생쥐에 있어서 單一害球가 分離된 受精卵의 移植에 의한 새끼生産에 관한 硏究

崔有林,全益秀,金宣宜,崔光洙,朴修奉,辛明宰 慶北大學校農業科學技術硏究所 1991 慶北大農學誌 Vol.9 No.-

本 연구는 생쥐의 4細胞期胚에서 하나의 割球를 뽑아내는 새로운 割球分離技術인 biopsy에 대한 효율성과 分離된 受精卵과 分離한 割球의 생존성 및 새끼생산율을 검토한 結果이다. 그 結果를 要約하면 다음과 같다. 1. 4細胞期胚에서 分離한 單一割球와 4細胞期胚를 M2 배양액에 배양한 結果, 單一割球는 82.6%가 영양배엽을 형성한 割球로 發生하였고 4細胞期胚로 89.5%가 胚盤胞期胚로 發生하였다. 2. 4細胞期胚를 biopsy하여 하나의 割球가 分離된 受精卵과 대조구인 4細胞期胚를 M2배양액에서 배양한 결과 각각 83.3%와 90.4%가 胚盤細胞胚로 發生하였다. 3. Biopsy하여 4細胞期胚에서 分離한 單一割球와 대조구인 4細胞期胚에서 4개의 割球로 분리한 單一割球를 M2 배양액에서 배양한 결과 각각 80.8%와 83.3%가 영양배엽을 형성한 割球로 發生하였다. 4. 4細胞期胚에서 하나의 割球를 뽑아낸 受精卵과 대조구인 4細胞期胚를 수란생쥐에 이식한 결과 각각 36.0%와 48.6%의 새끼쥐 생산率을 얻었다. The study was carried out to investigate the viability and the offspring production rate of single blastomeres and biopsied 4-cell mouse embryos and, also to examine the efficiency of biopsy. The results obtained are summerised as follows : 1. The separated blastomeres from 4-cell embryos and intact 4-cell embryos which were cultured in Medium 2 were developed to trophoblastic vesicle and blastocyst embryo by 82.6% and 89.5%, respectively. 2. The biopsied embryos from 4-cell embryos and intact 4-cell embryos which cultured in Medium 2 were developed to blastocyst embryo by 83.3% and 90.4%, respectively. 3. The biospied blastomeres and the separated blastomeres from 4-cell embryos which were cultured in Medium 2 were developed to trophoblastic vesicle by 80.8% and 83.3%, respectively. 4. The biopsied embryos from 4-cell embryos and intact 4-cell embryos were transferred to recipients, and the offspring rate was 36% and 48.6%, respectively.

프레탈^(R)정(실로스타졸 50mg)에 대한 실로졸^(R)정의 생물학적 동등성

최한곤,권기철,이승호,김학미,박병주,유봉규,이종달,이경희,하정희,우종수,박인숙,최진석,용철순 한국병원약사회 2003 병원약사회지 Vol.20 No.1

Bioequivalence of two cilostazol tablets, the Pletaal^(R)(Korea Otsuka Pharmaceutical Co., Ltd.) and the Cilozol^(R)(Hanmi Pharmaceutical Co., Ltd.), was evaluated according to the guideline of KFDA, Sixteen normal male volunteers(age 20~29 years old) were divided into two groups and a randomized 22 cross-over study was employed. After two tablets containing 50㎎ of cilostazol were orally administered. blood was taken at predetermined time intervals and the concentration of cilostazol in plasma was determined with an HPLC method using UV detector. The pharmacokinetic parameters(C_(max), T_(max) and AUC_(t)) were calculated and ANOVA was utilized for the statistical analysis of parameters. The results showed that the differences in C_(max), T_(max) and AUC_(t) between two tablets were 4.99%, 1.74% and 7.68%, respectively. The powers(1-β) for C_(max), T_(max) and AUC_(t) were83.92%, 80.12% and 85.03%, respectively. Detectable differences(Δ) and confidence intervals were all less than 20%, and confidence interval of all the parameters were also less than 20% at the significance level(α) of 0.05. All of these parameters met the criteria of KFDA for bioequivalence, indicating that Cilozol^(R) tablet is bioequivalent to Pletaal^(R) tablet.

