붉은 장미꽃잎 에탄올 추출물의 활성 분획물 분석 및 항염증 활성 효과

김현경 국제문화기술진흥원 2020 The Journal of the Convergence on Culture Technolo Vol.6 No.2

The purpose of this study was to investigate the effects of red rose petal is an abundant source of phenolics and is traditionally used as a food supplement and as herbal medicine. Various plant phenolics are known to have anticancer, antioxidant, and anti-inflammatory properties. In this study, we investigated the anti-inflammatory effects of red rose ethanolic extracts(GRP) from red rose petal in lipopolysaccharide (LPS)-activated RAW 264.7 cells. Our results demonstrated that pretreatment of GRP(500 μg/mL) significantly reduced NO production by suppressing iNOS protein expression in LPS-stimulated cells. Anti-inflammatory effects byred rose petal were observed in the following. red rose petal inhibited the translocation of NF-κB from the cytosol to the nucleus via the suppression of IκB-α phosphorylation and also inhibited LPS-stimulated NF-κB transcriptional activity. These findings suggest that red rose petal exert anti-inflammatory actions and help to elucidate the mechanisms underlying the potential therapeutic values of red rose petal. Therefore, red rose petal could be regarded as a potential source of natural anti-inflammatory agents. 붉은 장미꽃잎 에탄올 추출물은 효과가 풍부한 페놀 성분의 공급원이며 전통적으로 식품 보충제 및 약초로 사용되고 있다. 또한 다양한 식물페놀 성분은 항암, 항산화 및 항염증 특성을 갖는 것으로 알려져 있다. 따라서 본 연구에서는 붉은 장미꽃잎 에탄올추출물(GRP) 유래 활성 분획물 분석 및 lipopolysaccharide(LPS)로 유도된 마우스 대식세포주인 RAW264.7 세포와 마우스 폐포 대식세포주인 MH-S 세포에서 붉은 장미꽃잎 에탄올추출물의 항염증 활성 효과를 조사하였다. 그 결과 GRP(500 μg/ mL)의 전처리 LPS 자극 세포에서 iNOS 단백질 및 염증성 싸이토카인 mRNA 발현을 크게 억제하였다. 빨간 장미꽃잎 에탄올 추출물에 의한 항균활성 효과는 다음과 같이 관찰되었다. 붉은 장미꽃잎은 IκB-α 인산화의 억제를 통해 시토졸에서 핵으로의 NF-κB의 전좌를 억제하고 또한 LPS로 자극된 NF-κB 전사 활성을 억제 하였다. 이러한 연구 결과는 빨간 장미꽃잎 에탄올 추출물이 항염증 작용을 발휘 하고 빨간 장미꽃잎의 잠재적인 치료 가치성 및 기본 메커니즘을 규명 할 수 있었다. 결론적으로 붉은 장미꽃잎 에탄올 추출물이 다양한 활성 성분 함유 및 천연 항염증제의 잠재적 공급원으로 활용 가능성이 있음을 시사 하였다.

The Anti-Inflammatory and Anti-Oxidant Activity of Ethanol Extract from Red Rose Petals

Kim, Hyun-Kyoung The Institute of Internet 2020 International Journal of Internet, Broadcasting an Vol.12 No.3

Red rose petals are usually disposed but they are an abundant source of phenolics and traditionally used as food supplement and as herbal medicine. Of the Various phenolics, they are known to have anticancer, antioxidant, and anti-inflammatory properties. In this study, we investigated the anti-inflammatory effects of red rose ethanolic extracts (GRP) on lipopolysaccharide (LPS)-activated RAW 264.7 cells. The results demonstrated that pretreatment of GRP (500㎍/mL) significantly reduced NO production by suppressing iNOS protein expression in LPS-stimulated cells. Anti-inflammatory effects by red rose petals were observed in the following. Red rose petals inhibited the translocation of NF-κB from the cytosol to the nucleus via the suppression of IκB-α phosphorylation and also inhibited LPS-stimulated NF-κB transcriptional activity. These findings suggest that red rose petals exert anti-inflammatory actions and help to elucidate the mechanisms underlying the potential therapeutic values of red rose petals. Therefore, red rose petals could be regarded as a potential source of natural anti-inflammatory agents.

