신경회로망을 이용한 순환식 돈분처리 시스템의 모니터링

고성철,손준일,최정혜,이민호 한국센서학회 2000 센서학회지 Vol.9 No.2

We propose a novel monitoring system for a recycling piggery slurry treatment system through neural networks. Here we tried to model treatment process for each tank(influent, fermentation, aeration, first sedimentation and fourth sedimentation tanks) in the system based on population densities of heterotrophic and lactic acid bacteria. Principle component analysis(PCA) was first applied to identify a relation between input(microbial densities and parameters for the treatment) and output, and then multilayer neural networks were employed to model the treatment process for each tank. PCA filtration of input data as microbial densities was found to facilitate the modeling procedure for the system monitoring even with a relatively lower number of input. Neural networks independently trained for each treatment tank and their subsequent combinatorial data analysis allowed a successful prediction of the treatment system for at least two days.

지방재정조정제도 운영현황에 대한 분석 : 기초지방자치단체 예산의 자주성과 건전성 측면을 중심으로 Focused on the aspects of self-reliance and soundness of the public finance of lower-level local governments

고성철,이승우 한국지방행정연구원 2003 地方行政硏究 Vol.17 No.2

This study analyzed the effect of the coordination system of local public finance on the operation of local public finance by utilizing a pooled regression analysis (TSCSREG: time series cross section regression analysis) using data collected during a 7-year period(1996-2002). To accomplish the objective, the figures for the variables of the coordination system of local public finance--Local Allocation Tax, Local Transfer Fund, National Subsidy and for the four variables on the aspects of self-reliance and soundness among different operational types of local public finance--the ratio of non-earmarked fund source, the ratio of own revenues, the ratio of investment, the ratio of systematic operation of local public finance were collected and analyzed. The results of the new analyses are as follows: (1) The ratio of non-earmarked financial source is likely affected negatively by the Local Allocation Tax of the coordination system of local public finance, while the Local Transfer Fund and the National Subsidy seem to have a positive effect; (2) In terms of the effect of the coordination system of local public finance on the ratio of own revenues, the results showed that the Local Allocation Tax and the National Subsidy affected negatively, whereas the Local Transfer Fund had a positive effect; (3) The effect of the coordination system of local public finance on the investment ratio of local public finance showed that the Local Allocation Tax, the Local Transfer Fund, and the National Subsidy all had positive effects; (4) Its effect on the ratio of systematic operation of local public finance indicated a positive sign with the Local Transfer Fund, a negative sign with the Local Allocation Tax and the National Subsidy.

