산약(Disocorea batatas) 에탄올추출물의 L. gasseri, S. mutans, P. gingivalis에 대한 항균능과 성장억제 효과

윤현서,박충무 대한통합의학회 2023 대한통합의학회지 Vol.11 No.2

Purpose : In this study, to prove the antibacterial effect of Disocorea batatas, which is widely used for food, and to confirm the growth inhibitory effect, the antibacterial activity against L. gasseri, S. mutans, and P. gingivalis was verified. Based on this, it is intended to verify the utility as a preventive and therapeutic composition for dental caries and periodontal disease. Methods : RAW 264.7 cells were used to verify the cell survival rate and NO (Nitric Oxide) inhibitory effect on Disocorea batatas ethanol extract (DBEE). In order to verify the antibacterial effect against L. gasseri, S. mutans, and P. gingivalis, concentrations of 125, 250, and 500 ㎎/㎖ of DBEE were used and measured by the disk diffusion method. In order to confirm the growth inhibitory effect, the absorbance was measured at 600 ㎚ at 3, 6, 12, 18, and 24 hours using the liquid medium dilution method, and the growth inhibitory effect was measured compared to the control group. Results : The cell viability for DBEE was 91 % at 50 ㎎/㎖, and there was no cytotoxicity. The NO production inhibitory effect was shown from 10 μg/ml, and the higher the concentration, the greater the inhibitory effect. As for the antimicrobial effect using the disk diffusion method, the higher the concentration, the higher the antibacterial effect. At 125 ㎎/㎖ and 250 ㎎/㎖, S. mutans and L. gasseri showed high antimicrobial activity, and at 500 ㎎/㎖, the antibacterial effect was higher in L. gasseri. The growth inhibitory effect in DBEE was concentration-dependent as the higher the concentration, the higher the growth inhibitory effect, and all of them began to show growth inhibitory effects from 6 hours. Conclusion : Considering that it is widely used as an edible and medicinal material, Disocorea batatas has shown the potential to be used as a substance to prevent and alleviate dental caries and periodontal diseases, and it is believed that further research can be applied to oral health care products.

Antibacterial activity of Dioscorea batatas, Morus alba, and Tagetes erecta against E. coli and S. aureus

윤현서,박충무 대한의생명과학회 2023 Biomedical Science Letters Vol.29 No.3

In this study, the anti-bacterial activity of three medicinal herbs such as Dioscorea batatas, Morus alba, and Tagetes erecta, have been applied in oriental medicine against Escherichia coli and Staphylococcus aureus. The antibacterial activity of Dioscorea batatas ethanol extract (DBEE), Morus alba ethanol extract (MAEE), and Tagetes erecta ethanol extract (TEEE) was analyzed by disk diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) assays. As a result, three medicinal herbs exhibited antibacterial activity in a dose-dependent manner as well as MAEE and TEEE showed the most potent antibacterial activity followed by disk diffusion assay against E. coli and S. aureus, respectively. MIC against E. coli and S. aureus was 1.6 mg/mL of MAEE and TEEE as well as 0.2 mg/mL of TEEE. MBC against both strains was 5 mg/mL of DBEE and 2.5 mg/mL of TEEE. Consequently, three medicinal herbs in this study showed potent antibacterial activity through the inhibited growth of E. coli and S. aureus, which is considered as a potential candidate for cosmeceutic agents to attenuate the pruritus. In addition, antibacterial and antihistamine effects followed by the extraction solvents should be analyzed in future studies.

