Protective Effects of Hyperoside against Carbon Tetrachloride-Induced Liver Damage in Mice

Choi, Jun-Ho,Kim, Dong-Wook,Yun, Nari,Choi, Jae-Sue,Islam, Md. Nurul,Kim, Yeong-Shik,Lee, Sun-Mee American Chemical Society and American Society of 2011 Journal of natural products Vol.74 No.5

<P>In this study, the hepatoprotective effects of hyperoside (<B>1</B>), a flavonoid glycoside isolated from <I>Artemisia capillaris</I>, have been examined against carbon tetrachloride (CCl<SUB>4</SUB>)-induced liver injury. Mice were treated intraperitoneally with vehicle or <B>1</B> (50, 100, and 200 mg·kg<SUP>−1</SUP>) 30 min before and 2 h after CCl<SUB>4</SUB> (20 μL·kg<SUP>−1</SUP>) injection. Levels of serum aminotransferases were increased 24 h after CCl<SUB>4</SUB> injection, and these increases were attenuated by <B>1</B>. Histological analysis showed that <B>1</B> prevented portal inflammation, centrizonal necrosis, and Kupffer cell hyperplasia. Lipid peroxidation was increased and hepatic glutathione content was decreased significantly after CCl<SUB>4</SUB> treatment, and these changes were reduced by administration of <B>1</B>. Protein and mRNA expression of tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and heme oxygenase-1 (HO-1) and nuclear protein expression of nuclear factor erythroid 2-related factor 2 (Nrf2) significantly increased after CCl<SUB>4</SUB> injection. Compound <B>1</B> suppressed TNF-α, iNOS, and COX-2 protein and mRNA expression and augmented HO-1 protein and mRNA expression and Nrf2 nuclear protein expression. These results suggest that <B>1</B> has protective effects against CCl<SUB>4</SUB>-induced acute liver injury, and this protection is likely due to enhancement of the antioxidative defense system and suppression of the inflammatory response.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2011/jnprdf.2011.74.issue-5/np200001x/production/images/medium/np-2011-00001x_0006.gif'></P>

7두의 개에서 배설성 요로조영술을 통한 신장 기능 평가

최윤정,이기자,최형준,이용진,박성준,송근호,정성목,최호정,이영원 忠南大學校 獸醫科大學 動物醫科學硏究所 2005 動物醫科學硏究誌 Vol.13 No.-

Excretory urography is a type of contrast study used to verify and localize upper urinary tract disease. It is an inexpensive and easy way to visualize of anatomic and functional status of the kidney and has been used as a primary imaging modality for the evaluation of urinary tract abnormalities. We describe urological signs of 7 dogs who examined with excretory urography and ultrasonography. Six cases were referred to veterinary medical teaching hospital. Chungnam national university (VMTH, CNU) to evaluate renal function after the treatment for renal failure and one case was referred showing hematuria. In case 1, 2 and 4, blood test and urinalysis was normal and the results of excretory urography presented that renal function were normal range. In case 3, the results of urinalysis, ultrasonography, and excretory urography except blood test presented abnormal kidney and hydronephrosis was diagnosed. In case 5 and 6, blood test, urinalysis, ultrasonography and excretory urography indicated renal failure. In case 7, blood test, urinalysis and ultrasonography presented partially abnormal signs, however, the results of excretory urography was normal range.

혈액투석 환자의 가성 고칼륨혈증

최창렬,조형도,강태영,이준수,한호,정청일,이동규,유준호,한상웅,김호중 대한신장학회 2001 대한신장학회잡지 Vol.20 No.5

단기간의 금연과 일회성 유산소 운동이 흡연자의 심혈관 반응에 미치는 영향

송준경(Jun Kyung·choi),최현민(Hyun Min Choi),김종경(Jong Kyung Kim),노호성(Ho Sung Nho) 한국사회체육학회 2012 한국사회체육학회지 Vol.0 No.48

