감초 추출물이 면역 응답에 미치는 영향

심호기,박무희,최청,배만종 한국식품영양학회 1997 韓國食品營養學會誌 Vol.10 No.4

감초에서 분리한 각각의 추출물(GHE, GME, GAE)을 BALB/c mice에 투여하여 탐식작용, 용혈 반형성, 용혈소 역가 측정 및 rosette 형성 실험을 통해 감초가 면역응답에 미치는 영향을 조사하였다. 1. 탐식능 측정에서 복강침출세포(PEC)와 비장세포(spleen cells)는 CON군에 비하여 각각의 약물투여군이 Candida parapsilosis에 대한 효과가 다소 높은 경향을 나타내었지만, 유의성은 없었다. 2. 용혈반 형성 및 용혈소 역가측정에서는 CON군에 비하여 GHE, GME 투여군 및 GAE 투여 군이 높게 나타났다. 3. Rosette형성에 대한 측정에서는 CON군에 비하여 GME, GAE 투여군이 높은 유의성을 나타내었다. This study was conduced to investigate on immune response of the hot water extract(PHE), 50% methanol extract(PME) and acetone extract(PAE) from Glycyrrhiza glabra. The experiment was carried out by phagocytosis, plaque forming cell(PEC), hemolysin titration(HY) and rosette forming cell(RFC) assay by using BALB/c mice. The results obtained from this study are as follow ; The effects of Glycyrrhiza glabra extracts on phygocytosis was tended to beslight increase in GME and GAE groups compared to the control group, but not significant. In the experiment of PFC and HY, the results of experiment groups which was given each samples were significantly higher than the control group. The result of rosette forming cell in GME and GAE groups were significantly higher than control group.

Agrocybe cylindracea로부터 추출한 다당류의 면역증강활성

김선희,이항우,배만종,이재성 한국미생물학회 2000 미생물학회지 Vol.36 No.1

Agrocybe cylindracea으로부터 분리 배양한 균사체 배양액을 이용하여 균사체내 다당류(IP)와 균사체외 다당류(EP)를 분리하고, 동물실험을 통한 각 다당류의 면역증강활성에 관해 실험을 실시하였다. 탐식능에 대한 실험결과, IP와 EP 모두는 복강 침출 세포(PEC)에서 대조군에 비해 6.6-50%, 말초 임파구(ML)에서 67-167%, 비장(SP)에서 16-90%의 전반적으로 높은 탐식능을 나타냈다. 또한 항체 생성능 측정을 위한 plaque forming cell(PFC)와 rosette forming cell(RFC)의 실험결과에서 볼 때, PFC형성능은 대조군에 비해 EP투여 II군에서 46-50%, IP투여 I군에서 49-70%의 활성증가를 나타내었다. 또한 RFC형성능은 대조군에 비해 IP투여 I군에서 91%의 증가를, EP투여 I군에서 33%, EP투여 II군에서 43%의 활성증가를 나타내어 높은 항체생성이 기대되었다. Mitogen 활성은 농도 의존적으로 세포 증식능을 보였으며, IP와 EP를 비교할 때, EP는 IP에 비해 약2.5배의 세포증식 활성을 보였다. The immunopotentiating effects of the polysaccharides, both intracellular and extracellular, was examined by an animal feeding test. The results are summarized as follows. The oral administration of intracellular and extracellular polysaccharides of Agrocybe cylindracea for 10 days resulted in the enhanced phagocytic activity of peritoneal exudate cells(PEC), spleen cells(SC), and monolymphocytes(ML). In the experiment of PFC(plaque forming cell) and RFC(rosette forming cell), the results showed that all the polysaccharide fractions enhanced the immune related cells. The EAC II group(the extracellular polysaccharide of Agrocybe cylindracea 10 mg/0.2 ml distilled water/day/ mouse) increased the PFC and RFC by 46~50% and 43%, respectively, compared to the control group. On the other hand, the IAC I group(the intracellular polysaccharide of Agrocybe cylindracea 1 mg/0.2 ml distilled water/day/mouse) increased the PFC and RFC by 49~70% and 91%, respectively. In terms of the mitogenic activity, the extracellular polysaccharides of A. cylindracea showed a higher activity than the intracellular polysaccharides.

