Hypolipidemic and Antioxidant Effects of Dandelion ( <i>Taraxacum officinale</i> ) Root and Leaf on Cholesterol-Fed Rabbits

Choi, Ung-Kyu,Lee, Ok-Hwan,Yim, Joo Hyuk,Cho, Chang-Won,Rhee, Young Kyung,Lim, Seong-Il,Kim, Young-Chan Molecular Diversity Preservation International (MD 2010 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.11 No.1

<P>Dandelion (<I>Taraxacum officinale</I>), an oriental herbal medicine, has been shown to favorably affect choleretic, antirheumatic and diuretin properties. Recent reports have indicated that excessive oxidative stress contributes to the development of atherosclerosis-linked metabolic syndrome. The objective of this current study was to investigate the possible hypolipidemic and antioxidative effects of dandelion root and leaf in rabbits fed with a high-cholesterol diet. A group of twenty eight male rabbits was divided into four subgroups; a normal diet group, a high-cholesterol diet group, a high-cholesterol diet with 1% (w/w) dandelion leaf group, and a high-cholesterol diet with 1% (w/w) dandelion root group. After the treatment period, the plasma antioxidant enzymes and lipid profiles were determined. Our results show that treatment with dandelion root and leaf positively changed plasma antioxidant enzyme activities and lipid profiles in cholesterol-fed rabbits, and thus may have potential hypolipidemic and antioxidant effects. Dandelion root and leaf could protect against oxidative stress linked atherosclerosis and decrease the atherogenic index.</P>

Anti-Obesity and Hypolipidemic Effects of Lycium chinense Leaf Powder in Obese Rats

Myung-Hwa Kang,Won-Jong Park,Mi-Kyeong Choi 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.4

This study aimed to elucidate the obesity control, hypolipidemic, and antioxidant effects of Lycium chinense leaf powder intake by obese rats. Obesity was induced in rats through 13 weeks of high-fat diet. The obese rats were then divided into four different groups, which were fed for 8 weeks with general diet (G), high-fat diet (F), 5% L. chinense leaf powder with a high-fat diet (FLP5), or 10% L. chinense leaf powder with a high-fat diet (FLP10). The body weight gain of the FLP5 group was significantly lower than that of the F group. Also, the obesity index of the FLP5 and FLP10 group was significantly lower than that of the F group. Serum triglyceride and low-density lipoprotein (LDL)-cholesterol levels in the FLP5 group were significantly lower than those of the F group. The intake of L. chinense leaf powder did not seem to significantly affect the levels of serum homocysteine, leptin, and ghrelin compared to the control group without L. chinense leaf powder intake. The glutathione content in the liver was significantly higher in the FLP5 group than in the G group, but the glutathione S-transferase activity was significantly lower than in the F group. The thiobarbituric acid-reactive substances levels in the liver and kidney were relatively lower in the FLP5 and FLP10 groups than in the G group. In summary, intake of L. chinense leaf powder in obese rats coincided with a lowering of body weight and levels of serum triglyceride and LDL-cholesterol. It also displayed antioxidant effects.

Blueberry, blackberry, and blackcurrant differentially affect plasma lipids and pro-inflammatory markers in diet-induced obesity mice

Ji-Young Lee,김보경,Sang Gil Lee,Young-Ki Park,Chai Siah Ku,Tho X. Pham,Casey J. Wegner,Yue Yang,Sung I. Koo,Ock K. Chun 한국영양학회 2016 Nutrition Research and Practice Vol.10 No.5