경기도 중부지역의 식물상

고성철,신영화 한국자원식물학회 2009 한국자원식물학회지 Vol.22 No.1

Floral study on the vegetation of 8 mountains including Taehwa-san, Mugab-san, Haehyeob-san, Guksa-bong, Gwanggyo-san, Samseong-san, Suri-san, and 200m peak neighboring to Mulwang lake was carried out from April to October, 2007. They belong to the middle part of Gyeonggi Province, and located between Lat. 37°13′31.19″~37°33′3.48″, Long. 26°43′04.1″~127°26′28.38″. Vascular plants collected in these areas were total 447 taxa composed of 386 species, 5 subspecies, 46 varieties and 10 forms of 262 genera under 92 families. The area from which the most plentiful plants were found was Mt. Gwanggyo-san. The areas with comparatively excellent vegetation are easy slopes nearby valleys in Mt. Gwanggyo-san, Mt. Suri-san and Mt. Haehyeob-san. Forests of the examined areas are generally mixed of Pinus densiflora and Quercus plants, but herbaceous plants covering soil are becoming nearly extinct by air and soil pollutions except some sites. Families with abundant species are Compositae, Rosaceae, Liliaceae and Graminae, etc. Endemic plants found in these areas are 8 taxa of Clematis brachyura, Euonymus trapococcus, Viola seoulensis, Ajuga spectabilis, Scutellaria insignis, Weigela subsessilis, Aster koraiensis, Aconitum chiisanense and rare and endangered plants are 7 taxa of Arisaema heterophyllum, Iris odaesanensis, Eranthis stellata., Aconitum chiisanense, Prunus yedoensis (cultivar), Viola albida, and Syringa wolfi. As to useful plants, 192 taxa for the edible, 132 taxa for the medicinal, 130 taxa for the ornamental and 11 taxa for the staining were classified respectively. Among 17 taxa of specially designated plants, 5th degree plants are 2 taxa of Iris odaesanensis and Prunus yedoensis (cultivar), 4th degree plants are 2 taxa of Symplocarpus renifolius and Syringa wolfi, 3rd degree plants are 13 taxa of Dryopteris gymnophylla, Juniperus chinensis, Betula chinensis, Betula davurica, Diarrhena fauriei, Aconitum longecassidatum, Eranthis stellata, Spiraea salicifolia, Acer palmatum, Vaccinium koreanum, Scutellaria insignis, Weigela florida and Adoxa moschatellina. 경기도 중부지역의 북위 37°13′31.19″~37°33′3.48″, 동경 26°43′04.1″~127°26′28.38″사이에 해당되는 태화산(624 m), 무갑산(571.8 m), 해협산(531.3 m), 국사봉(540 m), 광교산(582 m), 삼성산(481 m), 수리산(474.8 m), 물왕저수지 인근 200고지(200 m)의 8개 지역의 관속식물 식물상을 2007년 4월부터 10월까지 조사하였다. 생육이 확인된 관속식물의 총 분류군수는 92과 262속 386종 5아종 46변종 10품종으로서 총 447분류군이었다. 가장 많은 식물종이 확인된 지역은 광교산이며, 식생이 비교적 양호한 지역은 광교산, 수리산, 해협산이고, 주로 계곡 주변의 완만한 사면부이다. 조사지는 전반적으로 소나무와 Quercus류가 우세한 혼효림이며, 일부지역을 제외하고 임상의 초본식생은 토양과 대기의 오염에 의해 거의 절멸상태에 가깝다. 비교적 많은 분류군이 채집된 과는 국화과, 장미과, 백합과, 벼과 식물 순이다. 본 조사지에 생육이 확인된 특산식물은 외대으아리, 지리바꽃, 버들회나무, 서울제비꽃, 자란초, 광릉골무꽃, 병꽃나무, 벌개미취 등의 8분류군이며, 희귀 및 멸종위기식물은 두루미천남성, 노랑무늬붓꽃, 너도바람꽃, 지리바꽃, 왕벚나무(식재), 태백제비꽃, 꽃개회나무 등의 7분류군이었다. 조사된 식물 중 유용식물로는 식용식물 192분류군, 약용식물 132분류군, 관상용식물 130분류군, 염료식물 11분류군이었으며, 귀화식물은 22분류군이었다. 식물구계학적 특정식물종은 5등급의 노랑무늬붓꽃, 왕벚나무(식재)의 2분류군, 4등급의 앉은부채, 꽃개회나무의 2분류군, 3등급의 금족제비고사리, 개박달나무, 광릉용수염, 너도바람꽃, 산앵도나무, 연복초 등 13분류군이었다.

국고보조금과 지방정부 사업평가의 연계 방안에 관한 연구: 중요국책사업에 대한 합동평가와의 연계를 중심으로

고성철 대한지방자치학회 2006 한국지방자치연구 Vol.8 No.1

A Case of Bilateral Bronchial Foreign Body

고성철,Jaeha Lee,Loek Sothearith,박기남 대한후두음성언어의학회 2022 대한후두음성언어의학회지 Vol.33 No.3

Foreign body (FB) aspiration remains a serious health problem, particularly in children. The complications due to occlusion by FB or related to procedures for removal can lead to morbidity and mortality. Most of the FBs are located in the unilateral bronchus, however, the organic FB can be multiple or bilaterally located when children chewed the FB. Here, we present a case of successful retrieval of bilateral bronchial FBs. Preoperatively, FB in the left main bronchus was diagnosed, however, FB of the right upper bronchus was not definitive on X-ray and CT scan. During the rigid bronchoscopy, occult FB in the right upper bronchus was detected and successfully removed.

Influence of Effective Microorganisms on Polluted Marine Sediment and Its Microbial Community

고성철,김병혁,배환진,권성현,최정혜,김재우 한국항해항만학회 2009 한국항해항만학회지 Vol.33 No.2

Lactobacillus sp., Acetobacter sp. and yeast were the most dominant organisms in the EM stock culture and subculture product. Lactic acid bacteria and yeast were able to grow in the fermentation process utilizing seawater. EM treatment of higher concentrations using EM stock culture and EM clay balls (1% or 4%) contributed to an early removal of malodor and an increase of DO in the polluted sediments, indicating an odor-removing activity of EM. The EM treatment of higher concentrations (1% or 4%) somewhat appeared to modify the microbial communities within the sediments, which was confirmed by existence of a few unique fragments from the stock culture based on PCR-DGGE. It still remains to be elucidated that EM cultures were directly involved in the malodor removal and potential sediment bioremediation. Lactobacillus sp., Acetobacter sp. and yeast were the most dominant organisms in the EM stock culture and subculture product. Lactic acid bacteria and yeast were able to grow in the fermentation process utilizing seawater. EM treatment of higher concentrations using EM stock culture and EM clay balls (1% or 4%) contributed to an early removal of malodor and an increase of DO in the polluted sediments, indicating an odor-removing activity of EM. The EM treatment of higher concentrations (1% or 4%) somewhat appeared to modify the microbial communities within the sediments, which was confirmed by existence of a few unique fragments from the stock culture based on PCR-DGGE. It still remains to be elucidated that EM cultures were directly involved in the malodor removal and potential sediment bioremediation.