하청 근로자들의 건강수준 평가

최홍열,고상백,장세진,차봉석,임형준,이상윤,김재용,강동묵,조수헌 大韓産業醫學會 2001 대한직업환경의학회지 Vol.13 No.1

목 적 : 하청노동을 이용한 노동 유연화 전략은 근로자들의 고용을 불안정시키고, 근로자들의 삶에 부정적 영향을 미치고 있다. 따라서 이 연구는 하청업체 근로자들의 고용현황을 조사하고, 기존의 업체중심의 건강평가의 문제점을 지적하고자 하였다. 또한 하청업체 근로자들의 건강평가의 현재적 의미와 사회적 배제의 특성을 알아보고자 하였다. 방 법 : 모기업과 하청업체 근로자들의 건강수준을 비교하기 위하여, 정기건강진단(일반, 특수)과 채용건강진단 자료를 이용하고자 하였다. 건강진단에서 누락된 비정규직을 포괄하기 위하여 SF-36을 이용하여 건강설문조사를 시행하였다.결 과 : 조선업 하청업체 근로자들의 고용기간은 짧고, 빈번한 노동이동 현상을 보였다. 3년간 건강진단 수검 현황을 통해 살펴볼 때, 1998년 수검자중 2000년까지 연속해서 검진 받은 수검자는 40.3%였다. 건강진단 결과 유소견자 규모를 통해 모기업과 하청업체간의 건강수준을 비교해 보았다. 모기업은 일반질병 요관찰자와 질병자를 포함한 유소견자가 1,011명으로 약 10 %의 유소견율을 보이고 있고, 질병자의 경우 596명으로 유병률이 5 %를 상회하고 있다. 반면 하청업체의 경우 1,967명의 수검자중 유소견자가 115명으로 6 %의 유소견율을 보이고있고, 질병자는 82명으로 4 %의 유병률을 보이고있다 채용건강진단을 받은 수검자는 6,260명 이었으며, 이 중 유소견자 수는 2,373명으로 전체 수검자의 37.9 %를 차지하였다. 건강설문지의 경우는 전체 건강수준의 총합은 하청보다 원청 근로자가 높았고 통계적으로 유의하였다. 결 론' 정기건강검진 결과는 하청근로자들이 고용형태에 따라 검진을 받지 못한 경우가 많아, 건강 근로자 효과로 표현될 가능성이 있다. 따라서 채용건강진단 결과는 다른 측면에서 하청업체 근로자들의 건강수준의 현실적 반영이라고 할 수 있다. 향후 하청업체 근로자들의 건강문제를 감시하고 개선하려면 기존의 업체 중심의 접근으로는 하청 근로자의 건강문제를 제대로 다룰 수 없고, 업종과 지역을 동시에 고려하여 접근할 필요가 있다. 또한 단기 고용 근로자 및 비정규직 근로자를 사회보장의 확충과 모기업의 연대책임 강화 및 기업복지 차원에서 포괄하는 방안이 모색되어야 하겠다. Objectives : The strategy for the labor flexibility through subcontracted labor have brought a negative effect on the lives of the workers, such as the increase of the unstable employment. This study was conducted to analyze the relationship between the employment of subcontracted firms and the health status of them, and to find out some problems which have been caused in the process of health evaluation and the characteristics of social exclusion. Methods : We reviewed the periodical health examination and preemployment health examination in order to compare health status of the subcontracted workers with those of the parent firm workers. The SF-36 questionnaire was administered to assess the self-perceived health status. Results : The result shows that compared to the working duration of the parent firm workers, those of the subcontracted workers were shorter, and the job transfer rate was higher. In the periodical health examination, the prevalence of occupational and general disease was higher in the parent firm(10%) than in the subcontracted firms(6%). Preemployment health examination plays an important role in worker's health manage- ment. Among 6,260 workers who received preemployment health examination, the prevalence of disease was 37.9%. Mean scores of health status measured by SF-36 were lower in the subcontracted firm than in the parent firm. Conclusions : Due to social exclusion of the subcontracted workers from the periodical health examination, there is a possibility that the periodical health examination have the healthy worker effect. These results suggests that the preemployment health examination could be a more appropriate indicator which is able to assess the health status of the subcontracted workers than the periodical health examination.

