포도 (Vitis labrusca L.)의 직접 재분화 방법을 이용한 식물체 재분화와 형질전환

김세희,신일섭,조강희,김대현,김현란,김정희,임선형,김기옥,이향분,도경란,황해성 한국식물생명공학회 2013 식물생명공학회지 Vol.40 No.4

Efficient regeneration methods and transformation system are a priority for successful application of genetic engineering to vegetative propagated plants such as grape (Vitis labrusca L.). This research is to establish shoot regeneration system from plant explants for ‘Campbell Early’, ‘Tamnara’, ‘Heukgoosul’, ‘Heukbosek’ using two types of plant growth regulators supplemented to medium. The highest adventitious shoot regeneration rate of 5% was achieved on a medium containing of Murashige and Skoog (MS) inorganic salts and Linsmaier and Skoog (LS) vitamins, 2 mg/L of TDZ and 0.1 mg/L of IBA. Leaf tissue of ‘Campbell Early’, was co-cultivated with Agrobacterium strains, LBA4404 containing the vector pBI121 carrying with CaMV 35S promoter, gus gene as reporter gene and resistance to kanamycin as selective agent, the other Agrobacterium strains, GV3101 containing the vector pB7 WG2D carrying with mPAP1-D gene. mPAP1-D is a regulatory genes of the anthocyanin biosynthetic pathway. ‘Campbell Early’ harboring mPAP1-D gene was readily able to be selected by red color due to anthocyanin accumulation in the transformed shoot. These results might be helpful for further studies to enhance the transformation efficiency in grape.

침수조건이 포도 ‘캠벨얼리’의 광합성에 미치는 영향

강석범(Seok Beom Kang),장한익(Han Ik Jang),이인복(In Bog Lee),박진면(Jin Myeon Park),문두길(Doo-Khil Moon) 한국원예학회 2008 원예과학기술지 Vol.26 No.4

침수 시 근권 내 용존산소 공급이 포도 ‘캠벨얼리’의 광합성과 관련된 생리반응에 미치는 영향을 구명하기 위하여 2년생 유묘를 심은 포트 위 10㎝까지 물을 채워 둔 단순침수구(WL), 침수상태에서 기포발생기로 용존산소를 공급한 통기처리구(WO), 주 2회 용존산소가 풍부한 물로 교환한 침수수교환구(WE), 침수시키지 않고 -40㎪ 토양수분장력 하에서 관수점을 제어한 대조구(NC) 등 4처리 시험을 6주간 수행하였다. 29일간의 침수처리 후 새벽엽수분포텐셜은 대조구(NC)가 -0.23㎫로 가장 높았고 통기처리구(WO)가 -0.35㎫, WE -0.5㎫, WL -0.57㎫로 낮아졌으며, 외기와 엽온 간의 온도차는 대조구(NC)가 1.5℃로 처리구간에서 가장 컸고, 통기처리구(WO)는 0.9℃, 단순침수구(WL)은 0.2℃를 나타내어 통기처리에 의한 용존산소 공급에 의하여 뿌리의 수분흡수가 개선되고 엽온이 저하되었다. 침수기간이 경과함에 따라 포도 잎의 증산율(E), 기공전도도(g), 순광합성(ACO₂)은 감소하였으나 용존산소를 공급한 통기처리구(WO)는 다른 침수구들에 비해 감소가 완화되었으며, 엽온을 기준으로 한 엽과 대기간의 확산압차(VpdL)는 침수기간이 경과함에 따라 증가하였으나 통기처리구(WO)는 다른 침수구들에 비해 증가폭이 적었다. 포도 잎의 엽록소형광(Fv/Fm)에 있어서는 침수 후 8일째부터 통기처리구(WO)가 대조구를 제외한 다른 침수처리구(WE, WL)에 비해 Fv/Fm값이 높게 나타났으며 24일과 31일째에는 NC, WO와 WE 처리구간에는 유의성 없이 WL 처리구에 비해 유의하게 높았다. 이상의 결과는 침수 시 근권 내 통기처리에 의한 용존 산소 공급이 엽수분포텐셜을 증가시킴으로서 엽온 상승을 낮추고, 광합성 및 형광반응 등 수체 생리에 미치는 침수 스트레스를 완화시키는 것으로 추정된다. This experiment was conducted to investigate the effect of dissolved oxygen on the photosynthesis, and related physiological response of two-year old ‘Campbell Early’ grapevine (Vitis labrusca L.) under waterlogged condition from June 15 to July 27, 2005. For the experiment, two-year old grapevine seedlings were transplanted to 40 L pot with a sandy loam soil. Treatments consisted of non-treated control with controlled at -40 ㎪ of soil water tension (NC), waterlogging with continuous aeration to supply dissolved oxygen (WO), waterlogging with exchange of water twice a week (WE), and continuous waterlogging without aeration as well as exchange of water (WL). After 29 days of waterlogging, predawn leaf water potential of NC was the highest at -0.23 ㎫ in treatments, but other treatments were -0.35 ㎫ (WO), -0.50 ㎫ (WE), and -0.57 ㎫ (WL) respectively. Difference between air and leaf temperatures was higher in WO (0.9℃) than other waterlogging treatments except NC (1.5℃). Net CO₂ assimilation (Aco₂), stomatal conductance (gs), and transpiration rate (E) of waterlogged grapevines were decreased by continuous waterlogging but mitigated by supplying dissolved oxygen (WO). Leaf-to-air water vapor pressure deficit on leaf temperature (VpdL) was increased by the prolongation of waterlogging, but attenuated by WO treatment. From 8 days after waterlogging, Fv/Fm of WO in treatments was higher than other waterlogging treatments (WE, WO) except NC; also, there was no significant difference among the Fv/Fm of NC, WO and WE, but was significantly higher than WL at 24 and 31 days after waterlogging. Thus, it could be concluded that supplying dissolved oxygen by aeration decreases leaf temperature, increases predawn leaf water potential and Fv/Fm and resulted in alleviation of the reduction of photosynthesis of ‘Campbell Early’ grapevine under waterlogged condition.