Huh7 간암세포에서 민들레 추출물의 상피간엽전환 억제를 통한 항전이 효과

윤현서,안현,박충무 대한통합의학회 2023 대한통합의학회지 Vol.11 No.3

Purpose : Epithelial‐to‐mesenchymal transition (EMT) is recognized as an important cellular response in metastatic proceduresand characterized by loss of cellular polarity as well as gain of mesenchymal features, which enables migration and invasion. Hepatocellular carcinoma (HCC) is one of the most common primary carcinomas in the liver and exhibits a poor prognosis due to frequent extrahepatic metastasis. Taraxacum officinale has been used for a long time in oriental medicine because of its various pharmacological activitiessuch as anti-rheumatic, anti-inflammatory, antioxidative, and anticarcinogenic activities. In this study, the anti-metastatic activity of T. officinale water extract (TOWE) and ethanol extract (TOEE) was investigated through the regulation of EMT in the Huh7 cells. Methods : The effects of TOWE and TOEE on migratory and invasive activities were investigated by wound healing and in vitro invasion assays. Western blot analysis was also applied to analyze protein expression levels associated with EMT and their upstream transcription factors in Huh7 cells. Results : TOWE and TOEE treatment potently inhibited migration and invasion of Huh7 cells compared to the untreated group. Both extracts treatment inhibited protein expression levels of N-cadherin, matrix metalloproteinase (MMP)-9, and vimentin while E-cadherin was significantly accelerated. In addition, the activated status of transcription factors, Snail, nuclear factor (NF)-κB, and zinc finger E-box binding homeobox (ZEB)1 was also inhibited with statistical significance. In comparison to both extracts, TOEE more potently attenuated migration, invasion, and EMT markers as well as their transcription factors in Huh7 cells than TOWE, which means that TOEE might possess more functional phytochemicals than TOWE. Conclusion : Consequently, TOWE and TOEEattenuated metastatic activity of hepatocellular carcinoma through the regulation of EMT markers and their transcription factors in Huh7 cells, which means that T. officinale might be a promising strategy for a chemopreventive agent against HCC metastasis.

해양경찰의 주관적 건강관련 인식수준과 행복지수

윤현서 사단법인 인문사회과학기술융합학회 2017 예술인문사회융합멀티미디어논문지 Vol.7 No.5

The purpose of this study was to examine the awareness level of the maritime police, which is one of special occupations, about their own health and the relationship between their awareness level of health and quality of life. The subjects in this study were 499 selected police officers in four different regions. A survey was conducted for seven months from September, 2013, and the collected data were analyzed. The findings of the study were as follows: As for the most common oral symptom, the largest group(30.3%) replied their teeth smarted when they drank cold water or brushed their teeth. Subjective awareness level of health became lower with age(p<0.001), and oral discomfort became worse with age as well(p=0.017). Subjective health awareness(p<0.001) and oral health awareness(p=0.041) were better among the unmarried respondents, and the subjective health awareness and oral health awareness of the respondents whose period of service was longer were lower. The health level of the police constables was higher(p<0.001), and the respondents whose income ranged from 3.01 to 4 million won felt the most oral discomfort(p=0.008). When their happiness indexes of five items were added up, the happiness indexes of the college graduates were highest(p=0.033), and this happiness index was higher among the respondents who were male, who were in their 20s, who were married, whose period of service was one year or less and between 11 and 20 years, who were inspectors or of higher position and whose income was 5.01 million won or more. Concerning happiness index in another single item, that was higher among the respondents who were female, who were in their 40s, who received graduate-school education, whose period of service was between six and 10 years, who were inspectors or of higher position and whose income was 5.01 million won or more. Therefore the preparation of educational programs that aim at increasing subjective awareness level of health in special occupations is expected to be of use for the enhancement of the quality of life. 본 연구는 특수 직업군의 하나인 해양경찰을 대상으로 건강인식수준을 파악하고 건강인식수준에 따른 삶의 질을 알아보고자 2013년 9월부터 7개월 동안 4개 지역에 근무하는 경찰관 499명을 대상으로 최종 분석한 결과 다음과 같다. 치과정기검진에 따른 증후증상에서는“찬물 마시거나 이를 닦을 때 이가 시리다.”에서 30.3%로 가장 높았고, 연령이 증가할수록 주관적 건강인식수준(p<0.001)이 나빴으며, 구강내 불편감은 증가하는 추세였다(p=0.017). 미혼에서 주관적 건강(p<0.001)과 구강건강인식수준(p=0.041)이 높았고, 근무기간이 길어질수록 나빴으며, 순경에서 건강수준이 높았고(p<0.001), 301-400만원에서 구강 내 불편감을 가장 많이 느꼈다(p=0.008). 5문항 행복지수에서 대학졸업이 가장 높았고(p=0.033), 5문항 행복지수는 남자, 20대, 기혼, 근무기간 1년 이하와 11-20년, 경감이상, 501만원 이상으로 높았으며, 단일문항 행복지수는 여자, 40대, 대학원졸, 근무기간 6-10년, 경감이상, 501만원 이상으로 높았다. 따라서 특수직종에 맞는 주관적 건강인식수준을 개선하기 위한 교육프로그램이 마련됨으로써 삶의 질의 향상에 도움이 되리라 사료된다.