BACKGROUND/OBJECTIVES: Evidence indicates that berry anthocyanins are anti-atherogenic, antioxidant, and anti-inflammatory. However, berries differ vastly in their anthocyanin composition and thus potentially in their biological and metabolic effects. The present study compared hypolipidemic, antioxidant, and anti-inflammatory properties of blueberry (BB), blackberry (BK), and blackcurrant (BC) in a diet-induced obesity (DIO) mouse model. MATERIALS/METHODS: Male C57BL/6J mice were fed a high fat (HF; 35% fat, w/w) control diet or a HF diet supplemented with freeze-dried 5% BB, 6.3% BK or 5.7% BC for 12 weeks (10 mice/group) to achieve the same total anthocyanin content in each diet. Plasma lipids, antioxidant status and pro-inflammatory cytokines were measured. The expression of genes involved in antioxidant defense, inflammation, and lipid metabolism was determined in the liver, epididymal adipose tissue, proximal intestine, and skeletal muscle. Histological analysis was performed to identify crown-like structure (CLS) in epididymal fat pads to determine macrophage infiltration. RESULTS: No differences were noted between the control and any berry-fed groups in plasma levels of liver enzymes, insulin, glucose, ferric reducing antioxidant power, superoxide dismutase, and tumor necrosis factor α. However, BK significantly lowered plasma triglyceride compared with the HF control and other berries, whereas BC significantly reduced F4/80 mRNA and the number of CLS in the epididymal fat pad, indicative of less macrophage infiltration. CONCLUSIONS: The present study provides evidence that BB, BK and BC with varying anthocyanin composition differentially affect plasma lipids and adipose macrophage infiltration in DIO mice, but with no differences in their antioxidant capacity and anti-inflammatory potential.

Blueberry, blackberry, and blackcurrant differentially affect plasma lipids and pro-inflammatory markers in diet-induced obesity mice

Kim, Bohkyung,Lee, Sang Gil,Park, Young-Ki,Ku, Chai Siah,Pham, Tho X.,Wegner, Casey J.,Yang, Yue,Koo, Sung I.,Chun, Ock K.,Lee, Ji-Young The Korean Nutrition Society 2016 Nutrition Research and Practice Vol.10 No.5

BACKGROUND/OBJECTIVES: Evidence indicates that berry anthocyanins are anti-atherogenic, antioxidant, and anti-inflammatory. However, berries differ vastly in their anthocyanin composition and thus potentially in their biological and metabolic effects. The present study compared hypolipidemic, antioxidant, and anti-inflammatory properties of blueberry (BB), blackberry (BK), and blackcurrant (BC) in a diet-induced obesity (DIO) mouse model. MATERIALS/METHODS: Male C57BL/6J mice were fed a high fat (HF; 35% fat, w/w) control diet or a HF diet supplemented with freeze-dried 5% BB, 6.3% BK or 5.7% BC for 12 weeks (10 mice/group) to achieve the same total anthocyanin content in each diet. Plasma lipids, antioxidant status and pro-inflammatory cytokines were measured. The expression of genes involved in antioxidant defense, inflammation, and lipid metabolism was determined in the liver, epididymal adipose tissue, proximal intestine, and skeletal muscle. Histological analysis was performed to identify crown-like structure (CLS) in epididymal fat pads to determine macrophage infiltration. RESULTS: No differences were noted between the control and any berry-fed groups in plasma levels of liver enzymes, insulin, glucose, ferric reducing antioxidant power, superoxide dismutase, and tumor necrosis factor ${\alpha}$. However, BK significantly lowered plasma triglyceride compared with the HF control and other berries, whereas BC significantly reduced F4/80 mRNA and the number of CLS in the epididymal fat pad, indicative of less macrophage infiltration. CONCLUSIONS: The present study provides evidence that BB, BK and BC with varying anthocyanin composition differentially affect plasma lipids and adipose macrophage infiltration in DIO mice, but with no differences in their antioxidant capacity and anti-inflammatory potential.