고농도 CO₂ MA 포장이 ‘캠벨얼리’ 포도의 상온 유통 중 품질에 미치는 영향

김찬우(Chan Woo Kim),정문철(Moon Cheol Jeong),최정희(Jeong Hee Choi) 한국원예학회 2009 원예과학기술지 Vol.27 No.4

본 연구는 고농도 CO₂ MAP가 ‘캠벨얼리’ 포도(Vitis labrusca L.) 의 상온 유통 중 품질 변화에 미치는 영향을 구명하기 위해 수행되었다. 시료가 담긴 용기내부를 15, 25, 50㎪ CO₂+5㎪ O₂(N₂ balance)로 조성된 혼합가스 또는 100㎪ CO₂로 치환한 후 밀봉하였으며, 상온에서 8일간 보관하면서 중량 감소, 당도, 산도, 부패, 탈립, 갈변, 관능평가 등 품질 인자의 변화를 조사하였다. 수확 후 포도에 고농도 CO₂ 를 처리하면 과방경과 소과경의 갈변, 과실의 부패, 그리고 탈립이 감소되고 기호도가 향상되는 결과를 나타내었다. 그러나 CO₂ 농도가 50㎪ 이상 높아지면 이취가 발생하였고, 100㎪ CO₂ 처리시에는 갈변, 부패, 탈립 발생이 다른 처리구(15, 25, 50㎪ CO₂)에 비해 오히려 증가하는 결과를 나타내었다. 본 연구 결과 15와 25㎪ CO₂ MAP 처리는 이취 발생 없이 포도의 외관 향상과 품질 유지를 위한 실용적인 기술로서 적용될 수 있을 것으로 판단되었다. This study was conducted to evaluate the effect of high CO₂ modified atmosphere packaging (MAP) conditions composed of 15, 25 and 50 ㎪ CO₂+5 ㎪ O₂ (balanced with N2), and 100 ㎪ CO₂ on the quality of ‘Campbell Early’ grapes (Vitis labrusca L.). Fruit quality attributes including soluble solids content, titratable acidity, decay of berries, berry shattering, and browning of stalk and pedicel were measured during marketing simulation at ambient temperature. In CO₂-treated bunches, the browning of stalk and pedicel, the decay of berries, and shattering were inhibited, and the organoleptic score was higher than in non-treated bunches. MAP with 50 and 100 ㎪ CO₂, however, resulted in off-flavour. When 100 ㎪ CO₂ was used to grapes, browning, decay, and shattering of bunches actually increased compared with the 15, 25, and 50 ㎪ CO₂-treated ones. Our results, therefore, showed that high CO₂ MAP with 15 and 25 ㎪ could be applied to grapes as a practical technique for improving appearance of the bunches and inhibiting the browning, decay, and shattering of the berries without off-flavour.

전자선 조사한 캠벨얼리 포도(Vitis Labrusca L.)의 휘발성 유기성분 변화

심성례 ( Sung Lye Shim ),노기미 ( Ki Mi No ),김경수 ( Kyong Su Kim ),송기동 ( Gi Dong Song ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2010 한국식품저장유통학회지 Vol.17 No.1

포도근 약침액이 LPO, RNS 및 Cytochrome P450에 미치는 효과

임성철 ( Seong Cheorl Lim ),김혁 ( Hyuck Kim ),이효승 ( Hyo Seung Yi ),박원환 ( Won Hwan Park ),문진영 ( Jin Young Moon ) 대한경락경혈학회 2007 Korean Journal of Acupuncture Vol.24 No.1

Objectives: In this study, we determinated protective effects of Vitis labrusca Root herbal-acupuncture solution (VLHA) against atherosclerosis and cardiovascular disease induced by oxidative damage and cytochrome P450 (CYP) induction. Methods: Antioxidant capacity of VLHA were evaluated by the β-carotene/linoleic acid emulsion system and FeCl2-ascorbic acid stimulated lipid peroxidation in rat liver homogenate, respectively. Scavenging effects of VLHA on ONOO- and NO were measured by DHR 123, DAF-2 assay. Furthermore, we measured inhibitory activity of VLHA on CYP 2E1 in rat liver microsome using by Aniline assay. Results: VLHA exhibited potent inhibition rate(74%) on FeCl2-ascorbic acid induced lipid peroxidation, and revealed a strong scavenging effects on ONOO- and NO. In addition, VLHA showed inhibitory effect on CYP 2E1. Conclusions: The present study concludes that our results suggest that VLHA could be used as a good source in the prevention of atherosclerosis and alcoholic liver injury.