40세 이상 성인 한국인에서 한국형 식사염증지표 수준에 따른 당뇨병 발생률 및 당화혈색소 수준 변화 연구

윤현서,손진영,박윤정 한국영양학회 2022 Journal of Nutrition and Health Vol.55 No.2

Purpose: The present study examined the associations of Korean Food-based Index of Dietary Inflammatory Potential (FBDI) scores with the prevalence of diabetes and hemoglobin A1c (HbA1c) level of diabetes patients in Korean adults. Methods: The Korean Genome and Epidemiology Study (KoGES) Health Examinee baseline data, collected between 2004 and 2013 and followed up between 2012 and 2016, were used in our study. A total 56,391 participants including diabetes (n = 5,733) and non-diabetes (n = 50,658) were analyzed. The subjects were classified into quartiles of FBDI scores using the semi-quantitative food-frequency questionnaire developed for KoGES. The prevalence rate of diabetes under FBDI scores was assessed by Cox proportional risk models and the severity of the diabetes was analyzed by multiple regression analysis. Results: There were 775 incident cases of diabetes after a mean follow-up of 3.97 years. There was no statistically significant association between FBDI scores and incidence of diabetes. Among diabetes patients at baseline, FBDI scores were related to the risk of progression of diabetes which was represented by greater than 9% HbA1c (Q1 vs. Q4; odds ratio, 1.562 [95% confidence intervals, 1.13–2.15]; p for trend = 0.007). The stratified analysis showed a stronger association in females, irregular exercise group, and higher body mass index group. Conclusion: These results suggest that a pro-inflammatory diet is not associated with the incidence of diabetes but is related to the HbA1c level of diabetes patients. Thus, further longitudinal studies with longer periods are required to determine a relationship between dietary inflammatory index and diabetes in Korea.

