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GABA 생성능 우수 김치 젖산균 Lactobacillus sp. OPK2-59의 간 기능 개선 효과
배미옥(Mi-Ok Bae),김혜진(Hye-Jin Kim),차연수(Youn-Soo Cha),이명기(Myung-Ki Lee),오석흥(Suk-Heung Oh) 한국식품영양과학회 2009 한국식품영양과학회지 Vol.38 No.11
본 연구는 김치에서 분리한 GABA 생성능이 우수한 젖산균을 이용하여 알코올 투여 흰쥐의 간 기능 개선에 미치는 영향을 조사하였다. 실험동물의 체중 및 식이섭취량, 혈청 및 간 지질농도, 간 질환과 관련된 혈청 및 간 효소 활성, 혈중 에탄올 농도, 항산화 효소 활성을 측정한 결과는 다음과 같다. 체중 및 식이섭취량은 각 그룹간의 유의적인 차이를 나타내지 않았으며 각 기관 무게도 군 간의 유의적인 차이를 나타내지 않았으나 정상식이 섭취 군에 비해 알코올 투여 군에서 체중과 식이섭취량이 약간 감소하는 경향을 보였다. 알코올 섭취로 인해 높아진 혈중 총 콜레스테롤 및 중성지질은 젖산균 GABA 파우더투여 군에서 유의적인 차이를 보이며 정상식이 군과 비슷한 수준으로 낮아졌다. 또한 HDL-cholesterol은 군 간의 차이를 보이지는 않았으나, 알코올투여 군에 비해 높아지는 경향을 보였다. 간의 총콜레스테롤 농도는 군 간의 유의적인 차이는 없었으나, 중성지질 농도는 실험군 간의 현저한 차이를 보이며 알코올 투여 군에 비해 젖산균 GABA 파우더 투여 군에서 정상식이 군과 비슷한 수준으로 낮아졌다. 혈중 효소활성 GOT, GPT는 알코올 투여 군에 비해 젖산균 GABA 파우더투여 군에서 유의적인 차이를 보이며 정상수준으로 낮아졌으며, 간 GOT는 젖산균 GABA 파우더투여로 인해 현저한 차이를 보이며 알코올투여 군에 비해 낮아졌다. GPT는 각 그룹 간 유의적인 차이를 보이지 않았다. 혈중 알코올 농도를 시간별(0분, 30분, 60분, 120분, 240분, 360분)로 측정한 결과 그룹간의 유의적인 차이를 나타내지 않았으나 젖산균 파우더투여 군에서 알코올 대사를 촉진하는 경향을 보였다. 각 그룹간의 간 조직 중 SOD 활성은 알코올투여 군에 비해 젖산균 파우더투여 군과 젖산균 GABA 파우더 투여 군에서 유의적으로 높은 SOD 수준을 나타내었다. 본 실험을 통해 김치에서 분리한 젖산균 OPK2-59 GABA 파우더가 혈중 총 콜레스테롤과 중성지질, 간의 중성지질 농도를 유의적으로 감소시키고, 혈중 GOT, GPT, 간 GOT 효소활성 감소, 알코올대사 촉진, 항산화 효소활성을 상승시키는 효과를 확인하였다. 이는 김치유래의 젖산균 OPK2-59 GABA 파우더가 알코올투여 흰쥐의 지방대사 및 간 기능을 개선시키는데 기여할 수 있음을 보여주는 것으로, 향후 이러한 효능에 대한 심도 있는 기작연구와 더불어 다른 효능에 대한 연구가 기대된다. 또한 본 젖산균을 김치발효용 스타터로 활용하여 기능성식품을 개발하게 된다면 우수한 전통생물자원을 이용하여 국민건강 증진에 크게 기여할 수 있을 것으로 사료된다. This study investigated the effect of improved liver function in rats administered with ethanol by kimchi lactic acid bacteria with high GABA producing capacity. Sprague-Dawley male rats were divided into four groups; normal diet control (NC), ethanol control (EC), ethanol+Lactobacillus sp. OPK2-59 normal powder (EL1), ethanol+Lactobacillus sp. OPK2-59 GABA powder (EL2) and fed for 6 weeks. Analysis showed that there were no significant differences in body weight and feed consumption among the groups during the experimental period. Also, there were no significant differences in organ weight among the groups. The test results showed total cholesterol and triglyceride in the blood concentration that were increased by ethanol administration were significantly lowered in EL2 group. Liver triglyceride was also significantly lowered in the EL2 group compared with the EC group. Serum GOT and GPT, and liver GOT levels were significantly lower in the EL2 group compared with the EC group. Serum ethanol concentration was lower in the EL1 and EL2 groups compared with the EC group. SOD activities in liver were significantly increased in the EL1 and EL2 groups compared with the EC group. These results suggest that Lactobacillus sp. OPK2-59 GABA powder improves lipid and enzyme profiles of rats administered with ethanol.
