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제7차 교육과정에 따른 산ㆍ염기 단원의 STS과학 교육에 적합한 CBL 실험 프로그램의 개발
이광필,김동화,최성호,류재정,이무상 慶北大學校 師範大學 科學敎育硏究所 2001 科學敎育硏究誌 Vol.25 No.-
This study developed the 'advanced-supplementary' course experimental program with CBL(Calculator-Based Laboratory) system in conformity with the reform of the 7th science curriculum and applied the CBL experimental program to 'acid-base' chapter of middle-high school. The results of this study shows that a practical use of CBL system as powerful instrument can be applied to experiment program of 'acid-base' chapter in order to causes learner's motivation and enlarge a sphere of inquiry-activity in science.
Helicobacter pylori 편모 유전자의 클로닝 및 염기서열 분석
이광호,이우곤,조명제,도영미,백승철,강경희,박필성,이상룡 경상대학교 유전공학연구소 1993 遺傳工學硏究所報 Vol.12 No.-
A λgt11 expression libary of H. pylori DNA in E. coli Y1090 was screened with flagellin-specific rabbit antiserum for molecular cloning of the flagellin gene of H. pylori. A positive clone, λHPF4, was obtained and the recombinant antigen expressed from λHPF4 was a fusion protein with the molecular weight of 168kd. Sequence analysis of antigen-encoding DNA showed that an open reading frame composed of 1,536 nucleotides encodes a polypepride with a oredicted molecular size of 54kd. This open reading frame did not show the homology with flaA gene encoding 56kd protein of H. pylori and was confirmed as a unique sequence through homoligy searching. Therefore, the cloned antigen is supposed to be the carboxy-terminal region of the other flagellin protein of H. pylori, flaB, with the molecular weight of 58kd.
치과 치료 경험이 아동의 치과의사와 치과 치료에 대한 태도에 끼치는 영향에 관한 조사 연구
허용욱,이광희,문필성,김대업 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.2
The purpose of this study was to investgated the effect of dental experience the attitude of children toward the dentist and the dental treatment.The subject of the study were 1,090 children from second grade to forth grade of three elementary schools of the Iksan city.The children were investgated by the questionnaire which cosisted of seven items about the attitude toward the dentist,the attitude toward the dental treatment,the dental treatment experience,the number of times of dental treatment,the last time of the dental visit,pain of dental treatment,and fear of dental treatment. There were significant differences among the grades in the attitude toward the dental treatment and in the attitude toward the dentist(P<0.01).Second grade children had the most negative attitudes.But,there were no differences between boys and girls.Girls felt more pain and fear than boys(P<0.01).There were significant relationships between the pain and the attitude toward the dentist,between the pain and the attitude toward the dentist, and between the fear and the attitude toward the dental treatment(P<0.01,all).The number of dental visit had significant relationship with the attitude toward the dental treatment(P<0.05).There was significant relationship between the pain and the fear between the attitude toward the dentist and the attitude toward the dental treatmen.
The Study of Doxorubicin and its Complex with DNA by SERS and UV-resonance Raman Spectroscopy
Lee, Chul-Jae,Kang, Jae-Soo,Kim, Mak-Soon,Lee, Kwang-Pill,Lee, Mu-Sang Korean Chemical Society 2004 Bulletin of the Korean Chemical Society Vol.25 No.8
The interaction of the antitumour agent doxorubicin with calf thymus DNA is investigated in an aqueous solution at a pH level of 6-7 with molar ratios of 1/10. A UV-resonance Raman spectroscopy and surface enhanced Raman spectroscopy are used to determine the doxorubicin binding sites and the structural variations of doxorubicin-DNA complexes in an aqueous solution. Doxorubicin intercalates with adenine and guanine via a hydrogen bond formation between the N7 positions of purine bases and the hydroxyl group of doxorubicin.