성인들의 스트레스 지수에 따른 구강자각증상

윤현서 사단법인 인문사회과학기술융합학회 2016 예술인문사회융합멀티미디어논문지 Vol.6 No.8

The purpose of this study was to examine the perceived oral symptoms of adults according to their perceived stress, and to compare their self-rated health and self-rated oral health. It's ultimately meant to provide some information on how to cope with stress to prevent diseases in case of being unable to relieve stress. The subjects in this study were the selected adults who were resided in Busan and South Gyeongsang Province and who were aged 20 and up. A survey was conducted for six months from March, 2015. There were significant differences in appearance(p=0.002) and interpersonal relationships(p=0.024) according to gender, and significant differences were found in appearance (p=0.034) and interpersonal relationships(p=0.005) according to residential area as well. And there were significant differences in work/studies (p<0.001) and appearance(p=0.001) according to age. The adults who were under heavier stress were more conscious of herpes febrilis(p=0.009), being dug in the inside of the mouth(p-0.017), the skin of the lips(p=0.011), having canker sores or being dug on the tongue (p=0.017), being unable to taste food(p=0.045) and having a cracked tongue(p=0.001), and they also were more conscious of toothache (p=0.007), pain around the jaw(p=0.001) and headache(p<0.001). As for self-rated health, the respondents who found themselves to be in better health were less stressed(p<0.001) and perceived less discomfort on the lips(p=0.038), on the tongue(p=0.035) and in the temporomandibular joint(p=0.001). Concerning self-rated oral health, the adults who found themselves to be in better oral health experienced less stress(p<0.001), and they felt less discomfort on the tongue(p=0.003), in the teeth and gums(p<0.001) and in temporomandibular joint(p=0.014). The differences between them and the others were significant. Therefore the development of programs that aim at improving self-rated health and reducing stress is required. 본 연구는 2015년 3월부터 6개월간 부산•경남에 거주하는 20세 이상의 성인을 대상으로 스트레스 인지에 따른 구강 내 자극증상에 대하여 알아보고 더불어 주관적 건강인식수준과 구강건강인식수준을 함께 비교함으로써 스트레스를 줄일 수 없다면, 적절하게 대처하고 처치하여 질환을 예방하기 위한 기초자료로 삼고자 한다. 성별에서는 외모(p=0.002), 인간관계(p=0.024), 지역에서는 외모(p=0034), 인간관계(p=0.005), 연령이 증가할수록 업무 및 학업(p<0.001)과 외모(p=0.001)에서 유의한 차이를 보였다. 스트레스가 높을수록 입술물집(p=0.009), 입안쪽 파임(p=0.017), 입술껍질(p=0.011) 혀는 헐거나 파임(p=0.017), 음식 맛 못 느낌(p=0.045), 혀 갈라짐(p=0.001)을 자각하고, 치아통증(p=0.007), 턱 주위의 통증(p=0.001), 두통(p<0.001)을 자각하였다. 주관적 건강수준 인식에서는 건강하다고 인식할수록 스트레스(p<0.001)수준이 낮았으며, 입술(p=0.038), 혀(p=0.035), 턱관절(p=0.001)였고, 주관적 구강건강수준 인식에서는 건강하다고 인식할수록 스트레스(p<0.001)수준이 낮았으며, 혀(p=0.003), 치아와 잇몸(p<0.001), 턱관절(p=0.014)에서 불편함을 자각하는 수준이 낮은 것으로 유의한 차이를 보였다. 따라서 주관적 건강인식수준 향상과 스트레스를 낮추기 위한 프로그램들이 개발되어야 할 것이다.

HaCaT 세포에서 며느리 배꼽 추출물의 AP-1, PI3K/Akt 및 MAPK 활성 조절을 통한 광손상 억제 효과

윤현서,박충무 대한통합의학회 2024 대한통합의학회지 Vol.12 No.1

Purpose : Skin is the primary barrier to protect the body from various exogenous factors. Among them, UVB exposure can cause the induction of not only excessive inflammatory responses but also the degradation of extracellular matrix (ECM), including collagen and elastin. This study tried to investigate the ameliorative effect of Persicaria perfoliata ethanol extract (PPEE) on UVB-irradiated photodamage through the regulation of activator protein (AP)-1, phosphoinositide 3-kinase (PI3K)/Akt, and mitogen-activated protein kinase (MAPK) signaling molecules in HaCaT cells. Methods : The cytotoxicity of PPEE on HaCaT cells was evaluated by the WST-1 assay. The 80 mJ/㎠ of UVB (312 ㎚) was irradiated on HaCaT cells to induce the photodamage. Western blot analysis was conducted to investigate the protein expression levels of cyclooxygenase (COX)-2, matrix metalloproteinase (MMP)-9, and heme oxygenase (HO)-1 for ameliorative status by PPEE treatment in UVB-exposed HaCaT cells. In addition, the activated status of the inflammatory transcription factor, AP-1, as well as upstream signaling molecules, PI3K/Akt, and MAPK, were also evaluated by Western blot analysis. Results : Any cytotoxic effect was not induced at the concentration up to 200 ㎍/㎖ by PPEE treatment. Protein expression levels of COX-2 and MMP-9 were significantly down- and up-regulated by PPEE treatment. The inflammatory transcription factor AP-1, stimulated by UVB irradiation, was also significantly attenuated by PPEE treatment. The phosphorylated status of PI3K/Akt and MAPK were mitigated by PPEE treatment in UVB-exposed HaCaT cells. Moreover, PPEE treatment potently accelerated the expression of HO-1 and its transcription factor, nuclear factor-erythroid 2-related factor (Nrf)2, which is known for its anti-inflammatory activity. Conclusion : Consequently, PPEE treatment significantly regulated COX-2 and MMP-9 expressions in UVB-irradiated HaCaT cells. The inflammatory transcription factor AP-1, along with upstream signaling molecules PI3K/Akt and MAPKs, were also attenuated by PPEE treatment in UVB-exposed HaCaT cells. Additionally, PPEE treatment exaggerated HO-1 expression and Nrf2 activation, which might have contributed to the anti-inflammatory activity of PPEE. These results indicate that PPEE could be a candidate for attenuating UVB-induced photodamage in human skin.