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양성천,배미옥,김선희,정재근,하동룡,원영준,정선식,류필열 대한감염학회 2003 감염과 화학요법 Vol.35 No.5
목적 : 최근 Salmonella는 세균성 식중독의 주 원인균으로 매년 환자의 발생이 증가되고 있어 국가적인 감시가 필요한 전염성 세균이다. 특히 1999년도에는 S. Enteritidis에 의한 식중독이 전국적으로 크고 작은 집단발생이 있었으며 또한 꾸준히 산발적으로 발생하였다. 본 연구에서는 1999년도에 광주지역에서 발생한 식중독 환자로부터 분리된 S. Enteritidis의 분자역학적 특성을 밝히고자 한다. 방법 : 1999년도 광주지역에서 발생한 식중독환자의 분변가검물과 의심되는 식품으로부터 S. Enteritidis를 분리하였으며, 분리된 균주의 분자생물학적 특성을 조사하기위하여 plasmid DNA의 분석과 random amplified polymorphic DNA(RAPD)를 실시하였다. 결과 : 광주지역에서 4월, 7월과 10월에 3건의 집단 발생하여 286명의 환자로부터 203주의 S. Enteritidis가 분리되었으며 산발적으로 발생한 환자에서 16주, 음식으로부터 2주가 분리되었다. 분리된 모든 균주의 O 항원은 D1(1, 9, 12), 편모(H)항원의 phase Ⅰ은 g, m으로 Enteritidis 혈청형이었다. 항균제 감수성 검사에서 vancomycin을 제외한 대부분의 약제에 대해 감수성을 보였다. 분리된 균주의 plasmid를 분리하여 분석하였던 바 집단 식중독 환자로부터 분리된 균주는 동일하게 8, 6, 3.8kb의 3개의 plasmid를 보유하고 있었으며, 산발적으로 발생한 환자로부터 분리된 균주에서는 2주에서는 8, 6, 3.8kb의 plasmid를, 10주에서는 8, 6, 3.8, 2kb의 4개의 plasmid를 보유하였다. 또한 음식물로부터 분리된 2균주에서는 4개의 plasmid를 보유하고 있었다. 그러나 환자로부터 분리된 1주와 경상남도 함양에서 집단 식중독을 일으킨 균주에서는 plasmid를 보유하고 있지 않았다. 이들 분리균주를 보다 더 자세히 구별하기 위해 RAPD를 분석하였던 바 광주지역에서 분리된 균주는 동일한 특성을 보였으며 경남에서 분리된 균주와는 다른 특성을 보였다. 결론 : 이상의 성적으로 보아 1999년 광주에서 발생한 3건의 집단감염과 산발감염 환자에서 분리된 균주 대부분은 동일한 plasmid DNA와 RAPD 양상을 보여, 모두 동일 클론에 의한 감염으로 추정되었다. Background : During the past 10 years, there has been an increased incidence of gastrointestinal infections caused by salmonellae in Korea. In 1999, there were several outbreaks and sporadic occurrences of food borne infections due to Salmonella entenca serotype Enteritidis in Gwangju. Thus, there is a need for careful monitoring of its occurrence. Methods : Salmonella Enteritidis were isolated from feces samples of patients with foodborne diarrhea in Gwangju, 1999. We performed antigen typing, examination of biochemical properties, anibiotic susceptibility test, plasmid typing and RAPD analysis to characterize of S. Enteritidis isolates. Results : There were three Salmonella outbreaks (April, July, October), and 203 isolates of S. Enteritidis were isolated from the 286 patients. Eighteen isolates were obtained from the patients of sporadic occurrences. Antigenic types of the isolates were 0 antigen; D1 (1, 9, 12), H antigen phase 1; (g, m), serotype; Enteritidis. The isolates were susceptible to most of the antibiotics. We performed plasmid DNA analysis of the isolates, and the results showed 3 plasmids (8, 6, 3.8 kb) in 14 of 14 strains from outbreaks; 3 plasmids (8, 6, 3.8 kb) in 2 isolates from sporadic cases; 4 plasmids (8, 6, 3.8, 2 kb) in 10 isolates from sporadic occurrences, and 2 isolates from food specimens. However, 1 isolate from patients and 2 isolates from Ham-Yang, Kyung Nam, did not contain plasmids. RAPD analysis showed that all isolates from Gwangju in 1999 showed relatively uniform characteristics which were different from those derived from Ham-Yang, Kyung-Nam. Conclusion : The present data demonstrated that most food poisoning cases by S. Enteritidis in Gwangju, 1999, were originated from the same Salmonella Enteritidis strains.
