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Overexpression of Nuclear Factor Y Subunit StNF-YA7 Enhances Drought Tolerance in Potato
나종국,김둘이,김경화,성은수,김범기,조광수,조지홍,박수권 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.2
Drought, the most devastating abiotic stress, has detrimental effects on crop production. To minimize suchadverse effects, it is crucial to improve drought tolerance in crops. Several members of the plant Nuclear Factor Y(NF-Y) gene family are involved in drought tolerance. In this study, we isolated the potato NF-Y subunit A 7(StNF-YA7) gene from Solanum tuberosum ‘Superior’ and analyzed its relationship to abiotic stress tolerance. StNF-YA7was induced by drought, salt stress, and abscisic acid (ABA), and a transient expression assay using 35S:StNF-YA7-smGFPshowed that StNF-YA7 is localized to the nucleus, as reported for other plant NF-Ys. To examine the involvementof StNF-YA7 in drought tolerance, we generated 35S:StNF-YA7 transgenic potato plants. The transgenic potato plantsexhibited enhanced drought tolerance, likely due to reduced leaf water loss. Analysis of the StNF-YA7 promoter(pStNF-YA7) fused to GUS revealed that StNF-YA7 is strongly expressed in vascular tissue. Taken together, theseresults suggest that enhancing drought tolerance via StNF-YA7 overexpression is associated with reduced leaf waterloss, likely through mechanism(s) involving the vascular system.
나종국,Mi-Hye Seo,Seok-Jun Moon,In-Sun Yoon,Yeon-Hee Lee,Jae Kwang Kim,Kyun Oh Lee,Dool-Yi Kim 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.4
Polycomb group (PcG) proteins form Polycomb Repressive Complex 2 (PRC2), which regulates seed development by the epigenetic control of gene expression. Interaction assay among Arabidopsis Fertilization-independent- seed2 (FIS) class PcG proteins showed that Fertilization- independent endosperm (FIE) interacts with Medea (MEA), a SET-domain polycomb protein, of which N-terminal region is crucial for the interaction. In this study, rice SET-domain PcG protein OsEZ1, also known as OsiEZ1 in indica rice, was analyzed to identify an interacting domain of OsEZ1 required for OsEZ1–OsFIE2 protein interaction. A series of OsEZ1 deletions were generated and used to determine an interacting domain of OsEZ1 with OsFIE2 using the yeast two-hybrid system. Among OsEZ1 deletions, only OsEZ1D2 and OsEZ1D3 interacted with OsFIE2, indicating that the 155K–169R or N-proximal region of OsEZ1 is crucial for OsFIE2–OsEZ1 interaction. To examine the physiological roles of OsEZ1, 35S:OsEZ1 Arabidopsis lines were generated. OsEZ1 overexpressors exhibited altered seedling growth and seed size, implying that OsEZ1 may play important roles in seedling and seed development.
Transcriptome analysis of a medicinal plant, Pistacia chinensis
나종국,최기영,박덕환,성은수,이상우,Jin Hang,Li Wan Yi,김종화 한국식물생명공학회 2019 JOURNAL OF PLANT BIOTECHNOLOGY Vol.46 No.4
Pistacia chinensis Bunge has not only been used as a medicinal plant to treat various illnesses but its young shoots and leaves have also been used as vegetables. In addition, P. chinensis is used as a rootstock for Pistacia vera (pistachio). Here, the transcriptome of P. chinensis was sequenced to enrich genetic resources and identify secondary metabolite biosynthetic pathways using Illumina RNA-seq methods. De novo assembly resulted in 18,524 unigenes with an average length of 873 bp from 19 million RNA-seq reads. A Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation tool assigned KO (KEGG orthology) numbers to 6,553 (36.2%) unigenes, among which 4,061 unigenes were mapped into 391 different metabolic pathways. For terpenoid backbone and carotenoid biosynthesis pathways, 44 and 22 unigenes encode enzymes corresponding to 30 and 16 entries, respectively. Twenty- two unigenes encode proteins for 16 entries of the carotenoid biosynthesis pathway. As for the phenylpropanoid and flavonoid biosynthesis pathways, 63 and 24 unigenes were homologous to 17 and 14 entry proteins, respectively. Mining of simple sequence repeat identified 2,599 simple sequence repeats from P. chinensis unigenes. The results of the present study provide a valuable resource for in-depth studies on comparative and functional genomics to unravel the underlying mechanisms of the medicinal properties of Pistacia L.
