Fibrin Scalant 를 사용한 골자가이식에서 혈관신생에 관한 실험적 연구

권성택,김병건,김진환 大韓成形外科學會 1993 Archives of Plastic Surgery Vol.20 No.3

The effect of fibrin to wound healing has been studied and well documented : homostatic and adhesive effect and stimulatory effect on proliferation of fibroblast, formation of granulation tissue and neovascularization has been well known. In the process of bone healing, action of fibrin has been reported to stimulate the formation of granulation tissue, new vessels and new bone, but there have been many reports contrary to the effects of fibrin. Authors used New Zealand white rabbit as an experimental animal. Bone grafts were harvested from right and left parietal bone and onlay grafts were performed to both sides of frontal bone. Fibrin sealant was applied to experimental group and was not applied on the control group. Both groups were sacrificed after 1, 2, 3, 4, and 8 weeks respectively and results were examined and compared after H&E stain and Masson's trichrome stain by light microscope. The group using fibrin sealant showed new bone formation on 1st week and new vessel formation on 2nd week, and vessels were numerous and had thick walls. And there were dense bony connections between donor bone and recipient bone. In calvarial bone graft which is most commonly used in plastic and reconstructive surgery, especially in infected and extensively injured wound which might compromise blood supply, we believe that bone graft using fibrin sealant can stimulate vascularization and new bone formation and therefore enhance the survival of bone graft.

스트렙토조토신 유도 당뇨백서의 골조직 재생에 흡수성 차폐막이 미치는 영향

양병근,이학철,이지영,손강배,설양조,이상철,계승범,정종평,한수부,Yang, Byung-Kun,Lee, Hak-Churl,Lee, Ji-Young,Son, Kang-Bae,Seol, Yang-Jo,Lee, Sang-Cheol,Kye, Seung-Beom,Chung, Chong-Pyoung,Han, Soo-Boo 대한치주과학회 2000 Journal of Periodontal & Implant Science Vol.30 No.2

The purpose of this study is to evaluate the effects of bioresorbable membranes in guided bone regeneration of streptozotocin induced diabetic rats. 50 Sprague-Dawley rats were randomly categorized into 4 groups: Group 1 & 2 had 10 normal rats each and group 3 & 4 included 15 streptozotocin induced diabetic rats each. Defect measuring 7mm in diameter was formed on every rat calvarium. No membrane was used in groups 1 & 3 and membranes were used in groups 2 & 4. The rates were sacrificed at 2 and 4 weeks after defect formation. Routine histological specimens were prepared. Masson-trichrome and HE stain were done before light microscopy. Guided regenerative potential was evaluated by measuring the amount of new bone formation in the calvarial defect by histomorphometry. Following results were obtained. 1. New bone formation in the diabetic groups was significantly less that than in the normal groups regardless of membrane use(p<0.05). 2. In the comparison of new bone formation in the normal groups, membrane group showed significantly more bone formation(p<0.1). 3. When the amount of new bone formation was compared in the diabetic groups, more bone was formed in the membrane groups but the difference was not statistically significant.4. In the normal groups the amount of new bone formation was significantly greater at 4 weeks compared to that at2 weeks(p<0.05) but amount of bone regeneration at 4 weeks was not significantly greater than that at 2 weeks in both diabetic groups.

발치와의 신생골 형성에 미치는 탈회동결건조골과 흡수성 수산화인회석의 영향

정숭룡,김선헌,류선열 大韓顎顔面成形再建外科學會 1995 Maxillofacial Plastic Reconstructive Surgery Vol.17 No.3

The present study was aimed to evaluate the effect of demineralized freeze-dried bone (DFDB) and resorbable hydroxyapatite (RHA) on bone formation in the extracted socket. The lower left and right 2nd and 3rd premolar were extracted in adult dogs. The one group was grafted with DFDB into the extracted socket, and the other group grafted with RHA. The extracted socket was sutured without any graft materials as control. The animals were killed on the 1st, 2nd, 4th, and 8th week after the graft for macroscopic and microscopic examination. Results obtained were as follows : 1. Macroscopically, nor infection of the graft site and dislodgement of the grafted material were noted in any animals used. 2. Young trabeculae of osteoid were formed in the socket wall in control group at 2 weeks after the graft. Osteoid tissue was formed in DFDB group at 1 week after graft, and a fine osteoid tissue was grown through the RHA particles in RHA group at 2 weeks graft. 3. The grafted groups showed more rapid bone formation tha n the control. Between the grafted groups, DFDB group showed more rapid formation than RHA group, DFDB group showed osteoinductive bone formation and RHA group showed osteoconductive bone formation. These results suggest that DFDB and RHA are useful to preserve the alveolar bone and to improve new bone formation by immediate grafting into the extraction sockets.