The purpose of this study was to investigate cardiovascular responses to a bout of aerobic exercise and acute smoking cessation in smoker. nine collegiate male smoker who have been smoking(duration of smoking: 7.5 ± 0.9yrs; number of cigarettes per day: 15.5 ± 1.5) participated in this study. All subjects performed PEI to stimulate Group IV metaboreceptor at immediately after smoking, 24hours after smoking cessation and smoking after a bout of aerobic exercise. they were measured SBP, DBP, HR, SV, CO, TVC using by finometer(FMS co, Netherland). As a result, compared to immediately after smoking, SBP, DBP, and MAP were significantly decreased 24-hours after smoking cessation at rest and PEI(p<0.05). In addition, smoking after a bout of aerobic exercise caused by post-exercise hypotension indicated that SBP was significantly decreased compared to immediately after smoking(p<0.05). also, the changes in DBP and MAP at rest and handgrip exercise were significantly smaller compared to immediately after smoking(p<0.05). Thus, the results showed that smoking cessation during 24-hours and a bout of aerobic exercise relieve excess blood pressure response and enhanced arterial baroreflex in collegiate male smoker. It is concluded that caused exercise pressor reflex by stimulated Group IV may be effective to improve excess blood pressure responses.

간 이식 환자에서 발생한 파종성 Scedosporium apiospermum 감염 1예

박현구,최성호,강호석,송주형,정지훈,소민욱,최상호,김양수,우준희 대한감염학회 2006 감염과 화학요법 Vol.38 No.5

저자들은 다발성 뇌 농양을 동반한 치명적인 파종성 S. apiospermum 감염이 발생한 간 이식 환자를 경험하였다. 본 증례의 환자는 voriconazole로 치료하는 호전경과를 보이다가 진균성 뇌 동맥류 파열로 사망하였다. S. apio-spermum은 비교적 드문 진균으로 병리학적으로 Aspergillus spp.와 매우 유사하고 amphotericin B에 내성을 나타내므로 진단과 치료가 적절하게 이루어지지 못할 가능성이 많고 면역 기능 저하 환자에서 혈행성 전파를 통한 파종성 감염이 용이하게 발생하므로 높은 사망률을 나타낸다. 이식 환자를 비롯한 면역 기능 저하 환자에서 진균감염이 의심될 때 S. apiospermum과 같은 드문 진균에 의한 감염증도 감별진단에 포함해야 하겠다. Sedosporium apiospermum is a saprophytic fungus commonly found in soil and polluted water. This organism is known as a cause of mycetoma, which may occur in immunocompetent hosts following trauma. However, in immunocompromised patients, S. apiospermum can also cause life-threatening invasive disease, including central nervous system infection or disseminated infection. We report a fatal case of disseminated S. apiospermum infection in a 46-year-old woman after liver transplantation. Eight days postoperatively, she developed pneumonia, followed by altered mentality in the 15 days. A head CT demonstrated multiple brain abscesses. Sputum and stereotactic-aspirated brain abscess culture yielded S. apiospermum. Despite treatment with voriconazole, the patient died of intracranial hemorrhage.

기저질환이 없는 사람에서 발생한 Campylobacter fetus에 의한 재발성 심내막염 1예

전재범,정진원,김성혜,송문희,오형철,안성기,최상호,김남중,김양수,우준희,류지소 대한감염학회 2004 감염과 화학요법 Vol.36 No.6

C. fetus 심내막염은 드물게 발생하는 질환으로 주로 면역능이 저하된 환자에서 발병하고 대동맥판을 침범하는 경우가 많으며 적절한 치료가 되지 않을 경우 치사율이 높다. 저자들은 기저질환이 없던 69세 남자에서 C. fetus 심내막염을 경험하였기에 문헌고찰과 함께 보고하는 바이다. 환자는 발열로 내원하여 시행한 혈액배양 검사상 C. fetus가 동정되었고 경식도 초음파 검사상 삼첨판에 증식증이 발견되어 심내막염 진단하에 ceftriaxone에 이어 meropenem으로 총 4주간 항생제를 투여받고 호전되었다가 한달 후 다시 재발하였고 판막부전 소견 진행하여 삼첨판 치환술을 시행받고 전신상태 호전되어 퇴원하였다. Campylobacter fetus is a rare cause of endocarditis. To date, only about 30 cases of C. fetus endocarditis have been reported mainly in the elderly with chronic underlying illnesses. Most cases showed tendency to involve aortic valve. This report describes a case of native tricuspid valve infective endocarditis in a 69-year-old man without any underlying disease. Despite adequate antibiotic therapy for 4-weeks, endocarditis recurred and leading to surgical valve replacement.