동물성 소염진통제(I) -구인다당체분획의 소염,진통 및 면역억제작용-

김창종(Chang Johng Kim),최윤석(Yun Seuk Choi),조승길(Seung Kil Cho) 대한약학회 1991 약학회지 Vol.35 No.2

Effects of Allolobophora caliginosatrapezoides (Ac) polysaccharide fractions on the inflammation and hypersensitivity were studied in vivo. It showed that Ac polysaccharide fractions have the significant inhibitory activities of inflammation and hypersensitivity; They inhibited significantly the carrageenin-induced paw edema and acetic acid-induced writhing syndrome. They also inhibited significantly the Arthus reaction and delayed hypersensitivity in the sheep red blood cell-sensitized mice in accordance with the inhibition of haemaglutinin titer, haemolysin titer, plaque-forming cells and rosette-forming cells. They also improved markedly the oxazolone-induced dermatitis in rats dose-dependently. As the above results, it exhibited that Ac polysaccharide fraction inhibited not only humoral immune response, but also cell-mediated immune response. It seemed that methanol and ether extracts have also another physiological active agents.

香砂平胃散이 抗癌 및 免疫調節作用에 미치는 影響

하지용,조영호,이지숙 대한한방종양학회 2000 대한한방종양학회지 Vol.6 No.1

In order to investigate annumor and immune response effect by Hyangsapyungwisan after Sarcoma- 180 cells and methotrexate were treared each other. the extract of Hyangsapyungwisan was orally administered to ICR mice for 14 days. To evaluate the effects of the Hyangsapyungwisan, 50% inhibition concentration(IC_50), mean survival days, tumor weight for antitumor effects, hemagglutinin titer, hemolysin titer, rosette forming cells, natural killer cell and productivity of interleukin-2 for immune responses measured in ICR mice. The results were summarized as follows: 1. Mean survival time in Hyangsapyungwisan-treated group was slightly prolonged, as compared with control group(13.46%). 2. On the MTT assay, cell viability was significantly inhibited by 5ug/well, 2.5ug/well, l.25ug/well. and 0.625ug/well of Hyangsapyung-wism concentration inhibited cell viability significantly. IC_5o for cell viability was 11.59ug/well. 3. Tumor weight in Hyangsapyungwisan treated group was depressed, as compared with the control group(P<0.05). 4. Hemagglutinin titer in Hyangsapyungwisan-treated group was slightly increased with no significance, as compared with the control group. 5. Hemolysin titer in Hyangsapyungwisan-treated group was silightly increased, as compared with the control group(P<0.05). 6. Rosette forming cells in Hyangsapyungwisan-treated group was silightly increased, as compared with the control group(P<0.05). 7. Naural killer cell activity in Hyangsapyungwisan-treated group was significantly increased(P<0.05). 8. Production of interleukin-2 was significantly increased(P<0.05). According to the above results, Hyangsapygwisan had prominent antitumor, and enhance both cellular and humoral immunity in mice.

향사평위산(香砂平胃散)이 항암(抗癌) 및 면역조절작용(免疫調節作用)에 미치는 영향(影響)

하지용,조영호,이지숙,Ha, Jee-Yong,Jo, Young-Ho,Lee, Jee-Suk 대한암한의학회 2000 大韓癌韓醫學會誌 Vol.6 No.1