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유전자 재조합 Human Factor VIII(GC-γ AHF)의 안전성에 관한 연구
김민영,손장원,신민기,배미옥,김현우,최진혁,김준성,문서현,김정현 한국독성학회 2002 Toxicological Research Vol.18 No.1
This study was conducted to evaluate the safety of a recombinant human Factor VIII(GC-$\gamma$ AHF) manufactured by Korea Green Cross Company with different technology according to the Regulation of Korean Food and Drug Administration (l 998. 12. 3). In acute toxicity test, both genders of Sprague-Dawley rats and Beagle dogs were administered intravenously with GC-$\gamma$ AHF of three doses (3,125, 625 and 125 IU/kg), and single dose of 3,125 IU/kg, respectively. No dead animal and abnormal autopsy findings were found in Control and GC-$\gamma$ AHF treated group. Therefore, the 50% lethal dose ($LD_{50}$) of GC-$\gamma$ AHF was conidered to be higher than 3,125 IU/kg in rats and dogs. In the four weeks repeated intravenous toxicity study, GC-$\gamma$ AHF was administrated intravenosly to both genders of rats and dogs with 3 doses (500, 150, 50 IU/kg). There were neither dead animals nor significant changes of body weights during the experimental Period. In addition, no significant GC-$\gamma$ AHF related changes were found in clinical sign, urinalysis and other finding. Statistically changes were observed in hematological, biochemical and organ weight parameters of treated groups: however these changes were not dose dependent. No histopathological lesion were observed in both control and treated animals. Above data suggest that no observed adverse effect level of test materials in rats and dogs might be over 500 IU/kg/day in this study. In ocular irritation test, any injury on iris, conjunctiva and cornea in rabbits were not observed. The acute ocular irritation index (A.O.I.), mean ocular irritation index (M.O.I.) and Day-7 individual ocular irritation Index (I.O.I.) of GC-$\gamma$ AHF were 0. In the primary skin Irritation test, the primary irritation index (P.I.I.) oj GC-$\gamma$ AHF were 0. Therefore, the GC-$\gamma$ AHF is considered not to have the primary skin and eye toxicity in rabbits. In active systemic anaphylaxis (ASA) test, GC-$\gamma$ AHF and GC-$\gamma$ AHF emulsified with Freund's complete adjuvant (FCA) did not induce any symptom of anaphylactic shock in guinea pigs. In passive cutaneous anaphylxis (PCA) test, after sensitization with antisera of GC-$\gamma$ AHF sensitized mice, blue spots were observed on the hypodermis of back of rats, but diameter of each spot was smaller than 5 mm in each test groups except the positive control group. Based on the results of this study, GC-$\gamma$ AHF is not conidered to have any antigenic potential. In conclusion, at levels of up to 500 IU/kg, GC-$\gamma$ AHF did not produce treatment-related toxicity under the conditions of these acute-, four week repeated-toxicity, primary skin and eye toxicity, and antigenicity test.
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유전자 재조합 Human Factor Ⅷ(GC-γ AHF)의 안전성에 관한 연구
김민영,손장원,신민기,배미옥,김현우,최진혁,김준성,문서현,김정현,이소라,변종환,변태호,김정헌,조명행 한국독성학회 2002 Toxicological Research Vol.18 No.1
This study was conducted to evaluate the safety of a recombinant human factor Ⅷ(GC-γ AHF) manufactured by Korea Green Cross Company with different technology according to the Regulation of Korean Food and Drug Administration (1998.12.3). In acute toxicity test, both genders of Sprague-Dawley rats and Beagle dogs were administered intravenously with GC-γ AHF of three dosee (3,125,625 and 125 IU/kg), and single dose of 3,125 IU/kg, respectively. No dead animal and abnormal autopsy findings were found in control and GC-γ AHF treated group. Threrfore, the 50% lethal dose (LD_(50)) of GC-γ AHF was considered to be higher than 3,125 IU/kg in rats and dogs. In the four weeks repeated intravenous toxicity study, GC-γ AHF was administrated intravenously to both genders of rats and dogs with 3 doses (500, 150, 50 IU/kg). There were neither dead animals nor significant changes of body weights during the experimental period. In addition, no significant GC-γ AHF related changes were found in clinical sign, urinalysis and other finding. Statistically changes were observed in hematological, biochemical and organ weight parameters of treated groups; however, these changes were not dose dependent. No histopathological lesions were observed in both control and treated animals. Above data suggest that no observed adverse effect level of test materials in rats and dogs might be over 500 IU/kg/day in this study. In ocular irritation test, any injury on iris, conjunctiva and cornea in rabbits were not observed. The acute ocular irritation index (A.O.I.), mean ocular irritation index (M.O.I.) and Day-7 individual ocular irritation index (I.O.I.) of GC-γ AHF were 0. In the primary skin irritation test, the primary irritation index (P.I.I.) of GC-γ AHF were 0. Therefore, the GC-γ AHF is considered not to have the primary skin and eye toxicity in rabbits. In active systemic anaphylaxis (ASA) test, GC-γ AHF and GC-γ AHF emulsified with Freund''s complete adjuvant (FCA) did not induce any symptom of anaphylatic shock in guinea pigs. In passive cutaneous anaphylaxis (PCA) test, after sensitization with antisera of GC-γ AHF sensitized mice, blue spots were observed on the hypodermis of back of rats, but diameter of each spot was smaller than 5 mm in each test groups except the positive control group. Based on the results of this study, GC-γ AHF is not considered to have any antigenic potential. In conclusion, at levels of up to 500 IU/kg, GC-γ AHF did not produce treatment-related toxicity under the conditions of these acute-, four week repeated-toxicity, primary skin and eye toxicity, and antigenicity test.
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