Lee, Kwang-Pill,Gopalan, Anantha Iyengar,Lee, Se Hee,Kim, Min Seok IOP Pub 2006 Nanotechnology Vol.17 No.2
<P>Aqueous dispersions of chiral polyaniline nanobundles (C-PANI-NB) were prepared by simple interfacial polymerization using immiscible organic/aqueous biphasic systems. C-PANI-NB arise from the intertwining of PANI chains over interconnected units of cyclodextrin sulfate. Formation of C-PANI-NB was witnessed only in the aqueous phase, irrespective of the organic solvent used in the interfacial polymerization. The PANI that formed in the aqueous phase existed in the C-PANI-NB form. This was evident from field emission transmission microscopy. The chiro-optical properties of C-PANI-NB are strikingly different from those of simple chiral polyaniline. C-PANI-NB exhibit size selective enantioseparation. C-PANI-NB are expected to find applications as modifiers in capillary electrophoresis, as catalysts for size selective asymmetric synthesis and in biological sensors. Also, the method adopted here for the preparation of C-PANI-NB could be extended to other conducting polymers. </P>
Lee Chang-Hyun,Cho Hwang-Yoon,Kil Kwang-Soo,Lee Gun-Soup,Yoon Ji-Yeon,Lee Young-Jae,Kim Eun-Young,Park Se-Pill,Lim Jin-Ho 한국발생생물학회 2003 한국발생생물학회 학술발표대회 Vol.2003 No.1
The purpose of this study is to evaluate an efficacy of in vitro differentiated human embryonic stem (hES, MB03) cells expressing Nurr1 in relief of symptomatic motor behavior of Parkinson's disease (PD) animal models MB03 was genetically modified to express Nurr1 protein and was induced to differentiate according to 2-/4+ protocol using retinoic acid and ascorbic acid. The differentiation-induced cells were selected for 10 to 20 days thereafter in N2 medium. Upon selection, cells expressing GFAP, TH, or NF200 were 38.8%, 11%, and 20.5%, respectively. in order to examine therapeutic effects of the differentiated cells in PD animal model, rats were unilaterally lesioned by administration of 6-kydroxydopamine HCI (6-OHDA) into medial forebrain region (MFB, AP -4.4 mm, ML 1.2 mm, DV 78 mm with incision bar set at -2.4 mm), as a reference to bregma and the surface of the skull. Confirmation of successful lesion by apomorphine-induced rotational behavior, differentiated cells were transplanted into the striatum (AP 1.0, ML 3.5, DV -5.0; AP 0.6, ML 2.5, DV -4.5). Improvements of asymmetric motor behavior by the transplantation were examined every two weeks after the surgery. In two weeks, numbers of rotation by the experimental rats were (P<0.05) of the number before transplantation, however, the ratio increased slightly to in six weeks. In contrast, the ratio of sham-grafted animals ranged from 112.3+8.5% to 139.2+28.9% during the examination. Immunohistochemical studies further confirmed the presence, survival, migration, and expression of TH of the transplanted human cells.
Lee, Kwang-Pill,Manesh, Kalayil Manian,Kim, Kyu Soo,Gopalan, Anantha Iyengar American Scientific Publishers 2009 Journal of Nanoscience and Nanotechnology Vol.9 No.1
<P>Using the combined techniques of sol-gel and electrospinning, nanostructures (wire (1D) to plates (3D)) of LiV3O8 are prepared with poly vinyl alcohol (PVA) as the template. A precursor gel of LiOH-V2O5 is prepared in alkaline medium. Fibrous membranes of PVA with different weight% of LiOH-V2O5 gel are prepared by electrospinning and designated as PVA/LiOH-V2O5 composite membrane fibers. The composite fibrous membranes are subjected to calcination at 500 degrees C to obtain different nanostructured LiV3O8. FESEM images of LiV3O8 inform that as the loading % of LiOH-V2O5 gel in the composite membrane increased, the morphology of LiV3O8 changed from fibrous wires (1D) to plates (3D). FESEM images reveal that the diameters of the composite fibrous membranes, PVA/LiOH-V2O5 are in the range of 80-120 nm. Results from X-ray diffraction and thermal studies clearly confirm the formation of LiV3O8 after calcination of PVA/LiOH-V2O5 composite membrane at 500 degrees C. It is presumed that this preparation strategy can be extended to obtain nanostructures of other inorganic oxides.</P>
Fabrication and properties of poly(vinylidenefluoride)/PbS/Au heterogeneous nanostructures.
Lee, Kwang-Pill,Gopalan, Anantha Iyengar,Park, Jong Wook,Ragupathy, Dhanusuraman,Manesh, Kalayil Manian American Scientific Publishers 2009 Journal of Nanoscience and Nanotechnology Vol.9 No.1
<P>We report on the fabrication of polyvinylidenefluoride (PVdF) PVdF/PbS and PVdF/PbS/Au heterogeneous nanostructures by the processes, electrospinning and chemical treatment. Initially electrospinning a solution consisting of PVdF and lead acetate was used to form PVdF nanofibers loaded with Pb ions. Exposure of Pb ions loaded PVdF fibers to H2S resulted in PVdF/PbS nanostructures. The deposition of gold nanoparticles onto PVdF/PbS nanostructures results in PVdF/PbS/Au heterogeneous structure. The existence of PbS particles with an average diameter of 11 nm is evident from field emission transmission electron microscopy (FETEM) image of PVdF/PbS. The results from X-ray diffraction of PVdF/PbS also predict the size of PbS particles as in accordance with FETEM. A blue shift in the optical transition of PbS is noticed in the UV-visible spectrum of PVdF/PbS as a result of quantum confinement effect. The band gap of PbS is influenced by the presence of Au nanoparticles over the PbS particles. An equal atomic weight % of Au and PbS is found in the PVdF/PbS/Au nanostructure as inferred from energy dispersive X-ray spectroscopy (EDX). Photoluminescence (PL) spectra of PVdF/PbS and PVdF/PbS/Au are compared. Emission peaks are noticed at 400 nm and 480 nm for PVdF/PbS and PVdF/PbS/Au nanostructures respectively for an excitation wavelength of 254 nm. The presence of Au nanoclusters in PVdF/PbS/Au diminishes the intensity of photo emission of PbS.</P>