In order to investigate antitumor and immune response effect by Hyangsapyungwisan after Sarcoma-180 cells and methotrexate were treatred each other, the extract of Hyangsapyungwisan was orally administered to ICR mice for 14 days. To evaluate the effects of the Hyangsapyungwisan, 50% inhibition concentration($IC_{50}$), mean survival days, tumor weight for antitumor effects, hemagglutinin titer, hemolysin titer, rosette forming cells, natural killer cell activity and productivity of interleukin-2 for immune responses measured in ICR mice. The results were summarized as follows: 1. Mean survival time in Hyangsapyungwisan-treated group was slightly prolonged, as compared with control group(13.46%). 2. On the MTT assay, cell viability was significantly inhibited by $5{\mu}g/well,\;2.5{\mu}g/well,\;1.25{\mu}g/well,\;and\;0.625{\mu}g/well$ of Hyangsapyung-wisan concentration inhibited cell viability significantly. $IC_{50}$ for cell viability was $11.59{\mu}g/well$. 3. Tumor weight in Hyangsapyungwisan treated group was depressed, as compared with the control group(p<0.05). 4. Hemagglutinin titer in Hyangsapyungwisan-treated group was slightly increased with no significance, as compared with the control group. 5. Hemolysin titer in Hyangsapyungwisan-treated group was silightly increased, as compared with the control group(p<0.05). 6. Rosette forming cells in Hyangsapyungwisan-treated group was silightly increased, as compared with the control group(p<0.05). 7. Naural killer cell activity in Hyangsapyungwisan-treated group was significantly increased(p<0.05). 8. Production of interleukin-2 was significantly increased(p<0.05). According to the above results, Hyangsapygwisan had prominent antitumor effects, and enhance both cellular and humoral immunity in mice.

감잎(Diospyros kaki folium)의 Polyphenol 화합물군이 면역기능에 미치는 영향

배만종,성태수,최청,안봉전,박무희 경산대학교 생명자원개발연구소 1999 생명자원과 산업 Vol.3 No.-

한국산 감잎(Diospyros kaki folium)으로부터 polyphenol 화합물을 분리하여, 감잎의 기능성 식품으로서의 근거를 제시하고자, 면역기능의 활성화에 관한 실험을 행한 결과 다음과 같은 결과를 얻었다. 감잎의 폴리페놀 화합물이 면역기능에 미치는 영향에 관한 실험에서는 감잎으로부터 분리된 폴리페놀 화합물 Ⅰ, Ⅱ 및 Ⅲ에 비하여 시료를 투여한 군에서 항체생산이 증가되었으며, 특히 폴리페놀 화합물 Ⅱ와 Ⅲ에서 높은 유의성을 나타내었다. Rosette 형성의 결과에서도 감잎의 폴리페놀 화합물 Ⅱ와 Ⅲ을 투여한 군에서 분리된 비장과 복강 상재성 마크로파지에서 rosette 형성능이 높은 것으로 나타났다. Candida에 대한 시료의 탐식능을 관찰한 결과, 대조군에 비하여 fraction Ⅰ은 70%, Ⅱ는 76.8% 및 Ⅲ은 93%의 탐식능의 효과를 나타내었다. 또한, 면역기능을 담당하는 기관인 비장에 관한 세포증식능을 실험한 결과 대조군에 비하여 시료를 투여한 모든 군에서 비장세포가 증가하였으며, 시료의 농도가 증가할수록 세포증식도 증가되는 경향을 나타내었다. This study was conducted to investigate immuno functional activity of the ployphenol fractions isolated from persimmom leaves(Diospyros kaki folum). In the experiment of rosette forming cell, the results showed that all the polyphenol fractions enhanced the cell count compared with the control group. Especially polyphenol fraction Ⅱ and Ⅲ showed highly significant effect. The phagocytic activity of peritoneal macrophage on mice was significantly increased by the polyphenol fractions of the persimmon leaves compared with that of the control group. The gagocytic activity of polyphenol fraction Ⅰ, Ⅱ and Ⅲ oagainst the C. parapsilosis was 26.4%, 72% and 82.3% respectively. The pagocytic activity of the same fractions against the C. albicans was 70%, 76.8% and 93% respectively.