Inhibitory Effect of Bee Venom Toxin on the Growth of Cervix Cancer C33A Cells via Death Receptor Expression and Apoptosis

고성철,송호섭 대한침구의학회 2014 대한침구의학회지 Vol.30 No.3

Objectives : We investigated whether bee venom(BV) inhibit cell growth through enhancement of death receptor expressions in the human cervix cancer C33A cells. Methods : BV(1~5 ㎍/㎖) inhibited the growth of cervix cancer C33A cells by the induction of apoptotic cell death in a dose dependent manner. Results : Consistent with apoptotic cell death, expression of Fas, death receptor(DR) 3, 4, 5 and 6 was increased concentration dependently in the cells. Moreover, Fas, DR3 and DR6 revealed more sensitivity to BV. Thus, We reconfirmed whether they actually play a critical role in anti-proliferation of cervix cancer C33A cells. Consecutively, expression of DR downstream pro-apoptotic proteins including caspase-8, -3, -9 was upregulated and Bax was concomitantly overwhelmed the expression of Bcl-2. NF-κB were also inhibited by treatment with BV in C33A cells. Conclusions : These results suggest that BV could exert anti-tumor effect through induction of apoptotic cell death in human cervix cancer C33A cells via enhancement of death receptor expression, and that BV could be a promising agent for preventing and treating cervix cancer.

다염화비페닐 (polychlorinated biphenyls , PCBs) 의 환경정화에 있어서 테르핀 (terpenes) 에 의한 생분해촉진기작의 분자생태학적 규명

고성철,김병혁,정경자,소재성,김응빈,김종설 대한환경공학회 2002 대한환경공학회 학술발표논문집 Vol.2002 No.12

Selection of Molecular Biomarkers Relevant to Abnormal Behaviors of Medaka Fish (Oryzias latipes) Caused by Diazinon

고성철,신성우,조현덕,전태수,김정상,이성규 환경독성보건학회 2009 환경독성보건학회지 Vol.24 No.4

본 연구의 목적은 다이아지논(Diazinon; O, O-diethyl O-[6-methyl-2 (1-methylethyl)-4-pyrimidinyl] phosphorothioate)에 노출된 모델 생물체(송사리)의 행동변화와 관련된 분자생물학적 기전 규명을 통하여 비정상적 행동의 모니터링을 위한 생물지표(biomarker)를 개발하는데 있다. 이를 위해 우선 suppression subtractive hybridization (SSH) 및 DNA microarray 기법을 활용하여 다양한 유전자를 스크리닝하였다. 다이아지논에 노출시킨 송사리에서 발현의 차이가 나는 상향 조절된 유전자 97개 (알려지지 않은 유전자 27개 포함)와 하향 조절된 유전자 99개 (알려지지 않은 유전자 60개 포함)를 동정 하였고 이들 중 이상행동과 관련되는 것으로 보이는 유전자 10개 (상향조절 5개, 하향조절 5개)를 선발하였다. 이들 중에서 primer 제작이 잘된 beta-1, Orla C3-1, parvalbumin 및 apolipoprotein E을 선발하여 그 유전자 발현을 real-time PCR 기법을 사용하여 정량적으로 모니터링 하였다. Orla C3-1, parvalbumin 및 apolipoprotein E는 고농도의 다이아지논 처리 (1000 ppb; 24 h)에서 그 발현이 억제됨이 관찰되었다. 다이아지논 처리 시 신경질환 (알츠하이머 병 및 다운 신드롬)에 관련된 apolipoprotein E와 근육세포의 유연화에 작용하는 parvalbumin 등의 발현억제는 송사리의 인지능력 교란 및 근육세포의 경직 등을 각각 유도하여 송사리의 비정상적 행동을 야기하는 것으로 판단되었다. 따라서 이들 생물지표는 신경독성물질에 의한 송사리 및 기타 어류의 이상행동의 변화의 감지에 활용될 수 있을 것으로 사료된다.

황산염 환원 박테리아에 의한 열처리강에 대한 미생물부식의 전기화학적 분석

고성철,신성규,박근현,문경만 대한환경공학회 2002 대한환경공학회 학술발표논문집 Vol.2002 No.12