가토 두개골 결손부에서 베타-삼칼슘 인산염 이식 시 혈소판 풍부 섬유소와 혈소판 풍부 혈장의 골형성능에 관한 연구

박정균,주현중,이의석,장현석,임재석,권종진,Park, Jeong-Kyun,Joo, Hyun-Jung,Lee, Ei-Seok,Jang, Hyon-Seok,Lim, Jae-Seok,Kwon, Jong-Jin 대한악안면성형재건외과학회 2011 Maxillofacial Plastic Reconstructive Surgery Vol.33 No.1

Purpose: Addition of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) to grafting material has become widely accepted additively for bone regeneration because it can raise high expectations on it's clinical potential. The aim of this study was to evaluate the efficacy of PRP and PRF on early bone regeneration of rabbits when used in combination with beta tricalcium phosphate. Methods: In eight rabbits, the calvarium was exposed and the two marrows were penetrated. After then these artificial bone defects were augmented with ${\beta}$-TCP or ${\beta}$-TCP with PRP or ${\beta}$-TCP with PRF and covered. The animals were sacrificed after four and eight weeks. Histologic findings were observed under the light-microscope and histomorphometric analysis was performed by measuring calcified area of new bone formation within the CSD. Results: They demonstrated that new bone formation tended to be produced along the outline of graft materials. More amounts of newly bone was regenerated in ${\beta}$-TCP only and in combination of${\beta}$-TCP with PRF and it was statistically significant. In contrast, there was no significant difference between nothing apply and ${\beta}$-TCP with PRP groups in the relative amounts of newly mineralized bone. Conclusion: Within the limitation of this study, it can be concluded that PRF in combination with ${\beta}$-TCP showed a positive effect on bone regeneration and statistically it was significant.

성장호르몬의 골모세포 및 신생골형성에 대한 효과

송해룡,강영진,장기철,연성찬,구자민,김현희 대한골절학회 2003 대한골절학회지 Vol.16 No.3

목적: 성장호르몬이 신생골형성 및 골모세포에 미치는 영향을 분석하고자 한다. 대상 및 방법: 실험쥐 37마리에서 경골의 결손을 만든 다음 외고정기구를 장착시키고 성장호르몬을 8주간 근육주사한 군 25마리, 증류수액을 주사한 대조군 12마리를 이용하여 신생골형성을 곤찰하였고 신생골부위의 골밀도를 측정하였다. 쥐의 골육종에서 추출된 골아세포에 생성유뮤등을 관찰하였다. 결과: 성장호르몬을 투여한 군에서 골결손부위의 신생골의 골밀도가 높은 것으로 나타났으며 통계학적으로 유의한 차이가 있었다. 외고정기구를 장착한 경골의 근위부보다는 원위부에서 성장호르몬을 투여한 실험군에서 골밀도가 대조군보다 높았으며 통계학적으로 유의한 차이가 있었다. 성장호르몬을 투여한 실험군에서 대조군과 비교할시 배양 5일에 골모세포수의 감소가 지연되었으며 alkaline phosphatase의 활성 및 osteocalcin mRNA의 발현이 증가되었다. 결론: 성장호르몬의 투여는 골모세포의 증식 및 신생골형성 촉진 효소의 발생을 유도함으로써 골절로 인한 골결손부위의 골유합기간을 단축시킬 수 있으며 골절 후 체중부하의 지연으로 발생하는 골다공증을 예방할 수 있다고 사료된다. Purpose: To evaluate effect of growth hormone on osteoblast and new bone formation. Materials and Methods: Bone defect of the tibia with preserved periosteum was made and fixed with external fixator. Intramuscular injection of growth hormone for 8 weeks in experimental group and saline in control group was performed. New bone formation at the bone defect in radiograph and bone mineral density (BMD) by quantitative computed tomography were evaluated at 8 weeks after surgery. Rat osteosarcom cells were cultured in both group to evaluate cell viability of osteoblast, alkaline phosphatase activity, and mRNA expression of osteoclcin by RT-PCR. Result: Experimental group showed more cellus formation and higher BMD at the bone detect site and the distal tibia compared to control group and there was significant difference. Proliferation of osteoblast, alkaline phosphatase activity, and mRNA of osteoclcin at 5 days after culture were significantly higher in experimental group than those in control group. Conclusion: Growth hormone has positive effect on osteoblast and cellus formation in vivo and vitro studies.