Count of T-rosette-forming cells (T-RFC) in the Spleen of Young Rats Born to Azathioprind(Imuran)-administered Pregnant Rats

Lee, Wang Jae,Shin, Chan Soo,Chang, Ka Young,Lee, Kwang Ho Seoul National University 1987 The seoul journal of medicine Vol.28 No.1

한국산 배의 Polyphenol 화합물군이 쥐의 면역기능에 미치는 영향

최희진,한호석,박정혜,배종호,우희섭,안봉전,배만종,김현구,최청 한국식생활문화학회 2003 韓國食生活文化學會誌 Vol.18 No.4

This study was conducted to investigate immunofunctional activity of the polyphenol fractions isolated from Korean pear. In the experimental of Rosette forming cell, the results showed that all the polyphenol fractions enhance the cell count compared with the control group. Especially polyphenol fraction II and III showed highly significant effect on Rosette forming cell, and allergy inhibition. After antigen challenge, histamine content of blood of the polyphenol groups was lowered to near the normal group.

의이인(薏苡仁)의 투여(投與)가 마우스의 세포성(細胞性) 및 체액성(體液性) 면역기능(免疫機能)에 미치는 영향(影響)

우영은,김형균,송봉근,이언정,Woo, Young-Eun,Kim, Hyeong-Kyun,Song, Bong-Keun,Lee, Eon-Jeong 한국한의학연구원 1996 한국 한의학연구소 논문집 Vol.2 No.1

Coicis Semen is one of the oriental medicine that has been used for the treatment of the diseases such as pulmonary abscess, periappendicular abscess and wart since ancient times. However, the mechanism of the action of the drug is not well studied. This study was done to investigate the effects of Coicis Semen on the host defence mechanism. Effects of Coicis Semen on the immune responses were analysed by measuring the contact hypersensitivity, hemagglutinin, hemolysin and rosette formation, cytotoxicity, and reactive oxygen intermidiates production. As the results, water extract of Coicis Semen administration enhanced the antibodies (hemagglutinin and hemolysin) formation and the appearance of rosette forming cells of the spleen. Also Coicis Semen increased the allogeneic immune response in the mouse, showed cytotoxic activity against human leukemia cell line(K562) and decreased the contact hypersensitivity against dinitroflurobenzene. Also administration of Coicis Senlen slightly increased NK cell activity and enhanced the production of such reactive oxygen intermediates as superoxide and hydrogen peroxide from the macrophages in vivo and in vitro. The above results demonstrate that Coicis Semen has enhancing effects on cellular and humoral immune responses against disease.

薏苡仁의 投與가 마우스의 細胞性 및 體液性 免疫機能에 미치는 影響

우영은,김형균,송봉근,이언정 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1

Coicis Semen is one of the oriental medicine that has been used for the treatment of the disease such as pulmonary abscess, periappendicular abscess and wart since ancient times. However, the mechanism of the action of the drug is not well studied. This study was done to investigate the effects of Coicis Semen on the host defense mechanism. Effects of Coicis Semen on the immune responses were analyzed by measuring the contact gypersensitivity, hemagglutinin, hemolysin and rosette formation, cytotoxicity, and reactive oxygen intermidiates production. As the results, water extract of Coicis Semen administration enhanced the antibodies (hemagglutinin and hemolysin) formation and the appearance of rosette forming cells of the spleen. Also Coicis Semen increased the allogeneic immune response in the mouse, showed cytotoxic activity against human leukemia cell line(K562) and decreased the contact hypersensitivity against dinitroflurobenzene. Also administration of Coicis Semen slightly increased NK cell activity and enhanced the production of such reactive oxygen intermidiates as superoxide and hydrogen peroxide from the macrophages in vivo and in vitro. The above results demonstrate that Coicis Semen has enhancing effects on cellular and humoral immune responses against disease.