자소엽 주정 추출물이 고지방식이로 유도된 대사증후군 및 비알코올성 지방간 개선에 미치는 영향

최지영,김지원,권은영 한국식품영양과학회 2020 한국식품영양과학회지 Vol.49 No.10

Perilla frutescens Britton var. acuta Kudo (PF) is a traditional herb that belongs to the lamiaceae family. PF has been reported to have numerous biological activities, including antioxidant, anti-allergic, anti-inflammatory, anticancer, and neuroprotection effects. This study examined the effects of PF ethanol extract (PFE) on obesity and its complications, particularly focusing on non-alcoholic fatty liver disease (NAFLD) in C57BL/6J mice fed high-fat diets (HFD). Male mice were fed a normal diet (ND), a HFD, or HFD+PFE (1% w/w, PFE) for 12 weeks. The PFE treatment ameliorated dyslipidemia and hyperglycemia HFD-induced obese mice coupled with the enhanced energy expenditure. The liver weight and hepatic lipotoxicity markers (GOT and GPT) were reduced significantly in the PFE group than in the HFD group. Moreover, the hepatic lipid profiles and morphological analysis showed less hepatic lipid accumulation in the PFE group than the HFD-fed mice. In particular, the expression of hepatic lipogenic genes (Acc1 and Cidea) was down-regulated, whereas the expression of fatty acid oxidation-related genes (Pgc1α and Pparα) was increased. These results suggest that PFE can prevent or attenuate the development of HFD-induced obesity and NAFLD. 비알코올성 지방간은 비만 및 인슐린 저항성을 바탕으로 하는 대사증후군의 원인 질병으로 최근 그 발병률이 증가하고 있다. 본 연구진은 고지방식이에 의해 유도되는 비만 마우스 동물모델에 자소엽 주정 추출물을 식이에 보충하여 급여한 후 비만으로 인해 나타나는 표현형 분석과 유전자 발현 분석을 통해 대사증후군 및 비알코올성 지방간 억제 효과를 검토하였다. 자소엽 주정 추출물 보충으로 인한 체중 및 식이 섭취량은 차이가 없었으나 에너지 소비량 증가와 부고환 백색지방조직의 지방세포 크기 감소에 기여하였다. 또한, 자소엽 주정 추출물 섭취군의 경우 고지방식이군에 비해 혈장 총 콜레스테롤, 중성지방 및 아디포카인 농도가 유의적으로 감소하여 혈액의 지질 수준 및 아디포카인 불균형이 개선되었고 공복혈당 수준도 유의적으로 감소하였다. 고지방식이 섭취에 의해 증가한 간조직 중량 및 지질 축적은 자소엽 주정 추출물 보충으로 인해 현저하게 감소하였다. 마찬가지로, 간 독성 평가 지표인 혈장 GOT와 GPT 수준도 자소엽 주정 추출물 섭취군에서 유의적으로 감소하였다. 간 조직 내 지질 합성에 관련된 유전자인 Acc1 및 Cidea의 발현 수준은 자소엽 주정 추출물 보충에 의해 고지방식이군 보다 유의적으로 감소하였고, 지방산 산화 관련 유전자인 Pgc1α 및 Pparα의 발현은 자소엽 주정 추출물 섭취군에서 유의하게 증가하였다. 본 실험 결과를 종합하여 볼 때 자소엽 주정 추출물 첨가는 에너지 소비량 증가 및 간조직의 지질대사 조절 유전자발현 변화를 통한 혈중 지질 수준과 간조직 지질 수준을 개선 시켰으며 이러한 결과는 고지방식이로 유도된 비만 및 비알코올성 지방간 개선에 긍정적으로 기여할 수 있을 것으로 판단된다.

고지방사료를 섭취한 C57BL/6N 마우스에서 한천식이섬유의 혈청 지질과 인슐린 농도 저해효과

박진주(Jin Ju Park),김지은(Ji Eun Kim),윤우빈(Woo Bin Yun),이미림(Mi Lim Lee),최준영(Jun Young Choi),송보람(Bo Ram Song),김동섭(Dong Seob Kim),이충열(Chung Yeoul Lee),이희섭(Hee Seob Lee),임용(Yong Lim),정민욱(Min Wook Jung),황대연(Dae 한국생명과학회 2017 생명과학회지 Vol.27 No.8