보육교사의 구강보건교육 및 칫솔질 지도에 따른 유아의 치면세균막 지수(QHI) 변화

윤현서,배수명,김혜진 국제차세대융합기술학회 2022 차세대융합기술학회논문지 Vol.6 No.1

본 연구는 보육교사의 주도적인 칫솔질 지도(칫솔질 교육, 칫솔질 지도)를 통해 유아의 치면세균막지수(Qugley-Hein indx) 변화를 살펴보고 구강건강 증진 방안을 모색하고자 시행하였다. 2020년 9월 8일부터 2021년 2월 18일까지 B시 D대학교 부설 어린이집 보육교사 4명과 원아 36명을 대상으로 보육교사의 주기적인 구강보건교육과 매일 점심 후 칫솔질 지도를 통해 치면세균막지수(Qugley-Hein indx)가 감소하는 것을 확인하였다. 전문가(치과위생사)의 보육교사 대상 구강보건교육으로 유아의 구강건강관리에 대한 관심과 필요성이 높아지는 긍정적인 효과를 확인하였으며 유아의 점심식사 후 칫솔질 지도를 통해 구강질환을 예방할 수 있는 긍정적인 효과를 확인하였다. 이를 통해 유아를 대상으로 구강관리를 좀 더 효율적으로 할 수 있는 보육교사의 교육 기회를 확대하고 관심을 유도할 수 방안이 모색되어져야 할 것이다. This study was conducted to examine changes in the Qugley-Hein indx of infants through the guidance of childcare teachers (tooth brushing education, brushing instruction) and to seek ways to improve oral health. From September 8, 2020 to February 18, 2021, the dental plaque index (Qugley-Hein indx) was confirmed to decrease. Oral health education for childcare teachers by experts (dental hygienists) confirmed the positive effect of increasing children's interest and need for oral health care. Confirmed. Through this, it is necessary to find ways to expand educational opportunities for childcare teachers who can do oral care more effectively for children and to induce interest.

창원시와 함안군 지역주민들의 주관적 구강건강인식수준 조사

윤현서,Yoon, Hyun-Seo 한국학교ㆍ지역보건교육학회 2018 한국학교지역보건교육학회지 Vol.19 No.1

The purpose of this study was to examine the levels of the subjective oral health awareness of local residents in the city of Changwon and Haman county in an effort to lay the foundation for the development of customized local oral health education programs. The 2015 community health survey data were used, and the data from 864 residents in Changwon and from 719 dwellers in Haman county were analyzed. The Changwon residents brushed their teeth after lunch more, received more oral checkups over the past year and had more scaling experience. The Haman residents had more experience of not receiving dental treatment. The subjective health awareness, subjective oral health awareness, chewing difficulty and periodontal health of the Haman dwellers were all worse, and all the levels of awareness were lower among the respondents who were older and who were diagnosed with hypertension and/or diabetes. The respondents who made use of dentures and who didn't brush their teeth after lunch had experience of not receiving dental treatment, and all the levels of health-related awareness were lower among the respondents who didn't receive dental checkups nor had scaling experience. To remedy the situation, efforts should be made to prevent and manage chronic diseases, and the establishment of a system that guarantees regular dental checkups and scaling is required. And the kind of environments that everybody can receive dental treatment when they want should be prepared. In order to change negative awareness, necessary measures should be taken to provide oral health education that is not temporary but sustainable and tailored to local characteristics and different life stages.