가토 요골 간부 골 결손시에 혈관 부착 골막 보존 유무에 따른 신생골 형성에 대한 실험적 연구

정덕환,염재광,태석기,고광원,Chung, Duke-Whan,Yum, Jae-Kwang,Tae, Suk-Kee,Ko, Kwang-Won 대한미세수술학회 1999 Archives of reconstructive microsurgery Vol.8 No.2

The osteogenic capacity of the vascularized periosteum autograft has been extensively demonstrated by experimental works. The objective of this study was to characterize the behavior of experimental model of vascularized periosteal flap(VPF) by observing sequential stages of osteogenesis after simulated VPF in rabbits. In experimental group, segmental resection of bone including the periosteum was performed in 22 radii of 22 New Zealand white rabbits preserving the periosteal circulation of median artery to the periosteum. In order to simulate the transplantation of VPF, the vascular pedicle consisting of median artery and veins was dissected from adjacent soft tissue and the periosteum was longitudinally incised to remove the bone followed by repair of the periosteum. From the first to sixteenth week after the simulated VPF, the changes in VPFs were observed by radiological, light microscopical, scanning electron microscopical methods and the activity of osteocalcin was measured by immunohistochemical method. In control group, the bone tissue and periosteum were completely removed from the mid-shaft of radius and the findings were observed by radiological and light microscopical methods. From the results of this study, it is demonstrated that the experimental model of VPF is vigorously and uniformly osteogenic. Therefore it is thought that VPF can be used as a measure to treat bone defect of shaft of long bone.

Histomorphometric evaluation for osseointegration after particulated-dentin coated implant placement

( Jae Seek You ),( Su Gwan Kim ),( Ji Su Oh ),( Sung Chul Lim ),( Jae Sung Kim ) 조선대학교 치의학연구원 2015 Oral Biology Research (Oral Biol Res) Vol.39 No.1

Purpose: The purpose of this study was to evaluate osseointegration after placement of a machined surface implant and particulated-dentin-coated implant in a canine model. Materials and Methods: Four dogs were randomly assigned to two groups, and each group was further divided into two subgroups 4 and 8 weeks after implantation. The implant was placed under different conditions. Group 1 included the machined surface implant and group 2 included the particulated-dentin-coated implant. Histologic sections and histomorphometric analysis were obtained 4 and 8 weeks after surgery. Results: Group 2 showed a higher bone-implant contact rate and bone formation rate than group 1 in the 8 week group. In group 2, the 8 week group revealed significant elevation of both bone-implant contact rate and new bone formation rate versus the 4 week group. Conclusion: According to these results, particulate-dentin-coated implants can provide satisfactory stability and increase the quantity and maturity of new bones in the later stages of implant placement.

성견에 식립한 인산칼슘 피복 임플란트가 골조직 유착에 미치는 생물학적인 영향

심언철,임성빈,정진형,김종여,Shim, Eon-Cheol,Lim, Sung-Bin,Chung, Chin-Hyung,Kim, Jong-Yeo 대한치주과학회 2003 Journal of Periodontal & Implant Science Vol.33 No.4