Anti-Atherogenic Effects of a Phenol-Rich Fraction from Brazilian Red Wine (Vitis labrusca L.) in Hypercholesterolemic Low-Density Lipoprotein Receptor Knockout Mice

Mariana Appel Hort,Elke Zuleika Schuldt,ˆ ngela Cristina Bet,Silvia DalBo,Jarbas Mota Siqueira,,Carla Ianssen,Fa´tima Abatepaulo,Heraldo Possolo de Souza,Beatriz Veleirinho,Marcelo Maraschin,Rosa Mari 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.10

Moderate wine intake (i.e., 1–2 glasses of wine a day) is associated with a reduced risk of morbidity and mortality from cardiovascular disease. The aim of this study was to evaluate the anti-atherosclerotic effects of a nonalcoholic ethyl acetate fraction (EAF) from a South Brazilian red wine obtained from Vitis labrusca grapes. Experiments were carried out on low-density lipoprotein (LDL) receptor knockout (LDLr−/−) mice, which were subjected to a hypercholesterolemic diet and treated with doses of EAF (3, 10, and 30 mg/kg) for 12 weeks. At the end of the treatment, the level of plasma lipids, the vascular reactivity, and the atherosclerotic lesions were evaluated. Our results demonstrated that the treatment with EAF at 3 mg/kg significantly decreased total cholesterol, triglycerides, and LDL plus very low-density lipoprotein levels compared with control hypercholesterolemic mice. The treatment of mice with EAF at 3 mg/kg also preserved the vasodilatation induced by acetylcholine on isolated thoracic aorta from hypercholesterolemic LDLr−/− mice. This result is in agreement with the degree of lipid deposit on arteries. Taken together, the results show for the first time that the lowest concentration of an EAF obtained from a red wine produced in southern Brazil significantly reduced the progression of atherosclerosis in mice.

포도 전정가지의 화장품 소재로서의 응용

양재황(Jae Hwang Yang),백성환(Baek sung hwan),박동우(Dong Woo Park),전동하(Dong Ha Jun),김극준(Kim Geuk-Jun),장민정(Min Jung Jang) 한국생명과학회 2014 생명과학회지 Vol.24 No.6

포도는 전 세계적으로 가장 많이 재배되는 과수 중의 하나로 포도 및 포도 가공제품에 대한 성분분석, 항산화, 항암 등 다양한 생물학적 효능 연구가 이루어지고 있으나 겨울처 전정되어 버려지는 전정가지에 대한 연구는 미미한 실정이다. 본 연구에서는 포도전정가지 추출물(GPSE)의 총 폴리페놀함량, 항산화활성, 미백 및 주름개선효과를 검증하였다. 먼저 HPLC분석에는 GPSE는 614.05 ug/g의 trans-resveratrol을 함유하고 있는 것으로 관찰되었다. DPPH 라디칼소거능, ABTS 라디칼소거능 등의 항산화 실험을 통해서 대조군으로 사용된 BHA와 L-ascrobic acid에 비교하여 GPSE은 높은 항산화 활성을 나타내었다. 미백활성 측정을 위한 tyrosinase 저해활성 실험에서도 GPSE는 15.35%, arbutin은 1.48%로 GPSE의 미백효과가 높게 나타났으며, 주름개선 실험에서는 대조군과 비슷한 효과를 가지고 있는 것을 확인 하였다. 이와 같은 결과로 미루어 보아 GPSE는 trans-resveratrol 함량이 높은 천연 항산화제로 기능성 화장품 및 식품으로의 가능성을 확인하였다. Grape pruning stems constitute a scarcely investigated class of byproducts with limited reports on their bioactive polyphenol content and/or industrial applications. Herein we present the outcome of our investigation on grape pruning stems extracts, concerning the assessment of their total polyphenolic content and the detailed evaluation of their antioxidant properties. Results obtained indicate that grape pruning stems are particularly rich in flavonoids and trans-resveratrol. The antioxidant activities was analyzed and expressed as electron donating ability, ABTS cation radical decolorization, hydrogen peroxide scavenging activity and superoxide anion radical scavenging activity, the antioxidant activity of Vitis labrusca L. pruning stems extracted (GPSE) was higher than that of BHA and L-ascrobic acid. The whitening and anti-wrinkle activities display an capability. Results herein grape pruning stems used as a valuable resource for the extraction of resveratrol, which would be added to functional cosmetics and food materials.

Influence of petiole mineral content on fruit quality of ‘Campbell Early’ grape

Seong Eun Lee(이성은),Jin Myeon Park(박진면),Bong Nam Chung(정봉남),Pyoung Ho Yi(이평호) 한국토양비료학회 2016 한국토양비료학회 학술발표회 초록집 Vol.2016 No.10