국내 육성 장미 품종 꽃잎 유래 체세포배 발생 캘러스 유도

이수영,신주영,이영아,안창호,김예진,박필만,안혜련,이가연,정현환,Lee, Su Young,Shin, Ju Young,Lee, Young Ah,Ahn, Chang Ho,Kim, Yae Jin,Park, Pil Man,An, Hye Ryun,Lee, Ka Youn,Jung, Hyun Hwan 한국자원식물학회 2022 한국자원식물학회지 Vol.35 No.5

This study was conducted to induce somatic embryogenic callus (SEC) derived from petals in rose. The petal explants of 3 cultivars ('Ice Wing', 'Orange Eye' and 'Pink Beauty') with different flower colors were placed on three types media (MS, SH and WPM) supplemented with 11 mg/L 2,4-D, respectively, and then cultured in the dark for 47 days. Calluses were formed at explants of all three cultivars. Also, 'Ice Wing', which were cultured in the SH as the basal medium, showed the highest callus formation rate. However, somatic embryos were generated from only petal-derived callus of 'Ice Wing', which were induced on the WPM as the basal medium, transferred it to SH basal medium supplemented with 3 mg/L 2,4-D, and 300 mg/L L-proline, and cultured for 5 weeks. The SEC has been proliferated every four weeks at the subculture interval. In addition, as a results of making a comparison of expression of RhSERK3 and RhSERK4, which is used as signal for generation of somatic embryo from callus in rose, between the SEC and petal-derived callus from 'Ice Wing' by RT-qPCR, the former showed 10 times higher RhSERK3 expression and 700 times higher RhSERK4 expression than the latter.

꽃잎 수가 많은 복숭아색 스탠다드 절화 장미 ‘투디오스’ 육성

허문선(Moon-Sun Heo),황수경(Soo-Kyung Hwang),윤재수(Jae-Soo Yoon),강병철(Byoung-Cheorl Kang) 한국원예학회 2016 원예과학기술지 Vol.34 No.5

A standard rose cultivar, ‘To Dios’(Rosa hybrida) was selected for use as a cut flower from the progeny of a cross between ‘Vanilla perfume’ and ‘Marcia’ at the research and development division of the Goyang-si Agricultural Technology Center in 2013. ‘Vanilla perfume’, an orange-colored (RHS Orange Group 27C) standard rose cultivar with 48 petals, was used as the female plant. ‘Marcia’, a white-colored (RHS Green white Group 157B) standard rose cultivar with 96 petals was used as the male parent. A cross was made in 2009 and seedlings were produced. Selections were made between 2010 and 2013, and a plant with good cut flower traits was finally selected and named ‘To Dios’. ‘To Dios’ is a standard rose with large flowers of 11.2 ㎝ in diameter and 128 peach-colored (RHS Red Group 36B) petals per flower. Vase life of this cultivar is up to 15 days. It takes 47 days from pruning to blooming and cut flower productivity is approximately 160 stems/㎡ per year. ‘To Dios’ was registered as a new cultivar No. 4875 with the Korea Seed & Variety Service on March 19, 2014.

Effects of Red and White Rose Petal Extracts and Ganoderma lucidum Culture on Ovalbumin-Induced Atopic Dermatitis

Jeong Hee Jeon,Sang-Chul Kwon,Dongsun Park,Sunhee Shin,Min-Jung Jang,Seong Soo Joo,Hyomin Kang,Sung Hyun Kim,Jee Young Oh,Jae-Hyun Jeong,Yun-Bae Kim 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.3

The purpose of this study was to evaluate the efficacy of red rose petal extract (RRPE), white rose petal extract (WRPE) and Ganoderma lucidium culture (GLC) in a mouse model of atopic dermatitis induced by ovalbumin (OVA). Female BALB/c mice were intraperitoneally sensitized with OVA (10 ㎍) plus aluminum hydroxide (alum, 4 ㎎) three times weekly and, after 1 week, dermally challenged with patches containing OVA (100 ㎍) for 2 weeks. RRPE, WRPE and GLC were administrated intraperitoneally or orally once a day from day 0 to 35. Intraperitoneal administration of WRPE (5 or 20 ㎎/㎏) inhibited dermal lesions such as erythema/hemorrhage, edema, excoriation and dryness. In particular, oral administration of WRPE (300 ㎎/㎏) markedly attenuated the symptoms as well as epidermal hyperplasia and infiltration of inflammatory cells including mast cells in dermis. In addition, WRPE significantly decreased serum IgE, histamine and interleukin-4 (IL-4) levels. In comparison, despite of inhibitory effects of RRPE and GLC on serum IgE, histamine and/or IL-4 levels, these compounds did not attenuate both the skin symptoms and histopathological findings. Taken together, it is suggested that WRPE could be a potential candidate for the attenuation of chronic atopic dermatitis.