본 연구에서는 한천식이섬유(Diet fiber of agar)가 지방과 포도당대사에 미치는 효과를 연구하고자, 고지방사료(High fat diet, HFD)를 급여한 C57BL/6 마우스에 한천함유음료(beverage contained agar, BCA)를 9주 동안 투여하고 지방축적, 지질농도, 인슐린농도 등의 변화를 분석하였다. 식이섭취효율은 HFD+BCA 처리그룹에서 유의적으로 감소하였지만, 체중과 식이섭취량은 HFD+BCA 처리그룹에서 일정하게 유지되었다. 또한, 지방조직과 간조직에서 지방의 축적은 HFD+Vehicle처리그룹에 비하여 HFD+BCA 처리그룹에서 낮게 관찰되었다. 더불어, BCA처리후에 유의적인 변화는 total cholesterol (TC)과 LDL에서 관찰되었지만, HDL의 농도는 BCA처리에 의해 변화가 없었다. 인슐린의 농도는 HFD+BCAMi 처리그룹과 비교하여 HFD+BCALo과 HFD+BCAMi 처리그룹에서 유의적으로 감소하였다. 따라서 이러한 결과는 한천식이섬유의 장기간 투여는 고지방사료를 급여한 동물에서 지방축적, 혈청내 지방프로파일, 인슐린농도의 개선을 유도할 수 있음을 제시하고 있다. 또한, 이러한 결과는 한천식이섬유를 함유하는 음료는 혈청내 지질과 인슐린 작용을 유도할 수 있음을 제시하고 있다. To investigate the beneficial effects of fiber derived from agar on the lipid and glucose metabolism of obese model mice, changes in the fat accumulation, lipid content, and insulin concentration were measured in C57BL/6N mice fed a high-fat diet (HFD) and treated with a beverage containing agar (BCA) for 9 weeks. The feed efficiency ratio was significantly decreased in the HFD+BCA treatment group, although the body weight and food intake were maintained a constant level. Also, the fat accumulation in the fatty tissue and liver were lower in the HFD+BCA treatment group than in the HFD+Vehicle treatment group. Furthermore, a significant decrease was detected in the levels of total cholesterol (TC) and LDL after the BCA treatment, while a constant HDL level was maintained in the same group. Moreover, the serum insulin concentration was significantly decreased in the HFD+ BCALo (Low concentration) and HFD+BCAMi (Middle concentration) groups compared to the HFD+ Vehicle group. Therefore, the results suggest that the long-term administration of dietary fiber from agar can improve fat accumulation in the fatty tissue and liver, the serum lipid profile, and the insulin concentration in the blood. Also, the results provide evidence that the inclusion of agar fiber in beverages results in significant hypolipidemic and hypoinsulinemic effects.

Hypolipidemic Effect of Exo-Polymer Produced in Submerged Mycelial Culture of Five Different Mushrooms

( Byung Keun Yang ) 한국미생물생명공학회 2002 Journal of microbiology and biotechnology Vol.12 No.6

Antidiabetic and Hypolipidemic Effects of Collybia confluens Mycelia Produced by Submerged Culture in Streptozotocin-Diabetic Rats

Yang, Byung-Keun,Jeong, Sang-Chul,Lee, Hyun-Ji,Sohn, Dong-Hwan,Song, Chi-Hyun The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.1

This investigation was undertaken to study the effects of oral administration (3 weeks) of Collybia confluens mycelial powder (CCMP) produced by a submerged culture on plasma glucose and other biochemical parameters in streptozotocin (STZ)-induced diabetic rats. Antidiabetic and hypolipidemic effects were proportionally increased with the increasing concentration of the CCMP for oral administration. The CCMP, at the dose of 400 mg/kg BW, substantially reduced the plasma glucose level by as much as 33.1 % as compared to the STZ-induced diabetic rats group. It also lowered the plasma total cholesterol, triglyceride, and low density lipoprotein (LDL) cholesterol by 22.9%, 19.9%, and 37.3%, respectively. The levels of total cholesterol and triglyceride in liver were reduced to the extent of by 13.5% and 18.8%, and the activity of alanine transaminase (ALT) and aspartate transaminase (AST) was decreased by 48.8% and 37.2%, respectively, under the influence of CCMP. The general components of CCMP were found to contain 26.18% carbohydrate, 3.67% crude ash, 4.02% crude fat, 22.55% crude protein, and 43.58% dietary fiber. The amino acid composition of the CCMP was also analyzed in detail.