Involvement of rice Polycomb protein OsFIE2 in plant growth and seed size
나종국,서미혜,윤인순,이균오,이연희,김둘이 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.4
Seed development is a complex but orchestrated process that requires the fine-tuning of parentally governed gene expression, which is regulated by Polycomb proteins. Over the last decade, various Polycomb proteins have been identified and functionally characterized in plants, and it has been found that they form the PRC2suppressor complex, which is involved in various developmental programs, including seed development. In this study, the function of the rice fertilization-independent endosperm gene OsFIE2, which expresses a protein homologous to the Arabidopsis Polycomb protein FIE, was characterized. We also characterized OsEZ1/OsiEZ1,another key component of the PRC2 complex. Both the OsFIE2 and OsEZ11 genes are strongly expressed in leaf and stem compared to other tissues, including root, anther,ovary, and ovule. We further examined whether OsFIE2interacted with OsEZ1 using a yeast two-hybrid system. Interaction analysis showed that OsFIE2 interacted with OsEZ1 but not with Arabidopsis MEA protein. To examine the physiological roles of OsFIE2, 35S:OsFIE2 Arabidopsis lines were generated. Transgenic plants with 35S:OsFIE2 grew faster than wild-type plants during early development. Importantly, they produced bigger seed than the wild type, indicating that OsFIE2 may play an important role in seed size. In addition, we generated pOs-FIE2:GUS plants to examine the spatial expression pattern of OsFIE2. GUS expression was detected in cotyledon but not in any other tissues, suggesting that OsFIE2 expression may be required to suppress homeotic genes in cotyledon.
유색칼라 ‘Florex Gold’의 대량증식과정에서 auxin류가 발근에 미치는 영향
박형신,나종국,정찬선 한국국제농업개발학회 2013 韓國國際農業開發學會誌 Vol.25 No.2
1. ‘Florex Gold’ 품종의 측아 기내배양에서 auxin류의 단용처리는 multiple shoot 발생을 유도하지 못했다. Callus 형성은 2,4-D 1.0 mg/L 처리구에서 63.3%로 가장 높았고, multiple shoot 형성은 cytokinin인 zeatin 1.0 mg/L 처리구에서 평균 8.1개로 가장 높게 나타났다. 2. ‘Florex Gold’의 증식 실험에서 callus 형성은 NAA 1.0 mg/L와 BA 1.0 mg/L이 혼용처리된 MS 배지에서 26(86.7%)개의 절편체에서 발생해 가장 높았다. 또한 동일한 배지 조건이 multiple shoot발생에도 가장 효과적이었고, 평균 16.9개(평균길이4.6 cm)의 신초가 발생하였다. 3. ‘Florex Gold’ 신초의 발근유도 실험에서 2,4-D 1.0 mg/L처리가 절편체당 평균 6.7(3.2 cm)개의 뿌리를 유도하여 ‘Florex Gold’ 신초의 발근 유도에 가장 적합한 것으로 나타난 반면 NAA는 발근유도에 부적합 한 것으로 나타났다. Calla lily bulbs are prone to decaying disease above 28oC. Therefore, it is very difficult to produce or obtain a good commercial calla lily bulb at low price in temperate regions with hot summer. In such regions, calla lily seedlings from in-vitro micro-propagation can be used alternatively. In this study, we carried out experiments to establish appropriate in-vitro micro-propagation conditions of calla lily 'Florex Gold'. MS medium containing NAA 1.0 mg/L and BA 1.0 mg/L was optimal for both callus and multiple shoot formation. In the given MS media, 26 (86.7%) explants formed callus and all 30 explants produced multiple shoots with average of 16.9 shoots per explant. Obtained shoots were used for root induction on MS media supplemented with NAA, IAA, or 2,4-D respectively to determine an appropriate auxin for rooting. The best rooting was observed in MS media supplemented with 1.0 mg/L of 2,4-D, in which an average of 6.7 roots were generated per explant, indicating that 1.0 mg/L of 2,4-D is optimal for rooting of shoots derived from lateral buds of ‘Florex Gold’.