Extraction conditions of white rose petals for the inhibition of enzymes related to skin aging

최은경,궈하이유,최재권,장수길,신경하,차예슬,최용진,서다움,이윤복,주성수,김윤배 한국실험동물학회 2015 Laboratory Animal Research Vol.31 No.3

In order to assess inhibitory potentials of white rose petal extracts (WRPE) on the activities of enzymes related to dermal aging according to the extraction conditions, three extraction methods were adopted. WRPE was prepared by extracting dried white rose (Rosa hybrida) petals with 50% ethanol (WRPE-EtOH), Pectinex® SMASH XXL enzyme (WRPE-enzyme) or high temperature-high pressure (WRPE-HTHP). In the inhibition of matrix metalloproteinase-1, although the enzyme activity was fully inhibited by all 3 extracts at 100 μg/mL in 60 min, partial inhibition (50-70%) was achieved only by WRPE-EtOH and WRPE-enzyme at 50 μg/mL. High concentrations (≥250 μg/mL) of all 3 extracts markedly inhibited the elastase activity. However, at low concentrations (15.6-125 μg/mL), only WRPE-EtOH inhibited the enzyme activity. Notably, WRPE-EtOH was superior to WRPE-enzyme and WRPE-HTHP in the inhibition of tyrosinase. WRPE-EtOH significantly inhibited the enzyme activity from 31.2 μM, reaching 80% inhibition at 125 μM. In addition to its strong antioxidative activity, the ethanol extract of white rose petals was confirmed to be effective in inhibiting skin aging-related enzymes. Therefore, it is suggested that WRPE-EtOH could be a good candidate for the improvement of skin aging such as wrinkle formation and pigmentation.

Original Article: Biochemistry/Molecular Biology : Extraction Conditions for Phenolic Compounds with Antioxidant Activities from White Rose Petals

( Jae Kwon Choi ),( Yoon Bok Lee ),( Kyun Hee Lee ),( Hae Cheon Im ),( Yun Bae Kim ),( Ehn Kyoung Choi ),( Seong Soo Joo ),( Su Kil Jang ),( Nam Soo Han ),( Chung Ho Kim ) 한국응용생명화학회 2015 Journal of Applied Biological Chemistry (J. Appl. Vol.58 No.2

The extract of white rose petals has an antioxidant effect and can be used to treat allergic disease. The purpose of this study was to identify optimal conditions for extracting antioxidative compounds from white rose petals with 2,2-diphenyl-1- picrylhydrazyl scavenging activities. A response surface methodology based on a central composite design was used to investigate the effects of three independent variables: ethanol concentration (X1), extraction temperature (X2), and extraction time (X3). The estimated optimal conditions for obtaining phenolic compounds with antioxidant activities were as follows: ethanol concentration of 42% (X1), extraction time of 80 min (X3), and extraction temperature of 75oC (X2). The estimated optimal conditions for obtaining flavonoid compounds with antioxidant effects were an ethanol concentration of 41% (X1), extraction time of 119 min (X3), and an extraction temperature of 75oC (X2). Under these conditions, predicted response values for the phenolic and flavonoid contents were 243.5 mg gallic acid equivalent/g dry mass and 19.93 mg catechin equivalent (CE)/g dry mass, respectively.

Rose Petal Extract Ameliorates Obesity in High Fat Diet-Induced Obese Mice

Jaeeun Jung,Minhee Lee,Seong-Hoo Park,Wonhee Cho,Jinhak Kim,Sangwon Eun,Jeongmin Lee 한국식품영양과학회 2024 Preventive Nutrition and Food Science Vol.29 No.2

In Asia, Rosa spp. has been used in traditional medicine for the treatment of osteoarthritis, rheumatoid arthritis, and edema. In this study, we investigated the effect of rose petal extract (RPE) on high fat diet (HFD)-induced obesity in mice. C57BL/6J mice were fed with either an AIN-93G diet (normal control), a 60% HFD, or a HFD plus supplementation with RPE at 100 or 200 mg/kg body weight (HFD+R100, HFD+R200) for 14 weeks. The HFD increased the body weight gain, liver and fat weight, lipid profiles (total cholesterol, triglyceride, high density lipoprotein cholesterol, and low density lipoprotein cholesterol), and the serum aspartate aminotransferase and alanine aminotransferase levels of mice, while RPE supplementation significantly decreased these parameters compared with the HFD group. Furthermore, the HFD increased the protein expressions of adipogenesis- and lipogenesis-related factors and decreased the protein expression of lipolysis- and energy metabolism-related factors. Conversely, RPE supplementation significantly decreased the protein expression of adipogenesis- and lipogenesis-related factors and increased the protein expression of lipolysis- and energy metabolism-related factors compared to the HFD group. Taken together, the results provide preliminary evidence for the potential protective effects of the RPE against obesity.