초등학생의 사회성 함양을 위한 한국 민속춤 프로그램 모형 연구

윤현서,윤혜선 한국예술교육학회 2019 예술교육연구 Vol.17 No.2

The purpose of this study is to develop a model program that applies Korean folk dance as the contents of dance education with the goal of enhancing social skills of elementary school students. To this end, prior research conducted on the model program of this study was analyzed and one-session structure that was deemed suitable for elementary school students was established. The details were then devised and after the content validity was reviewed by experts, the program model was revised and supplemented before a final version was developed. The results of the study are as follows. First, by conducting an analysis on previous research of the model program, the one-session structure was determined as 'creating, performing and appreciating'. Second, the step-by-step structure of the model program would consist of stages of "goal presentation - decision making - group expression activity - appreciation and sharing" which follows the characteristic analysis of cooperative learning method based on previous research in education engineering. The details of the program were determined via the analysis of previous research on Korean folk dance that were determined to be applicable to the program in this study, and the educational contents have been devised after the analysis of Korean folk dance characteristics. Third, in order to present the concrete application method of the model program developed by this study, ten dance programs were selected as examples of application of Korean folk dance. These selected dances include the mask dance, Kanggangsulae(dancing in one big circle), Sogo dance which are traditional group dances and together the selected example are representative of the diversity of the Korean folk dance that can be applied to the model. This study is valuable as it provides basic data that can be applied in elementary dance education by developing a systematic program of dance education that is based on Korean folk dance program for enhancing the social skills of elementary school students. It is hoped that this study serves as a useful resource for the research that raises the awareness of traditional culture and the social skills of elementary school students. 본 연구는 초등학생의 사회성 함양을 위하여 한국 민속춤을 무용교육의 내용으로 적용한 프로그램 모형을 개발하는 데 목적이 있다. 이를 위해 본 연구의 프로그램 모형 연구에 관한 선행연구를 분석하여 초등학생에게 적합한 1회기 구조와 단계별 구조 및 세부 활동 내용을 구성하고, 전문가 집단의 내용 타당도 검증을 통해 수 정·보완하여 최종적으로 프로그램 모형을 개발하였다. 이에 따른 연구 결과는 다음과 같다. 첫째, 무용 교육모 형 연구에 관한 선행연구 분석을 통해 본 프로그램 모형의 1회기 구조를 ‘창작하기, 수행하기, 감상하기’로 구 성하였다. 둘째, 본 프로그램 모형의 단계별 구조는 교육공학 분야의 선행연구를 토대로 협동학습방법의 특징 분석을 통해‘목표제시 - 의사결정 - 모둠 표현활동 - 감상 및 나눔’의 단계로 구성하였고, 프로그램의 세부 내용은 사회성 함양을 위한 한국 민속춤 관련 선행연구 분석을 통해 본 연구의 프로그램에 적용할 수 있는 한 국 민속춤의 특징을 분석하여 교육 내용으로 구성하였다. 셋째, 본 연구에서 개발한 프로그램 모형의 한국 민 속춤 종류에 따른 구체적인 적용방법을 제시하기 위해 한국 민속춤의 적용 예시로 탈춤을 선정하여 총 10차시 프로그램을 구성하였고, 탈춤 외에도 강강술래, 소고춤 등 집단 무용 성격의 민속춤 종류에 따라 사회성 함양 을 위한 다양한 한국 민속춤 프로그램으로 구성할 수 있는 적용 방안을 제시하였다. 본 연구는 초등학생을 대 상으로 사회성 함양을 위한 한국 민속춤 프로그램을 체계적인 구조의 무용교육 프로그램 모형으로 개발함으로 써 초등 무용교육현장에서 적용 가능한 기초 자료를 제공하였다는 점에서 의의가 있고, 향후 초등학생의 전통 문화의식 및 사회성 함양을 위한 연구에도 유용한 자료가 될 것이라 기대한다.