제2형 당뇨병 환자에서의 지속적 피하 인슐린 주입 치료시 관해

최의광,최수봉,박선민 건국대학교 의과학연구소 2001 건국의과학학술지 Vol.11 No.-

Background: It is well known that chronic hyperglycemia can deteriorate pancreatic beta cell function and insulin sensitivity in animal and human studies, and the normalization of blood glucose can reverse them. Our purpose of the study was to investigate the remission pattern after treating patients with continuous subcutaneous insulin infusion (CSII) therapy, and compare the clinical characteristics of remitted and non-remitted patients in a retrospective way. Methods: We selected 91 type 2 diabetic patients who had been admitted to KonKuk University hospital from January 1993 to August 1993. After hospitalization, all subjects were treated with insulin pump (Sooil, Seoul, Korea) for intensive blood glucose control until remission occurred. Remission is defined as long-term good glycemic control without any medication. After fifteen to seventeen months of CSII therapy, the induction of remission was examined. Serum c-peptide levels, hemoglobin A1c, and serum lipid profiles were measured after hospitalization and before discharge. Results: Overall 34.4% of all subjects had remission after 53.6±38.9 days of CSII therapy and remission lasted for average 13.6 ± 8.9 months during the study periods. Some clinical characteristics of the patients prior to CSII therapy influenced the remission occurrence. The remission was more frequently induced when patients started CSII therapy with insulin pump in younger age, higher body mass index, shorter diabetic duration, lower post-prandial blood glucose levels, higher post-pandial serum c-peptide levels, and less chronic diabetic complications. Conculsion: These findings suggest that long-term CSII therapy can induce remission in a significant proportion of mild type 2 diabetic patients. Thus, it is desirable that the intensive insulin treatment by CSII is considered as not the last treatment., but an initial management of mild type 2 diabetic patients.

플라즈마 용사법을 이용한 세라믹 코팅된 ICBT SPINDLE DISK 개발

최순돈,민봉기,최경수 영남대학교 공업기술연구소 2001 工業技術硏究所論文集 Vol.29 No.1

The plasma spray technics has known as one of the surface modification methods to improve the mechanical properties or the functional charactristics of materials. This paper has been aimed to investigate the effects of plasma sprayed conditions, such as spray distance and arc power level, of plasma sprayed Al2O3-40%TiO2 costing layer. The optimum processing for ICBT spindle disk coated plasma spray are showed at spray distance 80mm, APL 40kw.

Fe-N薄膜의 磁氣特性 및 結晶構造 解析

崔奉洙 大田工業專門大學 1982 論文集 Vol.31 No.-

The author has made the thin films which are deposited Fe in the pressure of nitrogen gas (2×10^-^5torr ~ 9×10^-^3torr), and investigated the effect of residual ags on the magnetic properties, its annealing effect, and crystal structure of these films according to the function of the pressure. Then, the results of the experiment are as the following: 1. The Fe thin films are deposited in low partial pressure of nitrogen gas (2×10^-^5torr). In this case, the saturation magnetization (Ms), the coercive force (Hc) and the crystal structure are 1720 gauss, 60 Oe and b.c.c. 2. The Fe thin films are deposited in a low partial pressure of nitrogen gas (2×10^-^4torr). In this case, the Ms, the Hc and the crystal structure are 1940 gauss, 155 Oe and mixed with b.c.c. and f.c.c. The Ms, in a low partial pressure of nitrogen gas (4×10^-^4torr ~ 1×10^-^3torr) has the high value more than 1900 gauss. That is why Fe_16N_2 is generated. 3. The iron films which are in a low partial pressure of nitrogen gas (1×10^-^3torr ~ 9×10^-^3torr) show the rapid reduction of Ms, and the increase of Hc. 4. After annealing at more than 300℃, in the Fe thin films which are deposited in the pressure of nitrogen gas (2×10^-^5torr ~ 9×10^-^3torr), the Ms decreased, and the Hc increased evidently. This result can be explained as the increase of Fe-N compound (γ^1-Fe_4N).

당뇨병에서 지속적 피하인슐린주입(Continuous Subcutaneous Insulin Infusion) 치료법의 임상 적용

최수봉 건국대학교 의과학연구소 1995 건국의과학학술지 Vol.5 No.-

From the experience of treating diabetic patients by continuous subcutaneous insulin infusion during past 12 years, the clinical aspects of continuous subcutaneous insulin infusion therapy in the general hospital setting are summarized. For selection of patients, motivation for the normal glucose control, responsibility, intelligence and financial support ability are considered. All patients are hospitalized and educated intensively about the basic knowledge of diabetes mellitus, home blood glucose monitoring, handling technique of insulin pump, exercise and diet therapy. The blood glucose levels fall from 363.1±42.0(mean SD)㎎/㎗ to 128.7±18.6㎎/㎗ after two weeks' treatment of continuous subcutaneous insulin infusion. Glycosylated hemoglobin levels fall from 10.82±2.52% to 6.01±1.68% after three months' treatment. Eighty-six percent of patients experience well-being sensation after five days' continuous subcutaneous insulin infusion treatment. Twenty-eight percent of patients are in remission state after 1 year's continuous subcutaneous insulin infusion treatment. It is concluded that the continuous subcutaneous insulin infusion treatment is effective for the normalization of blood glucose, the prevention of diabetic complications, and good health maintenance for selected diabetic patients.