Anti-allergic Effects of Rose Petal Extract and Ganoderma lucidum Culture on Mast Cell-Mediated Allergy Model

Sang-Chul Kwon,Sunhee Shin,Jeong Hee Jeon,Dongsun Park,Min-Jung Jang,Jwa Jin Kim,Chang Hwan Kim,Jae-Hyun Jeong,Yun-Bae Kim 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.1

The effects of rose petal extract (RPE) and Ganoderma lucidum culture (GLC) on the systemic and local anaphylactic reactions induced by compound 48/80 were investigated in mice. To induce systemic anaphylactic shock, male ICR mice were intraperitoneally challenged with a lethal dose (8 ㎎/㎏) of compound 48/80, a mast cell degranulator, 30 min after intraperitoneal administration of RPE or GLC (10, 32 or 100 ㎎/㎏). In comparison with 100% death, within 60 min, of control mice administered with vehicle, the mortalities of mice pretreated with 32 and 100 ㎎/㎏ of RPE were decreased to 20 and 0%, respectively. A dose-dependent decrease in the mortality was also achieved with GLC, resulting in 60, 20 and 0% in mice pretreated with 10, 32 and 100 ㎎/㎏, respectively. In local atopic dermatitis induced by intradermal injection of compound 48/80 (50 ㎍/site), the mice exhibited 169.1±10.5 times of scratching behaviors for 30 min and vascular leakage of Evan's blue; 118.3±16.9 mm2 in size and 2.61±0.48 μg in amount. The scratching behaviors induced by compound 48/80 was significantly attenuated by 6-day oral pretreatment with RPE (300 ㎎/㎏) to 86.9±16.4 times. In addition, Evan's blue leakage was also greatly reduced by RPE. In contrast, GLC did not decrease both the number of scratching behaviors and dye leakage, implying that different factors other than histamine are also involved in the atopic dermatitis induced by compound 48/80. Taken together, it is suggested that RPE contains ingredients inhibiting degranulation or action of inflammatory mediators from mast cells, and that could be a promising candidate for the prevention of systemic or local allergic reactions including atopic dermatitis.

품종별 장미꽃 메탄올 추출물의 항염증 효과

이선미,이림,성지혜,양진우,김영화,정헌상,이준수,Lee, Seon-Mi,Li, Lin,Sung, Jeehye,Yang, Jinwoo,Kim, Younghwa,Jeong, Heon Sang,Lee, Junsoo 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.4

본 연구에서는 장미꽃 methanol 추출물의 항염증 활성을 조사하기 위하여 LPS에 의해 염증이 유도된 RAW 264.7 대식세포에서 염증억제 효과를 알아보았다. 염증 억제의 지표로서는 세포가 방출하는 NO 생성량과 iNOS 및 $NF-{\kappa}B$ 발현 정도를 측정하였다. 실험 결과, RAW 264.7 대식세포에 대한 품종별 장미꽃 methanol 추출물($500{\mu}g/mL$)이 NO의 함량을 감소시키는 경향을 나타내었다. NO의 생성에 영향을 미치는 iNOS 단백질의 발현량을 측정한 결과, LPS 처리에 의해 활성화된 iNOS 단백질의 발현이 장미꽃 methanol 추출물 처리 시 유의적으로 수준으로 억제하는 경향을 보였다. Luciferase activity를 실행한 결과, LPS로 자극한 세포와 비교하였을 때 염증과 관련된 $NF-{\kappa}B$ promoter activity가 장미꽃 methanol 추출물 처리시 현저히 감소하는 경향을 나타내었고, 세포질의 $I{\kappa}B{\alpha}$의 인산화를 저해함으로써 전사요소인 $NF-{\kappa}B$ p65, p50을 핵 속으로 유리시키는 과정을 억제하였다. 이 결과로 장미꽃 methanol 추출물이 전사단계에서 저해활성을 나타낸다는 것을 확인하였다. 본 연구결과, 장미꽃 methanol 추출물은 항염증 효과를 나타냄에 따라 만성 질환 예방을 위한 기능성 식품의 원료로 활용될 수 있을 것으로 여겨진다. The genus Rosa (Rosaceae) is an abundant source of phenolics and is traditionally used as a food supplement and as herbal medicine. Various plant phenolics are known to have anticancer, antioxidant, and anti-inflammatory properties. In this study, we investigated the anti-inflammatory effects of rose methanolic extracts (RMEs) from four different rose cultivars (Macarena, Onnuri, Oklahoma, and Colorado) in lipopolysaccharide (LPS)-activated RAW 264.7 cells. Our results demonstrated that pretreatment of REMs ($500{\mu}g/mL$) significantly reduced NO production by suppressing iNOS protein expression in LPS-stimulated cells. Anti-inflammatory effects by RMEs were observed in the following order: Oklahoma > Colorado > Onnuri > Macarena. Consistent with this finding, RMEs inhibited the translocation of $NF-{\kappa}B$ from the cytosol to the nucleus via the suppression of $I{\kappa}B{\alpha}$ phosphorylation and also inhibited LPS-stimulated $NF-{\kappa}B$ transcriptional activity. These findings suggest that RMEs exert anti-inflammatory actions and help to elucidate the mechanisms underlying the potential therapeutic values of RMEs. Therefore, RMEs could be regarded as a potential source of natural anti-inflammatory agents.