015 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.120 No.-

Background: Severe acute exacerbations (AEs) of chronic obstructive pulmonary disease (COPD) are associated with a more rapid decline in lung function, poor quality of life, considerable mortality, and high socioeconomic burden. Objective: We aimed to investigate risk factors associated with frequent severe AEs in COPD patients. Methods: A retrospective observational study was performed in 80 patients who experienced severe AE between February 2012 and January 2014 and had one-year follow-up from the first admission day. Results: Of 80 patients, 32 patients (40.0%) experienced frequent AEs (≥2 severe exacerbations over one year) and half of them experienced subsequent event within 90 days. Frequent severe AEs were associated with bronchial asthma, hypertension, poor lung function, home oxygen therapy and oral steroid medication, while subsequent severe AEs within 90 days after initial episode were related with bronchial asthma, poor lung function and home oxygen therapy. In multivariate analysis, home oxygen therapy (P = 0.025), low baseline FEV1 (P = 0.035), high CRP (P = 0.020), high body mass index (P = 0.005) and low eosinophil count (P = 0.049) were associated with frequent severe AEs, while bronchial asthma (P = 0.027) and home oxygen therapy (P = 0.040) were associated with early subsequent severe AEs. Conclusion: The present study showed impact of concomitant bronchial asthma on early subsequent severe AE and found that severity-related factors of underlying disease was associated with frequents severe AE over one year in COPD patients.

Job Satisfaction of Industrial Women Specializing in STEM in The Busan-Ulsan-Gyeongnam Region

정숙현,박성미 한국공학교육학회 2010 공학교육연구 Vol.13 No.2

A survey was conducted on 300 working women(one woman/company) who specialize in STEM(Science, Technology,Engineering and Mathematics) in the Busan region(south of Korea) to determine their job satisfaction. The overall responseof women working in industry is relatively positive although they do experience a certain limit as female workers. Theserious difficulties are long work hours and restriction of behavior in consequence of being the minority. Respondents anticipate an increase in number of working women in the STEM field due to the enforcement of women resource policies in Korea. Survey results about career development show that some of the most important factors in professionalism areresponsibility, gumption and cooperation. The expectation of promotion is very low. Sexual discrimination is distinctly appearing in wages and promotions. If they decide to quit their job in the midstream, the key reason is in the difficultiesof compatibility between home and occupation. Survey results about job satisfaction to work in industry reveal that 31.0%of respondents are satisfied because their job is consistent with their specialty; 30.7% of respondents are working wherethey wanted. Among respondents who intend to change their job, only 20.7% of respondents wanted to keep their specialties. Two factors involved in the respondents’ satisfaction during work are special technical achievement and practicalapplication of their knowledge. But, they are unsatisfied in terms of implementing their leadership.