<i>Toxoplasma gondii</i> protects against H₂O₂‐induced apoptosis in ARPE‐19 cells through the transcriptional regulation of apoptotic elements and downregulation of the p38 MAPK pathway

Choi, Si‐,Hwan,Park, Sung Jun,Cha, Guang‐,Ho,Quan, Juan Hua,Chang, Nam‐,Sik,Ahn, Myoung‐,Hee,Shin, Dae‐,Whan,Lee, Young‐,Ha Blackwell Publishing Ltd 2011 Acta ophthalmologica Vol.89 No.4

<P><B>Abstract.</B></P><P><B>Purpose: </B> Toxoplasmosis, which is caused by the protozoan parasite <I>Toxoplasma gondii</I>, can lead to severe visual impairment. <I>T.?gondii</I> inhibits or delays programmed cell death caused by various apoptotic triggers; however, the mechanisms involved in the <I>T</I>.?<I>gondii</I>‐induced suppression of apoptosis in retinal cells have not been analysed in detail.</P><P><B>Methods: </B> We investigated the role of <I>T</I>.<I>?gondii</I> infection in H<SUB>2</SUB>O<SUB>2</SUB>‐induced apoptosis in human retinal pigment epithelial cells (ARPE‐19) by monitoring the activities of apoptosis‐regulating molecules and mitogen‐activated protein kinases (MAPKs), including p38 MAPK. We also examined the gene downstream from p38 MAPK.</P><P><B>Results: </B> <I>T.?gondii</I> infection significantly inhibited the cellular toxicity of H<SUB>2</SUB>O<SUB>2</SUB> (500 μ<SMALL>m</SMALL>) and increased cell viability in a multiplicity of infection (MOI)‐dependent manner by reducing DNA fragmentation and reactive oxygen species (ROS) generation in ARPE‐19 cells. Western blot analysis also showed that <I>T</I>.?<I>gondii</I> infection prevented the host cell expression of pro‐apoptotic factors, such as Bad and Bax, and the activation of caspase‐3. Infection with <I>T</I>.?<I>gondii</I> increased the expression of the anti‐apoptotic factor Bcl‐2 in ARPE‐19 cells under oxidative stress. In accordance with these findings, <I>Toxoplasma</I> infection was protective enough to suppress the phosphorylation of p38 MAPK following H<SUB>2</SUB>O<SUB>2</SUB> treatment. Exposure to H<SUB>2</SUB>O<SUB>2</SUB> increased the expression of heme oxygenase‐1 (HO‐1) in ARPE‐19 cells, and its expression was significantly inhibited in H<SUB>2</SUB>O<SUB>2</SUB>‐treated infected cells.</P><P><B>Conclusion: </B> The protective function of <I>T</I>.?<I>gondii</I> infection against ROS‐induced apoptosis results from changes in the expression of apoptotic molecules and the downregulation of stress‐induced intracellular signalling.</P>

중국ㆍASEAN FTA에 따른 관세인하의 영향과 시사점

최준호,홍승린 한국관세학회 2007 관세학회지 Vol.8 No.1

This paper inspects beforehand China, ASEAN FTA begun substantially according to China, ASEAN sign trade in services agreement between FTA, and customs brokers who is taking charge of 97% of import and export customs clearance of our country wishes to investigate about segment to correspond hereafter according to role enlargement with China and ASEAN. Main result of this paper is as following. First, will have to grasp change circumstance strictly market environment that follow to China-ASEAN FTA as for our enterprise, and propel market extension for these area at the same time through strategic investment about China-ASEAN. Second, role which registered customs brokers of our country prepare FTA and first of all, are important with China-ASEAN is achievement of consulting business by FTA conclusion including certificate of origin. Third, Our government should prepare Korea-ASEAN ETA for early tariff reduction realization of ASEAN interest item.