Hypoglycemic and hypolipidemic effects of Allium victorialis leaf extract in high fat diet supplied mice

이영준,Ku, Sae-Kwang,Chung, In-Kwon,Cheon, Woo-Hyun,Lee, Young-Joon 대한예방한의학회 2011 대한예방한의학회지 Vol.15 No.1

The hypoglycemic and hypolipidemic effects of Allium victorialis (AV) leaf methanol extract were evaluated in a high fat diet (HFD) supplied mice. Changes on the serum glucose, total cholesterol, triglyceride, low density lipoprotein and high density lipoprotein were examined. The effects were compared with those of a group given 250 mg/kg of metformin. After 91 days of a continuous HFD supply, the mice were showed marked hyperglycemia and hyperlipemia. However, these hyperglycemia and hyperlipemia induced by the HFD were inhibited by the AV extract treatment at the three different doses (62.5, 125 and 250 mg/kg). The results suggest that the AV methanol extract is beneficial for improving the diet-induced hyperlipidemia and hyperglycemia in humans.

Experimental Study of Ginkgo-Chunghyul-dan on Anti-oxidant, Anti-platelet Aggregation, and Anti-hyperlipidemic Activity

윤상필,배형섭,박성욱,정우상,문상관,박정미,고창남,조기호,김용석 대한한의학회 2008 대한한의학회지 Vol.29 No.5

Objectives:Ginkgo-Chunghyul-dan (GCHD) is newly developed herbal medicine to prevent and treat stroke. In this study, we investigated whether the GCHD had antioxidant activity and anti-platelet aggregation effect in vitro and hypolipidemic activities in vivo. Methods:Anti-oxidant activity of GCHD was measured using the Blois method, anti-platelet effect of GCHD was assessed by the Born method, and hypolipidemic activities of GCHD were evaluated in corn oil- or Triton WR-1339-induced and cholesterol-fed rats. Results:GCHD showed anti-oxidant activity in the study inhibiting the formation of 1-diphenyl-2-picrylhydrazyl radicals and xanthine oxidase activity. GCHD had anti-platelet aggregation activity. GCHD significantly lowered total cholesterol (TC), triglyceride (TG), and low density lipoprotein cholesterol (LDL-C) in high cholesterol diet and Triton WR-1339 induced model TG in corn oil-induced model. GCHD had no acute toxicity at a single dosage. Conclusion:These results suggest that GCHD has the potential to treat hyperlipidemia and stroke. Objectives:Ginkgo-Chunghyul-dan (GCHD) is newly developed herbal medicine to prevent and treat stroke. In this study, we investigated whether the GCHD had antioxidant activity and anti-platelet aggregation effect in vitro and hypolipidemic activities in vivo. Methods:Anti-oxidant activity of GCHD was measured using the Blois method, anti-platelet effect of GCHD was assessed by the Born method, and hypolipidemic activities of GCHD were evaluated in corn oil- or Triton WR-1339-induced and cholesterol-fed rats. Results:GCHD showed anti-oxidant activity in the study inhibiting the formation of 1-diphenyl-2-picrylhydrazyl radicals and xanthine oxidase activity. GCHD had anti-platelet aggregation activity. GCHD significantly lowered total cholesterol (TC), triglyceride (TG), and low density lipoprotein cholesterol (LDL-C) in high cholesterol diet and Triton WR-1339 induced model TG in corn oil-induced model. GCHD had no acute toxicity at a single dosage. Conclusion:These results suggest that GCHD has the potential to treat hyperlipidemia and stroke.