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Diphenylhydantoin이 배양중인 치은 섬유아세포에 미치는 영향
임성빈,이재현 단국대학교 치의학연구소 1991 논문집 Vol.3 No.-
The purpose of this study was to evaluate the effect of diphenylhydantion on humna gingival fibroblasts in culture. Cell proliferation assay was performed to determine the effect of PHT on the DNA synthesis rate of human gingival fibroblasts by means of [^3H]-thymidine incorporation method. Protein synthesis assay was designed to detect the collagen production by [^3H]-proline incorporation method. The results were as follow : 1. In case of DNA synthesis assay, the contents of DNA were significantly increased more than the control group(1 ㎍/ml : p<0.05, 5 ㎍/ml, 10 ㎍/ml : p<0.01). 2. In case of collagenase-digestible protein synthesis assay, the contents of CDP were significantly increased on PHT 5 ㎍/ml group compare to the control group(p<0.05), and PHT 1 ㎍/ml, 5 ㎍/ml group were increased but there were statistically not significant. 3. In case of noncollageneous protein synthesis assay, the contents of NCP were significantly increased on PHT 1 ㎍/ml group(p<0.05), and PHT 5 ㎍/ml, 10 ㎍/ml group were increased but there were statistically not significant. 4. The ability of collageneous-digestible protein synthesis was great increased than the cell proliferation. 5. The effects of PHT increasing the ability of collagen synthesis, were cell proliferation and increased of protein synthesis.
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