복합 치아종에 관한 증례보고

최형준,최병재,손흥규,양호정 大韓小兒齒科學會 1995 大韓小兒齒科學會誌 Vol.22 No.2

The odontoma is relatively a common odontogenic tumor. It has come to mean as a growth in which both the epithelial and the mesenchymal cell exhibit complete differentiation, with the result that functional ameloblast and odontoblast form enamel and dentin. The etiology of it is unknown, but it has been suggested that local trauma or infection may lead to the production of such a lesion. Odontma is divided into 2 types. One is compound odontoma that is at least superficial anatomic simiarity to normal teeth, and the other is complex odontoma, that calcified dental tissues are simply an irregular mass bearing no morphologic similarity even to rudimentary teeth. Somtimes this malformation is not easily identified because its lacking of calcification. So, if permanant tooth is delayed to erupt, one should take a careful look the radiographic film to find that whether the cause of it is odontoma or not. In this case report, 3 patients with odontoma who visited Dept. of Pediatric Dentistry in Yonsei Dental College, were treated by means of surgical removal. The obtained results were as follows: 1. The odontoma is usually related to impacted tooth, and it is occurred neighboring to the crown of impacted tooth that have normally developed. So, in the case of missing tooth and delayed eruption, its presence should be investigated. 2. The odontoma can be seen vaguely radiographically, so, it is difficulty to make diagnosis. 3. The treatment of odontoma is a surgical removal. And, if it is completely removed, its occurrence is rare. 4. In the case of impacted tooth being associated with compound odontoma, continuous management is necessary for the eruption of impacted tooth after the surgical extraction of odontoma. 5. It resembles the ameloblastic odontoma radigraphically, and if it is the case, the treatment may vary, so the confirmation through histologic examination after removal of mass is necessary.

방사선학적 치근단병소의 크기와 특징에 대한 치근단낭종 및 육아종의 상관관계연구

최호식,이우철,손원준,금기연,배광식,백승호 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.1

The purpose of this study was to find out the relationship of radiographic lesion size, gender, age of patients and radiographic character to the diagnosis of periapical cyst and granuloma. The data was collected from 187 periapical lesions of 167 patients who undergone apical surgery at Department of Conservative Dentistry, Seoul National University Dental Hospital from 2003 to 2005. The lesion were surgically removed and send for biopsy to the Oral Pathology Laboratory. From the initial radiograph, lesion size was calculated using PiViweSTAR (INFINITT, Korea) program. The obtained data were statistically evaluated using SPSS (p<0.05). The result were as followings: 1. From 187 biopsy samples, the incidence of periapical cyst was 28.34% and granuloma was 65.24%. 2. There was a significant correlation between periapical cyst and the size of radiographic lesion (p<0.01). 3. There were no significant correlations between age, gender, location of lesion and the final diagnosis (p>0.05). 4. There was a significant correlation between the non-demarcation of the lesion and the incidence of periapical granuloma (p<0.01). 본 연구는 2003년부터 2005년까지 서울대학교 치과병원 보존과에 내원한 환자 중 치근단수술을 시행한 환자 167명에서 생검을 위해 적출한 187개의 치근단병소를 대상으로 하였다. 수술 후 조직검사 결과 얻은 진단명과 환자의 진단기록에서 환자의 성별, 나이, 발병 부위, 방사선 사진 상의 특징 그리고 병소의 크기와의 상관관계를 조사하였다. 초진시 방사선 사진에서 치근단병소의 크기를 PiViewSTAR (INFINITT, Korea)를 이용하여 화소를 계산함으로써 면적을 구하였다. 이를 바탕으로 통계 분석프로그램 SPSS (version 12.0K, SPSS Inc., Chicago, IL, USA)를 이용해 방사선사진상 크기와 진단명과의 상관관계를 조사하였다. 나이와 진단명과의 상관관계는 일원배치 분산분석을 시행하고 성별, 부위, 방사선사진상의 특징과 진단명과의 상관관계는 교차분석을 통해 카이제곱검정으로 조사하여 다음과 같은 결론을 얻었다. 1. 187의 치근단병소 조직검사결과 치근단병소 중 치근단낭종의 비율은 28.34%, 육아종의 비율은 65.24%, 기타병소의 비율은 6.42%로 나타났다. 2. 방사선사진상 병소의 크기가 커질수록 치근단 낭종일 확률이 높았다(p<0.01). 3. 나이, 성별, 발병부위와 진단명사이에서 유의 한 상관관계가 없었다(p>0.05). 4. 병소의 경계가 명확하지 않은 것과 치근단육아종의 발생빈도는 통계적으로 유의한 상관관계를 보였다(p<0.01).