The influence of calcium phosphate (Ca-P) coating on the bone response of titanium implants was investigated two types of titanium implants, i.e. as -machined ,as -machined with Ca-P coating, were prepared. The Ca-P coating produced by OCT Inc technique. These implants were inserted into the left and right femur of beagle dog, After implantation periods of 3 days, 1weeks, weeks, 4weeks, 8weeks, 12weeks. 24weeks, the bone-implant interface was evaluated histologically, histomorphometrically , and removal torque. Histological evaluation revealed no new bone formation around different implant materials after 2weeks of implantation. After 4 weeks, Ca-P coated implants showed a higher amount of bone contact than either of the non coated implants. After 12weeks, bone healing was almost completed. And implant were removed by reverse torque rotation with torque-measuring device. Mean torque values for 4weeks control were 2.375Kgf.cm and experimental were 2.725Kgf.cm. And mean torque values for 8weeks control were 1.25Kgf.cm and experimental were 1.0Kgf.cm On the basis of these findings, we concluded that deposition of a Ca-P coating on an implant has a beneficial effect on the bone response to this implant during the healing phase. Besides implant surface conditions the bone response is also determined by local implant site condition.

하악골 신연술 후 생성된 신생골의 조직학적 및 면역화학적 소견

윤경인,박재억 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.3

Distraction osteogenesis(DO) can be performed in the bony defect associated with trauma, anomaly, and various kinds of disease. The gap generated by DO is filled with growing callus : during the period of distraction, the osteogenesis is continued. However, there have been few reports about expression pattern of growth factors in newly formed bone during the consolidation periods. We performed DO in the mandibular defect case and studied the expressed pattern of growth factors. Its pattern was compared to that of the same patient. BMP-2 and -4 were strongly expressed in the DO site. Particularly, BMP-4 was not expressed in the normal mature bone, but expressed in new bone in DO. However, there was no difference in the FGF-7 expression between the sites. Therefore, strong expression of BMP-4 are related to new bone formation in DO and they may not be related to the normal homeostasis in human bone. Though FGF-7 is related to the growth of keratinocyte, it may have minimal role in the DO and normal mature bone.

성견 치주질환 발치와에 이식된 구연산 처리 치근의 치유에 대한 연구

지준순,김종관,Chi, Jun-Soon,Kim, Chong-Kwan 대한치주과학회 1993 Journal of Periodontal & Implant Science Vol.23 No.2

The author transplanted periodontally-diseased teeth which had been treated with citric acid into a clinically healthy extraction sockets and periodontally-affected extraction sockets, and compared with the healing processes within these tissues. Recipient sites were prepared by surgically removing a part of alveolar bone of premolars of adults dogs, placing elastic orthodontic ligatures for 8weeks, thereby inducing periodontal disease. The diseased roots were extracted and transplanted into healthy extraction sockets, and these were designated as control group 1. Diseased roots transplanted into diseased sockets were designated as control group 2. Diseased roots which had been root planed, treated with citric acid and transplanted into healthy sockets were designated as experimental group 1, while identically treated roots which had been transplanted into diseased sockets were designated as experimental group 2. Observations were made at weeks 2, 8 and 12, with following results. 1. At week 2, experimental group 2 showed some inflammatory cell infiltration in the connective tissue above the extraction sockets, while control groups showed less inflammatory or foreign body reactions throughout the experiment. 2. In both control groups, root surface resorption was observed throughout the experiment, while experimental groups showed a little resorption. 3. Control group 1 & 2 showed ankylosis by newly-formed bone ground the resorbed root surfaces, while experimental group 1 & 2 displayed collagen fibers which are not functionally-arranged, with random, loose arrangement or parallel orientation to root surfaces, and newly-formed bone outside of them. 4. In both control groups & experimental groups which had been transplanted into a clinically healthy extraction sockets & periodontally affected extraction sockets groups, histological differences were not significant. 5. Root resorption or ankylosis in control group 1 & 2 had increased quantitatively as experiment progressed. 6. New bone formation developed from the base and lateral wall of extraction sockets. In both control groups & experimental groups, root surfaces lying next to the upper portion of extraction sockets showed little alveolar bone formation and surrounded by connective tissue fiber at weeks 2 & 8, while at weeks 12, they did show alveolar bone formation. 7. At week 12, experimental group 2 showed numerous cells which appeared to be periodontal ligament cells, with functionally arranged connective tissue fibers between the roots and alveolar bone.