대구지역 대학생의 치아우식 활성과 타액성분에 대한 연구

최수영,민봉희 대구대학교 기초과학연구소 2000 基礎科學硏究 Vol.16 No.2

This study was performed to evaluate the relationship between dental caries and salivary components or properties. Twenty two university students were selected randomly and their salivary factors including viscosity, pH, buffering capacity, and microbiological distribution were analyzed. Among whole case of 22, three students(16.7%) had a moderate activity of dental caries and the rest were caries-free as a result of laboratory tests. The viscosity, acid formation, and the distribution of microorganisms in positive students for dental caries were higher than those in negative students but buffering capacity was low relatively in carious students. In microbiological investigations, there was no significant difference in total bacterial counts between carious and caries-free students. For the standardization and elucidation of dental caries factors, many microorganisms were isolated from carious teeth and identified by using commercial identification kits. Most of the isolated strains belonged to the Genus Streptococcus and identified as Streptococcus agalacoae, Enterococcus faecium and Enterococcus avium by the physiological and biochemical characterization methods. However, the causative agent of dental caries, Streptococcus mutans was not isolated in this study.

窒化鐵 薄膜의 電氣抵抗

崔奉洙 충남대학교 대학원 1976 論文集 Vol.6 No.-

An apparatus for measuring resistivity of thin films was made, and the measurement of resistivity has been performed satisfactorily. The apparatus consists of placing four probes that make contact along a line on the surface of the sample. Current is passed through the outer pair of probes and the flouting potential is measured across the inner pair. The experimental results by use of this four point probe are compared with some magnetic properties. The author has identified the remarkable tendency in the experiments of measurement of resistivity, and obtained the results as follows, 1) The resistivities of nitrided iron films which were made in a low partial pressure of nitrogen gas (ie, 10^(-5)~10^(-4) Torr) shows the increasement depending upon the lattice defection 2) The resistivities of those samples which were made in a middle partial pressure of nitrogen gas (ie, 10^(-4)~10^(-3) Torr) shows almost like constant value. This result can be explain as a formation of uniform Fe-N films. 3) The resistivities of those samples which were made in a high partial pressure(ie, 10^(-3)~10^(-2) Torr) shows the increasing curve caused by the amount of impurity atoms. 4) The resistivities of samples after annealing at 400℃ decreased as a half of the original samples. This effect is thougt to originate with invasion of nitrogen atoms, which located at grain boundaries as an impurity, into the iron lattice and formation of Fe-N type compounds.

부신 제거 백서에 티타늄 임플랜트 매식후 주위 골형성에 관한 연구

최갑림,김종렬,박봉수,성일용 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.3

The use of dental implants has increased tremendously in recent years and is expected to increase even more in the future. The successful outcome of any implant procedure is surely dependent on interrelationship of the various components of an equation that includes biocompatibility of implant material, macroscopic and microscopic nature of the implant surface, the status of implant bed, surgical technique, undisturbed healing phase and subsequent prosthetic design and long-term loading phase. The purpose of this study was to clarify the effects of adrenalectomy on the osseointegration of pure titanium implants. Seventy rats, 11 weeks of age, were divided into two groups : an adrenalectomized group and a control group. Titanium screw implant(diameter, 2.0mm ; length, 3.5mm) was placed into left tibia of 70 rats, 35 in control group and 35 in the experimental group. The rats were sacrificed at different time interval (1, 2, 3, 4, 6, 8, and 12 weeks after implantation) for histopathologic observation, histomorphometric analysis and immunohistochemistry with fibronectin and CD44 antibody. The results obtained from this study were as follows : 1.Histopathogically, findings, newly formed bone was seen at 3 weeks control group and became lamellar bone at 12 weeks. At 6 weeks, lipocytes were observed in bone marrow space. Thickness of regenerated trabecular bone increased till 6 weeks after then, that decreased gradually. 2.By histomorphometric analysis, marrow bone density and contact ratio of marrow bone to implant decreased significantly from 8 to 12 weeks in experimental group compared to control group and also total bone to implant contact ratio decreased significantly from 4 to 12 weeks in experimental group compared to control group. 3.Fibronectin immunoreactivity was very strong at 3 and 4 weeks control group. And after that reduced gradually. But it was continuously strong from 1 to 12 weeks experimental group. 4.CD44 immunoreactivity was very strong in the newly formed osteoblasts at 3 and 4 weeks control group. But it reacted minimally later. However, it reacted continuously strong from 3 to 12 weeks experimental group. From these results, bone to implant contact ratio decreased gradually from 4 weeks in adrenalectomized group compared to control group. CD44 and fibronectin immunoreactivities were strong at all times in adrenalectomized rats. Therefore, it could be stated that immature bone remained continuously for a long time and not readily proceeded into mature status.